A5022980886- Venomous Animal Envenomation and Studies
- Rabies epidemiology and control
- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Structural Characterization
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Chronic Lymphocytic Leukemia Research
- Glycosylation and Glycoproteins Research
- Blood groups and transfusion
- Mosquito-borne diseases and control
- Immunodeficiency and Autoimmune Disorders
- Antimicrobial Peptides and Activities
- Dental Research and COVID-19
- Healthcare and Venom Research
- Cytokine Signaling Pathways and Interactions
- Marine Invertebrate Physiology and Ecology
- Advanced biosensing and bioanalysis techniques
- Diabetes Management and Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Diet and metabolism studies
- Chronic Myeloid Leukemia Treatments
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Gene Regulatory Network Analysis
- Immunotherapy and Immune Responses
Centre National de la Recherche Scientifique
2016-2024
Modélisation et Ingénierie des Systèmes Complexes Biologiques pour le Diagnostic
2016-2024
Université de Montpellier
2021-2022
Universidade Federal de Minas Gerais
2011-2016
Ezequiel Dias Foundation
2010-2013
West University of Timişoara
1997
Max Planck Institute of Biochemistry
1997
Heidelberg University
1995-1996
Abstract Covid-19 crisis showed us that rapid massive virus detection campaign is a key element in SARS-CoV-2 pandemic response. The classical RT-PCR laboratory platforms must be complemented with and simplified technologies to enhance efficiency of large testing strategies. To this aim, we developed EasyCOV, direct saliva RT-LAMP based assay do not requires any RNA extraction step. It allows robust response under safe easy conditions for healthcare workers patients. EasyCOV test was...
We report the isolation and structure–function relationship of a 23 kDa metalloproteinase named atroxlysin-I from venom Peruvian Bothrops atrox (Jergón). Atroxlysin is P-I contains 204 residues. Its proteolytic activity towards dimethylcasein enhanced by Ca+2 but inhibited EDTA, dithiothreitol, excessive Zn+2 α2-macroglobulin. Unlike other structurally homologous metalloproteinases, causes hemorrhages. To examine its hemorrhagic mechanistically, we studied function in vitro vivo. It cleaved...
We report the detailed molecular characterization of two PLA2s, Lys49 and Asp49 isolated from Bothrops leucurus venom, examined their effects against Dengue virus (DENV). The Bl-PLA2s, named BlK-PLA2 BlD-PLA2, are composed 121 122 amino acids determined by automated sequencing native proteins peptides produced digestion with trypsin. They contain fourteen cysteines pIs 9.05 8.18 for BlK- BlD-PLA2s, show a high degree sequence similarity to homologous snake venom but may display different...
Epitope identification is essential for developing effective antibodies that can detect and neutralize bioactive proteins. Computational prediction a valuable time-saving alternative experimental identification. Current computational methods epitope are underused undervalued due to their high false positive rate. In this work, we targeted common properties of linear B-cell epitopes identified in an individual protein class (metalloendopeptidases) introduced method reduce the rate increase...
Abstract In the context of social events reopening and economic relaunch, sanitary surveillance SARS-CoV-2 infection is still required. Here, we evaluated diagnostic performances a rapid, extraction-free connected reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay on saliva. Nasopharyngeal (NP) swabs saliva from 443 outpatients were collected simultaneously tested by quantitative PCR (RT-qPCR) as reference standard test. Seventy-one individuals (16.0%) positive NP...
The main goal of this work was to develop a strategy identify B-cell epitopes on four different three finger toxins (3FTX) and one phospholipase A2 (PLA2) from Micrurus corallinus snake venom. 3FTx PLA2 are highly abundant components in Elapidic venoms the major responsibles for toxicity observed envenomation by coral snakes. Overlapping peptides sequence each toxin were prepared SPOT method anti-elapidic sera used map epitopes. After immunogenicity analysis spot-reactive EPITOPIA,...
The main purpose of this study is to investigate the in vitro toxic effects 5 Bothrops spp. snake venoms, which are part antigenic mixture used for production Brazilian antivenom, and evaluate their correlation with vivo activities venoms. analysis could be helpful replacement living animals experimentation bioassay. Cytotoxicity, L-amino acid oxidase (LAAO), proteolitic (serine metalloproteinase), hyaluronidase (Hyal), phospholipase A2 (PLA2) were estimated coefficient was determined each...
