A5070549313- Clostridium difficile and Clostridium perfringens research
- Microscopic Colitis
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Mycobacterium research and diagnosis
- Nosocomial Infections in ICU
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Cardiac, Anesthesia and Surgical Outcomes
- Helicobacter pylori-related gastroenterology studies
- Cystic Fibrosis Research Advances
- Gastrointestinal motility and disorders
- COVID-19 and healthcare impacts
- DNA Repair Mechanisms
- Inflammatory Bowel Disease
- Liver Diseases and Immunity
- Ultrasound in Clinical Applications
- Medical Device Sterilization and Disinfection
- Bacteriophages and microbial interactions
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pediatric health and respiratory diseases
- Respiratory Support and Mechanisms
- Pharmacological Effects of Natural Compounds
- Geriatric Care and Nursing Homes
- Global Health and Surgery
University of Birmingham
2005-2025
The Edgbaston Hospital
2024
Heartlands Hospital
2013-2021
Heart of England NHS Foundation Trust
2011-2018
Public Health England
2018
Public Health Agency
2016
Birmingham City Hospital
1999
Primary sclerosing cholangitis (PSC) is the classical hepatobiliary manifestation of inflammatory bowel disease (IBD). The strong association between gut and liver inflammation has driven several pathogenic hypotheses to which intestinal microbiome proposed contribute. Pilot studies faecal microbiota transplantation (FMT) in PSC IBD are demonstrated be safe associated with increased bacterial diversity. However, longevity such changes impact on markers activity progression have not been...
ABSTRACT Clostridium difficile infection is almost unrecognized in mainland China. We have undertaken a study large Chinese teaching hospital Changsha, Hunan, China, to identify cases of C. , record patient characteristics, and define the molecular epidemiology with respect ribotype distribution cross-infection. Between April 2009 February 2010, we examined fecal samples from 70 hospitalized patients diarrhea who were receiving or had received antibiotics within previous 6 weeks. Clinical...
The first British Society of Gastroenterology (BSG) and Healthcare Infection (HIS)-endorsed faecal microbiota transplant (FMT) guidelines were published in 2018. Over the past 5 years, there has been considerable growth evidence base (including publication outcomes from large national FMT registries), necessitating an updated critical review literature a second edition BSG/HIS guidelines. These have produced accordance with National Institute for Health Care Excellence-accredited...
ESBL-producing Enterobacteriaceae (ESBLPE) are increasing in prevalence worldwide and more difficult to treat than non-ESBLPE. Their the UK general population is unknown, as only previous ESBLPE faecal colonization study involved patients with diarrhoea.To estimate of CTX-M adult England 2014, investigate risk factors.A stratified random sample 58 337 registered from 16 practices within four areas were invited participate by returning faeces specimens self-completed questionnaires. Specimens...
Glutaraldehyde-resistant Mycobacterium chelonae have been isolated from endoscope washer disinfectors and rinse water. The mechanism of glutaraldehyde resistance is not well understood. Two spontaneous, glutaraldehyde-resistant mutants the sensitive type strain, NCTC 946, were investigated. colony morphology two differed that strain: colonies former dry waxy whereas those latter smooth shiny. Increased to was matched by small increases in MICs rifampicin ethambutol but isoniazid. Both showed...
Faecal Microbiota Transplant (FMT) has improved outcomes for the treatment of Clostridioides difficile infection (CDI) compared to antibiotic therapy. FMT is classified as a medicinal product in United Kingdom, similar USA and Canada, limiting supply via stool banks without appropriate licencing. In largest UK cohort date, we describe clinical 124 patients receiving recurrent or refractory CDI present framework produce licenced product.Anonymous unrelated healthy donors, screened health...
PCR ribotyping is currently used in many countries for epidemiological investigation to track transmission and identify emerging variants of Clostridium difficile. Although differentiates over 300 types, it not always sufficiently discriminatory investigations particularly common ribotypes, e.g., ribotypes 027, 106, 017. Multilocus variable-number tandem-repeat analysis (MLVA) a highly molecular subtyping method that has been applied number bacterial species high-level subtyping. Two MLVA...
The Liverpool epidemic strain (LES) is an important transmissible clonal lineage of Pseudomonas aeruginosa that chronically infects the lungs people with cystic fibrosis (CF). Previous studies have focused on genomics LES in a limited number isolates, mostly from one CF centre UK, and highlighting identification Canada. Here we significantly extend current genome database by sequencing 91 isolates multiple centres across describe comparative this large collection UK Phylogenetic analysis...
