Recent studies have revealed that microRNAs (miRs) play important roles in the regulation of angiogenesis. In this study, we characterized miR-382 upregulation by hypoxia and functional relevance tumor miRs induced MKN1 human gastric cancer cells were investigated using miRNA microarrays. We selected found expression was regulated HIF-1α. Conditioned media (CM) from transfected with a inhibitor (antagomiR-382) under hypoxic conditions significantly decreased vascular endothelial cell (EC)...

Objective The NLRP 3 inflammasome is closely linked to the pathophysiology of a wide range inflammatory diseases. This study was undertaken identify small molecules that directly bind in order develop pharmacologic interventions for 3‐related Methods A structure‐based virtual screening analysis performed with ~62,800 compounds select efficient inhibitors. production caspase 1‐p10 and interleukin‐1β ( IL ‐1β) measured by immunoblotting enzyme‐linked immunosorbent assay examine activation. Two...

Airborne particulate matter with a diameter of < 10 µm (PM10) causes oxidative damage, inflammation, and premature skin aging. In this study, we evaluated whether polyphenolic antioxidants attenuate the inflammatory responses PM10-exposed keratinocytes.Primary human epidermal keratinocytes were exposed in vitro to PM10 absence or presence punicalagin (-)-epigallocatechin-3-gallate (EGCG), which are major found pomegranate green tea, respectively. Assays performed determine cell viability,...

Abnormal deposition of melanin may cause an aesthetic skin problem; therefore, the control unwanted excessive synthesis is major goal cosmetic research.To identify novel tyrosinase (TYR) inhibitors from marine plants and examine their cellular antimelanogenic effects.The extracts 50 endemic to Korea were screened against human TYR. Active constituents then isolated selected plant that showed potential chemical structures elucidated. Furthermore, effects examined using murine melanoma B16/F10...

Epidemiological and experimental evidence support health risks associated with the exposure to airborne particulate matter a diameter of &lt;10 μ M (PM10). PM10 stimulates production reactive oxygen species (ROS) inflammatory mediators. Thus, we assumed that natural antioxidants might provide benefits attenuating hazardous effects PM10. In present study, examined pomegranate peel extract (PPE) on THP‐1 monocytic cells exposed induced cytotoxicity ROS. It also increased expression secretion...

Airborne particulate matter can cause oxidative stress, inflammation, and premature skin aging. Marine plants such as Ecklonia cava Kjellman contain high amounts of polyphenolic antioxidants. The purpose this study was to examine the antioxidative effects E. extract in cultured keratinocytes exposed airborne with a diameter &lt;10 μ m (PM10). After exposure HaCaT PM10 absence presence its constituents, cell viability cellular lipid peroxidation were assessed. eckol dieckol on cytokine...

Ultraviolet (UV) radiation induces oxidative injury and inflammation in human skin. Scutellaria radix (SR, the root of baicalensis Georgi) contains flavonoids with high UV absorptivity antioxidant properties. The purpose this study was to examine potential use SR extract as an additive cosmetic products for protection. its butanol (BuOH) fraction strongly absorbed displayed free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl radials...

Ultraviolet (UV) radiation-induced loss of dermal extracellular matrix is associated with skin photoaging. Recent studies demonstrated that keratinocyte-releasable stratifin (SFN) plays a critical role in collagen metabolism by inducing metalloproteinase 1 (MMP1) expression target fibroblasts. In the present study, we examined whether SFN released from UVB-irradiated epidermal keratinocytes increases MMP1 release fibroblasts, and these events are affected p-coumaric acid (p-CA), natural...

Antioxidants with antimelanogenic activity are potentially useful for the attenuation of skin hyperpigmentation disorders. In a previous study, luteolin 7-sulfate isolated from Phyllospadix iwatensis Makino, marine plant, was shown to inhibit cellular melanin synthesis. The aim present study examine its action mechanism, focusing on regulation tyrosinase (TYR) expression in cells. Cell-based assay undertaken using murine melanoma B16-F10 cells and primary human epidermal melanocytes (HEMs)....

