A5009607993- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Hepatitis Viruses Studies and Epidemiology
- Gut microbiota and health
- Antifungal resistance and susceptibility
- Probiotics and Fermented Foods
- Enterobacteriaceae and Cronobacter Research
- Fungal Infections and Studies
- Nutrition and Health in Aging
- Immune Response and Inflammation
- Clostridium difficile and Clostridium perfringens research
- Animal Disease Management and Epidemiology
- Protein Hydrolysis and Bioactive Peptides
- Clinical Nutrition and Gastroenterology
- Respiratory and Cough-Related Research
- Plant Pathogens and Fungal Diseases
- Virus-based gene therapy research
- Advanced Thermodynamic Systems and Engines
- Infection Control and Ventilation
- Barrier Structure and Function Studies
- Water-Energy-Food Nexus Studies
- Wastewater Treatment and Nitrogen Removal
- Parasitic Diseases Research and Treatment
- Fungal and yeast genetics research
Université de Bourgogne
2010-2023
Candid
2023
Pathophysiology, Diagnosis and Treatment of Bone Diseases
2023
Institut Agro Dijon
2019-2022
Université Bourgogne Franche-Comté
2016-2020
Procédés Alimentaires et Microbiologiques
2019-2020
Agroécologie
2012-2016
CHU Dijon Bourgogne
2002-2014
Infection et inflammation
2010-2011
Maison des Sciences de l’Homme de Dijon
2008
ABSTRACT An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects 13 smaller clusters France was analyzed using epidemiological clinical data shellfish samples. Environmental information from the oyster-producing area, located lagoon southern France, collected investigate possible events leading contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) same primer sets for both environmental After...
ABSTRACT Group A rotaviruses, human caliciviruses, astroviruses, and adenovirus types 40 41 were detected by enzyme immunoassay or reverse transcription-PCR in 61, 14, 6, 3% of stool specimens from 414 children consulting for gastroenteritis between 1995 1998. These data highlight the importance caliciviruses infantile gastroenteritis. Among these, Norwalk-like viruses belonging to genogroup II predominant.
ABSTRACT Aichi virus has been proposed as a causative agent of gastroenteritis. A total 457 stool specimens from children hospitalized with acute diarrhea and 566 adults involved in 110 gastroenteritis outbreaks were screened for the presence by reverse transcription-PCR (RT-PCR) amplification genomic region 3C 3D (3CD) nonstructural proteins. Our results show low incidence pediatric samples existence mixed infections other microbiological agents some cases. From outbreak survey, it appears...
ABSTRACT We compiled sequence and epidemiological data from 172 caliciviruses detected in France December 1998 to February 2004 sporadic outbreak cases. The results showed a cocirculation of strains with majority genogroup II (GII) noroviruses. Three groups noroviruses, not before our laboratory, emerged spread during the period: recombinant GGIIb Norwalk-related amplified polymerase gene 2000 new Lordsdale variant 2002. observed that (i) GII-4 noroviruses were predominant nursing home...
In France, an epidemic peak of acute diarrhea is observed each winter. Previous results suggested a viral etiology for these winter epidemics. We investigated the role enteric viruses in and their molecular diversity. One hundred sixty-one patients with 45 healthy (controls) from general population were given standardized questionnaire between December 1998 May 1999. Stool specimens screened group A C rotaviruses, human caliciviruses, astroviruses, adenovirus types 40 41 by reverse...
Noroviruses are important etiologic agents of acute gastroenteritis and show great genetic diversity. To characterize more fully previously detected strains that could not be assigned unequivocally to one particular genotype based on the RNA polymerase, we have sequenced a region in capsid gene and, some cases, junction between open reading frame 1 (ORF 1) ORF 2. The results allowed us identify several recombinant noroviruses: GGIIb viruses were for first time France August 2000 then spread...
In anticipation of vaccine introduction, we assessed epidemiology rotavirus disease among children visiting medical centre due to acute diarrhoea in Ouagadougou, Burkina Faso. Between November 2008 and February 2010, stool specimens from 447 less than 5 years age suffering were tested for the presence by antigen detection using an immunochromatographic test. Sociodemographic, environmental clinical factors during study. Rotavirus was detected 151 (33.8%) patients. Most cases (94.2%) < 24...
