A5048813972- Mesenchymal stem cell research
- Bone fractures and treatments
- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- Orthopaedic implants and arthroplasty
- Cell Adhesion Molecules Research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Knee injuries and reconstruction techniques
- Bone Metabolism and Diseases
- Total Knee Arthroplasty Outcomes
- 3D Printing in Biomedical Research
- Cancer-related gene regulation
- Tendon Structure and Treatment
- Connective tissue disorders research
- Wound Healing and Treatments
- Shoulder Injury and Treatment
- Silk-based biomaterials and applications
- Cancer, Hypoxia, and Metabolism
- Orthopedic Infections and Treatments
- Assisted Reproductive Technology and Twin Pregnancy
- RNA Interference and Gene Delivery
- Hematopoietic Stem Cell Transplantation
- Bone health and osteoporosis research
Clinica Universidad de Navarra
2014-2025
Navarre Institute of Health Research
2018-2025
Universidad de Navarra
2012-2023
Instituto de Investigación Biosanitaria de Granada
2020
Shanghai Cell Therapy Research Institute
2016
University of North Carolina at Chapel Hill
2008-2012
Vanderbilt University Medical Center
2008-2011
Centro de Investigacion Principe Felipe
2005-2011
Weatherford College
2008
Vanderbilt University
2006-2007
Abstract Mesenchymal stem cells (MSC) have a therapeutic potential in patients with fractures to reduce the time of healing and treat nonunions. The use MSC is attractive for several reasons. First, MSCs would be implementing conventional reparative process that seems defective or protracted. Secondly, effects treatment needed only relatively brief duration reparation. However, an integrated approach define multiple regenerative contributions fracture repair necessary before clinical trials...
Mesenchymal stromal cells are a promising option to treat knee osteoarthritis. Their safety and usefulness must be confirmed the optimal dose established. We tested increasing doses of bone marrow mesenchymal (BM-MSCs) in combination with hyaluronic acid randomized clinical trial.A phase I/II multicenter trial active control was conducted. Thirty patients diagnosed OA were randomly assigned intraarticularly administered alone (control), or together 10 × 10(6) 100 cultured autologous BM-MSCs,...
Despite its clinical significance, joint morphogenesis is still an obscure process. In this study, we determine the role of transforming growth factor β (TGF-β) signaling in mice lacking TGF-β type II receptor gene (Tgfbr2) their limbs (Tgfbr2PRX-1KO). Tgfbr2PRX-1KO mice, loss responsiveness resulted absence interphalangeal joints. The Tgfbr2Prx1KO phenotype similar to that patients with symphalangism (SYM1-OMIM185800). By generating a Tgfbr2–green fluorescent protein–β–GEO–bacterial...
Abstract Background Mesenchymal stromal cells are a safe and promising option to treat knee osteoarthritis as previously demonstrated in different clinical trials. However, their efficacy, optimal dose addition of adjuvants must be determined. Here, we evaluated the effects 100 × 10 6 bone marrow mesenchymal (BM-MSCs) combination with Platelet Rich Plasma (PRGF®) adjuvant randomized trial. Methods A phase II, multicenter, trial active control was conducted. Sixty patients diagnosed OA were...
Failures of fracture repair (nonunions) occur in 10% all fractures. The use mesenchymal stem cells (MSC) tissue regeneration appears to be rationale, safe, and feasible. contributions MSC the reparative process can through autocrine paracrine effects. primary objective this study is find a novel mean, by transplanting cultures bone marrow-derived MSCs expressing insulin-like growth factor-I (MSC(IGF)), promote these seed-and-soil actions fully implement their regenerative abilities...
The aim of this study was to assess a novel approach treating severe knee osteoarthritis by targeting synovial membrane, superficial articular cartilage, fluid, and subchondral bone combining intra-articular injections intraosseous infiltrations platelet rich plasma. We explored new strategy consisting plasma into the in combination with conventional injection order tackle several joint tissues simultaneously. assessed clinical outcomes through outcome score (KOOS) inflammatory response...
Biopolymers play a critical role as scaffolds used in tendon and ligament (TL) regeneration. Although advanced biopolymer materials have been proposed with optimised mechanical properties, biocompatibility, degradation, processability, it is still challenging to find the right balance between these properties. Here, we aim develop novel hybrid biocomposites based on poly(p-dioxanone) (PDO), poly(lactide-co-caprolactone) (LCL) silk produce high-performance grafts suitable for TL tissue...
The interface tissue between bone and soft tissues, such as tendon ligament (TL), is highly prone to injury. Although different biomaterials have been developed for TL regeneration, few address the challenges of TL-bone interface. Here, we aim develop novel hybrid nanocomposites based on poly(p-dioxanone) (PDO), poly(lactide-co-caprolactone) (LCL), hydroxyapatite (HA) nanoparticles suitable repair. Nanocomposites, containing 3-10% both unmodified chemically modified (mHA) with a silane...
Background: Bone marrow mesenchymal stem cell (BM-MSC) therapy has emerged as a safe and feasible treatment option for patients with knee osteoarthritis (OA). However, the role of adjuvants remains unclear. Our aim was to evaluate clinical radiological effects hyaluronic acid (HA) in comparison platelet-rich plasma (PRP) 100 × 106 BM-MSCs OA. Methods: We used data from two randomized, parallel-group controlled trials which tested efficacy BM-MSC, previously published 2016 (Clinical...
