A5011083699- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Hepatitis C virus research
- Hepatocellular Carcinoma Treatment and Prognosis
- Neuroendocrine Tumor Research Advances
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Hepatitis B Virus Studies
- Diet and metabolism studies
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
- Diet, Metabolism, and Disease
- Cancer, Lipids, and Metabolism
- Gut microbiota and health
- Mycobacterium research and diagnosis
- Lipid metabolism and biosynthesis
- Organ Transplantation Techniques and Outcomes
- Liver Diseases and Immunity
- Pancreatic and Hepatic Oncology Research
- Bacterial Genetics and Biotechnology
- Adrenal and Paraganglionic Tumors
- Helicobacter pylori-related gastroenterology studies
- Lung Cancer Research Studies
- Lipid metabolism and disorders
- Renal cell carcinoma treatment
University of California, Los Angeles
2015-2024
VA Greater Los Angeles Healthcare System
2016-2024
Hôpital Militaire Moulay Ismail
2024
University of Colorado Denver
2023
University of Colorado Cancer Center
2023
West Los Angeles College
2022
VA West Los Angeles Medical Center
2021
Jackson and Tull (United States)
2021
University Gastroenterology
2017
American Society for Parenteral and Enteral Nutrition
2017
Single-nucleus RNA sequencing (snRNA-seq) measures gene expression in individual nuclei instead of cells, allowing for unbiased cell type characterization solid tissues. We observe that snRNA-seq is commonly subject to contamination by high amounts ambient RNA, which can lead biased downstream analyses, such as identification spurious types if overlooked. present a novel approach quantify and filter droplets experiments, called Debris Identification using Expectation Maximization (DIEM). Our...
Abstract Background Hepatocellular carcinoma (HCC) is a common primary liver cancer with poor overall survival. We hypothesized that there are HCC-associated cell-types impact patient Methods combined single nucleus (snRNA-seq), cell (scRNA-seq), and bulk RNA-sequencing (RNA-seq) data to search for cell-type differences in HCC. To first identify HCC, adjacent non-tumor tissue, normal liver, we integrated single-cell level from healthy cohort ( n = 9 non-HCC samples) collected the Strasbourg...
Background: Patients with cirrhosis and subcentimeter lesions on liver ultrasound are recommended to undergo short-interval follow-up because of the presumed low risk primary cancer (PLC). Aims: The aim this study is characterize recall patterns PLC in patients ultrasound. Methods: We conducted a multicenter retrospective cohort among or chronic hepatitis B infection who had between January 2017 December 2019. excluded history concomitant ≥1 cm diameter. used Kaplan Meier multivariable Cox...
Small noncoding RNAs (sRNAs) regulate gene expression in Escherichia coli by base pairing with mRNAs and modulating translation mRNA stability. The sRNAs DsrA RprA stimulate the of stress response transcription factor RpoS 5' untranslated region rpoS mRNA. In present study, we found that was unstable absence these increased both accumulation half-life Mutations dsrA, rprA, or disrupt predicted sequences reduce also destabilize We accumulates an RNase E mutant strain sRNA and, therefore, is...
Non-alcoholic fatty liver disease (NAFLD) is a fast-growing, underdiagnosed, epidemic. We hypothesise that obesity-related inflammation compromises adipose tissue functions, preventing efficient fat storage, and thus driving ectopic accumulation into the liver.
Although the safety and feasibility of partial adrenalectomy in patients with von Hippel-Lindau syndrome have been established, long-term outcomes not examined. In this study we evaluate recurrence functional a cohort treated for pheochromocytoma followup at least 5 years.
Abstract Birt‐Hogg‐Dubé syndrome (BHDS), caused by germline mutations in the folliculin ( FLCN ) gene, predisposes individuals to develop fibrofolliculomas, pulmonary cysts, spontaneous pneumothoraces, and kidney cancer. The mutation detection rate bidirectional DNA sequencing National Cancer Institute BHDS cohort was 88%. To determine if intragenic deletions/duplications were responsible for families lacking sequence alterations, 23 from 15 unrelated with clinically confirmed but no...
