A5015356505- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Cell Adhesion Molecules Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Atherosclerosis and Cardiovascular Diseases
- Immune cells in cancer
- RNA Research and Splicing
- Thyroid Cancer Diagnosis and Treatment
- Micro and Nano Robotics
- Pharmacological Effects and Assays
- Nuclear Receptors and Signaling
- Enzyme function and inhibition
- Electrochemical sensors and biosensors
- Macrophage Migration Inhibitory Factor
- Medical Imaging and Pathology Studies
- Multiple Myeloma Research and Treatments
- Cancer-related gene regulation
- Virus-based gene therapy research
- Hippo pathway signaling and YAP/TAZ
- Molecular Communication and Nanonetworks
Shanghai Jiao Tong University
2021-2025
Shanghai First People's Hospital
2021-2025
First Affiliated Hospital of Bengbu Medical College
2024
Panyu District Central Hospital
2014-2020
Third Xiangya Hospital
2017-2020
Central South University
2017-2020
Capital Medical University
2008-2010
Abstract Osteosarcoma (OS) is a primary malignant bone tumor that most commonly affects children, adolescents, and young adults. Here, we comprehensively analyze genomic, epigenomic transcriptomic data from 121 OS patients. Somatic mutations are diverse within the cohort, only TP53 significantly mutated. Through unsupervised integrative clustering of multi-omics data, classify into four subtypes with distinct molecular features clinical prognosis: (1) Immune activated (S-IA), (2) suppressed...
Information processing tools and bioinformatics software have significantly advanced researchers' ability to process analyze biological data. Molecular data from human model organism genomes help researchers identify topics for study, which, in turn, improves predictive accuracy, facilitates the identification of relevant genes, simplifies validation laboratory The objective this study was explore regulatory network constituted by long noncoding RNA (lncRNA), miRNA, mRNA prostate cancer...
Prostate cancer gene expression marker 1 (PCGEM1) is a long non-coding RNA (lncRNA) overexpressed in prostate (PCa) cells that promotes PCa initiation and progression, protects against chemotherapy-induced apoptosis. The microRNA miR-145 functions as tumor suppressor PCa. We speculate reciprocal regulation of PCGEM1 promote proliferation LNCaP cells. To test this hypothesis, the interaction between was examined using luciferase reporter assay. Expression levels were selectively altered...
Osteosarcoma (OS), a malignant bone tumor with limited treatment options, exhibits low sensitivity to immune checkpoint therapy (ICT). Through genomics and transcriptomics analyses, we identify subgroup of OS methylthioadenosine phosphorylase (MTAP) deletion, which contributes ICT resistance, leading "cold" microenvironment. MTAP-deleted relies on methionine metabolism is sensitive intervention, achieved through either dietary restriction or inhibition adenosyltransferase 2a (MAT2A), key...
circRNA, a type of RNA that widely expressed in the mammalian cells, is considered to be essential tumorgenesis. circRNA can regulate target gene expression by interacting with corresponding microRNA (miRNA). Our preliminary results showed levels 1817 circRNAs were significantly different colon cancer tissue, among which 1236 them up-regulated and 581 down-regulated. By using RT-PCR, we confirmed expressions hsa_circ_0007843, hsa_circ_0010575, hsa_circ_0007331 hsa_circ_0001615 obviously...
Rap1GAP expression is decreased in human tumors. The significance of its downregulation unknown. We show that primary colorectal carcinomas. To elucidate the advantages conferred on tumor cells by loss Rap1GAP, was silenced colon carcinoma cells. Suppressing induced profound alterations cell adhesion. Rap1GAP-depleted exhibited defects cell/cell adhesion included an aberrant distribution adherens junction proteins. Depletion enhanced and spreading collagen. Silencing Rap normalized restored...
Rap1 GTPase-activating protein (GAP) regulates the activity of Rap1, a putative oncogene. We previously reported Rap1GAP was highly expressed in normal human thyroid cells and decreased five papillary carcinomas (PTCs).To confirm significance these findings, we analyzed expression larger set benign tumors (adenomas hyperplastic nodules) PTCs. determined whether presence BRAF(V600E) mutation or allelic loss related to changes expression. To determine consequences loss, targeted culture using...
Abstract Osteosarcoma (OS) exhibit intra- and inter-heterogeneity, complicating the exploration of effective therapeutic strategies. Traditional in vitro vivo models are limited inheriting biological genomic heterogeneities OS patients, even features on tumor microenvironment. The prolonged generation time current makes drug development slow is not suitable to clinically rapid timing. Here, we introduce methods for generating biobanking patient/PDX-derived osteosarcoma organoids (OS PD(X)Os)...
Osteosarcoma is the most common primary malignant bone tumor, and there are few ideal clinically available drugs. The bromodomain extraterminal domain (BET) protein an emerging target for aggressive cancer, but therapies targeting BET in osteosarcoma have been unsuccessful clinical trials to date, further exploration of specific inhibitors great significance. In our study, we demonstrated that NHWD-870, a potent inhibitor phase I trial, significantly inhibited tumor proliferation promoted...
Multi-cause-induced interstitial lung disease, particularly pulmonary fibrosis, is a serious clinical concern. Fibroblasts have been suggested to major role, with it recently being revealed that some of these fibroblasts are derived from alveolar epithelial cells through epithelial-mesenchymal transition (EMT). Eukaryotic translation initiation factor 3 subunit A (EIF3A) protein in humans encoded by the EIF3A gene, and has play roles regulating subset mRNAs cell cycle progression...
Prostate cancer gene expression marker 1 (PCGEM1) is a long non-coding RNA (lncRNA) overexpressed in prostate (PCa) cells that promotes PCa initiation and progression, protects against chemotherapy-induced apoptosis. The microRNA miR-145 functions as tumor suppressor PCa. We speculate reciprocal regulation of PCGEM1 promote proliferation LNCaP cells. To test this hypothesis, the interaction between was examined using luciferase reporter assay. Expression levels were selectively altered...
The inflammatory response and oxidative stress play key roles in the formation development of atherosclerosis. Bazedoxifene is a new IL6/GP130 inhibitor recommended by FDA for clinical use as selective estrogen receptor modulator. However, its role cardiovascular diseases has been poorly studied. In our study, we explored mechanism bazedoxifene’s protective effect against injury vascular endothelial cells (VECs) stimulated TNF-α. Various methods were used to verify bazedoxifene on VECs,...
Atherosclerosis (AS) is one of the leading causes death, with variable frequencies in different populations. To this end, we examined whether AS associated miR-145 rs353291 polymorphism and specific alleles. The distribution C allele CC genotypes was significantly higher patients than controls. Compared to individuals TT genotype, carriers showed a significant increase plasma levels sCD40L, IL-6 MCP-1 decrease expression. After ox-LDL administration, peripheral blood mononuclear cells...
<title>Abstract</title> AXL has ubiquitous expression in multiple cancers, and is strongly linked to both tumor progression, metastasis, poor prognosis, as well anti-tumor immune response suppression induction of resistance immunotherapy. Therefore, it a strong target for cancer intervention. Despite the wide application inhibitors clinical trials, role microenvironment (TIME) remains undetermined. Herein, we established cell lines with stable knockdown or overexpression using lentiviral...
Conclusions:We demonstrated the novel finding of a direct promoting effect high cellular insulin or glucose exposure on homocysteine remethylation, MAT activity, and adoMet synthesis.We also provided new evidence indicating that when hepatic tissue is exposed to elevated glucose, methylation balance can be altered, which may have potential epigenetic impacts gene regulation in diabetic individuals.These findings cell-line not reflect what happens humans.In vivo studies transmethylation...