A5006673622- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Contact Dermatitis and Allergies
- Occupational exposure and asthma
- Transgenic Plants and Applications
- Gut microbiota and health
- Eosinophilic Esophagitis
- Asthma and respiratory diseases
- COVID-19 Clinical Research Studies
- Clostridium difficile and Clostridium perfringens research
- Advanced biosensing and bioanalysis techniques
- interferon and immune responses
- HIV/AIDS Research and Interventions
- Dermatology and Skin Diseases
- Agricultural pest management studies
- Immunodeficiency and Autoimmune Disorders
- Proteins in Food Systems
- Advanced Proteomics Techniques and Applications
- COVID-19 and healthcare impacts
- Plant Surface Properties and Treatments
- HIV, Drug Use, Sexual Risk
- Polysaccharides and Plant Cell Walls
- Soybean genetics and cultivation
- Liver Disease Diagnosis and Treatment
- Seed Germination and Physiology
Hospital Universitario Infanta Leonor
2015-2025
Instituto Ramón y Cajal de Investigación Sanitaria
2023-2024
Hospital Universitario Ramón y Cajal
2023-2024
Instituto de Investigación Biomédica de Málaga
2015-2024
Universidad de Alcalá
2023-2024
Instituto de Salud Carlos III
2021-2024
Universidad Complutense de Madrid
2015-2023
Centro de Investigación Biomédica en Red
2023
Hospital Universitario del Sureste
2023
Paul Ehrlich Institut
2021
MicroRNAs (miRNAs) have a crucial role in regulating immune response against infectious diseases, showing changes early disease onset and before the detection of pathogen. Thus, we aimed to analyze plasma miRNA profile at COVID-19 identify miRNAs as prognostic biomarkers severity survival.Plasma miRNome 96 patients that developed asymptomatic/mild, moderate severe was sequenced together with group healthy controls. Plasma immune-related were also assessed. showed 200 significant...
Despite effective antiretroviral therapy, people with HIV (PWH) experience persistent systemic inflammation and increased morbidity mortality. Modulating the gut microbiome through fecal microbiota transplantation (FMT) represents a novel therapeutic strategy. We aimed to evaluate proteomic changes in inflammatory pathways following repeated, low-dose FMT versus placebo. This double-masked, placebo-controlled pilot study assessed impacts of weekly placebo treatment over 8 weeks on 29 PWH...
Tomato has become one of the most consumed vegetables worldwide, but its intake is often accompanied by an increasing risk inducing allergic symptoms. The aim work was identification new seed-specific allergens associated with severe symptoms in patients to this edible vegetable.We used 22 sera from well-defined tomato-allergic patients. seed proteins were purified and analyzed for biochemical immunological characterization. A mixture two IgE-binding nsLTPs tomato seeds. Both allergens, Sola...
The COVID-19 pandemic and associated lockdown measures have been with substantial disruptions to health care services, including screening for human immunodeficiency virus (HIV) management of people living HIV (PLWH). Data from 3265 patients were examined in a retrospective cohort study. We compared outpatient follow-up PLWH, the number new patients, treatment adherence, hospitalizations, deaths during “pandemic period” (March 2020 February 2021), “pre-pandemic (the equivalent time frame...
Given the increased popularity of flaxseed in meals, several cases allergy to these seeds have been reported. Little is known about allergens implicated hypersensitivity reactions flaxseed. The present study aimed identify involved IgE-mediated 5 patients with a clinical history severe systemic symptoms after consumption.Proteins that were potential IgE-binding capacity purified from extract using chromatography and identified via MALDI-TOF mass spectrometry. Immunoassays performed allergic...
2S albumins are relevant and often major allergens from several tree nuts seeds, affecting mainly children young people. The present study aims to assess how the structural features of could affect their immunogenic capacity, which is essential comprehend role these proteins in food allergy. For this purpose, twelve were isolated respective extracts by chromatographic methods identified MALDI-TOF mass-spectrometry. Their molecular characterization was conducted electrophoretic, spectroscopic...
Peach tree (PT) pollen sensitization is highly prevalent in subjects living areas where this widely cultivated. None of the allergens responsible for these sensitizations have been identified so far. Our aim was to identify most relevant PT and analyze their capacity inducing respiratory symptoms. We studied sixty-two individuals sensitized who developed symptoms after its exposure. The IgE binding profile on peach extract by means immunoblotting using sera from analyzed. Protein...
Mustard seed allergen Sin a1 may activate mucosal barrier human intestinal epithelial cells (A) and/or dendritic (B), to initiate allergic sensitization by allowing consecutive type 2 activation of DC/T-cells, T/B-cells, mast cells.
The influence of gastrointestinal digestion on the immunological properties three different nonspecific lipid-transfer proteins (nsLTPs) described in tomato fruit has been assessed using an vitro system mimicking stomach and intestine conditions. Tomato peel/pulp nsLTP, Sola l 3, was degraded after digestion, although immunoglobulin E (IgE) recognition intact protein a 10 kDa band were still observed 30 min duodenal presence phosphatidylcholine. seed 7, showed higher stability than other...
Objectives Several studies have described peach tree (PT) as an occupational allergen. The aim of this work was to assess the effect Prunus persica 9 (Pru p 9), a recently identified allergen from PT pollen, in exposed workers. Methods study included people who reported respiratory symptoms after handling orchards during flowering period (Blanca village, Murcia region, south-east Spain). After completing detailed questionnaire, participants underwent skin prick test (SPT) and nasal...
Peach tree allergens are present in fruit, pollen, branches, and leaves, can induce systemic, respiratory, cutaneous, gastrointestinal symptoms. We studied the capacity of peach fruit/Pru p 1, Pru 3, 4, 7 pollen/Pru 9 for inducing symptoms following oral or respiratory exposure a large group subjects. included 716 adults (aged 21 to 83 y.o.) exposed pollen fruit intake study population. Participants completed questionnaire were skin tested with panel inhalant food allergens, including...
Allergy can start at early ages, with genetic and environmental factors contributing to its development.The study aimed describe the pattern of sensitisation allergy in children adolescents Spanish versus Moroccan ancestry but born same rural area Spain.Participants were (3-19 years) or descent, Blanca, Murcia (Spain). A detailed questionnaire was completed, skin prick tests performed assess reactions most prevalent pollen allergens (O. europaea, P. pratense, S. kali, C. arizonica,...
<title>Abstract</title> Background Despite effective antiretroviral therapy, people with HIV (PWH) experience persistent systemic inflammation, increasing morbidity and mortality. Modulating the gut microbiome through fecal microbiota transplant (FMT) represents a novel therapeutic strategy. We aimed to evaluate proteomic changes in inflammatory pathways following repeated, low-dose FMT versus placebo. Methods This double-masked, placebo-controlled pilot study assessed impacts of weekly...
Abstract Due to the benefits of tomato as an antioxidant and vitamin source, allergy this vegetable food is a clinically concerning problem. Sola l 7, class I lipid transfer protein found in seeds, has been identified allergen linked severe anaphylaxis. However, role binding 7‐induced remains unclear. Here, three‐dimensional structure recombinant 7 (rSola 7) elucidated using nuclear magnetic resonance spectroscopy (NMR). Its interaction with free fatty acids deeply studied; fluorescence...
Food allergy is an increasing health problem with many proteins involved that belong mainly to a limited number of families. They show high level cross-reactivity. In the Mediterranean area, most prevalent food allergens are those vegetal origin. Although tomato (Solanum lycopersium L.) one implicated foods, studies on identification and relevance their have not been carried out in detail. This could be particularly relevant for seeds as happen other fruits like kiwi. The aimed was analyse...