A5045586189- Ocular Infections and Treatments
- Ocular Surface and Contact Lens
- Legionella and Acanthamoeba research
- Corneal surgery and disorders
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Corneal Surgery and Treatments
- Ocular Diseases and Behçet’s Syndrome
- RNA modifications and cancer
- Intraocular Surgery and Lenses
- Gut microbiota and health
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Inflammasome and immune disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Dermatology and Skin Diseases
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Rabies epidemiology and control
- Infectious Diseases and Mycology
- Click Chemistry and Applications
- Actinomycetales infections and treatment
- Amoebic Infections and Treatments
Beijing Tongren Hospital
2022-2025
Capital Medical University
2022-2025
Institute of Microbiology
2018-2022
University of Chinese Academy of Sciences
2018-2020
Chinese Academy of Sciences
2019-2020
Peking University
1989-2018
Abstract Gegen Qinlian Decoction (GQD), a traditional Chinese medicine (TCM) formula, has long been used for the treatment of common metabolic diseases, including type 2 diabetes mellitus. However, main limitation its wider application is ingredient complexity this formula. Thus, it critically important to identify major active ingredients GQD and illustrate mechanisms underlying action. Here, we compared effects berberine, hypothetical key pharmaceutical GQD, on diabetic rat model by...
To investigate the efficacy and mechanisms of human umbilical cord-derived MSC-derived extracellular vesicles (hucMSC-EVs) in a mouse model desiccation-induced dry eye disease (DED).hucMSC-EVs were enriched by ultracentrifugation. The DED was induced desiccating environment combined with scopolamine administration. mice divided into hucMSC-EVs group, fluorometholone (FML) PBS blank control group. Tear secretion, corneal fluorescein staining, cytokine profiles tears goblet cells,...
Purpose: Bacterial keratitis (BK) is a serious ocular infection that can cause severe inflammation and corneal scarring, leading to vision loss. In this study, we aimed investigate the involvement of ferroptosis in pathogenesis BK. Methods: Transcriptome analysis was performed evaluate ferroptosis-related gene expression human BK corneas. Subsequently, mouse models Pseudomonas aeruginosa stromal stem cells (CSSCs) were validated. The mice treated with levofloxacin (LEV) or combined...
Bacterial keratitis (BK) is the most common type of infectious keratitis. The spectrum pathogenic bacteria and their susceptibility to antibiotics varied with different regions. A meta-analysis was conducted review global culture rate, distribution, current trends, drug isolates from BK over past 20 years (2000-2020). Four databases were searched, published date limited between 2000 2020. Main key words "bacterial keratitis", "culture results" "drug resistance". Forty-two studies twenty-one...
Purpose: Fungal keratitis (FK) is a serious corneal infection with high morbidity. Host immune responses function as double-edged sword by eradicating fungal pathogens while also causing damage, dictating the severity, progression, and outcome of FK. However, underlying immunopathogenesis remains elusive. Methods: Time-course transcriptome was performed to illustrate dynamic landscape in mouse model Integrated bioinformatic analyses included identification differentially expressed genes,...
N6-methyladenosine (m6A), the most abundant internal mRNA modification, and N6,2’-O-dimethyladenosine (m6Am), found at first-transcribed nucleotide, are two examples of dynamic reversible epitranscriptomic marks. However, profiles distribution patterns m6A m6Am across different human mouse tissues poorly characterized. Here we report methylome through an extensive profiling 42 16 tissue samples. Globally, peaks in non-brain demonstrates mild tissue-specificity but correlated general, whereas...
Purpose: To clarify the clinical and imaging characteristics of Candida keratitis using in vivo confocal microscopy (IVCM) for improved early diagnosis management. Methods: A retrospective study 40 patients with at Beijing Tongren Hospital from January 2015 to December 2023 was conducted. Data included demographics, risk factors, assessments, lab tests, IVCM images. Ex methylene blue staining colonies were also analyzed complement findings. Results: Key factors identified topical steroid...
Purpose: Fungal keratitis caused by Aspergillus flavus (A. flavus) can result in severe inflammation and corneal stromal melting, leading to visual impairment. This study aimed identify virulent genes correlated with the severity of A. using whole-genome sequencing. Methods: Whole-genome sequencing 21 clinical strains from cornea was performed elucidate pathogenesis infectious keratitis, followed pan-genome analysis virulence analysis. To further understand results previous analyses, growth...
