Based on the antioxidant properties of pomegranate, this study was designed to investigate effects pomegranate peel extract damage associated with hypertension and aging in a spontaneously hypertensive rat (SHR) model. The influence consumption examined systolic blood pressure (SBP), angiotensin-converting enzyme (ACE) coronary activity, oxidative stress, vascular morphology. Four- or 28-wk-old SHR model rats were treated for 30 d, terminal experimental animal age being 8 32 wk,...

A decrease in female sex hormone levels the body impairs vascular endothelium functioning, leading to dysfunction associated with certain diseases. Animal models of ovariectomy are commonly used understand its effects on (dys)function. Fourier-transform infrared (FTIR) spectroscopy is a technique capable extracting detailed molecular information and, as such, has been applied various biological analyses. This study evaluated systemic changes model using mid-infrared spectroscopy....

The understanding of obsesity-related vascular dysfunction remains controversial mainly because the diseases associated with injury. Exercise training is known to prevent dysfunction. Using an obesity model without comorbidities, we aimed at investigating underlying mechanism and how exercise interferes this process.High-sugar diet was used induce in mice. performed 5 days/week. Body weight, energy intake, adipose tissues were assessed; blood metabolic hormonal parameters determined; serum...

The action of oestrogen has traditionally been attributed to the activation nuclear receptors (ERα and ERβ). A third receptor, G protein-coupled receptor (GPER), described as mediator rapid oestrogen. Based on possible protective role in cardiovascular system, present study was designed determine whether selective GPER induces relaxation mesenteric resistance arteries both sexes which signalling pathways are involved.Third-order were isolated, concentration-response curves plotted following...

The present study was designed to determine relaxation in response 17ß-estradiol by isolated perfused hearts from intact normotensive male and female rats as well the contribution of endothelium its relaxing factors this action. Baseline coronary perfusion pressure determined vasoactive effects (10 µM) were assessed bolus administration before after denudation infusion 0.25 µM sodium deoxycholate or with 100 L-NAME, 2.8 indomethacin, 0.75 clotrimazole, L-NAME plus clotrimazole. differed...

Abstract Aim: The relaxation induced by oestrogen in the coronary vascular bed from normotensive rats has been well described. However, almost nothing is known about this action spontaneously hypertensive (SHR). We investigated effect of 17 β‐oestradiol (E 2 ) arteries SHR as contribution endothelium and smooth muscle to action. Methods: Coronary male female were used. Mean arterial pressure (MAP) baseline perfusion (CPP) determined. effects 10 μ m E assessed bolus administration before...

Triorganotins, such as tributyltin (TBT), are environmental contaminants that commonly used antifouling agents for boats. However, TBT is also known to alter mammalian reproductive functions. Although the female sex hormones primarily involved in regulation of functions, 17β-estradiol protects against cardiovascular diseases, this hormone reduces incidence coronary artery disease via vasodilation. The aim study was examine influence 100 ng/kg administered daily by oral gavage 15 d on...

The aim of this study was to evaluate the effects swimming training (SW) and oestrogen replacement therapy (ERT) on coronary vascular reactivity expression antioxidant enzymes in ovariectomized rats. Animals were randomly assigned one five groups: sham (SH), (OVX), with E2 (OE2), exercise (OSW), plus (OE2+SW). SW protocol (5×/week, 60 min/day) and/or ERT conducted for 8 weeks; vasodilator response bradykinin analysed (Langendorff Method), (SOD-1 2, catalase) eNOS iNOS evaluated by Western...

Compared with age-matched men, premenopausal women are largely protected from coronary artery disease, a difference that is lost after menopause. The effects of oestrogens mediated by the activation nuclear receptors (ERα and ERβ) G protein-coupled oestrogen receptor (GPER). This study aims to evaluate potential role GPER in circulation female male rats. baseline perfusion pressure (CPP) concentration-response curve agonist (G-1) were evaluated isolated hearts before blockade GPER. GPER,...

Decline in estrogen levels promotes endothelial dysfunction and, consequently, the most prevalent cardiovascular diseases menopausal women. The use of natural therapies such as pomegranate can change these results. Pomegranate [Punica granatum L. (Punicaceae)] is widely used a phytotherapeutic agent worldwide, including Brazil. We hypothesized that treatment with hydroalcoholic extract (PHE) would improve coronary vascular reactivity and parameters. At beginning treatment, spontaneously...

