A5001003322- Neonatal and Maternal Infections
- Bacterial Infections and Vaccines
- Neonatal and fetal brain pathology
- Preterm Birth and Chorioamnionitis
- Pregnancy and preeclampsia studies
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Reproductive System and Pregnancy
- Genetic factors in colorectal cancer
- ATP Synthase and ATPases Research
- Angiogenesis and VEGF in Cancer
- Endometriosis Research and Treatment
- CAR-T cell therapy research
- Ferroptosis and cancer prognosis
- Melanoma and MAPK Pathways
- COVID-19 Impact on Reproduction
- interferon and immune responses
- earthquake and tectonic studies
- Renal cell carcinoma treatment
- Cytomegalovirus and herpesvirus research
- Reproductive tract infections research
- Cytokine Signaling Pathways and Interactions
- Organoselenium and organotellurium chemistry
- Renal and related cancers
- Protein Tyrosine Phosphatases
National Health and Family Planning Commission
2024
Fuzhou Maternity and Child Health Care Hospital
2022-2024
Fujian Medical University
2022-2024
Fujian Normal University
2018-2020
Hefei University
1986
Carbon dots (CDs) have been utilized to form Schiff bases. The prepared CD-based bases exhibit significant anticancer activity in glioma cells.
Matrix metallopeptidase 9 (MMP9) was found to be associated with tumor aggressiveness. In this study, we focused on the correlation between MMP9 expression and clear cell renal carcinoma (ccRCC). Through Gene Expression Omnibus (GEO) database, Cancer Genome Atlas (TCGA) database immunohistochemical (IHC) staining, observed that compared adjacent normal tissues, in ccRCC tissues mRNA protein levels of were enhanced, GTP-binding smg p21B(RAP1B), B rapidly accelerated fibrosarcoma (RAF), methyl...
Abstract Background Streptococcus agalactiae or group B (GBS) is a leading infectious cause of neonatal morbidity and mortality. It essential to establish robust method for the rapid ultra-sensitive detection GBS in pregnant women with premature rupture membrane (PROM). Methods This study developed CRISPR-GBS assay that combined advantages recombinase polymerase amplification (RPA) CRISPR/Cas12a system detection. The clinical performance was assessed using vaginal cervical swabs were...
Abstract Congenital heart disease (CHD) is the most common type of birth defect and main noninfectious cause death during neonatal stage. The non-POU domain containing, octamer-binding gene, NONO , performs a variety roles involved in DNA repair, RNA synthesis, transcriptional post-transcriptional regulation. Currently, hemizygous loss-of-function mutation have been described as genetic origin CHD. However, essential effects cardiac development not fully elucidated. In this study, we aim to...
Cutaneous melanoma (CM) is an aggressive form of skin cancer with limited treatment options for advanced stages. Prognostic markers that accurately predict patients' outcomes and guide therapeutic strategies are crucial improving management. SETD2 (SET Domain-Containing Protein 2), a histone methyltransferase involved in chromatin remodeling gene regulation, has recently emerged as tumor suppressor. Its dysfunction oncogenesis some cancers, but little known about its functions progression...
Breast Cancer (BRCA) is one of the most common cancers worldwide. Abnormal Alternative Splicing (AS) frequently observed in cancers. Understanding intricate relationship between gene mutations and abnormal AS vital for developing novel diagnostic therapeutic strategies to effectively target cancer. This study aimed focus on analysis transcriptomic splicing events patients with (BRCA), particularly those TP53 gene. role may be helpful revealing potential predictive indicators survival...
Endometrial decidualization is a decidual tissue formed by the proliferation and re-differentiation of endometrial stroma stimulated inducing factors. It very important for proper maintenance pregnancy. Previous studies speculated that Golgi phosphoprotein 3 (GOLPH3) may have regulatory role in process decidualization, while specific molecular mechanisms GOLPH3 unclear. In this part, was silenced human stromal cells (hESCs), transcriptome data (RNA-seq) knockdown (siGOLPH3) obtained...
ABSTRACT Upon HSV-1 infection, viral protein 16 (VP16), supported by Host Cell Factor C1 (HCFC1), is rapidly transported into the nucleus, and help to express a series of immediate-early proteins begin its lytic replication. However, no direct evidence has shown if HCFC1 deficiency can affect proliferation so far. Here, we showed that led strong resistance infection. Moreover, identified Regulator 1 (HCFC1R1) as new host factor acting early in HSV infection for transport capsid nucleus. The...
近年来，利用重组病毒对T细胞进行基因编辑用于免疫治疗，受到了广泛重视。然而，重组病毒因存在随机整合，制备耗时长且昂贵的缺点制约了其应用。与此同时，电转染技术的应用能够快速将外源DNA带入细胞内，有助于提高T细胞基因编辑效率。TET（Ten-eleven translocation）家族蛋白可以催化5-甲基胞嘧啶（5mC）转化为5-羟甲基胞嘧啶（5hmC），5hmC作为细胞中的DNA去甲基化酶，在细胞基因组表观遗传学中起着重要调控作用。研究表明TET2基因的缺失能够促进CAR-T细胞的快速繁殖，产生强力的CAR-T细胞。该研究利用CRISPR/Cas9基因编辑技术对TET2基因进行敲除。首先对sgRNA进行体外转录，与大肠杆菌诱导表达的Cas9蛋白孵育形成Cas9：gRNA核糖核蛋白复合物（RNP），并在体外酶切验证sgRNA的活性。接着利用电转染技术将Cas9：gRNA核糖核蛋白复合物（RNP）带入细胞内，并检测T细胞基因编辑效率。最后利用流式细胞分析技术检测T细胞的增殖情况。基因测序与T7E...
Abstract Background Streptococcus agalactiae or group B (GBS) is a leading infectious cause of neonatal morbidity and mortality. It essential to establish robust method for the rapid ultra-sensitive detection GBS in pregnant women with premature rupture membrane (PROM). Methods This study developed CRISPR-GBS assay that combined advantages recombinase polymerase amplification (RPA) CRISPR/Cas12a system detection. The clinical performance was assessed using vaginal cervical swabs were...