A5102894611- Sarcoma Diagnosis and Treatment
- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Cancer, Hypoxia, and Metabolism
- Mechanisms of cancer metastasis
- Cancer Research and Treatments
- Management of metastatic bone disease
- Protein Hydrolysis and Bioactive Peptides
- Bone Tumor Diagnosis and Treatments
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- Soft tissue tumor case studies
- Orthopaedic implants and arthroplasty
- Lung Cancer Research Studies
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Pelvic and Acetabular Injuries
- Phytochemical compounds biological activities
- Bone health and treatments
- Porphyrin and Phthalocyanine Chemistry
- Neuroblastoma Research and Treatments
- Radiation Therapy and Dosimetry
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Peptidase Inhibition and Analysis
Shanghai First People's Hospital
2016-2025
Shanghai Jiao Tong University
2015-2025
Jilin Agricultural University
2025
Shanghai Cancer Institute
2019
Shanghai First Maternity and Infant Hospital
2019
Shanghai Stomatological Hospital
2017
Tongji University
2011-2016
Shanghai Tenth People's Hospital
2011-2016
Hebei North University
2015
Nanjing Medical University
2014
Osteosarcoma is the most common primary bone cancer and notorious for pulmonary metastasis, representing a major threat to pediatric patients. An effective drug targeting osteosarcoma its lung metastasis urgently needed.In this study, sarcoma-targeting peptide-decorated disulfide-crosslinked polypeptide nanogel (STP-NG) was exploited enhanced intracellular delivery of shikonin (SHK), an extract medicinal herb, inhibit progression with minimal systemic toxicity.The targeted, loaded nanogel,...
Osteosarcoma is the most common primary malignant bone tumor in children and adolescents, with highly aggressive behavior early systemic metastasis. The survival rates for osteosarcoma remain unchanged over past two decades. Studies aiming to find new or alternative therapies patients refractory are urgently needed. Anlotinib, a novel multi‐targeted tyrosine kinase inhibitor (TKI), has exhibited encouraging clinical activity NSLCC soft tissue sarcoma, whereas its effect on not been studied....
Abstract Osteosarcoma (OS) is a primary malignant bone tumor that most commonly affects children, adolescents, and young adults. Here, we comprehensively analyze genomic, epigenomic transcriptomic data from 121 OS patients. Somatic mutations are diverse within the cohort, only TP53 significantly mutated. Through unsupervised integrative clustering of multi-omics data, classify into four subtypes with distinct molecular features clinical prognosis: (1) Immune activated (S-IA), (2) suppressed...
Background/Aims: Over the past decade, heat shock protein 90 (Hsp90) has emerged as a potential therapeutic target for cancer. However, molecular mechanisms of down-regulation Hsp90 expression in osteosarcoma are incompletely understood. To develop therapy targeting Heat 90B1 (Hsp90B1), we studied roles miR- 223 proliferation and apoptosis human osteosarcoma. Methods: pcDNA3.1(+)- miR-223 plasmid vectors were constructed transfected into MG63 cells. Co-transfection vector with pMIR-Hsp90B1...
Osteosarcoma (OS), a malignant bone tumor with limited treatment options, exhibits low sensitivity to immune checkpoint therapy (ICT). Through genomics and transcriptomics analyses, we identify subgroup of OS methylthioadenosine phosphorylase (MTAP) deletion, which contributes ICT resistance, leading "cold" microenvironment. MTAP-deleted relies on methionine metabolism is sensitive intervention, achieved through either dietary restriction or inhibition adenosyltransferase 2a (MAT2A), key...
Osteosarcoma (OS) is the most common primary malignant tumor of bone in children and adolescents. However, knowledge diagnostic modalities has progressed less. To identify new biomarkers for early diagnosis OS as well potential novel therapeutic candidates, we performed a sub-cellular comparative proteomic research.An osteosarcoma cell line (MG-63) human osteoblastic cells (hFOB1.19) were used our model. Plasma membrane (PM) was obtained by aqueous two-phase partition. Proteins analyzed...
