A5073095604- Hepatitis C virus research
- Hepatitis Viruses Studies and Epidemiology
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Liver Disease Diagnosis and Treatment
- Veterinary medicine and infectious diseases
- Liver Disease and Transplantation
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Liver Diseases and Immunity
- interferon and immune responses
- Virus-based gene therapy research
- HIV Research and Treatment
- Systemic Lupus Erythematosus Research
- Drug-Induced Hepatotoxicity and Protection
- Influenza Virus Research Studies
- Food Quality and Safety Studies
- Gastrointestinal Bleeding Diagnosis and Treatment
- Trace Elements in Health
- Milk Quality and Mastitis in Dairy Cows
- Cancer Research and Treatments
- Polyomavirus and related diseases
- Food Safety and Hygiene
- Viral-associated cancers and disorders
- Plant Virus Research Studies
Fujita Health University Nanakuri Memorial Hospital
2013-2022
Fujita Health University Hospital
2019-2022
Fujita Health University
2013-2022
Otemae Hospital
2018
Ichinomiya Kenshin College
2004-2006
Nishijin Hospital
2005-2006
Centers for Disease Control and Prevention
2001-2004
National Center for Infectious Diseases
2004
Nagoya City University
1993-2001
Nishikawa Hospital
1998
Hepatitis B virus (HBV) is classified into genotypes A–F, which important for clinical and etiological investigations. To establish a simple genotyping method, 68 full‐genomic sequences 106 S gene were analyzed by the molecular evolutionary method. HBV with sequence consistent genetic analysis using sequence. After alignment of sequences, genotype specific regions are identified digested restriction enzymes, Hph I, Nci Alw Ear Nla IV. This system fragment length polymorphism (RFLP) was...
The hepatitis C virus (HCV) genomic database is expanding rapidly.There a need to provide an updated phylogenetic tree analysis based on the complete coding region of HCV.All available HCV genome sequences in databases through October 2010 were analyzed.The assignment all known up-to-date confirmed previous six major genotypes and one new sequence, which have been provisionally assigned as subtype 7a. New recombinant forms HCV, although uncommon, detected found different crossover...
Nuclear factor κB (NF‐κB) is a transcription that critical for the inducible expression of multiple cellular and viral genes. DNA binding activity essential its function. Here, we report gold compounds, especially aurothioglucose (AuTG), have strong inhibitory effect on NF‐κB‐DNA binding. Our finding also reveals Zn 2+ necessary component NF‐κB ion can efficiently block binding, presumably through oxidation cysteins associated with zinc. This redox mechanism may provide an explanation...
Abstract To determine hepatitis C virus (HCV) genotype distribution in China, a total of 148 HCV RNA positive serum samples were collected from nine geographic areas and subjected to RT‐PCR followed by direct DNA sequencing phylogenetic analysis the core, E1, NS5B regions. was genotyped 139 (93.9%) samples. Among them subtype 1b most predominant [66% (92/139)] 2a [14% (19/139)]. Of 92 isolates, 35 (38%) 30 (33%) formed two clusters, designated groups A B. Group prevalent throughout while...
The complete genome sequences of hepatitis D virus (HDV) strains isolated from three Yucpa Amerindians in Venezuela were determined and found to be genotype III. Comparison these III demonstrated the presence a hypervariable region containing numerous substitutions, insertions/deletions highly conserved self-cleavage domains, which have been reported previously for genotypes I II. Amino acid changes within first 90 amino acids antigen (HDAg) sequences, while remainder HDAg-coding sequence...
The complete genomic sequence of hepatitis A virus (HAV) CF53/Berne strain was determined. Pairwise comparison with other HAV sequences demonstrated that the isolate is most closely related to single genotype VII strain, SLF88. This close relationship confirmed by phylogenetic analyses different regions, and pronounced within capsid region. These data indicated SLF88 isolates are more each than subtypes IA IB. histogram genetic differences between strains revealed four separate peaks....
The analysis of molecular phylogenies estimated from the gene sequences sampled viruses can provide important insights into epidemiological processes.The demographic and migration histories prevalent hepatitis C virus (HCV) subtypes 1a 1b were inferred viral in 5 countries. Estimated analyzed by use methods based on parsimony coalescent theory.The suggested that global subtype epidemics are geographically structured, with asymmetrical movement HCV strains among indicated infections United...
Acute hepatitis C virus (HCV) is typically defined as new viremia and antibody seroconversion. Rates immunologic correlates of clearance have therefore been based on only in individuals who initially had an response. We sought to characterize the immunological patients with acute their sexual contacts. prospectively determined CD4(+) CD8(+) cytotoxic T-lymphocyte responses index HCV contacts developed infection, either or without spontaneous clearance, well those never viremia. Responses...
We investigated the migration patterns of hepatitis C virus (HCV) in China. Partial E1 and/or NS5B sequences from 411 volunteer blood donors sampled 17 provinces and municipalities located five large regions, north-northeast, northwest, southwest, central south, southeast, were characterized. The classified into eight subtypes (1a, n = 3; 1b, 183; 2a, 83; 3a, 30; 3b, 44; 6a, 55; 6n, 10; 6v, 1) a new subtype candidate. Bayesian evolutionary analysis by sampling trees major revealed distinct...
