A5101651468- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Viral Infections and Vectors
- Fire effects on ecosystems
- Mosquito-borne diseases and control
- Infectious Diseases and Tuberculosis
- COVID-19 Clinical Research Studies
- Drug-Induced Hepatotoxicity and Protection
- SARS-CoV-2 and COVID-19 Research
- Amoebic Infections and Treatments
- Tuberculosis Research and Epidemiology
- Streptococcal Infections and Treatments
- Urinary Tract Infections Management
- Cancer-related molecular mechanisms research
- Antimicrobial Peptides and Activities
- Inflammatory Bowel Disease
- Liver Diseases and Immunity
- PI3K/AKT/mTOR signaling in cancer
- Bartonella species infections research
- Pediatric health and respiratory diseases
- Machine Learning in Bioinformatics
- Immune Response and Inflammation
- Pancreatitis Pathology and Treatment
- Advanced Proteomics Techniques and Applications
Hangzhou Medical College
2017-2022
Zhejiang Provincial People's Hospital
2015-2022
Fudan University
2020
Zhoushan Hospital
2010-2020
Zhejiang Hospital
2016-2019
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2008
Zhejiang University
2008
Hepatocellular carcinoma (HCC) is a prevalent malignant tumor with high morbidity and mortality across the world. Recent findings have suggested that long noncoding (lnc)RNA HOXA-AS3 plays an important role in tumorigenesis metastasis variety of cancers. However, lncRNA initiation progression HCC remains largely unclear. In present study, was highly expressed tissues cell lines. High expression correlated low survival patients. Loss-of-function experiments showed knockdown inhibited...
ObjectivesHere, we retrospectively described the diagnosis and treatment of 32 cases diagnosed with Chlamydia psittaci pneumonia during COVID-19 pandemic.MethodsClinical information was collected from all patients. Reverse transcription–PCR ELISAs were conducted for detection using nasal swabs bronchoalveolar lavage fluid (BALF) samples. Metagenomic next-generation sequencing (mNGS) performed identification causative pathogens BALF, peripheral blood sputum End-point PCR to confirm mNGS...
Abstract Chronic HBV (CHB) infected patients with intermediate necroinflammation and fibrosis are recommended to receive antiviral treatment. However, other than liver biopsy, there is a lack of sensitive specific objective method determine the stages in CHB patients. This study aims identify unique serum metabolomic profile associated histological progression develop novel metabolite biomarker panels for early detection stratification. A comprehensive profiling was established compare...
Phosphatidylcholine (PC) has been demonstrated as anti-inflammatory and antioxidant/pro-oxidant molecules. In this study, we investigated the protective effects of PC on inflammatory bowel disease (IBD) caused by lipopolysaccharide (LPS)-induced injury in intestinal epithelia cells. The IEC-6 cells (intestinal cells) were stimulated with LPS (1 μg/mL) for 24 h or without pretreatment, next steps: (1) level cytokine tumor necrosis factor (TNF)-α was measured ELISA; (2) nuclear translocation...
Rationale: Peliosis hepatis (PH) is a rare tumor-like liver lesion composed of multiple blood-filled cavities within the parenchyma. It hard to differentiate PH from other lesions by imaging, such as carcinoma, metastases, or abscess. Patient concerns: Here, we reported 2 cases that presented with under ultrasound and computed tomography (CT) scanning, without any history diseases drug usage traced back. Diagnoses: Liver biopsy laparoscopy were processed, eventually diagnosed histopathology,...
The high rate of relapse after cessation nucleos(t)ide analogues (NUCs) treatment in chronic hepatitis B (CHB) patients leads us to reassess the feasibility for off-therapy, but long-term follow-up data are scarce. We assessed off-therapy by a observation response lamivudine (LAM) and telbivudine (LdT). Eighty-six NUC-naive CHB patients, treated with LAM (n = 46) or LdT 40) who reached guidelines recommended were followed up 10 years. Hepatitis virus (HBV), viral serology biochemistries...
Combination therapy is an important method for treating advanced hepatocellular carcinoma (HCC). Gefitinib epidermal growth factor receptor (EGFR) inhibitor, which has profound effects on HCC. The purpose of the present study was to investigate genistein in combination with gefitinib proliferation and apoptosis HCC cells associated mechanism. Cell counting kit‑8 assay performed calculate IC50 values cytotoxicity, whilst flow cytometry used assess cell apoptosis. Protein expression detected...
