Abstract Missense variants that change the amino acid sequences of proteins cause one-third human genetic diseases 1 . Tens millions missense exist in current population, and vast majority these have unknown functional consequences. Here we present a large-scale experimental analysis across many different proteins. Using DNA synthesis cellular selection experiments quantify effect more than 500,000 on abundance 500 protein domains. This dataset reveals 60% pathogenic reduce stability. The...

Abstract Missense variants that change the amino acid sequences of proteins cause one third human genetic diseases 1 . Tens millions missense exist in current population, with vast majority having unknown functional consequences. Here we present first large-scale experimental analysis across many different proteins. Using DNA synthesis and cellular selection experiments quantify impact >500,000 on abundance >500 protein domains. This dataset, Human Domainome 1, reveals >60%...

Currently, CD133 is one of the best markers to characterize cancer stem cells and Her‐1 reported as an important marker for prognosis triple‐negative breast cancer. To investigate relationship between expression clinicopathology well in cancer, 67 cases invasive ductal carcinoma taken from 422 patients with were analyzed by immunohistochemistry follow‐up. The expressed positive 43.3% (29/67) 53.7% (36/67) patients, respectively. corresponded tumor size ( P = 0.022), clinical stage 0.001)...

In our study, Bacillus subtilis was disposed to a simulated microgravity (SMG) environment in high-aspect ratio rotating-wall vessel bioreactors for 14 days, while the control group same normal gravity (NG) days. The B. strain exposed SMG (labeled BSS) showed an enhanced growth ability, increased biofilm formation sensitivity ampicillin sulbactam and cefotaxime, some metabolic alterations compared with under NG conditions BSN) original of BSO). differentially expressed proteins (DEPs)...

Many studies have shown that the space environment plays a pivotal role in changing characteristics of conditional pathogens, especially their pathogenicity and virulence. However, Stenotrophomonas maltophilia , type pathogen has to gradual increase clinical morbidity recent years, rarely been reported for its impact space. In this study, S. was exposed simulated microgravity (SMG) high-aspect ratio rotating-wall vessel bioreactors 14days, while control group same normal gravity (NG)...

Multidrug resistance is a growing global public health threat with far more serious consequences than generally anticipated. In this study, we investigated the antibiotic and genomic traits of clinical strain Escherichia coli LCT-EC001. LCT-EC001 was resistant to 16 kinds widely used antibiotics, including fourth-generation cephalosporins carbapenems. total, up 68 determinants associated were identified, 8 beta-lactamase genes (notably producing ESBLs KPCs), 31 multidrug efflux system genes,...

Escherichia coli is a Gram-negative, rod-shaped bacterium that commonly found in the intestine of warm-blooded organisms. Most E. strains are harmless, but some serotypes can cause serious food poisoning humans. Here, we present complete genome sequence LCT-EC106, which was isolated from CGMCC 1.2385.

Microbes threaten human health in space exploration. Studies have shown that Proteus mirabilis has been found habitats. In addition, the biological characteristics of P. studied unconditionally. The simulated microgravity environment provides a platform for understanding changes mirabilis.This study intends to explore effect on mirabilis, formation biofilm, and its related mechanism.The strange deformable rods were cultured continuously 14 days under high-aspect rotating vessels (HARVs)....

Papillary renal cell carcinoma (PRCC) is a common carcinoma. Recent studies have reported that ferroptosis involved in the occurrence and development of tumors. Long non-coding RNAs can be used as independent biomarkers for diagnosis prognosis variety tumors.Gene expression profile clinical information patients with PRCC were obtained from The Cancer Genome Atlas (TCGA) database. Lasso penalized Cox regression univariate analysis utilized model construction. Kaplan-Meier (K-M) receiver...

Bacillus cereus strain LCT-BC25, which was carried by the Shenzhou VIII spacecraft, traveled in space for about 398 h. To investigate response of B. to environments, we determined genome sequence isolated after flight.

In order to explore the effect of space environments on Bacillus cereus, we determined draft genome sequence a B. cereus strain, LCT-BC235, which was isolated after flight.

Abstract Space flight missions last for a long time so bacterial infection during is considered potential risk astronauts. Studies of antibiotic resistance under spaceflight and simulated microgravity (SMG) have shown lots results. To better understand the Klebsiella pneumoniae in environment, an original K. strain was cultured SMG conditions combined with background exposure (SMGA) as experimental strain, while control normal gravity environment without exposure. At beginning treatment,...

The transition in Pakistani cinema and terming it as a rival of heavily depends on the bowing technique item songs, which is deliberately fueling negative representation females. qualitative analysis songs underlines women sheds light aspects regarding how objectification done patriarchal society what values are assigned based gender. revealed that were sexually objectified use clothing, jewelery, makeup, body movements, gestures filmed to enhance their bodies. Hence, most used females an...

ABSTRACT The space environment has been shown to affect microbes by altering various features, including morphology, growth rate, metabolism, virulence, drug resistance, and gene expression mutation. Here we present the draft genome sequence of Enterococcus faecium strain LCT-EF258, derived from E. CGMCC 1.1736, which was exposed 17-day flight.

Abstract Background: Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) have been widely used as first-line therapy for patients with EGFR mutant non-small cell lung cancer (NSCLC). However, EGFR-TKIs treatment of NSCLC will inevitably produce acquired drug resistance. It greatly limits the therapeutic effect TKI. Recent studies found that some natural drugs combined TKI in may not only improve efficacy but also reduce occurrence This study intends to investigate...

Serratia marcescens has been detected in space habitats. To explore the influence of flight environment on this bacterium, we investigated genome sequence LCT-SM166, which was isolated after and a specific carbon source utilization pattern.

To evaluate the feasibility, safety and clinical efficacy of percutaneous microwave ablation (MVA) uterine leiomyoma. 13 leiomyoma in 10 patients were ablated by (MV). 11 tumors punctured under direct vision during operation 2 percutaneously US-guidance. Biopsy was performed before MVA. The tension tumor capsule increased progressively looked pale with prolongation MV emmission. After MVA, shrunk gradually became crimped. serosa at puncture site undamaged 9 burned due to short insertion...

To investigate the clinicopathological significance of c-kit protein expression in small cell lung cancer (SCLC).c-kit was detected 52 formalin-fixed and paraffin-embedded SCLC, 20 non-small (NSCLC) 10 normal tissues by immunohistochemistry with rabbit antibody to human protein.c-kit expressed 61.5% (32/52) primary but negative all samples NSCLC tissues. localized cytoplasm cells. The related tumor size ( P =0.010) prognosis =0.000 1), not sex =0.208), age =1.000) or TNM stage =0.722)...

Abstract Background Microbes threaten human health in space exploration. Studies have shown that P. mirabilis has been found habitats. In addition, the biological characteristics of studied unconditionally. The simulated microgravity environment provides a platform for understanding changes . Objective This study intends to explore effect on , formation biofilm and its related mechanism. Methods strange deformable rods were cultured continuously 14 days under by (HARVs) high-aspect ratio...