The 5′‐untranslated region (5′‐UTR) sequences of 33 GB virus C/hepatitis G (GBV‐C/HGV) obtained from different geographic areas were determined through reverse‐transcription polymerase chain reaction and dideoxy termination sequencing, the alignment sequences, estimation number nucleotide substitution per site, construction phylogenetic trees. 5′‐UTR GBV‐HGV was found to be heterogeneous, with 70.9–99.5% homology. Three distinct branches observed consistently in all GBV‐C is prototype for...
We determined the sequence of hepatitis delta virus (HDV) genome in 40 Japanese patients, most whom were from Miyako Islands, Okinawa, Japan. Consensus sequences 33 HDV full genomes out a total patients by directly sequencing four partially overlapping PCR products. Phylogenetic tree analysis classified these complete as genotype I (two patients), IIa (one patient) and IIb (30 patients). Among 30 there two clusters genetic variants. One group consisted six isolates showing significant...
The complete genome sequence of the only identified genotype VII hepatitis A virus (HAV), strain SLF88, was obtained from PCR amplicons generated by a modified long approach. There 90% nucleotide identity in 5' untranslated region compared to other known HAV sequences. In remainder containing open reading frame, there about 85% human genotypes IA and IB 80% simian V. Compared HM-175, capsid amino acids were highly conserved, with four homologous acid changes, while an increasing number...
The complete genome sequences of hepatitis B virus (HBV) from 12 HBV-infected Yucpa Indians Venezuela, a group with highly endemic HBV, were amplified and sequenced. isolates closely related to each other, 98.6-100% nucleotide identity. A phylogenetic tree based on the indicated clearly that they genotype F. Three individuals had evidence infection two different HBV deletion mutants. In individuals, three amino acid was identified just prior 'a' determinant loop S region. third individual...
Subtype 1b is the most common strain of Hepatitis C virus (HCV) in China. Here, molecular epidemiology and epidemic history this were investigated by conducting phylogenetic population genetic analyses E1 NS5B gene sequences sampled from nine Chinese cities. The analysis indicated presence two clusters strains that did not include reference other countries, suggesting these represent independent chains HCV transmission within remaining isolates more closely related to countries. date origin...
Aim To characterize hepatitis E in M ie prefecture and to investigate whether raw pig liver sold as food is contaminated with virus ( HEV ) strains similar those recovered from patients. Methods Seventeen patients sporadic acute treated 2004 2012 were studied. A total of 243 packages regional grocery stores tested for the presence RNA . The partial genomic sequences human swine isolates determined subjected phylogenetic analyses. Results 17 segregated into genotype 3 n = 15) 4 2), 15 further...
Here, the complete genome sequences for three hepatitis C virus (HCV) variants identified from China and belonging to genotype 6 are reported: km41, km42 gz52557. Their entire lengths were 9430, 9441 9448 nt, respectively; 5' untranslated regions (UTRs) contained 341, 342 339 followed by single open reading frames of 9045, 9045 9057 3' UTRs, up poly(U) tracts, 41, 51 52 respectively. Phylogenetic analyses showed that km41 is classified into subtype 6k 6n. Although gz52557 clustered distantly...
Abstract Background Since previous studies have investigated the population dynamics of Japan‐indigenous genotype 3 hepatitis E virus ( HEV ) using sequences, more nucleotide sequences been determined, and new techniques developed for such analysis. Aims To prevent future epidemic in Japan, this study aimed to elucidate cause past expansion. Methods The history was determined coalescent analysis framework. Bayesian skyline plot BSP estimate phylogeny with relaxed molecular clock models were...
A 67-year-old male living in Tsu city, Mie prefecture, Japan was referred to our hospital for further examination of acute liver injury and diagnosed as having clinical hepatitis E virus (HEV) infection January 2010. The HEV strain (HE-JA11-1701) isolated from the patient belonged genotype 3 European-type subgenotype 3e. It presumed that had been infected a wild boar (Sus scrofa leucomystax) because he consumed meat/viscera captured himself hunter approximately 2 months before disease onset....
Hepatitis E virus (HEV) genotype 4 has mainly been isolated from sporadic hepatitis cases and swine in Asian countries. We analysed the origin global dispersal history of using a Bayesian phylogeographical approach.The 412-nucleotide sequences open reading frame 2 (47 Japanese, 40 Chinese, 1 Indian, 8 Indonesian, Korean, Taiwanese, Danish Italian), which sampling date location were known, collected. Evolutionary rate, divergence time, demographic growth phylogeography co-estimated...
Although TT virus (TTV) was isolated from a cryptogenic posttransfusion hepatitis patient, its pathogenic role remains unclear. It has been reported that the majority of healthy population is infected with TTV. To elucidate differences between TTV infection in patients liver diseases and population, quantification system developed. DNA quantified by real-time detection PCR (RTD-PCR) assay on an ABI Prism 7700 sequence detector. With this system, 78 C (HCV)-infected (63 elevated serum alanine...