A5101586795- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Immune cells in cancer
- Histone Deacetylase Inhibitors Research
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Retinal Diseases and Treatments
- Cancer, Hypoxia, and Metabolism
- Ophthalmology and Visual Impairment Studies
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Intraocular Surgery and Lenses
- Cancer Research and Treatments
- Autophagy in Disease and Therapy
- Corneal surgery and disorders
- Trace Elements in Health
- Plant Stress Responses and Tolerance
- Cancer Immunotherapy and Biomarkers
- Toxoplasma gondii Research Studies
- Kruppel-like factors research
- Cancer, Stress, Anesthesia, and Immune Response
- Medical Imaging Techniques and Applications
Second Affiliated Hospital of Zhejiang University
2021-2025
Zhejiang University
2019-2025
Soochow University
2024-2025
Zhejiang Normal University
2024
Shanghai Polytechnic University
2022
Xinqiao Hospital
2015-2020
Duke University
2020
Army Medical University
2015-2020
Nanjing University of Chinese Medicine
2020
Nanjing Agricultural University
2020
Abstract Enhancer of zeste homolog (EZH2) is a key epigenetic regulator gene expression and frequently overexpressed in various cancer types, suggesting role oncogenesis. The therapeutic potential EZH2 inhibitors currently being explored, but their effect on antitumor immunity largely unknown. Here we report that suppressing activity using inhibitor GSK126 resulted increased numbers myeloid-derived suppressor cells (MDSC) fewer CD4+ IFNγ+CD8+ T cells, which are involved immunity. Addition...
Abstract Cancer is one of the most fatal diseases for decades. Aggregation‐induced emission luminogens (AIEgens) have been recently used as molecular imaging or therapeutic agents in cancers, due to advantages large Stokes shift, high quantum yield, great biocompatibility, and strong photostability. AIEgens can specifically target different types cancer via diverse targeting strategies. AIEgen‐based fluorescence imaging, especially near‐infrared demonstrated deep penetration suitable...
Enhancer of zeste homolog 2 (EZH2)-mediated trimethylation histone 3 lysine 27 (H3K27Me3) is critical for immune regulation. However, evidence lacking to address the effect EZH2 enzyme's activity on intestinal responses during inflammatory bowel disease (IBD). Here we report that suppressing ameliorates experimental inflammation and delayed onset colitis-associated cancer. In addition, identified an increased number functional MDSCs in colons, which are essential inhibitor activity....
Abstract Age-related cell loss underpins many senescence-associated diseases. Apoptosis of lens epithelial cells (LECs) is the important cellular basis senile cataract resulted from prolonged exposure to oxidative stress, although specific mechanisms remain elusive. Our data indicated concomitance high autophagy activity, low SQSTM1/p62 protein level and apoptosis in same LEC patients. Meanwhile, primary cultured LECs model, more durable activation obvious p62 degradation under stress were...
Excitotoxicity is a prevalent pathological event in neurodegenerative diseases. The involvement of ferroptosis the pathogenesis excitotoxicity remains elusive. Transcriptome analysis has revealed that cytoplasmic reduced nicotinamide adenine dinucleotide phosphate (NADPH) levels are associated with susceptibility to ferroptosis-inducing compounds. Here we show exogenous NADPH, besides being reductant, interacts N-myristoyltransferase 2 (NMT2) and upregulates N-myristoylated suppressor...
Cancer stem cells (CSCs, also called cancer stem-like cells, CSLCs) can function as "seed cells" for tumor recurrence and metastasis. Here, we report that, in the presence of CD133+ ovarian CSLCs, CD133- non-CSLCs undergo an epithelial-mesenchymal transition (EMT)-like process display enhanced metastatic capacity vitro vivo. Highly elevated expression chemokine (C-C motif) ligand 5 (CCL5) its receptors receptor (CCR) 1/3/5 are observed clinical murine tissues from epithelial carcinomas....
Lung cancer is the most commonly diagnosed and accounts for cancer-related mortalities worldwide. The high expression of programmed death ligand 1 (PD-L1) an important factor that promotes immune escape lung cancer, thus aggravates chemotherapy resistance poor prognosis. Therefore, understanding regulatory mechanism PD-L1 in critical tumor immunotherapy. Enhancer Zeste homolog2 (EZH2), epigenetic molecule with histone methyltransferase activity, formation immunosuppressive microenvironment....
Background: Pathologic myopia (PM) associated with myopic maculopathy (MM) and "Plus" lesions is a major cause of irreversible visual impairment worldwide. Therefore, we aimed to develop series deep learning algorithms artificial intelligence (AI)-models for automatic PM identification, MM classification, lesion detection based on retinal fundus images. Materials Methods: Consecutive 37,659 images from 32,419 patients were collected. After excluding 5,649 ungradable images, total dataset...
