A5090154416- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Diet, Metabolism, and Disease
- Eicosanoids and Hypertension Pharmacology
- Immune Response and Inflammation
- Liver Disease and Transplantation
- Anesthesia and Neurotoxicity Research
- Phagocytosis and Immune Regulation
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- Endoplasmic Reticulum Stress and Disease
- Acute Kidney Injury Research
- Neuropeptides and Animal Physiology
- Liver physiology and pathology
- NF-κB Signaling Pathways
- Cardiac Ischemia and Reperfusion
- Drug-Induced Hepatotoxicity and Protection
- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- Inflammasome and immune disorders
- COVID-19 and healthcare impacts
- Global Cancer Incidence and Screening
- Cytokine Signaling Pathways and Interactions
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Sirtuins and Resveratrol in Medicine
Wenzhou Medical University
2021-2023
Sinotech Genomics (China)
2023
National Institutes of Health
2014-2022
National Institute on Alcohol Abuse and Alcoholism
2014-2022
Peking University
2007-2017
Wuhan University
2017
University of Oklahoma Health Sciences Center
2012
Tokyo University of Science
2000
University of Arizona
1988
<h3>Objectives</h3> Chronic-plus-binge ethanol feeding activates neutrophils and exacerbates liver injury in mice. This study investigates how recent excessive drinking affects peripheral alcoholics, miR-223, one of the most abundant microRNAs (miRNAs) neutrophils, modulates neutrophil function ethanol-fed <h3>Designs</h3> Three hundred alcoholics with (n=140) or without (n=160) 45 healthy controls were enrolled. Mice fed an diet for 10 days followed by a single binge ethanol....
Obesity and alcohol consumption often coexist work synergistically to promote steatohepatitis; however, the underlying mechanisms remain obscure. Here, we demonstrate that feeding mice a high‐fat diet (HFD) for as little 3 days markedly exacerbated acute ethanol binge–induced liver neutrophil infiltration injury. Feeding with an HFD months plus single binge of induced much more severe steatohepatitis. Moreover, 3‐day or 3‐month HFD‐plus‐ethanol (3d‐HFD+ethanol 3m‐HFD+ethanol) treatment...
Leucine supplementation has been shown to prevent high-fat diet (HFD)-induced obesity, hyperglycemia, and dyslipidemia in animal models, but the underlying mechanisms are not fully understood. Recent studies suggest that activation of Sirtuin 1 (SIRT1) is an important mechanism maintain energy metabolic homeostasis. We therefore examined involvement SIRT1 leucine supplementation-prevented obesity insulin resistance. To accomplish this goal, male C57BL/6J mice were fed normal or HFD,...
Hepatic infiltration of neutrophils is a hallmark steatohepatitis; however, the role in progression steatohepatitis remains unknown.
Nonalcoholic fatty liver disease (NAFLD) represents a spectrum of diseases ranging from simple steatosis to more severe forms injury including nonalcoholic steatohepatitis (NASH), fibrosis, and hepatocellular carcinoma (HCC). In humans, only 20%-40% patients with progress NASH, mice fed high-fat diet (HFD) develop but are resistant NASH development. To understand how progresses we examined hepatic expression anti-inflammatory microRNA-223 (miR-223) found that this miRNA was highly elevated...
Over the last several years, one of major advances in field alcoholic liver disease research was discovery that binge alcohol consumption induced neutrophilia and hepatic neutrophil infiltration chronically ethanol-fed mice human subjects with excessive use (EAU); however, underlying mechanisms remain obscure. Here, we demonstrated chronic EAU patients a history recent drinking (EAU + RD) had higher serum levels mitochondrial DNA (mtDNA)-enriched microparticles (MPs) than without - healthy...
Alcoholic liver disease (ALD) is characterized by lipid accumulation and injury. However, how chronic alcohol consumption causes hepatic remains elusive. The present study demonstrates that activation of the mechanistic target rapamycin complex 1 (mTORC1) plays a causal role in alcoholic steatosis, inflammation, Chronic‐plus‐binge ethanol feeding led to hyperactivation mTORC1, as evidenced increased phosphorylation mTOR its downstream kinase S6 (S6K1) hepatocytes. Aberrant mTORC1 was likely...
