A5108059004- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- RNA Research and Splicing
- Atherosclerosis and Cardiovascular Diseases
- Bone Metabolism and Diseases
- Elbow and Forearm Trauma Treatment
- Osteoarthritis Treatment and Mechanisms
- interferon and immune responses
- Orthopedic Surgery and Rehabilitation
- Medicinal Plant Pharmacodynamics Research
- Bone fractures and treatments
- Galectins and Cancer Biology
- RNA regulation and disease
- Natural product bioactivities and synthesis
- HIV Research and Treatment
- Cancer, Hypoxia, and Metabolism
- Cholesterol and Lipid Metabolism
- Inflammatory mediators and NSAID effects
- Per- and polyfluoroalkyl substances research
- Shoulder Injury and Treatment
- Autophagy in Disease and Therapy
- Toxic Organic Pollutants Impact
- Molecular Biology Techniques and Applications
- Inflammasome and immune disorders
Zhengzhou Children's Hospital
2023-2024
Zhengzhou University
2023-2024
Ningbo First Hospital
2017-2023
First Affiliated Hospital of Anhui Medical University
2019-2023
Anhui Medical University
2019-2023
First Affiliated Hospital of Zhengzhou University
2015-2021
Tianjin Huanhu Hospital
2020
Tianjin Medical University
2020
Dalian Medical University
2016
Zhejiang University
2015-2016
Oxidized low-density lipoprotein (oxLDL) indu-ces macrophage inflammation and lipid uptake, serves important roles in the development of atherosclerosis. The long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (neat1) has two isoforms; longer isoform, neat1_2, mediates formation subnuclear structures called paraspeckles. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), western blotting protein immunoprecipitation (RIP), revealed that oxLDL induced...
Atherosclerosis (AS), characterized by cholesterol overloaded-macrophages accumulation and plaque formation in blood vessels, is the major cause of cardiovascular disease. Transactive response DNA-binding protein∼43 kDa (TDP43) has recently been identified as an independent driver neurodegenerative diseases through triggering inflammatory response. This study investigated whether TDP43 involved AS development, especially macrophages-mediated-foam cell responses.Transactive expressions...
α-l-Fucosidase 1 (FUCA1), a lysosomal enzyme that catalyses the hydrolytic cleavage of terminal fucose residue, has been reported to be involved in tumorigenesis. However, clinical significance and biological roles FUCA1 glioma remain largely unknown. We analyzed expression according data Oncomine, The Cancer Genome Atlas, Chinese Glioma Atlas databases further verified with immunohistochemistry real-time PCR analysis tissues. results showed overexpression was significantly associated...
Increasing evidence suggested the involvement of microRNA (miR)‑146a in pathogenesis multiple diseases, including atherosclerosis, bacterial infection and cancer. However, mechanism by which miR‑146a is regulated macrophages exposed to oxidized low‑density lipoprotein (oxLDL) has remained elusive. The present study aimed explore molecular pathway regulation response oxLDL. Human THP‑1 were pre‑treated with small interfering RNA specific for scavenger receptors or pharmacological inhibitors...
Dendritic cells (DCs) have been implicated in the pathogenesis of atherosclerosis (AS). Whereas pathogenic role oxidized low-density lipoprotein (oxLDL) development and progression AS has recognized previously, contribution microRNA-155 (miR-155) to was previously not fully understood. It had also noted that miR-155 levels were upregulated by oxLDL various cell types under different (patho)physiological conditions, but its underlying mechanisms examiend detail. Thus, present study, we...
Abstract — Atherosclerosis (AS) is the main cause of cerebrovascular diseases, and macrophages play important roles in atherosclerosis. DExH-Box helicase 9 (DHX9), as a member DExD/H-box RNA superfamily II, identified an autoantigen sera systemic lupus erythematosus patients to trigger inflammation. The aim this study was investigate whether DHX9 involved AS development, especially macrophages-mediated-inflammatory responses. We find that expression significantly increased oxLDL or...
Abstract Bone regeneration, as a physiological process of bone formation, is regulated by multiple cytokines. Long noncoding RNAs are involved in the progress formation. The present study investigated role which ZBED3‐AS1 acts to control differentiation mesenchymal stem cells (MSCs) and regeneration. Bioinformatics prediction dual luciferase reporter gene assay identified putative binding sites on 3′‐untranslated region interleukin‐1β (IL‐1β). Then, RNA immunoprecipitation chromatin assays...
This study investigated the clinical and functional outcomes of children with distal both-bone forearm fractures treated by fixation radius only compared to both ulna.
Abstract Long non-coding RNAs (lncRNAs) have been proposed to play pivotal roles in the tumorigenesis of various malignant tumors. Previous studies found that lncRNA LBX2-AS1 is involved progression However, currently, expression and exact mechanism glioma remain unclear. In this study, using online-available datasets combined with clinical tissues collected, we was significantly increased negatively correlated prognosis glioma. vitro functional assays such as CCK-8, Annexin V, transwell...
The purpose of this study was to investigate the clinical and functional outcomes early versus delayed treatment pediatric lateral condylar fractures humerus with a displacement greater than 2 mm.Sixty-seven children treated surgically at our hospital from March 2016 September 2021 for fracture > mm were retrospectively analyzed. divided into two groups where surgery consisted patients being operated on within 24-h post-injury (n = 36) after 31). Clinical results compared between...
Survival of mesenchymal stem cells in the bone marrow is essential for microenvironment homeostasis, but molecular mechanisms remain poorly understood. RIPK1 has emerged as a critical molecule programmed cell death tissue homeostasis. However, little known about regulation on (MSCs). Here, we have investigated first time role MSCs. We found that knockdown suppressed proliferation, differentiation, and migration Furthermore, resulted opening mitochondrial permeability transition pore (mPTP)...
Objective To explore the inhibitory effect of small interfering RNA(siRNA) on Cathepsin K expression and growth invasion ability osteosarcoma cells. Methods A mRNA- targeting siRNA was transfected into MG63 cells with lipidosme. Reverse transcriptasepolymerase chain reaction(RT- PCR)and Western blotting were used to detect mRNA protein respectively. Methyl thiazol tetrazolium(MTT)and Transwell assays applied measure proliferation cells. Results The levels in siRNA(0.05± 0.16...
Objective Objective to investigate the effects of tanshinone Ⅱ A on proliferation, apoptosis and cell cycle distribution osteoblasts MC3T3-E1. Methods The MC3T3-E1 were divided into four groups: blank control group, low concentration group (5×10-3 mg/L), medium (5×10-2 mg/L) high (5×10-1 mg/L). The was treated with routine culture without any drugs, while middle cells, methyl thiazol tetrazolium (MTT) assay used detect proliferation thyroid cancer flow cytometry...