A5048199443- Ubiquitin and proteasome pathways
- interferon and immune responses
- HIV Research and Treatment
- Cytomegalovirus and herpesvirus research
- Protein Degradation and Inhibitors
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Infant Development and Preterm Care
- Respiratory viral infections research
- Immune cells in cancer
- Peptidase Inhibition and Analysis
- HIV/AIDS drug development and treatment
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Protein Kinase Regulation and GTPase Signaling
- Inhalation and Respiratory Drug Delivery
- Innovative Energy Harvesting Technologies
- Pediatric health and respiratory diseases
- Statistical Methods in Clinical Trials
- Protein Tyrosine Phosphatases
- Bauxite Residue and Utilization
- Cancer-related molecular mechanisms research
- NF-κB Signaling Pathways
- Maternal Mental Health During Pregnancy and Postpartum
First Hospital of Jilin University
2013-2025
Jilin University
2013-2025
Institute of Virology of the Slovak Academy of Sciences
2024
Shanghai Jiao Tong University
2022-2024
Cangzhou Central Hospital
2023
Nantong University
2023
Shenzhen Third People’s Hospital
2022
National Clinical Research Center for Digestive Diseases
2022
Guangzhou University of Chinese Medicine
2021-2022
Jiangxi Maternal and Child Health Hospital
2022
Abstract The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has a global health crisis; its structural protein envelope (E) is critical for viral entry, budding, production, and induction of pathology which makes it potential target therapeutics against COVID-19. Here, we find that the E3 ligase RNF5 interacts with catalyzes ubiquitination E on 63rd lysine, leading to degradation ubiquitin-proteasome system (UPS). Importantly,...
The cellular biological function of the ubiquitin-proteasome pathway as an important modulator for regulation many fundamental processes has been greatly appreciated. critical role in viral pathogenesis become increasingly apparent. It is a powerful tool that host cells use to defend against infection. Some proteins can restriction factors limit infection by ubiquitin-dependent degradation. In this research, we identificated CUL4B-DDB1-PRPF19 E3 Ubiquitin Ligase Complex mediate proteasomal...
ABSTRACT Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) open reading frame 9b (ORF9b) antagonizes the antiviral type I and III interferon (IFN) responses is ubiquitinated degraded via ubiquitin-proteasome pathway. However, E3 ubiquitin ligases that mediate polyubiquitination degradation of ORF9b remain unknown. In this study, we identified 14 specifically bind to SARS-CoV-2 ORF9b. Specifically, three ligases, HECT, UBA, WWE domain-containing protein ligase 1 (HUWE1), component...
Coronavirus disease 2019 (COVID-19) is a current global health threat caused by SARS-CoV-2. The innate immune response such as type I IFN (IFN-I) the first line of host defense against viral infections, whereas SARS-CoV-2 proteins antagonize IFN-I production through distinct mechanisms.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for disease 2019 (COVID-19), remains a global public health threat with major economic implications. The non-structural protein 16 (nsp16) of SARS-CoV-2, in complex nsp10, catalyses final step viral mRNA capping via its 2’-O-methylase activity, enabling to evade host immunity and protect from degradation. However, factors regulating nsp16 have not been thoroughly explored. Although various E3 ubiquitin...
<title>Abstract</title> Objective: Investigate patients' clinical characteristics and bronchoscopic features with multidrug-resistant tracheobronchial tuberculosis. Methods: A total of 118 patients confirmed diagnosis tuberculosis were selected retrospectively, the demographic data, characteristics, manifestations analyzed. Results: Among tuberculosis, main cough (92.3%,109/118) sputum (83.0%,98/118). The primary infection sites right upper bronchus (39.8%,47/118) left (37.3%,44/118). types...
Interferon-inducible protein IFI204 (p204, the murine homolog of human IFI16) is known as a cytosolic DNA sensor to recognize viruses and intracellular bacteria. However, little about its role during extracellular bacterial infection. Here we show that required for host defense against infection Staphylococcus aureus, an pathogen. deficiency results in decreased survival, increased loads, severe organs damage, recruitment neutrophils macrophages. Production several inflammatory...
The SARS-CoV-2 envelope (E) protein is highly conserved among different viral variants and important for assembly production. Our recent study found that the E ubiquitinated degraded by E3 ligase RNF5 through proteasome pathway. However, whether ubiquitination can be reversed host deubiquitinase has not yet been determined. Here, we identify mass spectrum analysis deubiquitinases USP14 USP39 specifically interact with E, while potently reverses polyubiquitination. interacts via arginine-rich...
