A5076614943- Influenza Virus Research Studies
- Respiratory viral infections research
- COVID-19 epidemiological studies
- Animal Disease Management and Epidemiology
- COVID-19 Clinical Research Studies
- Viral Infections and Immunology Research
- HIV Research and Treatment
- HIV-related health complications and treatments
- SARS-CoV-2 and COVID-19 Research
- Neonatal Health and Biochemistry
- COVID-19 Pandemic Impacts
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- Regional Development and Management Studies
- Adipokines, Inflammation, and Metabolic Diseases
- CRISPR and Genetic Engineering
- Sustainability and Innovation in Business
- Malaria Research and Control
- Dermatological and COVID-19 studies
- Chemokine receptors and signaling
- Respiratory Support and Mechanisms
- COVID-19 Impact on Reproduction
- RNA Interference and Gene Delivery
- Trypanosoma species research and implications
- Hematopoietic Stem Cell Transplantation
Pasteur Hellenic Institute
2013-2022
University of California, Los Angeles
2017-2022
Office of Infectious Diseases
2020
National Laboratory of Health, Environment and Food
2017
Instituto de Salud Carlos III
2017
Institut Pasteur
2013
Eginition Hospital
2013
Abstract Background There is limited information on the association between upper respiratory tract (URT) viral loads, host factors, and disease severity in SARS-CoV-2–infected patients. Methods We studied 1122 patients (mean age, 46 years) diagnosed by polymerase chain reaction (PCR). URT load, measured PCR cycle threshold, was categorized as high, moderate, or low. Results were 336 (29.9%) with comorbidities; 309 (27.5%) had 316 (28.2%) 497 (44.3%) low load. In univariate analyses,...
There is limited information on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection clustering within families with children. We aimed to study the transmission dynamics of SARS-CoV-2 children in Greece. studied 23 family clusters disease 2019 (COVID-19). Infection was diagnosed by reverse-transcriptase polymerase chain reaction specimens. The level viral load categorized as high, moderate, or low based cycle threshold values. were 109 household members (66 adults and 43...
On 18 April 2014, a case of Middle East Respiratory Syndrome coronavirus (MERS-CoV) infection was laboratory confirmed in Athens, Greece patient returning from Jeddah, Saudi Arabia. Main symptoms upon initial presentation were protracted fever and diarrhoea, during hospitalisation he developed bilateral pneumonia his condition worsened. During 14 days prior to onset illness, had extensive contact with the healthcare environment Jeddah. Contact tracing revealed 73 contacts, no secondary cases...
The epidemiology of seasonal influenza is influenced by age. During the season, European Influenza Surveillance Network (EISN) reports weekly virological and syndromic surveillance data [mostly influenza-like illness (ILI)] based on national networks sentinel primary-care providers. Aggregated numbers age group are available for ILI, but not linked to data. At end season 2012/2013, all EISN laboratories were invited submit a subset their this including information analysis suggests that...
Novel therapeutic strategies are needed to attenuate increased systemic and gut inflammation that contribute morbidity mortality in chronic HIV infection despite potent antiretroviral therapy (ART). The goal of this study is use preclinical models treated determine whether the antioxidant anti-inflammatory apoA-I mimetic peptides 6F 4F HIV. We used two humanized murine explants from 10 uninfected infected persons on ART, vivo ex impact mimetics intestinal When compared mice with ART alone,...
The current study aimed to describe the molecular epidemiology of mixed respiratory viral infections during consecutive winter seasons in a tertiary care hospital. Patients with symptoms tract infection were evaluated 2009‐2011 and 2013‐15 seasons. A clinical microarray technique was used for detection. Clinical epidemiological data correlated detection need hospitalization. In 332 out 604 (54.4%) patients (17.6% children) virus identified. Mixed diagnosed 68/332 (20.5%) (66.2% Influenza‐RSV...
Transfection is one of the most frequently used techniques in molecular biology that also applicable for gene therapy studies humans. One biggest challenges to investigate protein function and interaction have reliable monospecific detection reagents, particularly antibodies, all human products. Thus, a method can optimize transfection efficiency based on not only expression target interest but uptake nucleic acid plasmid, be an important tool biology. Here, we present simple, rapid robust...
Viral infections are a significant cause of morbidity and mortality in pediatric transplant populations. We analyzed the epidemiology viral hematopoietic stem cell (HSCT) patients, including their incidence, associated risk factors, outcome.In prospective study from September 2011 to 2015, blood, urine, stool specimens were monitored weekly transplantation day 100 or after if clinically suspected, by use real-time polymerase chain reaction. Cytomegalovirus (CMV), Epstein-Barr virus (EBV), BK...
Th17 cytokines are associated with modulation of inflammation and may be beneficial in clearing influenza infection experimental models. The cytokine profile was evaluated a pilot study respiratory virus infections.Consecutive patients symptoms tract visiting the emergency department tertiary care hospital during winter season 2014 to 2015 were evaluated. CLART PneumoVir kit, (GENOMICA, Madrid, Spain) used for viral detection all known viruses. MILLIPLEX MAP Human TH17 Magnetic Bead Panel...