This work used eleven Peruvian snake venoms (Bothrops andianus, Bothrops atrox, barnetti, castelnaudi, Bothriopsis chloromelas, Bothrocophias microphthalmus, neuwiedi, oligolepis, peruviana, pictus and taeniata) to perform in vitro experimentation determine its main characteristics. Hyaluronidase (HYAL), phospholipase A2 (PLA2), venom metalloproteinase (SVMP), serine protease (SVSP) l-amino acid oxidase (LAAO) activities; toxicity by cell viability assays using MGSO3, VERO HeLa lineages;...
Background The snake Bothrops atrox is responsible for the majority of envenomings in northern region South America. Severe local effects, including hemorrhage, which are mainly caused by venom metalloproteinases (SVMPs), not fully neutralized conventional serum therapy. Little known about immunochemistry P-I SVMPs since few monoclonal antibodies (mAbs) against these molecules have been obtained. In addition, producing toxin-neutralizing mAbs remains very challenging. Methodology/Principal...
Mutalysin-II (mut-II) from Lachesis muta snake venom is an endopeptidase with hemorrhagic activity. A mAb against mutalysin-II that neutralized the effect was produced previously. To identify epitopes, sets of 15-mer overlapping peptides covering mut-II amino acid sequence were synthesized using SPOT method and tested but failed to react mAb. Using a phage-display approach seventeen clones reactive identified. Additional immunoassays identified QCTMDQGRLRCR, TCATDQGRLRCT, HCFHDQGRVRCA,...
Atroxlysin-I (Atr-I) is a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops atrox venom, the responsible for majority of bites in north region South America. SVMPs like Atr-I produce toxic effects victims including hemorrhage, inflammation, necrosis and blood coagulation deficiency. Mapping B-cell epitopes might result identification non-toxic molecules capable inducing neutralizing antibodies improving anti-venom therapy. Here, using SPOT-synthesis technique we identified two...
Abstract Natural anti-IgG autoantibodies are found both in healthy individuals and patients with certain diseases. One group of these Abs recognizes epitopes located the F(ab')2 region IgG molecule. The immunoregulatory role individuals, graft rejection, disease was previously studied, usually a focus on characterization anti-idiotypic Abs. In present study, we characterize epitope recognized by an anti-F(ab')2gamma autoantibody IgA isotype, which occurs serum kidney transplant recipients....
Antibodies not only play a major role in clinical diagnostics and biopharmaceutical analysis but also are class of drugs that regularly used to treat numerous diseases. The identification antibody–epitope binding sites is then great interest many emerging medical bioanalytical applications, particularly design monoclonal antibodies (mAb) mimics taking advantage amino acid residues involved the binding. Among relevant antibodies, antibody rituximab has received significant attention as it...
Bothrops andianus is a venomous snake found in the area of Machu Picchu (Peru). Its venom not included antigenic pool used for production Peruvian anti-bothropic anti-venom. B. can elicit many biological effects such as hemorrhage, hemolysis, proteolytic activity and lethality. The anti-venom displays consistent cross-reactivity with venom, by ELISA Western Blotting also effective neutralizing venom's toxic activities.
Background: Response to the COVID-19 outbreak calls for rapid and accurate diagnostic tests control pandemic outbreak. We aimed evaluate saliva specimen as well RT-LAMP test diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.Methods: This monocentric study screened outpatients that were either symptomatic or contact-cases a confirmed case.Nasopharyngeal-swabs (NPS) collected simultaneously tested by RT-qPCR. Saliva samples also analyzed using an...
Bothrops species are the main cause of snake bites in rural communities tropical developing countries Central and South America. Envenomation by snakes is characterized prominent local inflammation, hemorrhage necrosis as well systemic hemostatic disturbances. These pathological effects mainly caused major toxins viperidae venoms, venom metalloproteinases (SVMPs). Despite antivenom therapy efficiency to block toxic on bite victims, this treatment shows limited efficacy prevent tissue...
Current evolutions in medical practices induce a change of paradigm with the convergence diagnosis and therapy, going to precision medicine “theranostics”. One can observe new role biomarkers biomedical therapeutic applications, for instance development molecular multiplex biosensors (nucleic acid, proteins, metabolites). In addition, there is an increasing interest point-of-care (POC) home monitoring/testing technologies devoted probe patient parameters his direct environment. The obvious...
Background: Insulin resistance (IR) is a risk factor for type 2 diabetes (T2D) and cardiovascular diseases (CVD). Early identification of IR in large populations remains uneasy, although desirable promoting lifestyle modifications T2D/CVD prevention. Fasting blood branched-chain amino acids (bBCAA) are biomarkers their impaired catabolism contributes to development. Methods: We present here an innovative test fast reliable screening based on the detection urine BCAA (uBCAA). In this...