Background Ulcerative colitis is an autoimmune reaction to imbalanced colonic microbiome in genetically predisposed patients. There have been four published randomised controlled trials of faecal microbiota transplant versus placebo ulcerative colitis; three being positive. However, the methodology used varied and mechanistic data were sparse. Objective To identify optimal delivery route test a future efficacy-powered trial. Design Prospective, multicentre, open-label, parallel-group, pilot...
The early identification of outbreaks is crucial for the control Clostridium difficile infection. This study aimed to determine if number hospital-acquired C. infections could be reduced by rapidly typing strains using multiple-locus variable-number tandem-repeat analysis (MLVA) compared PCR ribotyping. A total 16 hospitals were recruited study, and all periods increased incidence (PIIs) infection identified. randomized into two arms, test control, with isolates typed in MLVA Following a...
Summary A network of circuits, with KorB and TrbA as key regulators, controls genes for conjugative transfer broad host range plasmid RK2. To assess the importance regulon, mutational analysis was applied to operator at trbB promoter then other TrbA‐regulated promoters in tra region. All identified operators are submaximal; case trbBp , a G transition that made core perfect palindrome increased repression by about 50% compared wild type. When this change introduced into RK2 genome, decreases...
Background: Faecal transplantation is an evidence-based treatment for Clostridioides difficile. Patients infected with SARS-CoV-2 have been shown to shed the virus in stool up 33 days, well beyond average clearance time upper respiratory tract shedding. We carried out analytical and clinical validation of reverse-transcriptase quantitative (RT-qPCR) as LAMP, LamPORE droplet digital PCR detection RNA from donated samples faecal microbiota (FMT), spiked asymptomatic inpatients acute surgical...
Abstract Background Studies of faecal microbiota transplantation (FMT) for treating ulcerative colitis (UC) have shown promising results. Mechanisms by which FMT modulates inflammation, however, remain unexplored. Through a prospective, open-label pilot in UC (STOP-Colitis) we conducted sub-study to explore changes host colonic mucosal immune cell subsets and gene expression following FMT. Methods Patients this study received eight infusions over an 8-week period. Colon biopsies blood were...
Faecal microbiota transplantation (FMT) is a potentially life-saving treatment for patients with refractory and recurrent Clostridioides difficile infection cure rates of 90%.1Mullish BH Quraishi MN Segal JP et al.The use faecal transplant as or Clostridium other potential indications: joint British Society Gastroenterology (BSG) Healthcare Infection (HIS) guidelines.Gut. 2018; 67: 1920-1941Crossref PubMed Scopus (194) Google Scholar Substantial implications regard to the safe provision FMT...
Objectives This study aims to highlight problems with recruiting an English stool sample community prevalence study. It was part of a larger cross-sectional research determine the risk factors for presence extended-spectrum beta-lactamase and carbapenemase-producing coliforms in samples asymptomatic general population. Setting Four National Health Service primary care trusts (PCTs) England representing different section population England: Newham PCT; Heart Birmingham Teaching Shropshire...
<h3>Background</h3> <i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that chronically colonises the lungs of bronchiectasis patients and associated with a decline in lung function. Epidemic strains <i>P. Aeruginosa</i> including Liverpool, Manchester Midlands-1, have been described cystic fibrosis (CF) increased morbidity mortality, but not previously broncheiectasis patients. This study aimed to establish if there was cross transmission aeruginosa </i>amongst bronciectaisis...
ABSTRACT Introduction Faecal transplantation is an evidence based treatment for Clostridiodes difficile . Patients infected with SARS-CoV-2 have been shown to shed the virus in stool up 33 days, well beyond average clearance time upper respiratory tract shedding. We carried out analytical and clinical validation of reverse-transcriptase quantitative (RT-qPCR) as LAMP, LamPORE droplet digital PCR detection RNA from donated samples FMT, spiked asymptomatic inpatients acute surgical unit....
Fecal microbiota transplantation involves the infusion of intestinal microorganisms via transfer a stool from healthy individual into diseased individual, with intent restoring normal flora. transplant is proposed for treatment refractory Clostridioides difficile infection. At present, recurrent infection only indication supported by solid scientific evidence. Regulations healthcare authorities vary among different countries. Considering that Romania does not have an available national...