C. albicans is a commensal yeast of the mucous membranes in healthy humans that can also cause disseminated candidiasis, mainly originating from digestive tract, vulnerable patients. It necessary to understand cellular and molecular mechanisms interaction with enterocytes better basis commensalism pathogenicity improve management candidiasis. In this study, we investigated kinetics tight junction (TJ) formation parallel invasion into Caco-2 intestinal cell line. Using invasiveness assays on...
ABSTRACT Rotavirus G and P types from 716 children with acute diarrhea in Dijon 1995 to 1998 throughout France during the winter of 1997-1998 were analyzed by reverse transcription-PCR. P[8],G1 predominated, followed P[8],G4, which emerged last winter. G9 P[6] strains detected at low frequencies.
Candida albicans (C. albicans) is an opportunistic pathogen causing infections ranging from superficial to life-threatening disseminated infections. In a susceptible host, C. able translocate through the gut barrier, promoting its dissemination into deeper organs. hyphae can invade human epithelial cells by two well-documented mechanisms: epithelial-driven endocytosis and albicans-driven active penetration. One mechanism which host protect themselves against intracellular termed autophagy....
In this study, the diversity of G and P genotypes rotavirus strains in Burkinabe children were examined. Between November 2008 February 2010, 447 stool samples collected from <5 years age with acute diarrhea visiting hospital Ouagadougou. Group A was previously detected 151/447 (33.8%) tested by an immunochromatographic test these now further for RT-PCR. Of these, type genes amplified RT-PCR 140/151 (92.7%) successfully determined 81 (57.9%) 130 (92.9%) samples, respectively. The most...
ABSTRACT In September 2001, a waterborne outbreak of gastroenteritis occurred in eastern France. Of 31 fecal samples from symptomatic individuals, 19 tested positive for Cryptosporidium with two PCRs targeting the Hsp70 and 18S rRNA genes . Sequencing PCR fragments produced sequences identical to that parvum genotype 1.
Pathogenic mycobacteria such as M. tuberculosis, bovis or leprae are characterised by their extremely slow growth rate which plays an important role in mycobacterial virulence and eradication of the bacteria. Various limiting factors influence generation time mycobacteria, DNA-binding protein 1 (MDP1) has also been implicated regulation. Our strategy to investigate MDP1 consisted characterisation a BCG derivative expressing MDP1-antisense gene. The expression recombinant containing plasmid...
Secretory IgA interacts with commensal bacteria, but its impact on human mycobiota ecology has not been widely explored. In particular, whether IgA-deficiency is associated gut fungal dysbiosis remains unknown. Our goal was to study the of ecology. The Fungi-Flow method used characterize fecal, systemic, and maternal IgA, IgM, IgG responses against 14 representative strains (yeast/spores or hyphae forms) in healthy donors (HDs) (n = 34, 31, 20, respectively) also compare opsonization by...
Abstract Transcription factor Rme1 is conserved among ascomycetes and regulates meiosis pseudohyphal growth in Saccharomyces cerevisiae . The genome of the meiosis-defective pathogen Candida albicans encodes an homolog that part a transcriptional circuitry controlling hyphal growth. Here, we use chromatin immunoprecipitation genome-wide expression analyses to study possible role C. morphogenesis. We find binds upstream activates genes are upregulated during chlamydosporulation, asexual...
ABSTRACT This work describes the design and initial evaluation of a commercial test allowing detection noroviruses sapoviruses by reverse transcription-PCR (RT-PCR) in single tube followed microplate hybridization, as well PCR inhibitors. The was shown to be broadly reactive (except for Melksham-like strains), sensitive, specific thus should useful calicivirus clinical practice.
LT-R192G, a mutant of the thermolabile enterotoxin E. coli, is potent adjuvant immunization. Immune responses are generally analyzed at end protocols including least 2 administrations, but rarely after prime. To investigate this point, we compared B and T cell in mice one two intrarectal immunizations with 2/6 rotavirus-like particles (2/6-VLP) LT-R192G. After boost, found, an unexpected lower expansion measured by flow cytometry, despite secondary antibody response. We then CD4+CD25+Foxp3+...