Abstract Objectives Resveratryl triglycolate ( RTG ) is a hybrid compound derived by the esterification of resveratrol with glycolic acid. This has been previously shown to inhibit cellular melanin synthesis in vitro . study aimed examine vivo skin‐depigmenting efficacy human participants. Methods In total, 22 women aged between 25 and 49 years Fitzpatrick skin type III or IV were enrolled. Their forearms exposed UV induce artificial pigmentation. The test product containing 0.4% control was...

Antimelanogenic peptides are potentially useful to treat hyperpigmentation, but many have limited application because of high cost and/or low activity. To identify small and potent peptide inhibitors cellular melanin synthesis that for cosmetic medical applications. A positional scanning synthetic tetrapeptide combinatorial library was used screening active peptides. activities the pools individual were evaluated in B16‐F10 melanoma cells human epidermal melanocytes treated with...

Oxidized phospholipids are well known to play physiological and pathological roles in regulating cellular homeostasis disease progression. However, their role cancer metastasis has not been entirely understood. In this study, effects of oxidized phosphatidylcholines such as 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine (POVPC) on epithelial-mesenchymal transition (EMT) autophagy were determined cells by immunoblotting confocal analysis. Metastasis was analyzed a scratch wound...

Activation of the NLRP3 inflammasome is a necessary process to induce fibrosis in nonalcoholic fatty liver disease (NAFLD). Nonalcoholic steatohepatitis (NASH) kind NAFLD that encompasses spectrum disease. It characterized by inflammation and ballooning hepatocytes during steatosis. We tested whether inhibiting could prevent development pathology NASH. identified loganin as an inhibitor investigated vivo administration prevented NASH symptoms using methionine-choline deficient (MCD) diet...

Atopic dermatitis is a chronic inflammatory skin disease, of which incidence closely related to exposure environmental pollutants and allergens. Thymic stromal lymphopoietin (TSLP) plays an important role in the early stages atopic development by inducing Th2 immune responses. In addition, TSLP regulates activation group 2 innate lymphoid cells (ILC2), promoting pathogenesis dermatitis. The aim this study was investigate whether celastrol alleviated symptoms regulating expression ILC2...

Inflammation and immunity are linked to the onset development of obesity metabolic disorders. Pattern recognition receptors (PRRs) key regulators inflammation in response infection stress, they have critical roles metainflammation. In this study, we investigated whether RIG-I (retinoic acid-inducible gene I)-like were involved regulation obesity-induced stress knockout (KO) mice fed a high-fat diet (HFD). KO an HFD for 12 weeks showed greater body weight gain, higher fat composition, lower...

Ultraviolet radiation (UV) is a major cause of photoaging, which also involves inflammatory cytokines and matrix metalloproteinases (MMP). The present study was undertaken to examine the UVB‐protecting effects yellow‐colored plant extracts in cell‐based assays. HaCaT keratinocytes were exposed UVB absence or presence extracts, resulting changes cell viability cytokine expression measured. Of tested, Gardenia jasminoides extract showed lowest cytotoxicity dose‐dependently enhanced viabilities...

자외선은 피부 광노화의 주요 요인이며, 효과적인 자외선 차단제가 피부의 건강과 아름다움을 위해 필요하다. 본 연구는 세포 실험을 통하여 자외선에 의해 유도된 사멸, 산화적 스트레스, matrix metalloproteinase 1 발현에 미치는 죽여추출물의 영향을 알아보고자 수행하였다. HaCaT 인간 각질세포를 여러 농도의 죽여추출물 유무 조건에서 자외선을 조사하고 세포의 생존율과 생화학적 과정들의 변화를 분석하였다. 죽여추출물은 조사한 생존율을 증진시켰고, procaspase 3가 활성화 형태로 절단되고, Bax/Bcl-2 비율이 증가하는 자살 과정을 완화시켰다. 노출된 세포에서 활성산소의 발생과 지질 과산화도 감소시켰다. 또한 자극된 1의 발현과 c-Jun N-terminal kinase의 인산화를 억제하였다. 죽여추출물이 의한 그리고 발현을 억제함을 보여 주었으며, 이 추출물이 일부 현상을 억제하는 화장품 원료로 유용함을 시사하였다. Ultraviolet...