Abstract Background Highly malignant high-grade gliomas are tumors of the central nervous system (CNS). They solid arising from transformed cells brain and/or spinal cord. In recent years, expression genes and regulating miRNAs in glial has been actively studied. The present study is devoted to assessing levels miR-215-5p, miR-22-3p, miR-122-5p, miR-107, miR-324-5p, miR-34a-5p, miR-155. -5p, miR-21-5p, miR-497-5p, miR-330-3p, miR-146a-5p, miR-92a-1-5p, miR-326 target EGFR, SMAD4, SMAD7, SMO,...

УРОВЕНЬ ОПУХОЛЕВЫХ И ВНЕКЛЕТОЧНЫХ МИКРОРНК У ПАЦИЕНТОВ С ГЛИАЛЬНЫМИ ОПУХОЛЯМИ ГОЛОВНОГО МОЗГАПушкин А.А. 1,2, Гвалдин Д.Ю. 1 , Петрусенко Н.А. Росторгуев Е

Low toxicity, oncospecificity, immunogenicity and relatively frequent expression of CT antigens in many types cancer justify their use immunotherapy. This review presents the data on CTA new clinical approaches to malignant process control, developed from ideas anti-tumor immunity mechanisms. In particular, we highlight results application immune checkpoint inhibitors, external correction response by vaccination with ex vivo generated dendritic cells, natural killer cells T-lymphocytes;...

Abstract Background The purpose of this study was to characterize subtype-specific patterns mRNA and miRNA expression gliomas using Cancer Genome Atlas (TCGA) data search for genetic determinants that predict prognosis in terms overall survival create interaction networks grade 2 3 (G2 G3) astrocytomas, oligodendrogliomas 4 (G4) glioblastoma multiforme. Based on open-access TCGA data, 5 groups were formed: astrocytoma G2 ( n = 58), G3 128), oligodendroglioma 102), 72) G4 564); normal samples...

Федеральное государственное бюджетное учреждение «Ростовский научно-исследовательский онкологический институт» Министерства здравоохранения

Purpose of the study. This work is devoted to study blood plasma miRNA patterns in using high-throughput sequencing Omnibus Gene Expression base and search for candidate molecules development a minimally invasive diagnostic panel. Materials methods. Basing on open dataset NCBI GSE150956 Gene, groups samples with glioblastoma conventionally healthy donors were formed. For each sample, information levels expression was extracted. Determination significant miRNAs machine learning algorithms R...

ОЦЕНКА ДИАГНОСТИЧЕСКОЙ ЗНАЧИМОСТИ ЦИРКУЛИРУЮЩИХ МИКРОРНК В ПЛАЗМЕ КРОВИ ПАЦИЕНТОВ С ГЛИОМАМИ ВЫСОКОЙ СТЕПЕНИ ЗЛОКАЧЕСТВЕННОСТИАллилуев И.А. 1,2, Пушкин А.А. 1 , Кузнецова Н

Aim: to study the effect of a pulsed magnetic field (PMF) on expression key tumor suppressor genes, such as aPc, MLH, and MGMt in human t98G glioblastoma cell line. material methods . PMF with intensity 15 300 mt was used alone combination ionizing radiation at single dose 10 Gy. perform radiation, theratron Equinox 60 co unit Best theratronics Ltd., Ottawa, canada) used. source Neuro-Ms / D therapeutic advanced device Neurosoft company. Live dead cells were determined NanoEntekJuliFl...

Purpose of the study . This research was devoted to mRNA and miRNA expression patterns in glioglastomas using The Cancer Genome Atlas (TCGA) data, search for genetic determinants that determine prognosis patient survival create interaction networks glioblastomas. Materials methods. Based on data open TCGA database groups glioblastomas conventionally normal brain tissue samples were formed. Survival gene extracted each sample. After stratification by differential analysis genes affecting...

ГЕНДЕРНЫЕ ОСОБЕННОСТИ ЭКСПРЕССИИ ГЕНЕТИЧЕСКИХ ЛОКУСОВ В ТКАНЯХ ГЛИОМ Кит О.И. 1 , Пушкин А.А. Росторгуев Э.Е. Поркшеян Д.Х. Франциянц Е.М. Кузнецова Н.С. Черкиев И

ИЗМЕНЕНИЕ ЭКСПРЕССИОННОГО СТАТУСА ГЕНОВ ПРИ МАЛИГНИЗАЦИИ КЛЕТОК МОЗГА Кит О.И. 1 , Пушкин А.А. Росторгуев Э.Е. Поркшеян Д.Х. Франциянц Е.М. Кузнецова Н.С. Черкиев И

