A5032503290- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Birth, Development, and Health
- Energy and Environment Impacts
- RNA modifications and cancer
- Air Quality and Health Impacts
- Health, Environment, Cognitive Aging
- Pediatric health and respiratory diseases
- Privacy-Preserving Technologies in Data
- Heavy Metal Exposure and Toxicity
- Cancer-related gene regulation
- Cryptography and Data Security
- Blockchain Technology Applications and Security
- Intergenerational Family Dynamics and Caregiving
- Child Nutrition and Water Access
- Iron Metabolism and Disorders
- Genetic Associations and Epidemiology
- Gene expression and cancer classification
- Sleep and related disorders
- Childhood Cancer Survivors' Quality of Life
- Neonatal Respiratory Health Research
- Chronic Disease Management Strategies
- Gut microbiota and health
- Child Nutrition and Feeding Issues
- Cloud Computing and Resource Management
Menlo School
2024
Icahn School of Medicine at Mount Sinai
2018-2023
Technische Universität Berlin
2022
Sorbonne Paris Cité
2022
Université Paris Cité
2022
Heidelberg University
2015-2017
German Cancer Research Center
2015-2017
München Klinik
1988
Abstract DNA methylation (DNAm) has been revealed to play a role in various diseases. Here we performed epigenome-wide screening and validation identify mortality-related DNAm signatures general population-based cohort with up 14 years follow-up. In the discovery panel case-cohort approach, 11,063 CpGs reach genome-wide significance (FDR<0.05). 58 CpGs, mapping 38 well-known disease-related genes intergenic regions, are confirmed panel. A mortality risk score based on ten selected...
Mislabeled, contaminated or poorly performing samples can threaten power in methylation microarray analyses even result spurious associations. We describe a set of quality checks for the popular Illumina 450K and EPIC microarrays to identify problematic demonstrate their application publicly available datasets. Quality implemented here include 17 control metrics defined by manufacturer, sex check detect mislabeled sex-discordant samples, both an identity fingerprinting sample donors measure...
BackgroundDNA methylation (DNAm) may play a role in age-related outcomes. It is not yet known which DNAm-based biomarkers of age acceleration (BoAA) has the strongest association with endpoints.MethodsWe collected blood samples from two independent cohorts: Normative Ageing Study, and Cooperative Health Research Region Augsburg cohort. We measured epigenome-wide DNAm level, generated five BoAA at baseline. used Cox proportional hazards model to analyze relationships between all-cause death....
Saliva is a widely used biological sample, especially in pediatric research, containing heterogenous mixture of immune and epithelial cells. Associations exposure or disease with saliva DNA methylation can be influenced by cell-type proportions. Here, we developed reference panel to estimate interindividual heterogeneity whole studies. was collected from 22 children (7–16 years) sorted into cells, using size exclusion filtration magnetic bead sorting. measured the Illumina MethylationEPIC...
Colorectal cancer (CRC) is the third most common worldwide. If detected at an early stage, prognosis good. Despite increasing evidence for benefits of implemented screening programs, such as colonoscopy, compliance rather low. Hence there demand non-invasive tests detection CRC with high acceptance in population-wide screening. The objective this study was to identify and evaluate leukocyte DNA methylation patterns a potential biomarker CRC. Blood samples patients scheduled colonoscopy were...
DNA methylation microarrays have been the platform of choice for epigenome-wide association studies in epidemiology, but declining costs rendered targeted bisulphite sequencing a feasible alternative. Nonetheless, literature researchers seeking guidance on which to choose is sparse. To fill this gap, we conducted comparison study processed cord blood samples from four newborns duplicates using both Illumina HumanMethylationEPIC BeadChip and TruSeq Methyl Capture EPIC Kit, evaluated platforms...
Federated learning may be subject to both global aggregation attacks and distributed poisoning attacks. Blockchain technology along with incentive penalty mechanisms have been suggested counter these. In this paper, we explore verifiable off-chain computations using zero-knowledge proofs as an alternative in blockchain-based federated learning. our solution, nodes, addition their computational duties, act provers submitting attest correctness of param-eters that can verified on the...
Elderly individuals who are never smokers but have the same height and chronological age can substantial differences in lung function. The underlying biological mechanisms unclear. To evaluate associations of different biomarkers aging (BoA) function, we performed a repeated-measures analysis Normative Aging Study using linear mixed-effect models. We generated GrimAgeAccel, PhenoAgeAccel, extrinsic intrinsic epigenetic acceleration publically available online calculator. calculated Zhang's...
