A5064202687- Cell Image Analysis Techniques
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Molecular Biology Techniques and Applications
- Ion channel regulation and function
- Advanced Proteomics Techniques and Applications
- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Brain Tumor Detection and Classification
- Radiomics and Machine Learning in Medical Imaging
- Biochemical and Structural Characterization
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Respiratory viral infections research
- Virus-based gene therapy research
- S100 Proteins and Annexins
- RNA Interference and Gene Delivery
- interferon and immune responses
- Microbial Inactivation Methods
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Trace Elements in Health
Duke University Hospital
2023-2024
Duke Medical Center
2023-2024
Guilin Medical University
2021-2024
Imperial College London
2024
Duke University
2020-2024
Chinese Academy of Agricultural Sciences
2024
Qingdao Agricultural University
2024
Institute of Animal Sciences
2024
Second Affiliated Hospital of Jilin University
2023
Shanghai Jiao Tong University
2021-2023
Astrocytes strongly promote the formation and maturation of synapses by secreted proteins. Several astrocyte-secreted synaptogenic proteins controlling excitatory synapse development were identified; however, those that induce inhibitory synaptogenesis remain elusive. Here, we identify neurocan as an protein. After secretion from astrocytes, is cleaved into N- C-terminal fragments. We found these fragments have distinct localizations in extracellular matrix. The fragment localizes to...
In photoreceptor synaptic terminals, voltage-gated Cav1.4 channels mediate Ca2+ signals required for transmission of visual stimuli. Like other high voltage-activated Cav channels, are composed a main pore-forming α1 subunit and auxiliary β α2δ subunits. Of the four distinct classes α2δ, β2 α2δ4 thought to co-assemble with subunits in photoreceptors. However, an understanding functional properties this combination is lacking. Here, we provide evidence that α1, β2, contribute channel...
SUMMARY Astrocytes strongly promote the formation and maturation of synapses by secreted proteins. To date, several astrocyte-secreted synaptogenic proteins controlling different stages excitatory synapse development have been identified. However, identities astrocytic signals that induce inhibitory remain elusive. Here, through a combination in vitro vivo experiments, we identified Neurocan as an protein. is chondroitin sulfate proteoglycan best known protein localized to perineuronal nets....
To confirm the specificity of antibody used to detect phosphorylated ERM (pERM) in immunostaining by using Lambda protein phosphatase treatment on brain sections from both wild-type (WT) and LRRK2 G2019Ski/mice. dephosphorylates proteins, which should eliminate phospho-ERM staining if is specific form ERM.
Voltage-gated Cav1.2 and Cav1.3 (L-type) Ca2+ channels regulate neuronal excitability, synaptic plasticity, learning memory. Densin-180 (densin) is an excitatory protein that promotes Ca2+-dependent facilitation of voltage-gated in transfected cells. Mice lacking densin (densin KO) exhibit defects spatial memory, increased anxiety-related behaviors-phenotypes more closely match those mice than Cav1.3. Therefore, we investigated the functional impact on Cav1.2. We report essential regulator...
As a critical technique for dissection of synaptic and cellular mechanisms, whole-cell patch-clamp recording has become feasible in vivo preparations including both anaesthetized awake mammalian brains. However, compared with vitro recording, often suffers from low success rates high access resistance, preventing its wide application physiological analysis neural circuits. Here, we describe experimental procedures achieving amphotericin B-perforated as well conventional (breakthrough) rats...
Cancerous Inhibitor of PP2A (CIP2A), an endogenous inhibitor, is upregulated and causes reactive astrogliosis, synaptic degeneration, cognitive deficits in Alzheimer's disease (AD). However, the mechanism underlying increased CIP2A expression AD brains remains unclear. We here demonstrated that DNA damage-related Checkpoint kinase 1 (ChK1) activated human 3xTg-AD mice. ChK1-mediated overexpression drives inhibition activates STAT3, then leads to astrogliosis neurodegeneration vitro....
Abstract Synaptogenesis is essential for circuit development; however, it unknown whether critical the establishment and performance of goal-directed voluntary behaviors. Here, we show that operant conditioning via lever-press food reward training in mice induces excitatory synapse formation onto a subset anterior cingulate cortex neurons projecting to dorsomedial striatum (ACC →DMS ). Training-induced synaptogenesis controlled by Gabapentin/Thrombospondin receptor α2δ−1, which an neuronal...
Abstract During development, controlled synaptogenesis is required to form functioning neural circuits that underlie cognition and behavior. Astrocytes, a major glial-cell type in the central nervous system (CNS), promote synapse formation by secreting synaptogenic proteins. Thrombospondins 1 2 (TSP1/2), which act through their neuronal receptor α2δ-1, are for proper intracortical excitatory synaptogenesis. In adult brain, loss of α2δ-1 impairs training-induced anterior cingulate cortex...
Gentamicin (GM) is a commonly used antibiotic, and ototoxicity one of its side effects. Puerarin (PU) an isoflavone in kudzu roots that exerts number pharmacological effects, including antioxidative free radical scavenging The present study investigated whether PU could protect against GM‑induced C57BL/6J mice House Ear Institute‑Organ Corti 1 (HEI‑OC1) cells. HEI‑OC1 cells were to establish models this study. Auditory brainstem responses measured assess hearing thresholds, microscopy was...
Stereopsis is the ability to perceive depth using slightly different views from two eyes. This study aims conduct innovative stereopsis tests objective data outputted by eye tracking technology.A laptop and an tracker were used establish test system. Anaglyphic glasses employed execute assessment. The symbol was devised emulate quantitative measurement component of Random Dot 3 Stereo Acuity Test. Sub-pixel technology increase disparity accuracy pages. tested disparities were: 160″, 100″,...
Oxidative stress causes gut dysfunction and is a contributing factor in several intestinal disorders. Intestinal epithelial cell survival essential for maintaining human animal health under oxidative stress. 18beta-Glycyrrhetinic acid (GA) known to have multiple beneficial effects, including antioxidant activity; however, the underlying molecular mechanisms not been well established. Thus, present study evaluated therapeutic effects of GA on H2O2-induced porcine cells. The results showed...
Automatic lung organ segmentation on CT images is crucial for disease diagnosis. However, the unlimited voxel values and class imbalance of organs can lead to false-negative/positive leakage issues in advanced methods. Additionally, some slender are easily lost during recycled down/up-sample procedure, e.g., bronchioles & arterioles, causing severe discontinuity issue. Inspired by these, this paper introduces an effective method called Fuzzy Attention-based Border Rendering (FABR) network....
Rat astrocyte isolation and culture
Primary cortical neuron isolation and culture