Drospirenone (DRSP) is a progestin with anti-aldosterone properties and it reduces blood pressure in hypertensive women. However, the effects of DRSP on endothelium-dependent coronary vasodilation have not been evaluated. This study investigated combined therapy estrogen (E2) bed ovariectomized (OVX) spontaneously rats. Female rats (n=87) at 12 weeks age were randomly divided into sham operated (Sham), OVX, OVX treated E2 (E2), (E2+DRSP) groups. Hemodynamic parameters directly evaluated by...

Estrogen deficiency and hypertension are considered major risk factors for the development of coronary heart disease. On other hand, exercise training is an effective form to prevent treat cardiovascular diseases. However, effects swimming (SW) on vascular reactivity in female ovariectomized hypertensive rats not known. We aimed evaluate SW endothelium-dependent vasodilation rats. Three-month old spontaneously (SHR, n=50) were divided into four groups: sham (SH), plus (SSW), (OVX), (OSW)....

Abstract Diminazene aceturate (DIZE) has been described as an angiotensin‐converting enzyme 2 (ACE2) activator. We aimed to investigate DIZE effects on blood pressure (BP) of spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats. BP was recorded in awake unrestrained 24 hours after femoral artery catheterization. (15 mg/kg, s.c.) produced a fast decrease only SHR ( P < .01). Pre‐treatment with L‐NAME (10 iv) did not change the hypotensive effect systolic whereas mitigated...

The present study aimed to determine the effects of chronic treatment with different doses testosterone on endothelium-dependent coronary vascular reactivity in male rats. Adult rats were divided into four experimental groups: control (SHAM), castrated (CAST), and immediately treated subcutaneously a physiological dose (0.5 mg/kg/day, PHYSIO group) or supraphysiological (2.5 SUPRA for 15 days. Systolic blood pressure (SBP) was assessed at end through tail plethysmography. After euthanasia,...

The relaxation of coronary arteries by estrogens in the vascular beds naive and hypertensive rats has been well described. However, little is known about this action gonadectomized rats. We investigated effect 17-ß-estradiol (E2) from rats, as contributions endothelium-derived factors potassium channels. Eight-week-old female male Wistar weighing 220-300 g were divided into sham-operated groups (n=9-12 animals per group). baseline perfusion pressure (CPP) was determined, vasoactive effects...

Several studies have demonstrated that gonadal hormones show significant effects on the brain and signaling pathways of effector organs/cells respond to neurotransmitters. Since little information is available concerning impact male female renal peripheral sympathetic system, objective this study was further assess whether how content plasma concentration catecholamines are influenced by gender estrous cycle in rats. To achieve this, males Wistar rats were divided into 4 groups: (i) sham...

Background The protective effect of estrogen on the vasculature cannot be explained only by its action through receptors ERα and ERβ. G protein-coupled (GPER)—which are widely distributed throughout cardiovascular system—may also involved in this response. However, little is known about GPER actions hypertension. Therefore, study we evaluated vascular response mediated using a specific agonist, G-1, spontaneously hypertensive rats (SHR). We hypothesized that G-1 would induce relaxing...

Ultrasound lipoclasia (USL) on white adipose tissue (WAT) has been largely used in the treatment of cellulite. Nevertheless, acute consequences this therapy metabolism and biochemical profile are significant should be taken into account.To analyze metabolic effects USL WAT healthy rats using analyses body composition, profile, inflammatory markers.Female Wistar weighing approximately 250 g were divided two groups (n=10 each): control treated. The treated group was submitted to USL, a single...

Although progesterone (P4) has a beneficial effect on the cardiovascular system, P4 actions coronary bed have not yet been fully elucidated. This study evaluated of treatment endothelium-dependent vascular reactivity in Wistar rats.Eight-week-old adult rats were divided into Sham, Ovariectomized (OVX), and treated (OVX P4). The OVX group received daily doses (2 mg/kg/day). Vascular was assessed by modified Langendorff technique. intensity eNOS, Akt, gp91phox protein expression quantified...

Physiological or supraphysiological levels of testosterone appear to be associated with the development risk factors for cardiovascular diseases such as hypertension, this hormone modulates release endothelial factors. However, its actions are still controversial, especially in coronary circulation hypertensive animals. This study was designed assess effects treatment (T) on endothelium-dependent vascular reactivity orchiectomized SHR. The animals were divided into SHAM, (ORX), ORX+T and...

Progesterone seems to play a role in cardiovascular physiology since its receptors are expressed on endothelial cells from both sexes of mammals. However, little is known about the coronary circulation. Thus, this study aims evaluate effect acute administration progesterone bed and pathways involved action normotensive rats sexes. A dose–response curve (1–50 μmol/L) isolated hearts using Langendorff preparation was performed. Baseline perfusion pressure (CPP) determined, vasoactive evaluated...