A5100701788- Cancer Genomics and Diagnostics
- Glioma Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- vaccines and immunoinformatics approaches
- Dermatology and Skin Diseases
- Advancements in Transdermal Drug Delivery
- RNA Interference and Gene Delivery
- Analytical Methods in Pharmaceuticals
- Infant Nutrition and Health
- Computational Drug Discovery Methods
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Chromatography
- Electrochemical sensors and biosensors
- Pharmacological Effects and Assays
- Lung Cancer Treatments and Mutations
- Genomics and Rare Diseases
- Cardiac, Anesthesia and Surgical Outcomes
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Sensors
- Nanoparticle-Based Drug Delivery
Peter MacCallum Cancer Centre
2016-2024
Patna University
2021-2024
Monash Health
2022-2023
National Dairy Research Institute
2023
Rajendra Institute of Medical Sciences
2009-2023
The University of Melbourne
2018-2022
Monash Medical Centre
2022
Children's Cancer Institute Australia
2018-2021
UNSW Sydney
2018-2021
Royal Children's Hospital
2021
Micro RNAs (miRNAs) are a class of small, non-coding RNA species that play critical roles throughout cellular development and regulation. miRNA expression patterns taken from various tissue types often point to the lineage an individual type, thereby being more invariant hallmark type. Recent work has shown these can be used classify tumor cells, this classification accurate than achieved by using messenger gene patterns. One aspect biogenesis makes them particularly attractive as biomarker...
Genes encoding TRK are oncogenic drivers in multiple tumour types including infantile fibrosarcoma, papillary thyroid cancer and high-grade gliomas (HGG). fusions have a critical role tumourigenesis 40% of infant HGG. Here we report the first case fusion-driven HGG treated with larotrectinib—the selective pan-TRK inhibitor clinical development. This 3-year-old girl had failed therapies chemotherapy radiotherapy. Tumour profiling confirmed an ETV6–NTRK3 fusion. Treatment larotrectinib led to...
The recent dramatic appearance of variants concern SARS-coronavirus-2 (SARS-CoV-2) highlights the need for innovative approaches that simultaneously suppress viral replication and circumvent escape from host immunity antiviral therapeutics. Here, we employ genome-wide computational prediction single-nucleotide resolution screening to reprogram CRISPR-Cas13b against SARS-CoV-2 genomic subgenomic RNAs. Reprogrammed Cas13b effectors targeting accessible regions Spike Nucleocapsid transcripts...
Lactation is a key aspect of mammalian evolution for adaptation various reproductive strategies along different lineages. Marsupials, such as tammar wallaby, adopted short gestation and relatively long lactation cycle, the newborn immature at birth significant development occurs postnatally during lactation. Continuous changes milk composition may contribute to immune protection pouch young. Here, in order address putative contribution newly identified secretory miRNA these processes, high...
Topical 5-fluorouracil (5-FU) is approved for the treatment of superficial basal cell carcinoma and actinic keratosis. However, 5-FU suffers from poor skin permeation. Microneedles have been successfully applied to improve permeability small large molecules, even nanoparticles, by creating micron-sized pores in stratum corneum layer skin. In this report, feasibility using microneedles increase was tested. Using full thickness mouse mounted on Franz diffusion apparatus, it shown that flux...
Deregulated expression of MYC induces a dependence on the NUAK1 kinase, but molecular mechanisms underlying this have not been fully clarified. Here, we show that is predominantly nuclear protein associates with network phosphatase 1 (PP1) interactors and PNUTS, regulatory subunit PP1, phosphorylated by NUAK1. Both PNUTS associate splicing machinery. Inhibition abolishes chromatin association reduces spliceosome activity, suppresses nascent RNA synthesis. Activation does bypass requirement...
microRNAs are the post-transcriptional regulators implicated in initiation and progression of various cancer types, including oral squamous cell carcinoma (OSCC). Here, we investigated role miR-377 OSCC tumorigenesis. expression was reduced samples line (UPCI-SCC-116), associated with patient survival. In vitro restoration repressed growth, induced apoptosis, migration. We identified HDAC9 as a target found to regulate its pro-apoptotic target, NR4A1/Nur77. Our findings show that targets...
Background: The present study was designed to evaluate the extent which pretreatment with microneedles can enhance skin permeation of nanoparticles in vitro and vivo. Permeation live bacteria, are physically or microparticles, through mouse pretreated also studied potential risk microbial infection. Methods results: It found that allowed solid lipid nanoparticles, size 230 nm, ovalbumin conjugated on their surface. Transcutaneous immunization a area induced stronger antiovalbumin antibody...
Context: Facial hirsutism is a cosmetic concern for women and can lead to significant anxiety lack of self-esteem. Eflornithine cream indicated the treatment facial hirsutism. However, limited success rate overall patient's satisfaction, even with long-term high-frequency application, leave room improvement.Objective: The objective this study test effect microneedle on in vitro skin permeation vivo efficacy eflornithine mouse model.Materials method: In was performed using Franz diffusion...
Abstract Gefitinib, an EGFR tyrosine kinase inhibitor, is used as FDA approved drug in breast cancer and non-small cell lung treatment. However, this has certain side effects complications for which the underlying molecular mechanisms are not well understood. By systems biology based silico analysis, we identified off-targets of gefitinib that might explain drugs. The crystal structure EGFR-gefitinib complex was binding pocket similarity searches on a druggable proteome database (Sc-PDB) by...
Abstract The development of precise RNA-editing tools is essential for the advancement RNA therapeutics. CRISPR (clustered regularly interspaced short palindromic repeats) PspCas13b a programmable nuclease predicted to offer superior specificity because its 30-nucleotide spacer sequence. However, design principles and on-target, off-target collateral activities remain poorly characterized. Here, we present single-base tiled screening computational analyses that identify key potent highly...
The increasing affordability of DNA sequencing has allowed it to be widely deployed in pathology laboratories. However, this exposed many issues with the analysis and reporting variants for clinical diagnostic use. Implementing a high-throughput (NGS) system requires diverse combination capabilities, statistical methods identify variants, global variant databases, validated bioinformatics pipeline, an auditable laboratory workflow, reproducible assays quality control monitoring throughout....
Co-therapy through biotin modified nanoparticles (NPs) of gefitinib (Gnb) and naringenin (Nar) was investigated for its therapeutic synergistic potential against lung cancer. The biotin-conjugated polymeric NPs (bty-Nar/Gnb) were developed using oil in water emulsion technique optimized central composite design. formulations subjected to various vitro (A549 cell lines) vivo evaluations urethane-induced Co-administration Gnb Nar displayed a significant reduction tumour volume while restoring...