A5090596838- Vector-borne infectious diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- Orthopedic Infections and Treatments
- Diphtheria, Corynebacterium, and Tetanus
- COVID-19 and healthcare impacts
- Antimicrobial Resistance in Staphylococcus
- Intramuscular injections and effects
- Vector-Borne Animal Diseases
- Infectious Diseases and Tuberculosis
- COVID-19 and Mental Health
- Antifungal resistance and susceptibility
- Pneumocystis jirovecii pneumonia detection and treatment
- Long-Term Effects of COVID-19
- Bacterial Identification and Susceptibility Testing
- Healthcare professionals’ stress and burnout
- Infective Endocarditis Diagnosis and Management
- Bacillus and Francisella bacterial research
- SARS-CoV-2 and COVID-19 Research
- Orthopedic Surgery and Rehabilitation
- Amoebic Infections and Treatments
- HIV/AIDS drug development and treatment
- Fungal Infections and Studies
- Medical and Biological Ozone Research
Nemocnice České Budějovice
2016-2025
University of South Bohemia in České Budějovice
2019-2024
University of Liverpool
2012-2023
Royal Liverpool University Hospital
2012-2023
Public Health England
2019
Hradecký Písek (Czechia)
2019
Health Protection Research Unit in Emerging and Zoonotic Infections at University of Liverpool
2019
National Institute for Health Research
2019
Liverpool School of Tropical Medicine
2019
Medical Research Council
2017
BackgroundStaphylococcus aureus bacteraemia is a common cause of severe community-acquired and hospital-acquired infection worldwide. We tested the hypothesis that adjunctive rifampicin would reduce bacteriologically confirmed treatment failure or disease recurrence, death, by enhancing early S killing, sterilising infected foci blood faster, reducing risks dissemination metastatic infection.MethodsIn this multicentre, randomised, double-blind, placebo-controlled trial, adults (≥18 years)...
Aspergillus bronchitis is poorly understood and described. We extracted clinical data from more than 400 referred patients with persistent chest symptoms who did not fulfill criteria for allergic, chronic, or invasive aspergillosis. Symptomatic a positive culture real-time PCR spp. were reviewed. Seventeen fulfilled the selected criteria. Fourteen women, mean age of 57 years (range 39-76). Sixteen had productive cough, eight voluminous tenacious sputum, seven recurrent infections. Eight...
Tick-borne encephalitis virus (TBEV) is an emerging human pathogen that causes potentially fatal disease with no specific treatment. Mouse monoclonal antibodies are protective against TBEV, but little known about the antibody response to infection. Here, we report on neutralizing TBEV in a cohort of infected and vaccinated individuals. Expanded clones memory B cells expressed closely related anti-envelope domain III (EDIII) both groups volunteers. However, most potent antibodies, IC50s below...
Tick-borne infections are the most common vector-borne diseases in USA. Ticks harbor and transmit several with Lyme disease being tickborne infection US Europe. Lack of awareness about tick populations, specific diagnostic tests, overlapping signs symptoms tick-borne can often lead to misdiagnosis affecting treatment prevalence data reported especially for non-Lyme infections. The tests currently available severely limited their ability provide accurate results cannot detect multiple...
Vaccination against tick-borne encephalitis (TBE) is based on the use of formalin-inactivated, culture-derived whole-virus vaccines. Immune response following vaccination primarily directed to viral envelope (E) protein, major surface antigen. In Europe, two TBE vaccines are available in adult and pediatric formulations, namely FSME-IMMUN® (Pfizer) Encepur® (GlaxoSmithKline). Herein, we analyzed content these using mass spectrometry (MS). The MS analysis revealed that Encepur vaccine...
A brain abscess caused by Encephalitozoon cuniculi genotype I together with Streptococcus intermedius occurred in a patient without major immunocompromise and diabetes. The distinguishing clinical signs were hemiparesis epilepsy. microsporidium was observed the aspirate, its specific DNA also detected stool urine. successfully treated albendazole mebendazole.
Matrix metalloproteinases (MMPs) play an important role in central nervous system infections. We analysed the levels of 8 different MMPs cerebrospinal fluid (CSF) 89 adult patients infected with tick-borne encephalitis (TBE) virus and compared them a control group. MMP-9 was only MMP that showed significantly increased CSF TBE patients. Serum were subsequently measured 101 at various time points during neurological phase follow-up. In addition, serum 37 paediatric Compared levels, both had...
