A5103132008- Autophagy in Disease and Therapy
- Ginseng Biological Effects and Applications
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Renal Diseases and Glomerulopathies
- DNA Repair Mechanisms
- Sirtuins and Resveratrol in Medicine
- Mast cells and histamine
- Ferroptosis and cancer prognosis
- Calcium signaling and nucleotide metabolism
- Pancreatic and Hepatic Oncology Research
- Gut microbiota and health
- Cancer Mechanisms and Therapy
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Chemotherapy-induced organ toxicity mitigation
- Nuclear Receptors and Signaling
- Cardiac Ischemia and Reperfusion
- Urinary Tract Infections Management
- Cell death mechanisms and regulation
- PI3K/AKT/mTOR signaling in cancer
- Biological and pharmacological studies of plants
- Cytokine Signaling Pathways and Interactions
- Flavonoids in Medical Research
Affiliated Hospital of Hangzhou Normal University
2019-2025
Liaocheng People's Hospital
2025
Hangzhou First People's Hospital
2024
Anhui Medical University
2019-2022
Hangzhou Normal University
2020-2021
Kailuan General Hospital
2021
First Hospital of Qinhuangdao
2017-2020
Soochow University
2015-2019
Second Affiliated Hospital of Zhengzhou University
2019
Academia Sinica
2011
Background and Purpose: RAS mutations limit the effectiveness of anti-epidermal growth factor receptor (EGFR) monoclonal antibodies in combination with chemotherapy for metastatic colorectal cancer (mCRC) patients. Therefore, new cell death forms have focused on identifying indirect targets to inhibit Ras-induced oncogenesis. Recently, emerging evidence has shown potential triggering ferroptosis therapy, particularly eradicating aggressive malignancies that are resistant traditional...
Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation. Currently, the therapeutic role of ferroptosis on cancer gaining increasing interest. Baicalin an active component in
Erianin, a natural product derived from Dendrobium chrysotoxum Lindl , has been proved to play antitumor activity in various cancers. However, the effects and molecular mechanisms of erianin bladder cancer cells remain unexplored. In this study, we found that triggered cell death cycle arrest cells. Then demonstrated could promote accumulation lethal lipid-based reactive oxygen species (ROS) depletion glutathione (GSH), suggesting induction ferroptosis. further ferroptosis inhibitor...
Baicalin, as a natural active ingredient extracted and isolated from the traditional Chinese medicine Scutellaria baicalensis Georgi., has been potentially used in various areas for its antioxidative, antitumor, anti-inflammatory, anti-proliferative activities. Although several studies have reported antitumor effects of baicalin against cancer types, beneficial on lung not yet elucidated. Therefore, therapeutic molecular mechanisms cell lines H1299 H1650 were investigated. Here, results...
Abstract 5-Fluorouracil (5-FU) is known as a first-line chemotherapeutic agent against colorectal cancer (CRC), but drug resistance occurs frequently and significantly limits its clinical success. Our previous study showed that the protocadherin 17 ( PCDH17 ) gene was methylated functioned tumor suppressor in CRC. However, relationship between 5-FU CRC remains unclear. Here, we revealed more highly expressed 5-FU-sensitive tissues than 5-FU-resistant tissues, high expression of correlated...
Abstract Beclin 1 is a well-established core mammalian autophagy protein that embryonically indispensable and has been presumed to suppress oncogenesis via an autophagy-mediated mechanism. Here, we show prenatal primary cytoplasmic but rapidly relocated into the nucleus during postnatal development in mice. Surprisingly, deletion of beclin vitro human cells did not block response, attenuated expression several DNA double-strand break (DSB) repair proteins formation complexes, reduced ability...
Colorectal cancer is a malignant tumor of the digestive system with high morbidity and mortality. 5-fluorouracil remains widely used chemotherapeutic drug in treatment advanced colorectal cancer, but chemotherapy drugs are prone to develop resistance, p53 deletion or mutation an important reason for resistance cells 5-fluorouracil. β-elemene has been proved have potential reverse mechanism unknown. This study aimed investigate effect drug-resistant p53-deficient HCT116p53-/-, determine...
Objective: Erianin has potential anticancer activities, especially against lung cancer. The specific mechanisms underlying the anti-cancer effects, including molecular targets and signaling pathways in cancer, remain poorly understood necessitate further investigation. Methods: Lung cancer cell viability was evaluated using CCK-8 assay. Flow cytometry used to examine effects of erianin on apoptosis cycle progression. mRNA sequencing metabolomics analysis were utilized explore erianin-induced...
Abstract Background Ovarian cancer (OC) is recognized as one of the deadliest forms gynecological cancer, approximately two-thirds patients have already developed metastasis when they are diagnosed. The function transmembrane protein 176B (TMEM176B) in progression OC remains elusive. This study aimed to investigate role and molecular mechanism TMEM176B on proliferation metastasis. Method Expression normal tissues were determined from TCGA, GTEx, CPTAC databases, verified by patient-derived...
