A5082251944- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Treatment of Major Depression
- Nerve injury and regeneration
- 3D Printing in Biomedical Research
- Nicotinic Acetylcholine Receptors Study
- Memory and Neural Mechanisms
- Nitric Oxide and Endothelin Effects
- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Neurogenesis and neuroplasticity mechanisms
- Redox biology and oxidative stress
- Olfactory and Sensory Function Studies
- Biosensors and Analytical Detection
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neural Engineering
- Advanced biosensing and bioanalysis techniques
- Receptor Mechanisms and Signaling
- Cancer, Stress, Anesthesia, and Immune Response
- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Advanced Biosensing Techniques and Applications
- Regulation of Appetite and Obesity
- Axon Guidance and Neuronal Signaling
University of Verona
2015-2024
University of Milan
2017
University of Catania
1928
Clinical and epidemiological data show that biological sex is one of the major determinants for development progression cardiovascular disease (CVD). Impaired endothelial function, characterized by an imbalance in Nitric Oxide Synthase (eNOS) activity, precedes accelerates CVD. However, whether there any sexual dimorphism eNOS activity function cells (ECs) still unknown. Here, independently studying human male female ECs, we found ECs expressed higher mRNA protein levels both vitro ex vivo....
Brain and skull developments are tightly synchronized, allowing the cranial bones to dynamically adapt brain shape. At brain-skull interface, meninges produce trophic signals necessary for normal corticogenesis bone development. Meninges harbor different cell populations, including cells forming endosteum of vault. Recently, we other groups have described presence in a population endowed with neural differentiation potential vitro and, after transplantation, vivo. However, whether may be...
Clinical and experimental studies indicate that muscarinic acetylcholine receptors are potential pharmacological targets for the treatment of neurological diseases. Although these have been described in human, bovine rat cerebral microvascular tissue, a subtype functional characterization mouse brain endothelium is lacking. Here, we show all (M1-M5) expressed endothelial cells. The mRNA expression M2, M3, M5 correlates with their respective protein abundance, but mismatch exists M1 M4 versus...
The eventual role of blood pressure on the endothelial progenitor cell (EPC) has rarely been evaluated and data collected so far relate to patients with co-existing coronary heart disease.We have studied number functional activity EPC as well colony-forming units (CFU) in a carefully selected group 36 essential hypertension 24 normotensive control subjects.In hypertension, was not statistically different from that found subjects (mean +/- SD, 58 29, controls 53 20; EPC/high power field). CFU...
Mitochondrial diseases (MD) are heterogeneous disorders because of impairment respiratory chain function leading to oxidative stress. We hypothesized that in MD the vascular endothelium may be affected by increased oxidative/nitrative stress causing a reduction nitric oxide availability. therefore, investigated pathobiology vasculature patients assaying presence 3-nitrotyrosine muscle biopsies followed proteomic identification proteins which undergo tyrosine nitration. then measured...
Here we present a protocol to generate standardized cerebral organoids with hippocampal regional specification using morphogen WNT3a. We describe steps for isolating mouse embryonic (E14.5) neural stem cells from the brain subgranular zone, preparing samples immunofluorescence, calcium imaging, and metabolic profiling. This can be used developmental studies, modeling disease, drug screening. Organoids obtained in one month, thus providing rapid tool high-throughput data validation. For...
Gold nanostructures obtained by laser ablation in solution are functionalized with antibodies for two tumor-associated antigens (PSMA and PSCA) overexpressed prostate cancer cells incubated expressing one of the antigens, both or none. SERRS multiplexing measurements allow their identification show a great targeting specificity. Multiplexing allows simultaneous investigation different properties and, therefore, it will become an invaluable tool cell phenotyping in-vitro in-vivo tumour...
Brain organoids are in vitro three-dimensional (3D) self-organized neural structures, which can enable disease modeling and drug screening. However, their use for standardized large-scale screening studies is limited by high batch-to-batch variability, long differentiation time (10-20 weeks), production costs. This particularly relevant when brain obtained from human induced pluripotent stem cells (iPSCs). Here, we developed, the first time, a highly standardized, reproducible, fast (5...
Background and Purpose Methoxetamine (MXE) is a novel psychoactive substance that emerging on the Internet induces dissociative effects acute toxicity. Its pharmacological have not yet been adequately investigated. Experimental Approach We examined range of behavioural induced by administration MXE (0.5–5 mg·kg −1 ; i.p.) in rats whether it causes rapid neuroadaptive molecular changes. Key Results ) affected motor activity dose‐ time‐dependent manner, inducing hypermotility hypomotility at...
A bstract Tumor-associated macrophages (TAM) enhance cancer progression by promoting angiogenesis, extracellular matrix (ECM) remodeling, and immune suppression. Nerve infiltration is a hallmark of various cancers known to directly contribute tumor growth. However, the role TAM in intratumoral nerve growth remains poorly understood. In this study, we demonstrate that expressed distinct “neural growth” gene signature. actively neural within tumors promote neurites outgrowth. We identify...
Ketamine is a noncompetitive glutamate N-methyl-D-aspartic acid receptor antagonist. When acutely administered to rodents, it produces rapid antidepressant effect. There evidence that blockade enhances glutamatergic transmission preferentially engaging α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic receptors leading mTOR (mammalian target of rapamycin) pathways activation, thus resulting into downstream neuroadaptive changes in limbic structures. Recent in-vitro data on primary neuronal...
The expression of leptin and receptor (Ob-R) has been partially elucidated in colon patients with inflammatory bowel diseases (IBDs), even though is involved angiogenesis inflammation. We previously reported overexpression GLUT5 fructose transporter, aberrant clusters lymphatic vessels lamina propria IBD controls. Here, we examine Ob-R the same biopsies. Specimens were obtained from ulcerative colitis (UC), Crohn's disease (CD) controls who underwent screening for colorectal cancer,...
Although the effects of proximal smoking cues have been widely studied in smokers, little is known on features associated with background spatial context effect, that is, "context reactivity." The aim this study was to investigate reactivity exhibited by smokers virtual cue-free domestic scenarios.Sixty-nine participants divided 2 cohorts (33 and 36 non-smokers) were exposed a reality session 4 room scenarios presented balanced order: bedroom, bathroom, kitchen, living room.We showed (i) it...