A5052765696- Nitric Oxide and Endothelin Effects
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Sphingolipid Metabolism and Signaling
- Peroxisome Proliferator-Activated Receptors
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Neurological Disease Mechanisms and Treatments
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Eicosanoids and Hypertension Pharmacology
- Diabetes and associated disorders
- Gut microbiota and health
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Lipid metabolism and disorders
- Cytokine Signaling Pathways and Interactions
- Adipose Tissue and Metabolism
- Natural Compound Pharmacology Studies
- Traditional Chinese Medicine Analysis
- Diabetes Treatment and Management
- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Neurological Disorders and Treatments
- Kruppel-like factors research
Central South University
2008-2022
Xiangya Hospital Central South University
2007-2022
Nonalcoholic fatty liver disease (NAFLD) is one of the most common metabolic disorder diseases, which include a histological spectrum conditions ranging from simple steatosis to nonalcoholic steatohepatitis (NASH). Dysregulated metabolism sphingomyelin in plays critical role pathogenesis NAFLD. Ceramides are central molecules sphingolipid biosynthesis and catabolism play an important insulin resistance, apoptosis, inflammation. In addition, apoptosis main contributor development This study...
Previous in vitro and vivo studies have demonstrated that vitamin D could prevent pancreatic beta-cell destruction reduce the incidence of autoimmune diabetes. In children with type 1 diabetes, treatment produces moderate protective effects on residual function has proven to be safe. Therefore, we hypothesized might patients latent diabetes adults (LADA), a form slowly progressive diabetes.Thirty-five LADA were randomly assigned receive subcutaneous insulin alone (n = 18) or plus...
High bone mass (HBM) is usually caused by gene mutations, and its mechanism remains unclear. In the present study, we identified a novel mutation in long noncoding RNA Reg1cp that associated with HBM. Subsequent analysis 1,465 Chinese subjects revealed heterozygous individuals had higher density compared WT Reg1cp. Mutant increased formation of CD31hiEmcnhi endothelium marrow, which stimulated angiogenesis during osteogenesis. Mechanistically, mutant directly binds to Krüppel-like factor 3...
Abstract Cell therapy with bone marrow stem cells ( BMSC s) remains a viable option for tissue repair and regeneration. A major challenge cell is the limited survival after implantation. This study was to investigate effect of oxidized low‐density lipoprotein (ox‐ LDL , naturally present in human blood) on injury MG 53, protein, improvement survival. Rat multipotent adult progenitor MAPC were treated ox‐ which caused significant death as reflected by increased LDH release media. Exposure s...
Diabetic vascular complications result from high-glucose induced endothelial cell dysfunction. There is an emerging need for novel drugs with protective effects the treatment of diabetic complications. The present study aimed to investigate effect α-mangostin against apoptosis cultured human umbilical vein cells (HUVECs). HUVECs were treated glucose induce apoptosis. expression apoptosis-related proteins, Bcl-2, Bax, and cleaved caspase-3, detected by Western blotting. Ceramide concentration...
Abstract Acid sphingomyelinase (aSMase) plays an important role in endothelial dysfunction. Here, we show that elevated aSMase activity and ceramide content were reduced by desipramine treatment diabetic animals. The inhibitor of aSMase, desipramine, improved vascular dysfunction db/db mice. High glucose (HG)-induced up-regulation levels restored with siRNA or cells. In addition, increased the release nitric oxide (NO) phosphorylation NO synthase (eNOS) mouse aortas aortic cells HG. These...
This study was to investigate the effect of high glucose (HG) on vascular endothelial growth factor (VEGF) expression in bone marrow stem cells and JAK2/STAT-3 signalling. Adult rat multipotent progenitor (rMAPCs) were cultured evaluate VEGF (both mRNA protein) with or without exposure HG for up 48 hrs using RT-PCR ELISA. JAK2 STAT3 phosphorylation rMAPCs analysed by Western blotting. With normal media, level after 24 culture significantly increased 15 times over baseline (day 0) detectable...
Abstract Diabetic vascular complications are the leading causes of death and disability in patients with diabetes. Alpha‐mangostin has been reported to have anti‐diabetic capacity recent years. Here, we investigated protective function alpha‐mangostin on endothelium vitro vivo experiments. We also observed that improved impaired endothelium‐dependent vasodilation (EDV) diabetic animals while it limited aSMase/ceramide pathway up‐regulated eNOS/NO aortas from mice. Meanwhile, inhibited...
Previous studies have shown that the phosphatidylinositol 3-kinase / Akt endothelial nitric oxide synthase NO (PI3K/Akt/eNOS/NO) pathway is involved in high glucose-induced cell apoptosis and rosiglitazone has a protective effect on endothelium. In present study, we investigated antiapoptotic of human umbilical vein cells (HUVECs) exposed to glucose explored its possible mechanism. Treatment (33 mmol/L) for 48 h significantly induced HUVECs, concomitantly with increased caspase-3 activity....
To investigate how ceramide mediates the effects of high-glucose-induced inhibition Akt/endothelial nitric oxide synthase (eNOS) signalling pathway in human vascular endothelial cells (HUVECs).NO levels were determined by ELISA. Endogenous using a liquid chromatography-mass spectrometry assay. Akt and eNOS protein expressions Western blotting.High-glucose induce accumulation dose- time-dependent manner (p<0.05). We also show that exposure HUVECs to high-glucose conditions inhibits...
1. The transcription factor Oct4 is critical to the pluripotency, self-renewal and differentiation of stem cells. aim present study was investigate effects high glucose (HG) on cell cycle progression bone marrow multipotent adult progenitor cells (MAPC) expression, as well underlying mechanisms. 2. Rat MAPC were cultured in normal (5.5 mmol/L D-glucose) HG (25.5 media for up 14 days. L-Glucose served a osmolarity control. Culture substantially increased number G(0)/G(1) phase decreased S...
To compare the effect of intermittent and constant high glucose media on synthesis vascular relaxing factor nitric oxide (NO) in cultured human umbilical vein endothelial cells (HUVECs), to investigate its mechanism.HUVECs were exposed 5.5 mmol/L (normal control), 20 (constant glucose), alternating with (intermittent glucose) for 7 days. The NO level was measured using Griess Reaction cell culture supernatants; expressions PI3K, PKB eNOS mRNA protein RT-PCR or Western blot.NO group...
Papillary thyroid carcinoma (PTC) is the most common type of cancer (TC). Considering important association between cellular immunity and PTC progression, it worth exploring biological significance immune-related signaling in PTC. Several bioinformatics tools, such as R software, WEB-based Gene SeT AnaLysis Toolkit (WebGestalt), Database for Annotation, Visualization Integrated Discovery (DAVID), Search Tool Retrieval Interacting Genes (STRING) Cytoscape were used to identify hub genes...
This study intended to determine the associations between gut microbiota and glucose response in healthy individuals analyze connection microbiome glucose-metabolism-related parameters.Fecal bacterial composition anthropometric, body composition, fat distribution, biochemical measures were analyzed. A 75-g oral tolerance test (OGTT) was given each participant investigate changes glucagon-like peptide 1 (GLP-1), insulin, glucose. The whole regions of interest local analyzed using dual-energy...