Over 480 000 Ukrainian refugees have arrived in the Czech Republic since Russian invasion of Ukraine 2022, including over 500 people with HIV. This study describes demographics, characteristics, and management HIV Republic.Retrospective, observational, noninterventional study.Ukrainian nationals registering at centers war refugee status were included. Data collected from medical records between 1 March 31 July 2022. The was registered State Institute for Drug Control, ID number...
Alternative modes of antiretroviral administration are sought for people with impaired intestinal passage and/or absorption. We present a case late HIV diagnosis (CD4+ count 160 cells/µL) gastric outlet obstruction due to stomach adenocarcinoma. Co-morbidities included oesophageal candidiasis, Helicobacter pylori-positive duodenal ulcers and cytomegalovirus duodenitis. The required total parenteral nutrition medication during four weeks diagnostic work-up leading pyloric resection. Crushed...
Several cases of capillary leak syndrome (CLS) related to COVID-19 or vaccination against SARS-CoV-2 have been described in the literature. We present a case 42-year-old, previously healthy male, presenting with mild form COVID-19, who suddenly developed severe shock hypotension and hemoconcentration within hours admission hospital. Volume resuscitation was not effective, increasing hemoglobin (198 g/L on admission, 222 9 h later) suggested fluid into peripheral tissues. After cardiac...
Previous studies of raltegravir and rifampicin have not studied the interaction when is dosed intermittently. This study aimed to assess pharmacokinetics twice daily intermittently rifampicin. was a prospective, open, single-arm, three-part, controlled in healthy volunteers. Over period 38 days subjects received 5 standard-dose (400 mg daily) followed by 28 plus three times week high-dose (800 week. Pharmacokinetic sampling performed on 5, 33 38. Raltegravir pharmacokinetic parameters were...
We retrospectively assessed the utility of a flow cytometry-based test quantifying percentage CD3+ T cells with CD4-/CD8- phenotype for predicting tularemia diagnoses in 64 probable and confirmed patients treated during 2003-2015 342 controls tularemia-like illnesses 2012-2015 Czech Republic. The median CD3+/CD4-/CD8- peripheral blood was higher (19%, 95% CI 17%-22%) than (3%, 2%-3%). When we used 8% as cutoff, this test's sensitivity 0.953 specificity 0.895 distinguishing cases from...
PURPOSE OF THE STUDY This study aims to articulate regional guidelines for curative and suppressive antibiotic therapy of total joint replacement infections. MATERIAL AND METHODS When developing the standard, used as source materials were published foreign prosthetic infections, analysis resistance bacterial strains conducted in Hospital České Budějovice, a.s. assessment strain Czech Republic by European Antimicrobial Resistance Surveillance Network (EARS-Net). Considered was also...
Extensive axonal and neuronal loss is the main cause of severe manifestations poor outcomes in tick-borne encephalitis (TBE). Phosphorylated neurofilament heavy subunit (pNF-H) an essential component axons, its detection cerebrospinal fluid (CSF) or serum can indicate degree neuroaxonal damage. We examined use pNF-H as a biomarker injury TBE. In 89 patients with acute TBE, we measured CSF levels 3 other markers brain (glial fibrillary acidic protein, S100B ubiquitin C-terminal hydrolase L1)...
Aim: To assess the expectations of Czech doctors during COVID-19 pandemic relative to ability nurses use telemedicine technologies. Design: descriptive and cross-sectional Participants: 1,203 physicians were included in study. The sample is representative terms gender, age, region. Methods: A sociological survey was conducted. non-standardized questionnaire used collect data. field conducted using standardized face-toface interviews. Results: Most agree that can learn how technologies these...
A 33-year-old previously healthy male presented with a 1-week history of severe sore throat and fever. He had been diagnosed acute tonsillitis by his primary care physician and, although not allergic to beta-lactams, treated empirically clarithromycin. On admission he was febrile dyspneic, bilateral exudative symmetrical anterior cervical lymphadenopathy. Computed tomography revealed right facial vein thrombosis soft tissue stranding (Figure 1A ), internal jugular 1B), right-sided pleural...
Východisko:Terapeutické monitorování hladin vankomycinu má zásadní význam pro úspěšnost a bezpečnost léčby. Doporučený postup dávkování podle Scottish Antimicrobial Prescribing Group určuje velikost úvodní dávky tělesné hmotnosti, další 3-4 clearance kreatininu následné se upravuje naměřených údolních hladin. Metody:Retrospektivní kohortová studie všech dospělých pacientů léčených vankomycinem před po převzetí doporučeného postupu v roce 2015 porovnává rychlost dosažení doporučených (10-20...