A5085658941- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis Viruses Studies and Epidemiology
- Infant Nutrition and Health
- COVID-19 Impact on Reproduction
- Liver physiology and pathology
- Liver Disease and Transplantation
- Breastfeeding Practices and Influences
- Pharmacological Effects of Natural Compounds
- Virus-based gene therapy research
- Phytochemistry and Bioactivity Studies
- SARS-CoV-2 and COVID-19 Research
- Pancreatic function and diabetes
- Global Maternal and Child Health
- Advanced Multi-Objective Optimization Algorithms
- Pancreatic and Hepatic Oncology Research
- Optimal Experimental Design Methods
- Neonatal Health and Biochemistry
- Virology and Viral Diseases
- Trace Elements in Health
- Cancer-related Molecular Pathways
- Liver Diseases and Immunity
- Immune responses and vaccinations
- Genetic Syndromes and Imprinting
Peking University
2014-2024
Rockefeller University
2016-2017
Beijing Polytechnic
1988
Beijing University of Technology
1988
Keep hepatocytes fresh Freshly isolated primary human (PHHs) quickly lose their identity and function in vitro, limiting application modeling infectious liver diseases screening drugs. Xiang et al. describe a simple effective five-chemical culture condition that can maintain the mature of cultured PHHs for an extended period time. this way support entire viral life cycle recapitulate long-term infection hepatitis B virus. This system created useful drug-screening platform developing new...
The COVID-19 global epidemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a great public health emergency. Discovering antiviral drug candidates urgent for the prevention and treatment of COVID-19. This work aims to discover natural SARS-CoV-2 inhibitors from traditional Chinese herbal medicine licorice. We screened 125 small molecules Glycyrrhiza uralensis Fisch. (licorice, Gan-Cao) virtual ligand screening targeting receptor-binding domain (RBD) spike protein....
Abstract The discovery of sodium taurocholate cotransporting polypeptide (NTCP) as the hepatitis B virus (HBV) receptor enabled researchers to create hepatoma cell lines susceptible HBV infection. Infection in current systems, however, is inefficient and fails spread. efficiency enhanced by treating cells with polyethylene glycol 8000 (PEG) during However, this alone does not promote Here we show that maintaining PEG culture medium increases rate infection at least one order magnitude, and,...
The global pandemic of COVID-19 caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection confers great threat to public health. Human breast milk is a complex nutritional composition nourish infants and protect them from different kinds infectious diseases including COVID-19. Here, we identified that lactoferrin (LF), mucin1 (MUC1), α-lactalbumin (α-LA) human inhibit SARS-CoV-2 using pseudovirus system transcription replication-competent virus-like-particles...
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Terminally differentiated cells can be generated by lineage reprogramming, which is, however, hindered incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy mimicking the natural regeneration route, permits generating expandable hepatic progenitor functionally competent human hepatocytes. Fibroblasts were first induced into progenitor-like (hHPLCs), could robustly expand in vitro efficiently engraft vivo....
Mutations in HBV core promoter (CP) are suggested to affect viral replication and disease progression. We investigated CP deletion/insertion mutations (Del/Ins) hepatitis B e antigen (HBeAg)-positive chronic (CHB) patients before during antiviral treatment.Direct clone sequencings were used for detection of Del/Ins 12 patients. The dynamic changes tracked these cases until week 48 treatment. effects on activities X protein (HBx) analysed using luciferase assay sequence comparison,...
Currently, human health due to corona virus disease 2019 (COVID-19) pandemic has been seriously threatened. The coronavirus severe acute respiratory syndrome 2 (SARS-CoV-2) spike (S) protein plays a crucial role in transmission and several S-based therapeutic approaches have approved for the treatment of COVID-19. However, efficacy is compromised by SARS-CoV-2 evolvement mutation. Here we report S receptor-binding domain (RBD) inhibitor licorice-saponin A3 (A3) could widely inhibit RBD...
The detailed understanding of fibrogenesis has been hampered by a lack important functional quiescence characteristics and an in vitro model to recapitulate hepatic stellate cell (HSC) activation. In our study, we establish robust endoderm- mesoderm-sourced quiescent-like induced HSCs (iHSCs) derived from human pluripotent stem cells. Notably, iHSCs present features mature HSCs, including accumulation vitamin A the lipid droplets maintained quiescent features. addition, display fibrogenic...
Neural tube defects (NTDs) are a group of common and severe congenital malformations. The PI3K-AKT signalling pathway plays crucial role in the neural development. There is limited evidence concerning any possible association between aberrant methylation genes NTDs. Therefore, we aimed to investigate potential associations Methylation studies utilizing microarray genome-methylation data derived from tissues ten NTD cases eight non-malformed controls were performed. Targeted DNA analysis was...
Genetic variability has significant impacts on biological characteristics and pathogenicity of hepatitis B virus (HBV), in which the N-terminal sequence presurface 1 (preS1) region HBV large surface protein (LHBs) displays genotype (GT) dependent genetic heterogeneity. However, influence this heterogeneity its roles is largely unknown. By analyzing 6560 full-length genome sequences GTA-GTH downloaded from HBVdb database, preS1 were divided into four representative types, namely C-type...
Occult HBV infection (OBI) could have serious clinical consequences in patients receiving immunosuppressive therapy. We aimed to investigate the prevalence of OBI Chinese with autoimmune hepatitis (AIH) and analyze its virological features. 103 AIH cases were enrolled. Hepatitis B virus (HBV) serological markers screened by chemiluminescence. HBV-DNA detected nest-PCR real-time PCR. genotyping mutation analysis performed Sanger sequencing. Twenty-four out (23.30%) had as evidenced positive...
Chronic hepatitis B virus (HBV) infection, referred to the presence of surface antigen (HBsAg) over 6 months, is a serious health issue because its severe sequelae such as cirrhosis and hepatocellular carcinoma. Prevention mother-to-child transmission (MTCT) HBV most important control vast majority acquisition in infancy will become chronic carriers. Since availability immunoglobulin (HBIG) 1970s vaccine 1980s, China's central local authorities have made tremendous efforts prevent MTCT HBV.1...
To facilitate adeno-associated virus (AAV)-mediated gene therapy in China, we conducted a study on the distribution of AAV-neutralizing antibodies (NAbs) healthy subjects and patients with Duchenne muscular dystrophy (DMD)/Becker (BMD). A total 352 adult controls (ACs) from national multicenter study, 100 schoolchild (SCs), 281 DMD/BMD Peking Union Medical College Hospital were enrolled this study. Cell-based inhibition assays applied, serum samples demonstrating 50% infection considered...
Despite the presence of hepatitis B virus (HBV) in human breastmilk mothers infected with HBV, it has been shown that breastfeeding does not increase risk mother-to-child transmission (MTCT) HBV. We tested hypothesis may contain active components bind to HBV and inhibit infectivity The results show whey significantly inhibited binding surface antigen (HBsAg) its antibodies competitive inhibition immunoassays. far-western blotting showed HBsAg bound a protein 80 kD whey, which was identified...
Background High genetic variability at the reverse transcriptase (RT) region of HBV could confer resistance to nucleoside/nucleotide analogues (NUCs). The aim this study was identify new RT amino acid (AA) substitutions related NUC resistance. Methods sequences genotype C from 501 chronic hepatitis B (CHB) patients were analysed potential In vitro studies without and with NUCs performed in a HepG2 cell line transfected by clones harbouring wild-type or substituted AA(s) interest. Results...