A5045332296- Spondyloarthritis Studies and Treatments
- Dermatology and Skin Diseases
- Psoriasis: Treatment and Pathogenesis
- Antimicrobial Peptides and Activities
- Gut microbiota and health
- Systemic Lupus Erythematosus Research
- Immune Response and Inflammation
- Autoimmune and Inflammatory Disorders Research
- Inflammatory Bowel Disease
- Renal Transplantation Outcomes and Treatments
- Microscopic Colitis
- Pharmacological Effects of Natural Compounds
- Reproductive System and Pregnancy
- Reproductive tract infections research
- Tryptophan and brain disorders
- Pharmacological Effects and Toxicity Studies
- Pediatric health and respiratory diseases
- Immunotherapy and Immune Responses
- Inflammasome and immune disorders
- Macrophage Migration Inhibitory Factor
- Oral Health Pathology and Treatment
- Clostridium difficile and Clostridium perfringens research
- Immune Cell Function and Interaction
- Streptococcal Infections and Treatments
- Electrolyte and hormonal disorders
Aurinia (Canada)
2021-2025
The University of Queensland
2012-2024
Translational Research Institute
2015-2022
Princess Alexandra Hospital
2014-2022
University of British Columbia
2006-2010
The spondylarthritides (SpA), including ankylosing spondylitis (AS), psoriatic arthritis (PsA), reactive arthritis, and associated with inflammatory bowel disease, cause chronic inflammation of the large peripheral axial joints, eyes, skin, ileum, colon. Genetic studies reveal common candidate genes for AS, PsA, Crohn's IL23R, IL12B, STAT3, CARD9, all which are interleukin-23 (IL-23) signaling downstream dectin 1 β-glucan receptor. In autoimmune-prone SKG mice mutated ZAP-70, attenuates T...
Objective Spondyloarthritides (SpA) occur in 1% of the population and include ankylosing spondylitis (AS) arthropathy inflammatory bowel disease (IBD), with characteristic spondylitis, arthritis, enthesitis, IBD. Genetic studies implicate interleukin‐23 (IL‐23) receptor signaling development SpA IBD, IL‐23 overexpression mice is sufficient for driven by entheseal‐resident T cells. However, genetically prone individuals, it not clear where produced how drives syndrome, including IBD or...
Abstract The human cathelicidin LL-37 is a cationic host defense peptide and serves as an important component of innate immunity. It has been demonstrated to be multifunctional modulator immune responses, although the mechanism(s) underlying this have not well characterized. In study, it was that synergistically enhanced IL-1β-induced production cytokines (IL-6, IL-10) chemokines such macrophage chemoattractant proteins (MCP-1, MCP-3) in PBMC, indicating role enhancing certain responses....
The spondyloarthritides share genetic susceptibility, interleukin-23 (IL-23) dependence, and the involvement of microbiota. aim current study was to elucidate how host genetics influence gut microbiota relationship between organ inflammation in spondyloarthritides.BALB/c ZAP-70(W163C) -mutant (SKG) mice, Toll-like receptor 4 (TLR-4)-deficient SKG wild-type BALB/c mice were housed under specific pathogen-free conditions. maintained germ-free conditions, some these recolonized with altered...
In our previous study (Chen et al. J Biol Chem 2005, 280:12316–12329), we utilized an α ‐helical antimicrobial peptide V 681 as the framework to effects of hydrophobicity, amphipathicity, and helicity on biologic activities where obtained several analogs with dramatic improvement in therapeutic indices against gram‐negative gram‐positive bacteria. present study, d ‐enantiomers three peptides – , V13A D V13K L were synthesized compare biophysical properties their enantiomeric isomers. Each...
Objectives Certain gut bacterial families, including Bacteroidaceae, Porphyromonadaceae and Prevotellaceae, are increased in people suffering from spondyloarthropathy (SpA), a disease group associated with IL23R signalling variants. To understand the relationship between host interleukin (IL)-23 dysbiosis SpA, we inhibited IL-23 dysbiotic ZAP-70-mutant SKG mice that develop IL-23-dependent SpA-like arthritis, psoriasis-like skin inflammation Crohn’s-like ileitis response to microbial beta...