This study was carried out to investigate the anti-tumor effect and mechanism of hiporfin-mediated photodynamic therapy (hiporfin-PDT) in osteosarcoma. We found that hiporfin accumulated mainly cytoplasm osteosarcoma cells a time concentration-dependent manner. Hiporfin-PDT inhibited proliferation, induced apoptosis produced cell cycle arrest at G2M lines. increased expression cleaved-caspase-3, cleaved PARP-1, Bax RIP1 while it decreased Bcl-2; addition, low concentration LC3 conversion....
Osteosarcoma (OS) is the most frequent primary malignant bone tumour. Alternol, a novel compound purified from microbial fermentation products exerts anti-tumour effects across several cancer types. The effect of alternol on human OS remains to be elucidated. We first evaluated in cell lines vitro and investigated its underlying mechanism. Alternol inhibited proliferation, migration induced caspase-dependent apoptosis, G2/M cycle arrest dose time-dependent manner. Moreover, treatment signal...
Two-color stimulated Raman scattering (SRS) microscopy with label-free mapping of lipid/protein distributions has shown promise in virtual histology.Despite previous demonstrations SRS tumor delineation and diagnosis, the speed efficiency current technique requires further improvements for practical use.Here, we integrate parallel dual-phase detection strip mosaicing, which reduces imaging time a whole mouse brain section from 70 to 8 minutes.We verified our method fresh human surgical...
Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. To identify new biomarkers for early diagnosis of OS novel therapeutic candidates, we carried out a plasma membrane proteomic study based on two-dimensional electrophoresis (2DE). The cell line MG-63 human osteoblastic hFOB1.19 were adopted as comparison model. We extracted by aqueous two-phase partition extraction. proteins separated through 2DE. analyzed differentially expressed Imagemaster...
We investigated the antitumor effect and mechanism of hematoporphyrin monomethyl ether-mediated photodynamic therapy (HMME-PDT) in sarcomas. Intracellular uptake HMME by osteosarcoma cells (LM8 K7) was time- dose-dependent, while this not observed for myoblast (C2C12) fibroblast (NIH/3T3). HMME-PDT markedly inhibited proliferation sarcoma cell lines (LM8, MG63, Saos-2, SW1353, TC71, RD) (P<0.05), killing improved with increased concentration energy intensity. Flow cytometry analysis revealed...
Abstract Combretastatin A-4 (CA-4), a tubulin-depolymerizing agent, shows promising antitumor efficacy and has been under several clinical trials in solid tumors for 10 years. Autophagy an important pro-survival role cancer therapy, thus targeting autophagy may improve the of agents. N-myc downstream-regulated gene 1 (NDRG1) is significant stress regulatory gene, which mediates cell survival chemoresistance. Here we reported that CA-4 could induce cell-protective autophagy, combination...
Abstract Osteosarcoma (OS) exhibit intra- and inter-heterogeneity, complicating the exploration of effective therapeutic strategies. Traditional in vitro vivo models are limited inheriting biological genomic heterogeneities OS patients, even features on tumor microenvironment. The prolonged generation time current makes drug development slow is not suitable to clinically rapid timing. Here, we introduce methods for generating biobanking patient/PDX-derived osteosarcoma organoids (OS PD(X)Os)...
The gelsolin (GSN) has been involved in the regulation of tumor formation and development, but biological role GSN pathogenesis osteosarcoma (OS) not well characterized. In this study, we show that high expression was observed OS tissues correlated with size, advanced Enneking stage, poor patient prognosis. Knockdown significantly inhibited cell proliferation invasiveness lines. Furthermore, stable overexpression cells resulted a significant increase growth motility downregulation p-AKT...
// Zhuoying Wang 1, * , Chongren Zifei Zhou Mengxiong Sun 1 Chenghao 2 Jian Chen Fei Yin Hongsheng Binhui Lin Dongqing Zuo Suoyuan Li Lijin Feng 3 Zhenfeng Duan 4 Zhengdong Cai Yingqi Hua Department of Orthopedics, Shanghai General Hospital, Jiao Tong University School Medicine, Shanghai, 200080, China Bone Tumor Institution, 201620, Pathology, Tenth People’s Tongji 200072, Sarcoma Biology Laboratory, Orthopaedic Surgery, Massachusetts Hospital and Harvard Medical School, Boston, MA,...