Ciprofol is a newly developed intravenous sedative-hypnotic drug. The objective of the study was to prove whether ciprofol non-inferior propofol for successful induction general anesthesia. ideal post-induction sedation level assessed by comparing patients' clinical symptoms and their hemodynamic effects in responding noxious stimuli, mostly tracheal intubation bispectral index (BIS) alterations following ciprofol/propofol administration.In this multi-center, randomized, double-blind phase 3...
Alcohol use disorder (AUD) is a common and chronic with substantial effects on personal public health. The underlying pathophysiology poorly understood but strong evidence suggests significant roles of both genetic epigenetic components. Given that alcohol affects many organ systems, we performed cross-tissue cross-phenotypic analysis genome-wide methylomic variation in AUD using samples from 3 discovery, 4 replication, 2 translational cohorts. We identified differentially methylated region...
Obesity and alcohol consumption synergistically promote steatohepatitis, neutrophil infiltration is believed to be associated with steatosis. However, the underlying mechanisms remain obscure. Peroxisome proliferator–activated receptor gamma (PPARγ) plays a complex role in lipid metabolism inflammation; therefore, purpose of this study was dissect its regulating steatosis clinically relevant mouse steatohepatitis model 3‐month high‐fat diet (HFD) feeding plus binge ethanol (HFD‐plus‐binge...
Abstract Atherosclerosis is a chronic inflammatory disease with high morbidity and mortality rates worldwide. Doublecortin‐like kinase 1 (DCLK1), microtubule‐associated protein kinase, involved in neurogenesis human cancers. However, the role of DCLK1 atherosclerosis remains undefined. In this study, we identified upregulated macrophages atherosclerotic lesions ApoE −/− mice fed an HFD determined that macrophage‐specific deletion attenuates by reducing inflammation mice. Mechanistically, RNA...
Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), a key antibacterial protein, is highly elevated in patients with end-stage liver disease that often associated bacterial infection. LCN2 expressed at high levels both hepatocytes and neutrophils; however, how hepatocyte-derived neutrophil-derived cooperate to combat infection remains unclear. Here, by studying hepatocyte-specific myeloid-specific Lcn2 knockout mice two models of systemic local Klebsiella...
Objective Aldehyde dehydrogenase 2 (ALDH2), a key enzyme to detoxify acetaldehyde in the liver, exists both active and inactive forms humans. Individuals with ALDH2 accumulate after alcohol consumption. However, how affects T-cell hepatitis remains unknown. Design Wild-type (WT) Aldh2 knockout ( -/- ) mice were subjected chronic ethanol feeding concanavalin A (ConA)-induced hepatitis. Effects of on glucose metabolism investigated vitro. Human subjects recruited for binge drinking plasma...
Alcohol-associated liver disease is a spectrum of disorders with histopathological changes ranging from simple steatosis to steatohepatitis, cirrhosis, and hepatocellular carcinoma. Recent data suggest that chronic-plus-binge ethanol intake induces steatohepatitis by promoting release hepatocytes proinflammatory mitochondrial DNA–enriched (mtDNA-enriched) extracellular vesicles (EVs). The aim the present study was investigate role stress kinase apoptosis signal–regulating 1 (ASK1) p38...
Abstract Advanced fibrosis and portal hypertension influence short-term mortality. Lipocalin 2 (LCN2) regulates infection response increases in liver injury. We explored the role of intrahepatic LCN2 human alcoholic hepatitis (AH) with advanced experimental mouse fibrosis. found hepatic expression serum level markedly increased correlated disease severity patients AH. In control livers, expressed exclusively mononuclear cells, while its was induced AH not only cells but also notably...
Invariant natural killer T (iNKT) cells are a major subset of lymphocytes found in the liver. These mediate various functions, including hepatic injury, fibrogenesis, and carcinogenesis. However, function iNKT liver regeneration remains unclear. In present study, partial hepatectomy (PHx) was used to study regeneration. α-Galactosylceramide (α-GalCer), specific ligand for cells, induce cell activation. After PHx, two strains cell-deficient mice, CD1d(-/-) Jα281(-/-) showed normal Injection...
Cell ablation is a powerful tool for studying cell lineage and/or function; however, current cell-ablation models have limitations. Intermedilysin (ILY), cytolytic pore-forming toxin that secreted by Streptococcus intermedius, lyses human cells exclusively binding to the complement regulator CD59 (hCD59), but does not react with from nonprimates. Here, we took advantage of this feature ILY and developed model conditional targeted generating floxed STOP-CD59 knockin mice (ihCD59), in which...