The genotypic analysis of human metapneumo-(HMPV) and boca-(HBoV) viruses circulating in Greece their comparison to reference other clinical strains.Genetic representative strains over three consecutive winter seasons the years 2005-2008.Representative positive specimens for HMPV HBoV from paediatric patients healthcare units hospitals Southern with influenza-like illness or respiratory tract infections.Seven ten either each period. In total, 24 26 HBoV, respectively.Sequence diversity by...
Rapid and reliable laboratory diagnosis of persons suspected Middle East respiratory syndrome coronavirus (MERS-CoV) infection is important for timely implementation control practices disease management. In addition, monitoring molecular changes in the virus can help elucidate chains transmission identify mutations that might influence efficiency. This was illustrated by a recent investigation we conducted on an imported MERS-CoV case Greece. Two oropharyngeal swab specimens were collected...
Objective(s): Despite antiretroviral therapy (ART), there is an unmet need for therapies to mitigate immune activation in HIV infection. The goal of this study determine whether the apoA-I mimetics 6F and 4F attenuate macrophage chronic HIV. Design: Preclinical assessment in-vivo impact Tg6F ex-vivo on biomarkers gut barrier dysfunction treated Methods: We used two humanized murine models infection oral with ART (HIV + ) innate (plasma human sCD14, sCD163) [murine I-FABP, endotoxin (LPS),...
The 2014–2015 influenza season was marked by circulation of antigenically drifted A/H3N2 strains, raising the possibility low seasonal Vaccine Effectiveness (VE). We assessed VE against hospitalization with laboratory-confirmed for season, using routine surveillance data. Non-sentinel swab samples from Greek hospital inpatients were tested RT-PCR in three laboratories, covering entire country. estimated a test-negative design. Out 883 patients known vaccination status, 161 (18.2%)...
ABSTRACT Genetic and antigenic characterization of 37 representative influenza A(H3N2) virus strains isolated in Greece during the 2011-2012 winter season was performed to evaluate matching viruses with seasonal vaccine strain A/Perth/16/2009. Hemagglutinin gene sequence analysis revealed that all Greek clustered within Victoria/208 genetic clade. Furthermore, substitutions glycosylation sites suggested potential drift. Our hemagglutination inhibition (HI) showed were Perth/16-like; however,...
The 2018/2019 influenza season in the WHO European Region was dominated by A (H1N1)pdm09 and (H3N2) viruses, with very few B viruses detected. Countries reported virus characterization data to Surveillance System for weeks 40/2018 20/2019. These antigenic genetic haemagglutinin (HA) sequence were analysed describe assess circulating relative vaccine components northern hemisphere. Thirty countries 4776 characterized genetically 3311 antigenically. All A(H1N1)pdm09 fell subclade 6B.1A, of...
Influenza antigenic and genetic characterisation data are crucial for influenza vaccine composition decision making. Previously, aggregate were reported to the European Centre Disease Prevention Control by Union/European Economic Area (EU/EEA) countries. A system collecting case-specific was established 2013/14 season. In a pilot study, 11 EU/EEA countries through new mechanism. We demonstrated feasibility of reporting strain-based ca 10% virus-positive specimens selected further...
Influenza is associated with an increased risk for serious illness, hospitalization, and death in pregnant women young infants. Our aim was to estimate the effectiveness of a quadrivalent inactivated influenza vaccine (QIV) their infants during 2019-2020.A QIV offered followed maternity hospital. Women were contacted weekly season asked about symptoms. Polymerase chain reaction testing pharyngeal samples influenza-like illness. A Bayesian beta-binomial model used.We studied 636 (406...
Abstract Malaria has become an emerging infection in Greece, which is the doorstep to Europe for thousands of immigrants. With increasing immigration, cases with evidence domestic transmission (autochthonous) are being reported. In present study, isolate Plasmodium vivax from autochthonous clinical case was subjected phylogenetic analysis genes encoding merozoite surface protein 1 (MSP-1) and circumsporozoite (CSP). MSP region, strain related strains Brazil, South Korea, Turkey Thailand,...
We investigated whether apolipoprotein A-I (apoA-I) mimetic peptides 4F and 6F can be a novel therapeutic strategy to reduce blood gut bioactive lipids, proinflammatory effects of endotoxin (LPS) aberrant activation cyclooxygenase 2 (COX-2) as instigators increased risk for cardiometabolic disease in chronic treated HIV.We used two humanized murine models HIV infection (n = 109 mice) explants from infected 10) persons determine Tg6F attenuate vivo ex lipids (measured by mass spectrometry)...
Congenital human cytomegalovirus infection (cCMV) is the commonest congenital infection, and it can result in hearing loss neurodevelopmental delay. Even if primary infections are more frequent cause severe cCMV manifestations compared to NPIs, despite partial protection from maternal immunity, highest birth prevalence of observed seropositive women with non-primary CMV (NPI). Given that NPI contribute significantly overall burden cCMV, their accurate diagnosis remains clinically important....