Gastric cancer (GC) is one of the most commonly occurring cancers, and metabolism-related genes (MRGs) are associated with its development. Transcriptome data relevant clinical were downloaded from The Cancer Genome Atlas Gene Expression Omnibus databases, we identified 194 MRGs differentially expressed between GC adjacent nontumor tissues. Through univariate Cox lasso regression analyses 13 potential prognostic (PDEMRGs). These PDEMRGs (CKMT2, ME1, GSTA2, ASAH1, GGT5, RDH12, NNMT, POLR1A,...
Parkinson's disease (PD) is a common neurodegenerative disorder worldwide, characterized by the loss of dopaminergic (DA) neurons in substantia nigra and associated with iron dyshomeostasis. Ferroptosis, form programmed cell death, involves iron-dependent lipid peroxidation serves as significant regulatory mechanism PD. This study identified Tp53-induced glycolysis apoptosis regulator (TIGAR) potential ferroptosis resistance PD development. In this study, we demonstrated that HT22 cells,...
Fluorescence-based microarrays are promising diagnostic tools due to their high throughput, small sample volume requirements, and multiplexing capabilities. However, low fluorescence output has limited implementation for in vitro diagnostics applications point-of-care (POC) settings. Here, by integration of a sandwich immunoassay microarray within plasmonic nanogap cavity, we demonstrate strongly enhanced which is critical readout inexpensive POC detectors. The consists inkjet-printed...
Mucins play a key role in tumorigenesis. MUC15 is membrane-bound mucin and the messenger RNA (mRNA) has been detected various organs. However, its tumor malignancy still unclear. This study was to investigate expression colorectal tumors of colon cancer cells. We found that mRNA significantly higher 70.8% (51/72) compared with their normal counterparts by real-time reverse transcription–polymerase chain reaction. Immunohistochemistry showed increased 82.6% (43/52) tumors. overexpression...
A novel hypothesis in cancer biology proposes that growth is driven by stem-like cells (CSLCs), also called tumor-initiating cells, which can self-renew and differentiate into multilineage progeny a fashion similar to stem cells. However, the impact underlying mechanisms of this process lung adenocarcinoma (LAC) remain be elucidated. Here, we report microRNA-214 (miR-214) contributes cell self-renewal directly targeting catenin beta interacting protein 1 (CTNNBIP1), member Wnt signaling...
Calcium (Ca2+) is a versatile intracellular second messenger that regulates several signaling pathways involved in growth, development, stress tolerance, and immune response plants. Autoinhibited Ca2+-ATPases (ACAs) play an important role the regulation of cellular Ca2+ homeostasis. Here, we systematically analyzed putative OsACA family members rice, according to phylogenetic tree OsACAs, OsACA9 was clustered into separated branch which its homologous gene Arabidopsis thaliana reported be...
To evaluate the influence of 2.2 mm clear corneal incision (CCI) features in surgically induced astigmatism (SIA) and higher-order aberrations (HOAs) after cataract surgery.Right eyes 92 subjects receiving surgery were involved. A total 38 categorized as intact group, 54 defective group. Pre- postoperative (1 month 6 months) HOAs on anterior posterior surfaces, volume, thickness (CT) measured using Pentacam. The CCI including length (IL), angles, distance from to central cornea (Dis-En/Ex),...
Abstract The lentivirus–short hairpin RNA (shRNA) system is a widely used tool for interference. Multiple factors may affect the interference efficiency during lentivirus production and transduction procedures. Thus, an optimized protocol required to achieve high‐titer efficient gene delivery. In present study, was produced by transfecting lentiviral transfer packaging plasmids into HEK 293T cells. affecting titer were assessed, including plasmid ratio, type, serum type cell culture,...
Epithelial-to-mesenchymal transition (EMT) is essential for the progression of non-invasive tumor cells into malignancy and metastasis. We found that miR-214 was increased in lung adenocarcinoma (LAD) positively associated with metastasis, which mediated by EMT. However, mechanism whereby overexpression microRNAs (miRNAs), such as miR-214, promote EMT LAD remains unclear. In this study, we TWIST1, an independent prognostic factor overall survival, correlated recurrence progression. also...
Abstract Pterygium is a common ocular disease with high recurrence rate, characterized by hyperplasia of subconjunctival fibrovascular tissue. Autophagy, an important process to maintain cellular homeostasis, participates in the pathogenic fibrosis different organs. However, exact role autophagy pterygium pathogenesis remains unknown. Here, we found that autophagic activity was decreased human tissues compared adjacent normal conjunctival tissues. The vitro model successfully established...
To evaluate changes in corneal higher-order aberrations (HOAs) on the anterior and posterior surfaces after 1.8 mm microincision cataract surgery (MICS) 2.8 small-incision (SICS).Eye Department, First Affiliated Hospital, School of Medicine, Zhejiang University, China.Prospective case series.Right eyes patients had MICS or SICS. The preoperative 1-week 3-month postoperative distance visual acuity (CDVA) dry eye-related indices were determined. total HOAs Zernike coefficients (3rd 4th order)...
This study aims to elucidate the role of Fe