A5026034873- Neuroscience of respiration and sleep
- Thermal Regulation in Medicine
- Fullerene Chemistry and Applications
- Neuropeptides and Animal Physiology
- Nitric Oxide and Endothelin Effects
- Biochemical effects in animals
- Neurological Disease Mechanisms and Treatments
- Chemical Reactions and Isotopes
- Anesthesia and Neurotoxicity Research
- Mesenchymal stem cell research
- Immune Response and Inflammation
- Neurological Disorders and Treatments
- Electron Spin Resonance Studies
- Neuroscience and Neuropharmacology Research
- Cancer therapeutics and mechanisms
- Vagus Nerve Stimulation Research
- Respiratory Support and Mechanisms
- Tendon Structure and Treatment
- Neurogenesis and neuroplasticity mechanisms
- Neuroendocrine regulation and behavior
- Eicosanoids and Hypertension Pharmacology
- Pluripotent Stem Cells Research
- Receptor Mechanisms and Signaling
- Dendrimers and Hyperbranched Polymers
- Carbon Nanotubes in Composites
Institute of Physiology National Academy of Sciences of Belarus
2015-2025
National Academy of Sciences of Belarus
1994-2023
University of Glasgow
2023
Association of Research Libraries
2023
University of Hong Kong
2023
Florida Institute of Technology
2023
Environment and Health Group (United States)
2023
Kazan Federal University
2023
Czech Academy of Sciences, Institute of Physiology
1994-2021
B.I. Stepanov Institute of Physics
2021
Experimentally, systemic inflammation induced by a bolus intravenous injection of lipopolysaccharide (LPS) may be accompanied three different thermoregulatory responses: monophasic fever (the typical response to low doses LPS), biphasic (medium doses), and hypothermia (high doses). In our recent study [Romanovsky, A. A., V. Kulchitsky, C. T. Simons, N. Sugimoto, M. Szekely. Am. J. Physiol. (Regulatory Integrative Comp. Physiol.). press], did not occur in subdiaphragmatically vagotomized...
The natural switch from fever to hypothermia observed in the most severe cases of systemic inflammation is a phenomenon that continues puzzle clinicians and scientists. present study was first evaluate direct experiments how development vs. during forms impacts pathophysiology this malady mortality rates rats. Following administration bacterial lipopolysaccharide (LPS; 5 or 18 mg/kg) clinical Escherichia coli isolate (5 × 10(9) 1 10(10) CFU/kg), developed rats exposed mildly cool...
Recent evidence has suggested a role of abdominal vagal afferents in the pathogenesis febrile response. The vagus consists five main branches (viz., anterior and posterior celiac branches, gastric hepatic branch). branch responsible for transducing pyrogenic signal from periphery to brain not as yet been identified. In present study, we address this issue by testing responsiveness male Wistar rats subjected one four selective vagotomies: (CBV), (GBV), (HBV), or sham (SV). case CBV, GBV, HBV,...
We hypothesized that the systemic inflammatory response undergoes two consecutive stages, each characterized by different nonspecific sickness patterns. To test this hypothesis, we studied thermal, nociceptive, and motor responses to lipopolysaccharide (LPS) in 43 unanesthetized, habituated, lightly restrained male Wistar rats previously implanted with a catheter jugular vein. Escherichia coli LPS was injected intravenously dose of 0, 0.1, 1, 10, 100, or 1,000 micrograms/kg. Colonic...
This study explains why the recently described triphasic lipopolysaccharide (LPS) fevers have been repeatedly mistaken for biphasic fevers. Experiments were performed in loosely restrained male Wistar rats with a catheter implanted into right jugular vein. Each animal was injected Escherichia coli LPS, and its colonic (T c ) tail skin temperatures monitored. The results are presented as time graphs phase-plane plots; latter case rate of change T is plotted against . At an ambient temperature...
The article presents the calculation of structural and electronic characteristics a conjugate consisting drug carboplatin (C 6 H 12 N 2 O 4 Pt) fullerenol 60 (OH) 24 ) nanocarrier by HF-3c method. Optimization geometric structure in an aqueous medium was carried out using same level theory polarizable continuum model. To clarify nature intermolecular bond assess strength interaction between molecule fullerenol, topological analysis mechanisms non-covalent performed based on quantum atoms...
The structure and physicochemical properties of scaffolds obtained from collagen gel using connective tissue sheaths paravertebral tendons Wistar rats were studied. at 37 °C (filmy) 6 (volumetric). During hardening, the form globular extraglomerular fractions, which is typical for gels tendon membranes. ratio fraction volumes determined by pore kinking fibrils. In filmy scaffold, closed-type pores with weakened are formed, leads to dominance scaffold. volumetric intensified, open-type...