Several epigenome-wide association studies (EWAS) of ambient particulate matter with aerodynamic diameter ≤ 2.5 µm (PM2.5) have been reported. However, EWAS PM2.5 elements (PEs), reflecting different emission sources, are very limited. We performed short- and intermediate-term exposure to 13 PEs. hypothesized that significant changes in DNAm may vary by mass its elements. repeatedly collected blood samples the Normative Aging Study measured leukocyte DNA methylation (DNAm) Illumina...
Abstract The effects of prenatal lead exposure on child development include impaired growth and cognitive function. DNA methylation might be involved in the underlying mechanisms previous epigenome-wide association studies reported associations between during pregnancy cord blood levels. However, it is unclear which developmental stage most harmful. Cord levels were assayed 420 children from a Mexican pre-birth cohort using Illumina Infinium MethylationEPIC microarray. Lead concentrations...
Aim: Whole-blood DNA methylation depends on the underlying leukocyte composition and confounding hereby is a major concern in epigenome-wide association studies. Cell counts are often missing or may not be feasible. Computational approaches estimate from based reference datasets of purified leukocytes. We explored possibility to train such model whole-blood cell without need for purification. Materials & methods: Using corresponding five-part 2445 participants London Life Sciences...
The Illumina Infinium HumanMethylation450 BeadChip is frequently used in epigenetic research. Besides quantile normalization there currently no standard method to normalize the data between arrays. We describe some properties of generated by this platform and present a based on local regression. compare performance with other commonly approaches three benchmarks (correlation 21 pairs technical replicates, detection differential methylation correlation levels for smoking-associated CpG sites...
One concern in epigenome-wide studies investigating leukocyte DNA methylation is that observed associations may at least partly reflect differences composition (LC) rather than changes methylation. We estimated the magnitude of confounding by LC for common risk factors and diseases.Variation according to sex, race, age, smoking, alcohol consumption, BMI, cardiovascular fitness, hypertension, coronary heart disease diabetes was analyzed using blood differentials from 4117 participants NHANES....
Epigenome-wide association studies of ambient fine particulate matter (PM2.5) have been reported. However, few examined PM2.5 components (PMCs) and sources or included repeated measures. The lack high-resolution exposure measurements is the key limitation. We hypothesized that significant changes in DNA methylation might vary by PMCs sources.We predicted annual average 14 using novel models across contiguous U.S., between 2000-2018. resolution was 50 m × Greater Boston Area. also identified...
Both parental and neighbourhood socio-economic status (SES) are linked to poorer health independently of personal SES measures, but the biological mechanisms unclear. Our objective was examine these influences via epigenetic age acceleration (EAA)-the discrepancy between chronological ages.We examined three USA-based [Coronary Artery Risk Disease in Adults (CARDIA) study, Fragile Families Child Wellbeing Study (FFCWS) Programming Research Obesity, Growth, Environment Social Stressors...
Long‐lasting oxidative stress exposure may lead to relatively stable epigenetic modifications of the DNA in order activate anti‐oxidative defence mechanisms. Oxidative related methylation therefore be associated (causally or as a by‐product) with cancer. We measured derivatives reactive oxygen metabolites (D‐ROM), total thiol levels (TTL) and Illumina Infinium 450K BeadChip three samples German individuals aged ≥50 years: n = 1,000 ESTHER study baseline participants (DNA only), 99 eight‐year...
Altered maternal haemoglobin levels during pregnancy are associated with pre-clinical and clinical conditions affecting the fetus. Evidence from animal models suggests that these associations may be partially explained by differential DNA methylation in newborn possible long-term consequences. To test this humans, we meta-analyzed epigenome-wide of offspring 3,967 cord blood 1,534 children 1,962 adolescent whole-blood samples derived 10 cohorts. was measured using Illumina Infinium...
Abstract Introduction: Identifying cancer subtypes is necessary for diagnosis, prognosis determination, and treatment selection. Furthermore, as transformation between increasingly recognized a key resistance mechanism to targeted therapies, serial subtype reassessment likely gain adoption. Cancer have traditionally been determined by pathologists based on histology; more recently, molecular subtyping has performed using IHC, RNAseq, or assays that detect genetic alterations. However, these...
OPS 20: Chemicals, biomarkers, omics, Room 110, Floor 1, August 27, 2019, 4:30 PM - 5:30 Background: Phthalates, ubiquitous chemicals used in many household and personal care products, act as endocrine disruptors interfering with normal child development. DNA methylation may be a mechanism involved previous studies have linked prenatal exposure to altered cord blood, but independent validation is necessary. Methods: We conducted hypothesis-free epigenome-wide discovery of associations...