A5046743695- Mosquito-borne diseases and control
- Drug Transport and Resistance Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Lung Cancer Treatments and Mutations
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Virus-based gene therapy research
- Cancer-related gene regulation
- Esophageal and GI Pathology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA and protein synthesis mechanisms
- Delphi Technique in Research
- Medicinal Plants and Bioactive Compounds
- Platelet Disorders and Treatments
- Nuclear Structure and Function
- Histone Deacetylase Inhibitors Research
- Pesticide and Herbicide Environmental Studies
- Environmental Toxicology and Ecotoxicology
- Cancer therapeutics and mechanisms
- HIV/AIDS drug development and treatment
- Pesticide Exposure and Toxicity
Auckland University of Technology
2019-2024
Tsinghua University
2024
Shanxi Medical University
2020
Taihe Hospital
2014
Fujian Entry - Exit Inspection and Quarantine Bureau
2011-2014
Nanjing Medical University
2009-2014
Hubei University of Medicine
2014
Shanghai Entry-Exit Inspection and Quarantine Bureau
2014
Government of Jiangsu Province
2014
Pyrethroid insecticides have been extensively used in China and worldwide for public health pest control. Accurate resistance monitoring is essential to guide the rational use of management. Here we examined nucleotide diversity para-sodium channel gene, which confers knockdown (kdr) Culex pipiens pallens mosquitoes China. The sequence analysis gene identified L1014F L1014S mutations. We developed validated allele-specific PCR real-time TaqMan methods diagnosis. method more superior as...
In recent years, attractive toxic sugar bait has been used in the mosquito control nature, and achieved good effects. However, current researches about did not focus on whether wild population for is resistant or not. The purpose of this study was to evaluate effectiveness against populations test effects mosquitoes with different levels resistance. Boric acid, dinotefuran deltamethrin were separately formulated into their anti-mosquito activity Culex quinquefasciatus. Using baits boric acid...
Our recent publications showed that multidrug resistance protein 2 (MRP2, encoded by the ABCC2 gene) conferred oxaliplatin in human liver cancer HepG2 cells. However, contribution of MRP2 to remains unclear colorectal and pancreatic lines. We investigated effects silencing siRNA on accumulation sensitivity Caco-2 cells PANC-1 characterized ATPase activities using membrane vesicles. Over-expression (endogenously cells) was associated with decreased cytotoxicity, but those deficits were...
To explore if trypsin could catalyze the degradation of non-protein molecule deltamethrin, we compared in vitro hydrolytic reactions deltamethrin presence and absence with ultraviolet-visible (UV/Vis) spectrophotometry gas chromatography-mass spectrometry (GC/MS). In addition, acute oral toxicity products was determined Wistar rats. The results show that absorption peak is around 264 nm, while peaks are 250 nm 296 nm. our GC setting, retention time undegraded 37.968 min, those were 15.289...
Horizontal gene transfer plays an essential role in evolution and ecological adaptation, yet this phenomenon has remained controversial, particularly where it occurs between prokaryotes eukaryotes. There are a handful of reported examples horizontal occurring eukaryotes the literature, with most these documented cases pertaining to invertebrates endosymbionts. However, vast majority horizontally transferred genes were either eventually excluded or rapidly became nonfunctional recipient...
Gemcitabine‑based chemotherapy has been widely adopted as the standard and preferred regimen for treating advanced pancreatic cancer. However, contribution of multidrug resistance protein 5 (MRP5) to gemcitabine cancer progression remains controversial. In present study, effect silencing MRP5 on cell proliferation migration human MIA Paca‑2 PANC‑1 cells was investigated by using short‑hairpin RNA delivered lentiviral vector transduction. The knockdown confirmed both mRNA levels qPCR surface...
Sperm-associated antigen 5 (SPAG5), also known as Astrin, was previously demonstrated a biomarker for cellular resistance to major breast cancer therapies, including chemo-, endocrine- and targeted therapy. However, the contribution of SPAG5 anthracycline- taxane-based chemotherapy in triple-negative (TNBC) remains controversial. In present study, knockout cell model established by using clustered regularly interspaced palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system...
Aidi injection is a traditional Chinese medicine containing multiple anti-tumour and immunomodulatory phytochemicals. While it synergistically enhances the efficacy of conventional chemotherapy in patients with non-small cell lung cancer (NSCLC), its effect on epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) sensitivity NSCLC remains unclear. This study aimed to investigate mechanisms human lines gefitinib. Effect gefitinib was assessed by MTT, colony formation...
Objective To construct a prokaryotic expression system for major histocompatibility complex class Ⅰ chain-related gene B (MICB) and to establish an ELISA method the detection of anti-MICB antibodies in patients with kidney transplantation. Methods The MICB cDNA fragments were obtained by RT-PCR pair specific primers. The vector pET-28a digested two restriction enzymes ligated recombinant plasmid pET-28a-MICB. transformed E. coli BL21 DE3 strains carrying induced IPTG express protein....
A total of 62 patients with radical operation esophageal carcinoma were divided into all-in-one nursing intervention group (research, n=32) and routine (control, n=30). With regards to postoperative flatus time, removal chest tube drainage hospital stay costs, the research was lower than control (P<0.05). After 2 weeks, from quality life questionnaire European Organization for Research Treatment Cancer, scores overall health enteral nutrition higher those parenteral while symptom fatigue,...
Objective To study the clinical effect of all-in-one nursing intervention to promote esophageal cancer patients' recovery.Methods 117 patients with squamous cell carcinoma who had radical resection from Jan Jun 2011 were enrolled in this study.63 cases surgery April June research group and received under fast recovery theory during perioperative period,while 54 January March control conventional nursing.Two groups' nutritional indexs before after surgery,recovery incidence complication was...
Abstract Background: A custom NGS cancer immune gene expression assay was developed which measures the transcript level of &gt;350 genes involved in T-cell receptor signaling (TCRS), tumor infiltrating lymphocyte (TILs) complement as well other key targets expected to predict likelihood patient response checkpoint inhibitors (CPI). In parallel assay, mutational profiling carried out using 409 Comprehensive Cancer Panel (ThermoFisher). As variability between runs is common when performing...
Objective To generate recombinant GPⅢa as an alternative source for HPA-1a antigen and combine it with Luminex xMAP beads the detection of HPA-1a-specific alloantibody. Methods The full coding region ITGB3 gene was amplified ligated pcDNA3.1. The plasmid transfected into CHO cells, those stable expression were screened G418. Expressed protein identified coupled beads, which then reacted sera samples. Subsequently, phycoerythrin-labeled anti-species IgG antibody added to reaction wells...
Abstract Background Esophageal cancer (EC) is the sixth deadliest in world. There has been no breakthrough research on EC past few decades. Epidermal growth factor-like protein 6 (EGFL6), as a member of epidermal factor superfamily, plays an important role occurrence and development some tumors. However, EGFL6 never explored. Methods Immunohistochemical staining was used to evaluate expression level EGEC6 human its adjacent non-tumor tissues, analyzed correlation between clinical...