A5077121703- Animal Virus Infections Studies
- Microbial infections and disease research
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Aquaculture disease management and microbiota
- Viral Infections and Immunology Research
- Mosquito-borne diseases and control
- Streptococcal Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Microbial Natural Products and Biosynthesis
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Fungal Biology and Applications
- Cancer-related gene regulation
- Heavy Metal Exposure and Toxicity
- Salmonella and Campylobacter epidemiology
- Cellular transport and secretion
- HVDC Systems and Fault Protection
- Plant Virus Research Studies
- Inflammasome and immune disorders
- RNA regulation and disease
- Biosensors and Analytical Detection
- Viral Infections and Vectors
- Immunotoxicology and immune responses
- Cancer Genomics and Diagnostics
Sichuan Agricultural University
2013-2025
Ministry of Agriculture and Rural Affairs
2024-2025
Sichuan Center for Disease Control and Prevention
2025
Science and Technology Department of Sichuan Province
2025
After being discovered for the first time in China 2017, porcine reproductive and respiratory syndrome virus (PRRSV) NADC34-like strains have become prevalent strain of PRRSV certain regions China. Our previous study showed that reduced Ingelvac PRRS MLV vaccination dosages against NADC30-like CF had a better protection effect than normal dosage. However, protective is unclear. Therefore, this compared effectiveness 0.1 1 infection using commercial vaccines, MLV, which been widely utilized...
ABSTRACT Porcine deltacoronavirus (PDCoV) is an enteric pathogen that burdens the global pig industry and a public health concern. The development of effective antiviral therapies necessary for prevention control PDCoV, yet to date, there are few studies on therapeutic potential PDCoV-neutralizing antibodies. Here, we investigate novel monoclonal antibody (mAb 4A6) which targets PDCoV S1 protein effectively neutralizes both pre- post-attachment cells, with IC50 values 0.537 8.487 µg/mL,...
Human antigen R (HuR) is an RNA-binding protein that regulates the post-transcriptional reaction of its target mRNAs. HuR a critical factor in cancer development and has been identified as potential many models. It participates viral life cycle by binding to RNAs. In prior work, we used CRISPR/Cas9 screening identify prospective host facilitating Japanese encephalitis virus (JEV) infection. The gene was successfully knocked out U251 cell lines using gene-editing system, with no significant...
Pasteurella multocida, a zoonotic pathogen that produces 146-kDa modular toxin (PMT), causes progressive atrophic rhinitis with severe turbinate bone degradation in pigs. However, its mechanism of cytotoxicity remains unclear. In this study, we expressed PMT, purified it prokaryotic expression system, and found killed PK15 cells. The host factor CXCL8 was significantly upregulated among the differentially genes transcriptome sequencing analysis qPCR verification. We constructed...
Different levels of EspP2 expression are seen in strains Glaesserella parasuis with high and low pathogenicity. As a potential virulence factor for G. parasuis, the pathogenic mechanism infection host cells is not clear. To begin to elucidate effect on virulence, we used SC1401 its wild-type form SC1401, which was made EspP2-deficient. We demonstrated that causes up-regulation claudin-1 occludin expression, thereby promoting adhesion cells; EspP2-deficiency resulted significantly reduced...
Although the Japanese encephalitis virus (JEV) infects various cell types, its receptor molecules are still not clearly understood. In our laboratory’s prior research, Rab4b was identified as a potential host factor that facilitates JEV infection in PK15 cells, utilizing genome-wide CRISPR/Cas9 knockout library (PK-15-GeCKO). To further explore effect of on replication, we used line using technology and overexpressing line, with IFA, RT–qPCR, Western blot to study viral replication whole...
Porcine epidemic diarrhea (PED) is an acute, highly contagious, and infectious disease caused by porcine virus (PEDV). PEDV can affect pigs of all ages, with 50~100% mortality in neonatal piglets substantial economic losses the swine industry. In present study, 347 fecal intestinal samples were collected from seven regions China during 2020–2022. A comprehensive molecular investigation spike (S) gene strains was carried out, which included phylogenetic analysis obtained sequences....
As a zoonotic virus, Japanese Encephalitis virus (JEV) poses serious threat to human health and the breeding industry. Regarding mechanism complications of tissue inflammation caused by JEV, such as encephalitis orchitis, there is no effective drug treatment currently, occurrence has not been thoroughly studied. Therefore, it necessary study inflammatory pathway JEV. one key proteins regulating cell death, BCL2 antagonist/killer (BAK) also prerequisite for release cellular factors. We found...
Diarrhea caused by enterotoxigenic Escherichia coli (ETEC) infections poses a significant challenge in global pig farming. To address this issue, the study was conducted to identify and characterize 19 ETEC isolates from fecal samples of diarrheic pigs sourced large-scale farms Sichuan Province, China. Whole-genome sequencing bioinformatic analysis were utilized for identification characterization. The exhibited substantial resistance cefotaxime, ceftriaxone, chloramphenicol, ciprofloxacin,...
