A5023611720- Plant tissue culture and regeneration
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Plant Genetic and Mutation Studies
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Seed Germination and Physiology
- Analytical Chemistry and Sensors
- Transgenic Plants and Applications
- bioluminescence and chemiluminescence research
- Advanced Chemical Sensor Technologies
- SARS-CoV-2 detection and testing
- Electrochemical sensors and biosensors
- Insect and Pesticide Research
- Pesticide Residue Analysis and Safety
- Viral Infectious Diseases and Gene Expression in Insects
- Toxin Mechanisms and Immunotoxins
- Wheat and Barley Genetics and Pathology
- Food Quality and Safety Studies
- Plant Virus Research Studies
- Plant Pathogens and Resistance
- Plant Parasitism and Resistance
- Listeria monocytogenes in Food Safety
- Mitochondrial Function and Pathology
Agricultural University of Athens
2015-2024
Cell Technology (China)
2023
Centre for Research and Technology Hellas
2023
Ministry of National Defence
2020
Bioanalytica (Switzerland)
2017
Technical University of Munich
1992-1994
One of the key challenges recent COVID-19 pandemic is ability to accurately estimate number infected individuals, particularly asymptomatic and/or early-stage patients. We herewith report proof-of-concept development a biosensor able detect SARS-CoV-2 S1 spike protein expressed on surface virus. The based membrane-engineered mammalian cells bearing human chimeric antibody. demonstrate that attachment membrane-bound antibodies resulted in selective and considerable change cellular bioelectric...
The availability of antigen tests for SARS-CoV-2 represents a major step the mass surveillance incidence infection, especially regarding COVID-19 asymptomatic and/or early-stage patients. Recently, we reported development Bioelectric Recognition Assay-based biosensor able to detect S1 spike protein expressed on surface virus in just three minutes, with high sensitivity and selectivity. working principle was established by measuring change electric potential membrane-engineered mammalian...
Crocus sativus L. has various pharmacological properties, known for over 3600 years. These properties are attributed mainly to biologically active substances, which belong the terpenoid group and include crocins, picrocrocin safranal. The aim of current work was examine effects crocins (CRCs) their methyl ester derivate dimethylcrocetin (DMCRT) on glioblastoma rhabdomyosarcoma cell lines, in terms cytotoxicity gene expression, implicated proapoptotic survival pathways. Cell assessed with...
Prostate-specific antigen (PSA) is the established routine screening tool for detection of early-stage prostate cancer. Given laboratory-centric nature process, development a portable, ultra rapid high-throughput system PSA highly desirable. In this study, an advancedpoint-of-care in human serum was developed based on cellular biosensor where cell membrane modified by electroinserting specific antibody against PSA. Thirty nine samples were used validation biosensory detection. Samples...
Aflatoxins are highly toxic fungal secondary metabolites that often contaminate food and feed commodities. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was fabricated by immobilizing monoclonal AFB1 antibodies onto a screen-printed gold electrode modified with carbo-methyldextran N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide cross-linking. interfacial modelling biomolecular recognition suggested reasonably interpreted....
We developed a novel artificial neural network (ANN) system able to detect and classify pesticide residues. The ANN is coupled, in customized way, cellular biosensor operation based on the bioelectric recognition assay (BERA) simultaneously eight samples three minutes. was using data (time series) of electrophysiological responses different cultured cell lines against groups (carbamates, pyrethroids, organophosphates). Using system, we were correctly presence investigated with an overall...
Membrane engineering is a generic methodology for increasing the selectivity of cell biosensor against target molecule, by electroinserting target‐specific receptor‐like molecules on surface. Previous studies have elucidated biochemical aspects interaction between various analytes (including viruses) and their homologous membrane‐engineered cells. In present study, purified anti‐biotin antibodies from rabbit antiserum along with in‐house prepared biotinylated bovine serum albumin (BSA) were...
Population growth and increased production demands on fruit vegetables have driven agricultural to new heights. Nevertheless, agriculture remains one of the least optimized industries, with laboratory tests that take days provide a clear result chemical level produce. To address this problem, we developed tailor-made solution for industry can allow multiple field key pesticides, based bioelectric cell biosensor measurement membrane potential changes, according principle Bioelectric...
The present study describes an artificial neural network (ANN) system that uses a cell-based biosensor based on the Bioelectric Recognition Assay (BERA) methodology, for detection and classification of pesticide residues in food commodities. insecticidal compounds carbaryl chlorpyrifos as well pyrethroid group were used models training ANN. was neuroblastoma N2a cells, which are targets pesticides due to inhibition enzyme acetylcholine esterase by them. response different concentrations...
Abstract The efficiency of 14 commercial cultivars melon ( Cucumis melo L.) for callus induction, plant regeneration and somatic embryogenesis under different photosynthetic photon flux densities (PPFDs) (150 or 50μmol m −2 s −1 ) was investigated. Cotyledonal explants were cultured on a Murashige Skoog (MS) medium supplemented either with 9.0 μM 2,4‐dichlorophenoxyacetic acid 23.2μM kinetin 0.05 0.26 6‐benzyladenine the induction shoots, respectively. For embryo maturation root growing...