Abstract Autophagy is essentially a metabolic process, but its in vivo role nuclear radioprotection remains unexplored. We observed that ex autophagy activation reversed the proliferation inhibition, apoptosis and DNA damage irradiated hematopoietic cells. In improved bone marrow cellularity following radiation exposure. contrast, defective conditional mouse model worsened injury, reactive oxygen species (ROS) accumulation caused by Strikingly, an absence or reduction regulatory proteins...
Ropivacaine is a commonly used local anaesthetic, but its side effects remain largely unknown. In the present study, we investigated of ropivacaine in human neuronal SH-5Y5Y cells. We show that 0.5% and 1% could cause fission-like mitochondrial morphological changes. exclusively induces fission protein DRP1, generation ROS causes dysfunction including decreasing membrane potential, activity cytochrome C oxidase ATP production. The appear to be dependent on DRP1 expression as silencing cells...
Autophagy protects hematopoietic cells from radiation damage in part by promoting DNA repair. However, the molecular mechanisms which autophagy regulates repair remain largely elusive. Here, we report that this radioprotective effect of depends on STAT3 signaling murine bone marrow mononuclear (BM-MNCs). Specifically, found activation and nuclear translocation BM-MNCs were increased with an mTOR inhibitor decreased knockout gene Atg7. The autophagic regulation is likely mediated induction...
Abstract Leukemia cells are superior to hematopoietic with a normal differentiation potential in buffering cellular stresses, but the underlying mechanisms for this leukemic advantage not fully understood. Using CRISPR/Cas9 deletion of canonical autophagy-essential gene Atg 7, we found that erythroleukemia K562 armed two sets autophagic machinery. Alternative mitophagy is functional regardless whether mechanism intact or disrupted. Although autophagy defects attenuated cell cycling,...
Sirtuin protein family member 3 (Sirt3) has been suggested as a positive regulator in alleviating oxidative stress by acting on the mitochondrial antioxidant machinery solid tumors; however, its role and regulation hematological malignancies poorly understood. Here, we show that contrary to what reported tumors, K562 leukemia cells elevated Sirt3 was associated with stress, depletion of decreased reactive oxygen species (ROS) generation lipid oxidation, but increased ratio reduced...
Purpose: To investigate the correlation between expression of PD-L1, SOCS3 and immune-related biomarkers CD276, CD4, CD8 in hepatocellular carcinoma (HCC) further determine relationship with clinicopathologic characteristics prognostic value their co-expression HCC patients. Methods: We assessed SOCS3, CD4 by immunohistochemistry tumor tissue from 74 patients who underwent curative hepatectomy. Results: High PD-L1 was significantly associated high Edmondson grade (p<0.01) elevated enzyme...
Dichloroacetate (DCA) is an inhibitor of pyruvate dehydrogenase kinase, which promotes the flux carbohydrates into mitochondria and enhances aerobic oxidation glucose. DCA has previously been demonstrated to exhibit antitumor properties. The present study revealed that treatment with induced increased levels autophagy‑associated proteins in esophageal squamous carcinoma cells while minimally affecting apoptosis. examined localization light chain (LC)‑3 by adenovirus infection a green...
Abstract Previous studies show that the proliferation of human mesangial cells (HMCs) played a significant part in pathogenesis Henoch‐Schönlein purpura nephritis (HSPN). The aim this study was to explore HMCs induced by IgA1 isolated from sera HSP patients. were cultured three different types media, including patients with (HSP group), healthy children (healthy group) and medium (control group). incubated determined cell counting kit‐8 assay bromodeoxyuridine incorporation. expression...
<title>Abstract</title> Patients with end-stage liver disease often have intestinal flora dysbiosis. Exploring the causal relationship between abundance and cirrhosis or cancer is significant. In this study, we first demonstrated was detrimental to by two-sample Mendelian randomization (MR) analysis. Then, used bidirectional MR verify two-way gut (including 16 classes, 32 families, 119 genera, 20 orders, 9 phyla) diseases. Inverse variance weighted (IVW) primary method for examining...
Acute ischemic stroke (CIS) is a high-risk condition among the elderly, and intravenous thrombolytic therapy (ITT) most effective means for it. However, ITT prone to induce hemorrhagic transformation (HT) that further threatens life health of patients. As paramount substances in cardiovascular cerebrovascular diseases, adipocyte factor (Apelin) serine protease inhibitor (Vaspin) are strongly bound up with CIS.To analyze predictive significance Apelin Vaspin on HT CIS patients after offer...
Abstract Background Chemokine (C–C motif) receptor 2 (CCR2) is involved in important physiological and pathological processes, such as inflammation autoimmune diseases. Abnormal immune inflammatory responses play a critical role the development progression of IgA nephritis (IgAN). However, CCR2 IgAN unknown. Methods Fifteen children who were diagnosed by kidney biopsy provided tissue, blood urine samples, age-matched healthy control subjects (blood donators n = 12; tissue 8) included....