Bacterial keratitis (BK) is a severe ocular infection that can lead to vision loss, with antimicrobial resistance (AMR) posing growing challenge. This study retrospectively analyzed 1071 bacterial isolates from corneal infections over 10-year period (2014–2024) at tertiary ophthalmic center in Beijing, categorizing them into three distinct phases: pre-COVID-19, during COVID-19, and post-COVID-19. The results indicated significant changes pathogen distribution, including marked decrease...
Objective This study investigated the susceptibility of various Fusarium fungi to five topical antifungal agents: natamycin, voriconazole, chlorhexidine, natamycin combined with and voriconazole chlorhexidine. And explore clinical feasibility combination therapy in treatment corneal infections caused by F. solani , goal optimizing regimen for fungal keratitis. Methods A total 194 strains were isolated from corneas between 2013 2024 identified species level using mass spectrometry. The MICs...
The limbal niche supports the self-renewal of epithelial stem cells (LESCs). corneal stromal cell (CSSC) is an important component in that regulates LESC phenotype. However, intercellular communication between LESCs and CSSCs has yet to be elucidated.A traditional two-dimensional (2D) system, a direct three-dimensional (3D) indirect 3D coculture system were used elucidate paracrine pathway effect on LESCs. To reveal impact CSSC derived extracellular vesicles (CSSC-EVs) LESCs, GW4869 CSSC-EVs...
To analyze the epidemiologic features, culture positivity, fungal spectrum of different sites ocular infection in North China over 20 years from 2001 to 2020.11, 635 patients suspected were reviewed. The demographic profile, positive rate among sites, distribution, and trends main pathogens cornea intraocular fluid analyzed.Among 11, samples, was 23.6%. Most samples (83.1%) cornea, their positivity 26.9%. Fungal keratitis occurred more often during harvesting season (October December; 34.0%)...
Purpose: To investigate the relationship between Acanthamoeba genotypes, clinical manifestations, and outcomes in keratitis (AK) patients. Methods: This retrospective study included 159 culture-confirmed AK Patients’ data were collected, including demographics, initial diagnosis, treatments, features. The genotype of was identified through sequencing Diagnostic Fragment 3 (DF3) region small ribosomal subunit RNA genes. phylogenetic tree constructed using ClustalW model maximum likelihood...
Purpose: This study aimed to investigate corneal limbus changes in patients with type 2 diabetes mellitus (DM) using vivo confocal microscopy (IVCM) and explore the correlation between their ocular manifestations systemic status. Methods: Fifty-five DM 20 age-matched controls were included. The following IVCM parameters compared groups: palisades of Vogt (POV), epithelial thickness (CET), basal cell density (BCD), subbasal nerve plexus, dendritic density. All subjects underwent blood urine...
Acanthamoeba keratitis (AK) is a blinding corneal infection caused by the protozoan Acanthamoeba. The long-term course of AK suggests host immunity could not kill rapidly. immune status still unclear in late stage AK. comparative transcriptome analysis was made based on bulk RNA sequencing cornea tissues from patients and donors. Differentially expressed genes enriched signaling pathways were calculated. CIBERSORT algorithm used for infiltration tissue between normal controls. A total 2668...
Purpose: Nocardia keratitis is a serious and sight-threatening condition. This study aims to reveal the virulence antimicrobial resistance gene profile of strains using whole genome sequencing. Methods: Whole-genome sequencing was performed on 23 cornea-derived strains. Together with genomic data from respiratory tract environment, 141 genomes were then utilized for phylogenetic pan-genome analyses, followed by antibiotic analysis. The correlations between genes pathogenicity experimentally...
Purpose: Our aim was to investigate risk factors, clinical characteristics, and antibiotic susceptibility patterns of cornea-isolated Streptococcus species collected at a tertiary hospital in China over 18 years. Methods: This retrospective study reviewed data from 350 patients diagnosed with Streptococcal keratitis Beijing Tongren Hospital between January 2006 December 2023, including demographics, signs, vivo confocal microscopy (IVCM) imaging, testing. Results: The predominant type...