A5024237647- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Gut microbiota and health
- Birth, Development, and Health
- Ginseng Biological Effects and Applications
- Gestational Diabetes Research and Management
- Pregnancy and preeclampsia studies
- Advanced Proteomics Techniques and Applications
- Alcohol Consumption and Health Effects
- Eicosanoids and Hypertension Pharmacology
- Stress Responses and Cortisol
- Health, Environment, Cognitive Aging
- Food composition and properties
- Biochemical Analysis and Sensing Techniques
- Aortic Disease and Treatment Approaches
- Exercise and Physiological Responses
- Diet, Metabolism, and Disease
- Cardiac Valve Diseases and Treatments
- Nutritional Studies and Diet
- Phytoestrogen effects and research
- Tryptophan and brain disorders
- Air Quality and Health Impacts
- Regulation of Appetite and Obesity
University of Eastern Finland
2020-2024
Delfin Technologies (Finland)
2022-2024
Metabolomics analysis generates vast arrays of data, necessitating comprehensive workflows involving expertise in analytics, biochemistry and bioinformatics order to provide coherent high-quality data that enable discovery robust biologically significant metabolic findings. In this protocol article, we introduce notame, an analytical workflow for non-targeted profiling approaches, utilizing liquid chromatography–mass spectrometry analysis. We overview lab protocols statistical methods...
The mechanisms by which exercise benefits patients with non-alcoholic fatty liver disease (NAFLD), the most common worldwide, remain poorly understood. A non-targeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics analysis was used to identify metabolic changes associated NAFLD in humans upon intervention (without diet change) across four different sample types-adipose tissue (AT), plasma, urine, and stool. Altogether, 46 subjects participated this randomized controlled...
Abstract The essential role of gut microbiota in health and disease is well recognized, but the biochemical details that underlie beneficial impact remain largely undefined. To maintain its stability, participates an interactive host-microbiota metabolic signaling, impacting phenotypes host. Dysbiosis results alteration certain microbial host metabolites. Identifying these markers could enhance early detection diseases. We report LC–MS based non-targeted profiling demonstrates a large effect...
Abstract Background & Aims Non‐alcoholic fatty liver disease (NAFLD) has been associated with multiple metabolic abnormalities. By applying a non‐targeted metabolomics approach, we aimed at investigating whether serum metabolite profile that associates NAFLD would differ in its association NAFLD‐related risk factors. Methods Results A total of 233 subjects (mean ± SD: 48.3 9.3 years old; BMI: 43.1 5.4 kg/m 2 ; 64 male) undergoing bariatric surgery were studied. Of these participants, 164...
Abstract Epidemiological studies have shown associations between whole-grain intake and lowered disease risk. A sufficient level of to reach the health benefits has not been established, there is limited knowledge about impact on metabolite levels. In this clinical intervention study, we aimed identify plasma urine metabolites associated with two different levels wheat rye correlate them biomarkers. Healthy volunteers ( N = 68) were divided into groups receiving either or in four-week...
Abstract Introduction Maternal metabolism changes substantially during pregnancy. However, few studies have used metabolomics technologies to characterize across gestation. Objectives and methods We applied liquid chromatography–mass spectrometry (LC–MS) based non-targeted determine whether the metabolic profile of serum differs throughout pregnancy between pre-eclamptic healthy women in FINNPEC (Finnish Genetics Preeclampsia Consortium) Study. Serum samples were available from early late...
Non-alcoholic fatty liver disease (NAFLD) pathogenesis remains poorly understood due to the complex metabolic and inflammatory changes in liver. This study aimed elucidate hepatic events related inflammation lipid metabolism their linkage with alterations during NAFLD American lifestyle-induced obesity syndrome (ALIOS) diet-fed mice. Forty-eight C57BL/6J male mice were fed ALIOS diet (n=24) or control chow for 8, 12, 16 weeks. At end of each timepoint, eight sacrificed where plasma...
The current epidemics of cardiovascular and metabolic noncommunicable diseases have emerged alongside dramatic modifications in lifestyle living environments. These correspond to changes our "modern" postwar societies globally characterized by rural-to-urban migration, modernization agricultural practices, transportation, climate change, aging. Evidence suggests that these are related each other, although the social biological mechanisms as well their interactions yet be uncovered....
Long-term lifestyle interventions in childhood and adolescence can significantly improve cardiometabolic health, but the underlying molecular mechanisms remain poorly understood. To address this knowledge gap, we conducted an 8-year diet physical activity intervention a general population of children. The research revealed that influenced 80 serum metabolites over two years, with 17 continuing to be affected after eight years. primarily impacted fatty amides, including palmitic amide,...