An integral approach toward in situ tissue engineering through scaffolds that mimic with regard to both architecture and biochemical composition is presented. Monolithic osteochondral meniscus are prepared analog layered perpendicularly oriented continuous micropores by a newly developed cryostructuring technology. These enable rapid cell ingrowth induce zonal-specific matrix synthesis of human multipotent mesenchymal stromal cells solely their design without the need for supplementation...
During bone regeneration, the periosteum acts as a carrier for key regenerative cues, delivering osteochondroprogenitor cells and crucial growth factors to injured bone. We developed biocompatible, 3D polycaprolactone (PCL) melt electro-written membrane act mimetic periosteum. Poly (ethyl acrylate) coating of PCL allowed functionalization, mediated by fibronectin low dose recombinant human BMP-2 (rhBMP-2) (10-25 μg/ml), resulting in efficient, sustained osteoinduction vitro. In vivo, rhBMP-2...
The non-collagenous C-terminal domain of the α<sub>3</sub> chain collagen IV is autoantigen in Goodpasture disease, an autoimmune disorder described only humans. Specific N-terminal phosphorylation a biological feature unique to human when compared with other homologous domains lacking immunopathogenic potential. We have recently cloned from HeLa-derived cDNA library novel serine/threonine kinase (Goodpasture antigen-binding protein (GPBP)) that phosphorylates region (Raya, A. Revert, F,...
Abstract Fracture nonunion is a major complication of bone fracture regeneration and repair. The molecular mechanisms that result in appearance are not fully determined. We hypothesized results from the failure hypoxia hematoma, primary signals response to injury, trigger Bmp2 expression by mesenchymal progenitor cells (MSCs). Using model nonstabilized healing transgenic 5′Bmp2BAC mice we determined appears close association with hypoxic tissue hematoma during early phases healing. In...
In this study, we examined the effectiveness of systemic subcutaneous delivery recombinant Insulin-like growth factor (IGF)-I concurrently with primary cultured bone marrow-derived mesenchymal stem cell (MSC) transplant on fracture repair. We found that callus volume increased in mice a stabilized tibia received IGF-I+MSC when compared either untreated or MSC alone treated mice. evaluating tissue components, soft and new volumes were significantly recipients. Histological in-situ...
The aim of this study was to evaluate the effect intra‐articular (IA) or a combination and intraosseous (IO) infiltration Platelet Rich Plasma (PRP) on cellular content synovial fluid (SF) osteoarthritic patients. Thirty‐one patients received single PRP either in IA space ( n = 14) together with two IO infiltrations, one medial femoral condyle tibial plateau 17). SF collected before after week infiltration. presence mesenchymal stem cells (MSCs), monocytes, lymphocytes determined quantified...
Human Goodpasture antigen-binding protein (GPBP) is an atypical kinase that phosphorylates the auto-antigen, alpha3 chain of collagen IV. The COL4A3BP gene alternatively spliced producing two isoforms: GPBP and GPBPDelta26. latter lacks a serine-rich domain composed 26 amino acid residues. Both isoforms also function as ceramide transfer proteins (CERT). Here, we explored Gpbp GpbpDelta26/CERT during embryogenesis in zebrafish. We cloned both splice variants zebrafish found they are...
An attractive alternative to bone autografts is the use of autologous mesenchymal progenitor cells (MSCs) in combination with biomaterials. We compared therapeutic potential different sources stem biomaterials a nonunion model. A critical-size defect was created Sprague-Dawley rats. Animals were divided into six groups, depending on treatment be applied: left empty (CTL); treated live allograft (LBA); hrBMP-2 collagen scaffold (CSBMP2 ); acellular polycaprolactone (PCL group); PCL containing...
Goodpasture-antigen binding protein (GPBP) is a nonconventional Ser/Thr kinase for basement membrane type IV collagen. Various studies have questioned these findings and proposed that GPBP serves as transporter of ceramide between the endoplasmic reticulum Golgi apparatus. Here we show cells expressed at least two isoforms resulting from canonical (77-kDa) noncanonical (91-kDa) mRNA translation initiation. The 77-kDa polypeptide interacted with collagen localized soluble form in...
Bone marrow derived mesenchymal stem cells (BM-MSC) can differentiate into chondrocytes. Understanding the mechanisms and growth factors that control MSC stemness is critical to fully implement their therapeutic use in cartilage diseases. The activated type 1 insulin-like factor receptor (IGF-IR), interacting with insulin substrate-1 (IRS-1), induce cancer cell proliferation transformation. In or transformed cells, IRS-1 has been shown localize cytoplasm where it activates canonical Akt...
In the treatment of bone non-unions, an alternative to autografts is use morphogenetic proteins (BMPs), e.g., BMP–2, BMP–7, with powerful osteoinductive and osteogenic properties. clinical settings, these factors are applied using absorbable collagen sponges for local controlled delivery. Major side effects this strategy derived from supraphysiological doses BMPs needed, which may induce ectopic formation, chronic inflammation, excessive resorption. order increase efficiency delivered BMPs,...