Today, considerable progress in the renal cell carcinoma (RCC) treatment has been made due to development of targeted and immunotherapeutic approaches RCC treatment, especially metastasising carcinoma. In early stages RCC, it is possible use partial or total surgical nephrectomy, but metastases development, range efficient methods dramatically limited. Appearance drugs like PD-1 CTLA-4 receptors their ligands’ inhibitors clinical practice significantly increased survival rate patients with...

e13506 Background: Gliomas are the most common type of primary brain tumors, with a high level recurrence and mortality. The purpose study was to determine expression profile micro-RNA targeting components Hedgehog, Notch, Wnt, EGFR, TGFβ, HIF1α signaling pathways according MirTarBase, miRDB, TargetScan. Methods: included 30 patients (15 women 15 men) aged 27 76 years histologically verified glial tumors. RT2-qPCR method in operational biopsy specimens determined relative 12 micro-RNAs:...

ПРОГНОСТИЧЕСКАЯ ЗНАЧИМОСТЬ МИКРОРНК MIR-22-3P, MIR-107 И MIR-330-3P В ОЦЕНКЕ ВЫЖИВАЕМОСТИ ПАЦИЕНТОВ С ГЛИОМАМИ ВЫСОКОЙ СТЕПЕНИ ЗЛОКАЧЕСТВЕННОСТИАллилуев И.А. 1,2, Пушкин А

e14027 Background: Circulating microRNAs (miRNAs) are currently being investigated as non-invasive diagnostic biomarkers in a wide range of tumors. However, there is still no sufficiently reliable siRNA signature for routine screening patients with glial tumors varying degrees malignancy. Our study aimed to determine the level microRNA pattern blood plasma tumors, which previously showed aberrant expression Methods: Relative twenty miRNAs (hsa-miR-3180, hsa-miR-3180-3p, hsa-miR-6782-5p,...

Introduction. Primary mediastinal large B-cell lymphoma is an aggressive variant of characterized by genetic heterogeneity. First-time therapy for primary usually includes immunochemotherapy. However, a substantial proportion patients do not respond to this therapy. Objective – analyze clinical characteristics refractory taking into account the results targeted next-generation sequencing (NGS). Materials and methods. A 22‑year-old patient with who had responded immunochemotherapy was tested...

ПРЕДИКТОРНАЯ РОЛЬ ЛОКАЛЬНОГО УРОВНЯ ЦИТОКИНОВ В ТЕЧЕНИИ НИЗКОДИФФЕРЕНЦИРОВАННЫХ ГЛИАЛЬНЫХ ОПУХОЛЕЙЗлатник Е.Ю

e15066 Background: Aberrant methylation of CpG sites within promoter regions cancer-related genes is an important epigenetic event in carcinogenesis. The frequency changes different sporadic colorectal cancer (CRC), according to various estimates, varies from 15 80% which allows the use this indicator for development new prognostic biomarkers CRC. Methods: Methylation was quantified at 47 CpG-sites four (MGMT, APC, CDH13 and MLH1) tumors normal tissue using bisulfite pyrosequencing. 25...

Introduction. Colorectal cancer is a frequently diagnosed disease being in the third place among oncological diseases both incidence and mortality. Currently, researchers focus on development of more accessible reliable biomarkers colorectal to overcome problems diagnosis progression prognosis this pathology. Aim. To investigate characteristics microRNA expression circulating tumor cells (CTC) patients with cancer. Materials methods. The study included blood samples from 299 colon cancer,...

e13008 Background: The new 2016 WHO classification of tumors the central nervous system takes into consideration mutational status IDH genes. Research molecular changes in gliomas remain to be an actual issue. We analyzed gene expression some significant pathways gliomas. Methods: 27 patients (12 females, 15 males) aged from 76 years with verified brain (glioblastomas (G4) – 67%, anaplastic astrocytomas and oligoastrocytomas (G3) 11%, (G2) 22%) were investigated. 7 mutations IDH1 5 ones IDH2...

e14549 Background: Gliomas are the most common type of primary brain tumors characterized by pronounced proliferation and aggressive infiltration. The purpose study was to analyze expression genes participating in EGFR, TGF-B, HH/GLI, NOTCH HIF1-alpha signaling pathways, transcription factors HBP1, MSI1/2 demethylating agents samples various glioma types. Methods: included paired (tumor physiologically unchanged neural tissue) obtained from 75 patients with histologically verified glial...

e14550 Background: HIF1-alfa is a powerful regulator of angiogenesis and cell invasion activating wide range oncogenes. Control the HIF1-alpha transcription strictly regulated by many molecular pathways, including miRNAs which can become targets for gene therapy cancer. Methods: The study included 30 patients with histologically verified high-grade gliomas. RNAs were isolated from paired (tumor control) samples using TRIzol reagent (Thermo Fisher, USA). cDNA synthesis was performed Reverta...