Abstract Aims/hypothesis It is not known whether the early-pregnancy metabolome differs in patients with early- vs late-onset gestational diabetes mellitus (GDM) stratified by maternal overweight. The aims of this study were to analyse correlations between metabolites and glycaemic anthropometric characteristics, identify metabolomic alterations that characterise lean women (BMI <25 kg/m 2 ) overweight ≥25 early-onset GDM (E-GDM) or (L-GDM). Methods We performed a nested case–control...
Abstract Psychological stress is a suggested risk factor of metabolic disorders, but molecular mediators are not well understood. We investigated the association between profiles fasting plasma and improvement psychological well-being using non-targeted liquid chromatography-mass spectrometry (LC-MS) platform. The volunteers participating in face-to-face intervention group (n = 60) randomised lifestyle were compared to ones controls 64) baseline 36-week follow-up. Despite modest differences...
Cold-induced brown adipose tissue (BAT) activation is considered to improve metabolic health. In murine BAT, cold increases the fundamental molecule for mitochondrial function, nicotinamide adenine dinucleotide (NAD+), but limited knowledge of NAD+ metabolism during in human BAT exists. We show that serum metabolites salvage pathway (nicotinamide and 1-methylnicotinamide) humans. Additionally, individuals with cold-stimulated have decreased levels from de novo biosynthesis (tryptophan,...
Metabolic dysfunction-associated steatotic liver disease (MASLD), formerly known as non-alcoholic fatty (NAFLD), is the most prevalent globally, yet no therapies are approved. The effects of Escherichia coli Nissle 1917 expressing aldafermin, an engineered analog intestinal hormone FGF19, in combination with dietary change were investigated a potential treatment for MASLD. MASLD was induced C57BL/6J male mice by American lifestyle-induced obesity syndrome diet and then switched to standard...
Heavy alcohol use has been associated with altered circulating metabolome. We investigated whether changes in the metabolome precede incident diagnoses of alcohol-related diseases.This is a prospective population-based cohort study where participants were 42- to 60-year-old males at baseline (years 1984 1989). Subjects who received diagnosis for an disease during follow-up defined as cases (n = 92, mean 13.6 years before diagnosis). Diagnoses obtained through linkage national health...
SummaryBackground & aimsDiet and weight loss affect circulating metabolome. However, metabolite profiles induced by different maintenance diets underlying longer term remain unknown. Herein, we investigated after-weight-loss metabolic signatures of two isocaloric 24-wk differing in satiety value due to dietary fibre, protein fat contents identified features that associated with successful maintenance.MethodsNon-targeted LC-MS metabolomics approach was used analyse plasma metabolites 79 women...
Abstract Background Ghrelin may influence several alcohol‐related behaviors in animals and humans by modulating central and/or peripheral biological pathways. The aim of this exploratory analysis was to investigate associations between ghrelin administration the human circulating metabolome during alcohol exposure nontreatment seeking, heavy drinking individuals with use disorder (AUD). Methods We used serum samples from a randomized, crossover, double‐blind, placebo‐controlled laboratory...
Abstract Background Molecular mechanisms underlying the beneficial effects of long-term lifestyle interventions on cardiometabolic health during childhood and adolescence remain largely unknown. Such information would provide valuable insights into prevention diseases since childhood. We therefore studied for first time a diet physical activity (PA) intervention serum metabolites in general population children. Methods carried out an 8-year, nonrandomized, controlled trial sample 490...
Metabolomics analysis generates vast arrays of data, necessitating comprehensive workflows involving expertise in analytics, biochemistry and bioinformatics, order to provide coherent high-quality data that enables discovery robust biologically significant metabolic findings. In this protocol article, we introduce NoTaMe, an analytical workflow for non-targeted profiling approaches utilizing liquid chromatography&ndash;mass spectrometry analysis. We overview lab protocols statistical...
Abstract The essential role of gut microbiota in health and disease is well-recognized, but the biochemical details underling beneficial impact remain largely undefined. Dysbiosis bacteria results alteration certain microbial host metabolites, identifying these markers could enhance early detection diseases. We report LC-MS based non-targeted metabolic profiling to demonstrate a large effect on mammalian tissue metabolites. It was hypothesized that influences overall biochemistry metabolome...
Abstract BackgroundThe essential role of gut microbiota in health and disease is well recognized, but the biochemical details that underlie beneficial impact remain largely undefined. To maintain its stability, participates an interactive host-microbiota metabolic signaling, impacting phenotypes host. Dysbiosis bacteria results alteration certain microbial host metabolites, identifying these markers could enhance early detection diseases. Herein, we report LC-MS based non-targeted profiling...
Abstract Background Calcific aortic valve disease (CAVD) is the most common valvular heart in Western world. CAVD ranging from mild sclerosis to severe obstructive stenosis (AS). The development of AS has been associated with several risk factors including age, sex and hypertension. However, there limited knowledge about that predict stenosis. Purpose We investigated if circulating metabolite profile can Finnish males. Methods did a non-targeted LC-MS metabolomics analysis baseline...