This paper disproves the common belief that all doses of lipopolysaccharide (LPS) are commonly referred to as biphasic fever inducing (≥2 μg/kg) cause truly responses. A catheter was implanted into right jugular vein several strains adult male rats, and animals were habituated experimental conditions. At an ambient temperature 30.0°C, loosely restrained injected with a 10 μg/kg dose LPS (various preparations), their colonic (T c ) tail skin temperatures monitored (from ≥1 h before ≥7 after...
Research concerning new targeting delivery systems for pharmacologically active molecules and genetic material is of great importance. The aim the present study was to investigate potential fourth generation (P4) cationic phosphorus-containing dendrimers bind fluorescent probe 8-anilino-1-naphthalenesulfonate (ANS), anti-neoplastic drug cisplatin, anti-HIV siRNA siP24 its capability deliver green protein gene (pGFP) into cells. interaction between P4 ANS (as model drug) investigated. binding...
Neuroepithelial tumor cells were cultured in vitro. The biopsy material was taken from 93 children at removal of the brain tumors during neurosurgical operations. individual features sensitivity primary cultures respect to protocol-approved chemotherapy drugs and changes Interleukin-6 (Il-6) level culture medium after application established. initial Il-6 exceeded 600.0 pg/ml cultural with histologically verified pilomyxoid astrocytoma cells, ranged 100.0 200.0 cultivation...
The repeatedly observed attenuation of fever in vagotomized rats has been accepted as evidence an essential role vagal afferents the transduction pyrogenic signals from periphery to brain. If, however, general condition a animal is poor (the usual case) and accompanied by malnutrition body mass loss (common complications vagotomy), febrile responsiveness can be suppressed not because lack afferentation, but rather secondarily malnutrition-associated thermogenic incompetence. In present...
The limited specificity of nanoparticle (NP) uptake by target cells associated with a disease is one the principal challenges nanomedicine. Using threshold mechanism plasmonic nanobubble (PNB) generation and enhanced accumulation clustering gold nanoparticles in cells, we increased PNB detection versus non-target more than order magnitude compared to NP same cells. This improved cellular PNBs was demonstrated six different cell models representing diverse molecular targets such as epidermal...
Lipopolysaccharide (LPS)-induced systemic inflammation is accompanied by either hypothermia (prevails when the ambient temperature (Ta) subneutral) or fever Ta neutral higher). Because platelet-activating factor (PAF) a proximal mediator of LPS inflammation, it should mediate both thermoregulatory responses to LPS. That PAF possesses hypothermic activity and mediates LPS-induced known. We asked whether pyrogenic (Expt 1) 2). The study was conducted in Long-Evans rats implanted with jugular...
Systemic inflammatory response syndrome is associated with either fever or hypothermia, but the mechanisms responsible for switching from one to other are unknown. In experimental animals, systemic inflammation often induced by bacterial lipopolysaccharide (LPS). To identify diencephalic and brainstem structures involved in fever-hypothermia switch, we studied expression of c-Fos protein, a marker neuronal activation, rats treated same high dose LPS (0.5 mg/kg, intravenously) thermoneutral...
Subdiaphragmatically vagotomized rats cannot mount a febrile response to pyrogens and are believed have severe thermoregulatory deficiencies. We addressed the issue of thermoeffector competence by asking three questions. In Expt. 1 we asked, can readily recruit tail skin vasoconstriction in course moderate cold exposure? 2 question was, brown adipose tissue (BAT) thermogenesis be activated (e.g., pinch)? 3, investigated question: elevate their body temperature ephedrine (a drug high...
In the era of increasing effectiveness treatment methods and drugs used in modern neuro-oncology, targeted delivery diagnostic medicinal substances to tumor is great importance. The aim work study silico optimal rational approaches creation nanocontainers for drug delivery. Here we present results DFT simulation molecular electronic structure, as well possible mechanisms formation water-soluble conjugate cytotoxic cisplatin (cis-[Pt(NH3)2Cl2]) fullerenol (C60(OH)24). calculations were...
The review is dedicated to the analysis of modern nanotechnologies, presented in patents and research articles aimed at optimization cancer diagnostics treatment. attention focused on dendrimers, fullerenes heterocyclic compounds with antitumor effect. mechanisms activity nanoparticles manifest intracellular, cellular, tissue, organ system levels. Such diverse effect from DNA mitochondria level immune neurohumoral defense reactions perplexes development targeted agents. authors propose pay...