The global obesity epidemic is driving the concomitant rise in nonalcoholic fatty liver disease (NAFLD). To identify new genes involved central functions, we examined RNA-sequence data from 259 patients who underwent morbidly obese bariatric surgery. Of these patients, 84 had normal histology, 40 simple steatosis, 43 steatohepatitis, and remaining 92 varying degrees of NAFLD based on histology. We discovered oligodendrocyte maturation-associated long intergenic noncoding RNA (OLMALINC), a...
Targeting SBPTN1 with siRNA has the potential to treat NASH and prevent HCC.
To reduce the disparity in access to liver transplant (LT), United Network for Organ Sharing implemented an exception policy May 2019, which capped hepatocellular carcinoma (HCC) score median Model End-Stage Liver Disease (MELD) at within donor service area minus 3 points (MMaT-3) after 6-mo wait period. We aimed evaluate how this affected HCC waitlist outcomes.Using data, we analyzed outcomes patients time they received from pre-MMaT era (August 15, 2017, November 2018) and MMaT (June 1,...
Liver X receptors (LXRs) are determinants of hepatic stellate cell (HSC) activation and liver fibrosis. Freshly isolated HSCs from Lxr αβ −/− mice have increased lipid droplet (LD) size, but the functional consequences this unknown. Our aim was to determine whether LXRs link cholesterol retinoid storage in how impacts activation. Primary wild‐type were profiled by gene array during vitro Lipid content quantified high‐performance liquid chromatography mass spectroscopy. treated with nuclear...
Guidelines recommend routine nucleic-acid amplification testing in patients with presumed tuberculosis (TB), but these tests have not been widely adopted. GeneXpert MTB/RIF (Xpert), a novel, semiautomated TB test, has renewed interest this technology, data from low-burden countries are limited.We sought to estimate Xpert's potential clinical and public health impact on empiric treatment, contact investigation, housing undergoing evaluation.We performed prospective, cross-sectional study...
The inflammatory bowel diseases (IBD) are a complex set of chronic gastrointestinal conditions arising from the interplay genetic and environmental factors. This study focuses on noncoding RNA transcripts as potential mediators IBD pathophysiology. One particular gene, interferon γ-antisense 1 (IFNG-AS1), has been consistently observed to be elevated in intestinal mucosa patients with actively inflamed versus healthy controls. builds these observations, demonstrating that second splice...
Aim Cirrhosis is a leading cause of death worldwide, yet there are no well‐established risk stratifying tools for lethal complications, including hepatocellular carcinoma (HCC). Patients with liver cirrhosis undergo routine endoscopic surveillance, providing ready access to duodenal aspirate samples that could be source identifying novel biomarkers. The aim this study was characterize the microbiome and bile acid profiles in aspirates from patients assess feasibility developing biomarkers...
Hepatoma Research is an open access journal and focuses on all topics related to hepatoma. The following articles are especially welcome: pathogenesis, clinical examination early diagnosis of hepatoma, complications their preventions treatments, etc.
The gut microbiome is a key factor in chronic liver disease progression. In prior research, we found that the duodenal was associated with sex, ethnicity, and cirrhosis complications. Here, examined association between diet patients cirrhosis. This study included 51 participants who completed detailed food frequency questionnaire donated biopsies for characterization by 16S ribosomal RNA gene sequencing. Data were analyzed alpha diversity, beta of taxa abundance quality components using...
Abstract Hepatitis C virus ( HCV ) infection is a major cause of chronic liver disease. cure has been linked to improved patient outcomes. In the era direct‐acting antivirals DAA s), become goal, as defined by sustained virological response 12 weeks SVR 12) after completion therapy. Historically, African‐Americans have had lower rates compared White people in interferon era, which attributed high prevalence non‐ CC interleukin 28B IL 28B) type. Less known about association between...
To determine how sustained virological response at 12 wk (SVR12) with direct acting antivirals (DAAs) for the treatment of hepatitis C virus (HCV) infection affects chronic kidney disease (CKD) progression.A retrospective analysis was performed in patients aged ≥ 18 years treated HCV DAAs VA Greater Los Angeles Healthcare System from 2014-2016. The group compared to 2011-2013 who did not undergo treatment, prior introduction DAAs; control matched study terms age, gender, and ethnicity....