The lentiviral accessory proteins Vpx and Vpr are known to utilize CRL4 (DCAF1) E3 ligase induce the degradation of host restriction factor SAMHD1 or helicase transcription (HLTF), respectively. Selective disruption viral could be a promising antiviral strategy. Recently, we have determined that posttranslational modification (neddylation) Cullin-4 is required for activation Vpx-CRL4 ligase. However, mechanism Vpx/Vpr-CRL4 assembly still poorly understood. Here, report zinc coordination an...
Recently, triboelectric nanogenerators (TENGs) have made significant progress in energy harvesting and monitoring of human motion. In this work, we design a new wave structure nanogenerator (WS-TENG) for mechanical harvesters self-powered sensors. The WS-TENG consist three parts: top plate, middle layer bottom plate. By studying the with different shapes, designed optimal shape layer. maximum output power can reach 400.2 µW load 10 MΩ. generate electricity curved state, feature be used to...
SARS-CoV-2 belongs to the subgenus
Enterovirus 71 (EV71) is the causative pathogen of hand, foot, and mouth disease (HFMD). However, no effective antiviral therapy currently available. Some viruses could escape host’s innate immunity by upregulating suppressor cytokine signaling (SOCS) proteins. Until now, whether EV71 evades host immune system regulating expression SOCS proteins remains unknown. In this study, we found that infection promoted at both mRNA protein levels in vitro vivo. Consistently, infectivity was decreased...
Ubiquitination plays an important role in human immunodeficiency virus 1 (HIV-1) infection. HIV proteins such as Vif and Vpx mediate the degradation of host APOBEC3 SAMHD1, respectively, through proteasome pathway. However, whether deubiquitylating enzymes play essential HIV-1 infection is largely unknown. Here, we demonstrate that deubiquitinase USP21 potently inhibits production by indirectly downregulating expression transactivator transcription (Tat), which for transcriptional elongation...
Objective Developing a non-invasive and reliable triage test for endometrial malignant lesions is an important goal, as it could help to reduce the number of invasive diagnostic procedures required improve patient survival. We aimed estimate value DNA methylation levels in cervical cytological samples cancer (EC) atypical hyperplasia (AH). Methods A total 607 women who had indications biopsy Department Obstetrics Gynecology Cangzhou Central Hospital from October 2022 April 2023 were enrolled...
In this work, related performances of asphalt binders with Bayer red mud powder (RMP) were studied. RMP replaced the traditional limestone (LSP) as a filler in binder. The replacement rates 0%, 25%, 50%, 75%, and 100%, respectively. study, seven F/A (filler-to-asphalt, weight/weight) ratios for each fillers selected: 0.3, 0.6, 0.9, 1.2, 1.5, 1.8, 2.1. Penetration, softening point, rotational viscosity (RV), dynamic shear rheometry (DSR), bending beam (BBR) tests used to evaluate properties...
Psoralen (PSO) exerts anti‑inflammatory pharmacological effects and plays an important role in a variety of inflammatory diseases. However, the PSO with allergic rhinitis (AR) are yet to be reported. In present study, vitro AR model was generated by inducing JME/CF15 human nasal epithelial cells IL‑13, after which MTT used assess cytotoxicity PSO. The expression levels cytokines (granulocyte‑macrophage colony‑stimulating factor Eotaxin) were determined ELISA. Furthermore, IL‑6 ‑8, as well...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and its variants have caused hundreds of thousands deaths shown serious social influence worldwide. Jilin Province, China, experienced the first wave outbreak from December 2020 to February 2021. Here, we analyzed genomic characteristics SARS‐CoV‐2 in province using a phylogeographic tree found that clinical isolates belonged B.1 lineage, which was considered be ancestral lineage. Several dominant specific linear B cell...
Hijacking host ubiquitin pathways is essential for the replication of diverse viruses. However, role deubiquitinating enzymes (DUBs) in interplay between viruses and poorly characterized. Here, we demonstrate that specific DUBs are potent inhibitors viral proteins from HIVs/simian immunodeficiency (SIVs) involved evasion restriction factors replication. In particular, discovered T cell-functioning ubiquitin-specific protease 8 (USP8) a inhibitor HIV-1 virion infectivity factor (Vif)-mediated...
Verification of new reagent lots is a part the crucial tasks in clinical laboratories. The Clinical and Laboratory Standards Institute (CLSI) EP26-A guideline provides laboratories with an evaluation method for verification. purpose this study was to compare performance our laboratory lot verification protocol get final scheme.16 chemiluminescence analytes including estradiol (E2), progesterone (P), ferritin (FER), cortisol (COR),carbohydrate antigen 153 (CA153), free prostate-specific...