Tuberculous meningitis (TBM) is the most common form of central nervous system tuberculosis with a very poor prognosis. We aimed at assessing risk factors related to prognosis patients TBM.Forty-five inpatients TBM in our institution from January 2013 December 2015 were enrolled retrospectively. The good or was defined, based on Glasgow Outcome Scale System discharge. Patients GOS score less than 5 defined as "poor prognosis." Univariate and multivariate logistic regression analyses...
Our objective is to study the clinical characteristics of cirrhosis patients with SIRS and investigate its prognostic factors.We analyzed 285 consecutive their data were evaluated retrospectively. Data compared in with/without during hospitalization. Univariate multivariate Cox regression analyses undertaken separately for cirrhotic assess predictive factors 90-day mortality.The mortality was 38.24% (52/136) 6.04% (9/149) without follow-up (P < 0.001). The univariate analysis showed...
Chronic hepatitis B (CHB) patients with higher virus (HBV) load (higher viral [HVL], HBV DNA ≥1 × 107 copies/mL) require antiviral therapy, but data for evaluating the long-term outcome of this therapy agents remain limited. We comparatively evaluated efficacy and safety nucleoside analogues in 179 noncirrhotic CHB HVL over 5 years. The HBeAg-positive (n = 104) or HBeAg-negative 75) were treated consecutively telbivudine (LdT, n 88) entecavir (ETV, 91) response, drug resistance safety. DNA,...
Drug- and herb-induced liver injury (DILI HILI) is an increasingly common serious condition. Here, data for DILI HILI patients from two large tertiary hospitals were retrospectively analyzed. Patient characteristics, causes severity of HILI, the correlation between expression p62 treatment prognostic factors studied. A total 82 with recruited study. Most presented hepatocellular injury, followed by cholestatic mixed injury. Our results indicate that traditional Chinese medicine or herbal...
// Yi-Ning Dai 1, * , Hai-Jun Huang Wen-Yuan Song 1 Yong-Xi Tong Dan-Hong Yang Ming-Shan Wang Yi-Cheng Mei-Juan Chen Jia-Jie Zhang Ze-Ze Ren 2 Wei Zheng and Hong-Ying Pan Department of Infectious Diseases, Zhejiang Provincial People's Hospital, Hospital Hangzhou Medical College, Hangzhou, Zhejiang, China The Second Affiliated Chinese Medicinal University, These authors have contributed equally to this work Correspondence to: Pan, email: hypanhz@139.com Keywords: tuberculous meningitis; viral...
Chronic hepatitis B (CHB) is a major global health burden. Liver fibrosis, an insidious process, the main histopathological change in CHB that might lead to end-stage liver disease if left untreated. The intermediate fibrosis (S2) optimal time start antiviral therapy. aim of present study was examine proteomic changes patients with at different fibrotic stages, view identify future serum biomarkers for S2. Ninety were grouped into mild (S0-1), (S2), and severe (S3-4) (61 men 29 women; age...
Abstract Background Severe fever and thrombocytopenia bunyavirus (SFTSV) infection causes severe syndrome with high mortality. It is extremely rare that a transmitting tick can be directly captured in bite wounds, SFTSV isolated from both the patient’s serum to establish solid pathogen diagnosis. Case presentation We report case infected bunyavirus. The 69-year-old male patient presented tenderness on two lymph nodes right groin. A visible mark appeared upper quadrant of abdomen, live was...
Abstract Novel coronavirus pneumonia (NCP) has been widely spread in China and several other countries. Early finding of this from huge numbers suspects gives clinicians a big challenge. The aim the study was to develop rapid screening model for early predicting NCP Zhejiang population, as well its utility areas. A total 880 participants who were initially suspected January 17 February 19 included. Potential predictors selected via stepwise logistic regression analysis. established based on...
Objective To investigate the changes and clinical significance of lipoxygenase-15, lipoxygenase-5, lipoxin A4, C-reactive protein (CRP) in patients with bacterial meningitis. Methods There were 104 divided into mild group (35 cases) , moderate (42 severe (27 40 normal healthy people selected as control group. The mRNA expressions lipoxygenase-15 lipoxygenase-5 detected by real time fluorescence quantitative reverse transcription-polymerase chain reaction. A4 level peripheral blood was...
