A5038567552- Banana Cultivation and Research
- Papaya Research and Applications
- Cassava research and cyanide
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Plant Micronutrient Interactions and Effects
- Advanced Proteomics Techniques and Applications
- Genetics and Plant Breeding
- Plant Stress Responses and Tolerance
- Agricultural and Food Sciences
- Plant Molecular Biology Research
- Plant Pathogenic Bacteria Studies
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Monoclonal and Polyclonal Antibodies Research
- Analytical Chemistry and Chromatography
- Neuroscience and Neuropharmacology Research
- Biosimilars and Bioanalytical Methods
- Nematode management and characterization studies
- Protein purification and stability
- Soil and Land Suitability Analysis
- Livestock and Poultry Management
- Glycosylation and Glycoproteins Research
- Biosensors and Analytical Detection
University of Arkansas System
2025
Universidade Federal do Recôncavo da Bahia
2024
Brazilian Agricultural Research Corporation
2018-2023
Thermo Fisher Scientific (United States)
2023
Pharmaceutical Product Development (United States)
2014-2022
Neurological Research Institute
2021
Texas Children's Hospital
2021
Baylor College of Medicine
2020
State University of Norte Fluminense
2017-2020
Milliman (United States)
2020
Reactive oxygen species produced by RBOHD mediate rapid, long-distance stress signals in plants.
Within their natural habitat plants are subjected to a combination of different abiotic stresses, each with the potential exacerbate damage caused by others. One most devastating stress combinations for crop productivity, which frequently occurs in field, is drought and heat stress. In this study we conducted proteomic metabolic analysis Arabidopsis thaliana We identified 45 proteins that specifically accumulated response combination. These included enzymes involved reactive oxygen...
Abstract Being sessile organisms, plants evolved sophisticated acclimation mechanisms to cope with abiotic challenges in their environment. These are activated at the initial site of exposure stress, as well systemic tissues that have not been subjected stress (termed acquired [SAA]). Although SAA is thought play a key role plant survival during little known about signaling underlying it. Here, we report requires least two different signals: an autopropagating wave reactive oxygen species...
Abstract Arabidopsis thaliana GAMT1 and GAMT2 encode enzymes that catalyze formation of the methyl esters gibberellins (GAs). Ectopic expression or in Arabidopsis, tobacco (Nicotiana tabacum), petunia (Petunia hybrida) resulted plants with GA deficiency typical phenotypes, such as dwarfism reduced fertility. are both expressed mainly whole siliques (including seeds), peak transcript levels from middle until end silique development. Within siliques, was previously shown to be mostly...
An AccQ*Tag ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (AccQ*Tag-UPLC-ESI-MS/MS) method for fast, reproducible, and sensitive amino acid quantitation in biological samples, particularly, the malaria parasite Plasmodium falciparum is presented. The Waters Acquity TQD UPLC/MS system equipped with a photodiode array (PDA) detector was used separation detection. developed validated using standard mixtures containing acidic, neutral, basic acids. For...
It is well known that significant metabolic change take place as cells are transformed from normal to malignant. This review focuses on the use of different bioinformatics tools in cancer metabolomics studies. The article begins by describing technologies and data generation techniques. Overview pre-processing techniques provided multivariate analysis discussed illustrated with case studies, including principal component analysis, clustering techniques, self-organizing maps, partial least...
Metabolomics is the methodology that identifies and measures global pools of small molecules (of less than about 1,000 Daltons) a biological sample, which are collectively called metabolome. can therefore reveal metabolic outcome genetic or environmental perturbation regulatory network, thus provide insights into structure regulation network. Because chemical complexity metabolome limitations associated with individual analytical platforms for determining metabolome, it currently difficult...
Background: Biotherapeutics development requires validated assays in biological matrices for pharmacokinetic assessment. Historically, ligand-binding have been the predominant platform available. Recently, alternative hybrid methods, combining analyte enrichment with LC–MS detection emerged. Methodology & results: The validation of an immunoaffinity (IA)-LC–MS/MS method to quantify a monoclonal antibody biotherapeutic cynomolgus monkey serum is described. This includes capture serum,...
Manual phenotyping for papaya Carica (L) breeding purposes limits the evaluation of a great number plants and hampers selection superior genotypes. This study aimed to validate two methodologies morpho-agronomic plant traits using image analysis fruit through processing. In 'THB' variety 'UENF/Caliman-01' hybrid images (A B) were analyzed estimate commercial irregularly shaped fruits. Image A was also used in estimation height, stem diameter first insertion height. fruits, largest smallest...
Complex biotherapeutic modalities, such as antibody-drug conjugates (ADC), present significant challenges for the comprehensive bioanalytical characterization of their pharmacokinetics (PK) and catabolism in both preclinical clinical settings. Thus, strategy ADCs must be designed to address specific structural elements protein scaffold, linker, warhead. A typical involves quantification Total ADC, IgG, Free Warhead concentrations. Herein, we PK a novel ADC. MEDI3726 targets prostate-specific...
Developing superior lines based on a simultaneous response from many traits of agronomic importance is fundamental strategy to expand papaya cultivar supply, mainly the much-needed cultivars belonging group Formosa. Our goal was associate pedigree method with REML/Blup procedure select plants progenies F2:3 performance. The variance components and genetic parameters were estimated using REML approach, values predicted Blup methodology. combined selection performed IG2 index weights...
Phenotyping to quantify the total carotenoids content (TCC) is sensitive, time-consuming, tedious, and costly. The development of high-throughput phenotyping tools essential for screening hundreds cassava genotypes in a short period time biofortification program. This study aimed (i) use digital images extract information on pulp color roots estimate correlations with TCC, (ii) select predictive models TCC using colorimetric indices. Red, green blue were captured root samples from 228...
AZD7442 (tixagevimab [AZD8895]/cilgavimab [AZD1061]) is a monoclonal antibody (mAb) combination in development for the prevention and treatment of coronavirus disease 2019. Traditionally, bioanalysis mAbs performed using ligand binding assays (LBAs), which offer sensitivity, robustness, ease implementation. However, LBAs frequently require generation critical reagents that typically take several months. Instead, we developed highly sensitive (5 ng/mL limit quantification) method hybrid...
The papaya crop occupies 32 thousand hectares of planted area in Brazil, with a total annual production 1.6 million tons (12.5% the world supply).The country stands out this scenario as second biggest producer fruit worldwide, only after India.The narrow genetic base once limited its variability, but use classical and molecular plant breeding techniques has enabled development number higher-yielding cultivars different levels resistance to fungal diseases.However, many studies still ought be...