A5062141566- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Mast cells and histamine
- Urticaria and Related Conditions
- Artificial Immune Systems Applications
- Pediatric health and respiratory diseases
- Biochemical Analysis and Sensing Techniques
- Receptor Mechanisms and Signaling
- Transgenic Plants and Applications
- Cell Adhesion Molecules Research
- Neuroendocrine regulation and behavior
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- ICT in Developing Communities
- Community Development and Social Impact
- Monoclonal and Polyclonal Antibodies Research
- Service-Learning and Community Engagement
- Olfactory and Sensory Function Studies
- COVID-19 diagnosis using AI
- Immunodeficiency and Autoimmune Disorders
McMaster University Medical Centre
2018-2025
McMaster University
2020-2025
Stanford University
2023-2024
Population Health Research Institute
2024
Hamilton College
2023
Allergen-specific immunoglobulin E (IgE) antibodies mediate pathology in diseases such as allergic rhinitis and food allergy. Memory B cells (MBCs) contribute to circulating IgE by regenerating IgE-producing plasma upon allergen encounter. Here, we report a population of type 2–polarized MBCs defined CD23 hi , IL-4Rα CD32 low at both the transcriptional surface protein levels. These MBC2s are enriched IgG1- IgG4-expressing while constitutively expressing germline transcripts for IgE. from...
The COVID-19 pandemic has sparked unprecedented public health and social measures (PHSM) by national local governments, including border restrictions, school closures, mandatory facemask use stay at home orders. Quantifying the effectiveness of these interventions in reducing disease transmission is key to rational policy making response current future pandemics. In order estimate interventions, detailed descriptions their timelines, scale scope are needed. Health Intervention Tracking for...
Abstract Background The generation of IgE‐mediated food allergy in humans is silent and only diagnosed upon manifestation clinical symptoms. While experimental models have been used to investigate some mechanisms allergic sensitization, the humoral immunity memory remains be elucidated. Here, we defined evolution allergen‐specific B‐cell responses during epicutaneous sensitization foods. Methods Wild‐type genetic knockout animals, drug or antibody strategies for cell depletion immunoglobulin...
Abstract Allergen-specific IgE antibodies mediate allergic pathology in diseases such as rhinitis and food allergy. Memory B cells (MBCs) contribute to circulating by regenerating IgE-producing plasma upon allergen encounter. We report a population of type 2 polarized MBCs defined CD23 hi , IL-4Rα CD32 low at the transcriptional surface protein levels. These “MBC2s” are enriched IgG1 IgG4-expressing cells, while constitutively expressing germline transcripts for IgE. from patients with...
ABSTRACT Allergic reactions to foods are primarily driven by allergen‐binding immunoglobulin (Ig)E antibodies. IgE‐expressing cells can be generated through direct switching from IgM IgE or a sequential class pathway where activated B first switch an intermediary isotype, most frequently IgG1, and then IgE. It has been proposed that recombination is involved in augmenting the severity of allergic reactions, generating high affinity IgE, differentiation plasma cells, holding memory responses....
Mast cells drive the inappropriate immune response characteristic of allergic inflammatory disorders via release pro-inflammatory mediators in to environmental cues detected by IgE-FcεRI complex. The role TGF-β-activated kinase 1 (TAK1), a participant related signaling other contexts, remains unknown allergy. We detect novel activation TAK1 at Ser412 IgE-mediated under SCF-c-kit potentiation mast cell-driven inflammation, which is potently blocked inhibitor 5Z-7-oxozeaenol (OZ). We,...
Abstract Allergic reactions to foods are driven by allergen-binding immunoglobulin (Ig)E antibodies. IgE- expressing cells can be generated through a sequential class switching pathway where activated B first switch an intermediary isotype, most frequently IgG1, and then IgE. It has been proposed that recombination is important in generating high affinity IgE, augmenting anaphylactic reactions, holding the memory of IgE responses. Here, we observed surprising redundancy IgG1 for functional...
Abstract Long-lived immunoglobulin (Ig) E responses against innocuous environmental and dietary antigens (Ags) are maintained by an IgG1-dominant memory B cell (MBC) compartment primed for IL-4 responsiveness. In this work, we investigated the plasticity of MBC fated IgE class switch recombination upon Ag re-exposure. Antibody-mediated IL-4R( blockade augmented germinal center response uncovered IL-4/IL-13 dependency within type 2 (MBC2) skewed phenotype. absence signaling during recall...
Abstract Immunoglobulin (Ig) E is the critical effector molecule in allergic reactions. Consequently, research efforts to understand biology of IgE-expressing cells paramount importance. In particular, role IgE + memory B (MBCs) perpetuation reactivity has been subject intense research. Studies mice have convincingly established that are rare and transient and, therefore, an unlikely candidate maintain disease. contrast, MBCs detected by flow cytometry sputum peripheral blood humans proposed...
Abstract IgE production against innocuous antigens can lead to life-threatening reactions such as anaphylaxis. While levels drastically decline with strict allergen avoidance, the ability regenerate persist for a lifetime, is case peanut allergy. The mechanism by which regenerates remains unresolved. A novel culture system and application of single-cell RNA-sequencing, elucidated transcriptomic signature human peanut-reactive B T cells revealed IL-4/IL-13 signaling pathway critical recall...