Abstract Glaesserella parasuis is usually a benign swine commensal in the upper respiratory tract, but virulent strains can cause systemic infection characterized by pneumonia, meningitis, and fibrinous polyserositis. The intensive pulmonary inflammatory response following G. main of lung injury death pigs. Vaccination has failed to control disease due lack extended cross-protection. Accumulating evidence indicates that heme-binding protein A (HbpA) potential virulence determinant promising...
Glaesserella parasuis cytolethal distending toxin (GpCDT) can induce cell cycle arrest and apoptosis. Our laboratory’s previous work demonstrated that GTPase 4b (Rab4b) is a key host protein implicated in GpCDT-induced cytotoxicity. This study investigated the probable involvement of Rab4b process. used CRISPR/Cas9 technology to create Rab4b-knockout line. The results showed greater resistance In contrast, forced overexpression increased Further immunoprecipitation reveals GpCDT may bind...
Canine parvovirus (CPV) and distemper virus (CDV) can cause fatal diseases in giant panda (Ailuropoda melanoleuca). The main capsid protein of CPV VP2 be self-assembled to form virus-like particles (VLPs) vitro, which is great significance for potential vaccine development. In the present study, we remodeled a panda-derived CPV, where major CDV F N epitopes were incorporated N-terminal loop2 region two combinations chimeric VLPs. reactivity ability morphology recombinant proteins confirmed...
ABSTRACT Porcine deltacoronavirus (PDCoV) is a newly emerged enteric pathogen that causes diarrhea, dehydration, vomiting, and in suckling piglets, high rate of death. Here, we used endocytosis inhibitors, dominant-negative mutants, small interfering RNA (siRNA) interference to investigate PDCoV entry into swine testicle (ST) cells. Results showed chlorpromazine, dynasore, methyl-β-cyclodextrin (MβCD) inhibited internalization; immunofluorescence assay (IFA) (DN) mutants siRNA further...
Porcine circoviruses (PCVs) are notorious for triggering severe diseases in pigs and causing serious economic losses to the swine industry. In present study, we undertook a comprehensive approach investigation of PCV prevalence, including phylogenetic analysis obtained sequences, determination major circulating genotypes serological screening based on different recombinant Cap proteins with specific immunoreactivity. Epidemiological surveillance data indicate that PCV2d PCV3a widely...
Dental caries severely hinders efficient access to adequate energy in wildlife. Different food supplies will develop characteristic plaque, and the microorganisms of these plaque are closely related dental health. Here, samples from panda cubs with caries-free were collected for 16S rRNA high-throughput sequencing. All sequences clustered into 337 operational taxonomic units (OTUs; 97% identity), representing 268 independent species belonging 189 genera, 98 families, 51 orders, 24 classes,...
Glaesserella parasuis (G. parasuis.) is the etiological pathogen of Glässer’s disease, which causes high economic losses to pig industry. The heme-binding protein A precursor (HbpA) was a putative virulence-associated factor proposed be potential subunit vaccine candidate in G. parasuis. In this study, three monoclonal antibodies (mAb) 5D11, 2H81, and 4F2 against recombinant HbpA (rHbpA) SH0165 (serotype 5) were generated by fusing SP2/0-Ag14 murine myeloma cells spleen from BALB/c mice...
Transmissible gastroenteritis virus (TGEV) is an important swine enteric coronavirus causing viral diarrhea in pigs of all ages. Currently, the development antiviral agents targeting host proteins to combat infection has received great attention. The heat shock protein 90 (HSP90) a critical factor and regulatory effects on various viruses. However, its roles porcine remain unclear. In this study, effect HSP90 TGEV was evaluated. addition, influence inhibitor VER-82576 proinflammatory...
Trichosporon asahii is a basidiomycete yeast that pathogenic to humans and animals, fluconazole-resistant strains have recently increased. Farnesol secreted by fungi factor causes variations in fluconazole resistance; however, few studies explored the underlying mechanisms. Therefore, this study aims delineate resistance mechanisms of T. explore farnesol’s effects on these processes. A comparative metabolome–transcriptome analysis untreated fluconazole-sensitive (YAN), (PB) strains, 25 μM...
Currently, there are two means used in commercially available digital PCR systems. The first one uses microfluidic chambers or microwells to split the sample into hundreds of nanolitre volumes. Microfluidic chips simplify reaction setup; nevertheless, it is challenging scale achieve high-throughput. second based on emulsion PCR, called BEAMing. BEAMing requires specialized heterogeneous assay schemes that add complexity workflow, thereby limiting its adoption a few applications including DNA...