Objective To analyze the relationship of expression peripheral blood T lymphocyte subsets (CD3+, CD4+ and CD8+ lymphocytes) regulatory cells (CD4+CD25+ Treg) with level HCV RNA in patients chronic hepatitis C(CHC). Methods According to RNA, 128 CHC cases were divide into negative group (n=48) , low virus (n=40, RNA<105 IU/mL) high (n= 40, ≥105 other 30 healthy persons selected as control. The lymphocytes 4 groups detected. differences analyzed among groups. Results The...
Objective To investigate the prognostic influence factors of liver cirrhosis patients with systemic inflammatory response syndrome (SIRS) . Methods A total 136 SIRS were analyzed retrospectively, and divided into death group (n=52) survival (n=84) by outcome disease. The clinical data in 2 groups compared. independent risk Logistic regression. Results The result single-factor analysis revealed that levels albumin (ALB) cholinesterase (CHE) (27.68±4.84) g/L (2 647.12±1 057.18)...
OBJECTIVE: To investigate the clinical significance of expression serum differential protein in patients with chronic hepatitis B (CHB) related liver fibrosis. METHODS: One hundred and ten CHB confirmed by biopsies were enrolled, 83 for modeling 27 verification. According to Ishak staging, 55 group significant fibrosis ( F is more than or equal 3 ) 28 normal/mild F0-F2 ). While that verification 15 12 ), respectively. MALDI-TOF-MS/MS was used detect proteins spectrum each sample analyzed...
Protein fingerprinting technology(PFF)is a novel technology for laboratory diagnosis developed in recent years.It has advantages of simple operation,testing quickly,high sensitivity and specificity.It is revolutional progress diagnosis.The application PFT medical field mainly the early detection diseases.PFT diagnostic with bright future potential applications.The review describes advances live disease diagnosis. Key words: Peptide mapping; Carcinoma,hepatocellar; Hepatic...
Objective To investigate the risk factors of liver histological change in chronic hepatitis B patients with low ALT level. Methods A total 321 level 2 times lower than upper limit normal (ULN) who accepted biopsy Zhejiang Provincial People's Hospital from January 2014 to September 2016 were enrolled, and divided into significant group (n=217) non-significant (n=104) . The clinical data including gender, age, WBC, function, HBeAg HBV DNA analyzed. Logistic regression was uesd screen...
Objective To understand the clinical characteristics of tuberculosis meningitis, and differential diagnosis features with other types meningitis such as viral cryptococcal purulent so to improve recovery rate this disease. Methods There were 45 patients 20 15 in Zhejiang Provincial People's Hospital from January 2013 December 2015 collected study. The compared those three meningitis. Results Tuberculosis commonly complicated pulmonary (29 cases, 64.4%) ; main underlying diseases...
Objective To evaluate the efficacy and safety of long-term nucleos(t)ide analogue treatment in patients with chronic hepatitis B (CHB). Methods Two hundred two initially treated CHB admitted Zhejiang Provincial People’s Hospital during March 2013 August 2016 were enrolled study. Patients divided into six groups according to different antiviral therapy: adefovir group (ADV, n=43), entecavir (ETV, n=44), lamivudine (LAM, n=25), telbivudine (LDT, n=23), LDT+ ADV (n=22), LAM+ (n=45). HBV...
Objective To assess the clinical characteristics and prognostic factors of bacterial liver abscess in patients with or without diabetes mellitus, to provide guides for diagnosis treatment. Methods A total 208 were enrolled during January 2014 2018 Zhejiang Provincial People’s Hospital, including 67 mellitus 141 mellitus. Clinical characteristics, laboratory examinntion, imaging findings, etiology results, treatment prognosis analyzed. Multiple logistic regression analysis was...
Abstract Novel coronavirus pneumonia (NCP) has been widely spread in China and several other countries. Early finding of this from huge numbers suspects gives clinicians a big challenge. The aim the study was to develop rapid screening model for early predicting NCP Zhejiang population, as well its utility areas. A total 880 participants who were initially suspected Jan 17 Feb 19 included. Potential predictors selected via stepwise logistic regression analysis. established based on...