Analysis of oral dysbiosis in individuals sharing genetic and environmental risk factors with rheumatoid arthritis (RA) patients may illuminate how microbiota contribute to disease susceptibility. We studied the a prospective cohort RA, first-degree relatives (FDR) healthy controls (HC), then genomically functionally characterised streptococcal species from each group understand their potential contribution RA development.After DNA extraction tongue swabs, targeted 16S rRNA gene sequencing...
Kidney injury in patients with lupus nephritis (LN) results pro-fibrotic biomarker expression, a manifestation also observed calcineurin inhibitor (CNI) therapy. The second-generation CNI, voclosporin, is approved the United States and Europe for treatment of active LN combination background immunosuppression, based on successful outcomes from global phase 2 AURA-LV 3 AURORA 1 studies, which demonstrated efficacy voclosporin across diverse racial ethnic populations, encompassing multiple...
LL-37, the only member of cathelicidin family cationic host defence peptides in humans, has been shown to mediate multiple immunomodulatory effects and as such is thought be an important component innate immune responses. A growing body evidence indicates that LL-37 affects lung mucosal responses pathogens through altered regulation cell migration, proliferation, wound healing apoptosis. These functions are consistent with playing a role regulating epithelial inflammatory responses; however,...
Chlamydia trachomatis is a sexually transmitted obligate intracellular pathogen that causes inflammatory reactive arthritis, spondylitis, psoriasiform dermatitis, and conjunctivitis in some individuals after genital infection. The immunologic basis for this response susceptible hosts poorly understood. As ZAP-70(W163C) -mutant BALB/c (SKG) mice are to spondylo-arthritis systemic exposure microbial β-glucan, we undertook the present study compare responses infection with muridarum SKG...
Host defense peptides are a critical component of the innate immune system. Human alpha- and beta-defensin genes subject to copy number variation (CNV) historically organization mouse alpha-defensin has been poorly defined. Here we present first full manual genomic annotation defensin region on Chromosome 8 reference strain C57BL/6J, analysis orthologous regions human rat genomes. Problems were identified with assemblies all three Defensins have studied for over two decades their naming...
Calcineurin inhibitors (CNIs) affect kidney electrolyte handling and blood pressure (BP) through an effect on the distal tubule. The second-generation CNI voclosporin causes hypomagnesaemia hypercalciuria less often than tacrolimus. This suggests different effects tubule, but this has not yet been investigated experimentally.
ZAP-70W163C BALB/c (SKG) mice develop reactive arthritis (ReA) following infection with Chlamydia muridarum. Since intracellular pathogens enhance their replicative fitness in stressed host cells, we examined how myeloid cells infected C muridarum drive arthritis.SKG, Il17a-deficient SKG, and female were or luciferase the genitals. dissemination was assessed by vivo imaging genomic DNA amplification. Macrophages depleted using clodronate liposomes. Anti-tumor necrosis factor (anti-TNF)...
During the course of its infection mammalian digestive tract, entero-invasive, Gram-negative bacterium Yersinia pseudotuberculosis must overcome various hostile living conditions (notably, iron starvation and presence antimicrobial compounds produced in situ). We have previously reported that vitro bacterial growth during deprivation raises resistance to peptide polymyxin B; here, we show this phenotype is mediated by a chromosomal gene (YPTB0333) encoding transcriptional regulator from LysR...
<h3>Background:</h3> The gut-joint axis exists in spondyloarthropathy, such as ankylosing spondylitis and psoriatic arthritis, where arthritis is often associated with gut dysbiosis inflammation. Type 3 immune cytokines IL-17, IL-22 IL-23 are implicated SpA pathogenesis. Pharmaceutical agents neutralising these clinical use but have variable response rates different conditions. After systemic β-1,3-glucan (curdlan) injection, ZAP70<sup>W163C</sup> SKG mice develop IL-23-dependent SpA-like...
Lupus nephritis (LN) is a major cause of morbidity and mortality with lifetime incidence up to 60% in systemic lupus erythematosus (SLE).1 Cardiovascular diseases (CVD) are also the leading death patients SLE.2 LN an independent risk factor for CVD which increases by two times as compared SLE without LN.3 Goals treatment include preserving kidney function reducing mortality, while minimizing related adverse events improving quality life.