A5072232284- Probiotics and Fermented Foods
- Gut microbiota and health
- Protein Hydrolysis and Bioactive Peptides
- Biochemical effects in animals
- HIV Research and Treatment
- Antimicrobial Peptides and Activities
- Herpesvirus Infections and Treatments
- Meat and Animal Product Quality
- Biosensors and Analytical Detection
- Advanced Chemical Sensor Technologies
- Food Quality and Safety Studies
- Microbial Metabolites in Food Biotechnology
- Poxvirus research and outbreaks
- Muscle metabolism and nutrition
- Microbial Metabolism and Applications
- Animal Nutrition and Physiology
- Antibiotic Resistance in Bacteria
- Fermentation and Sensory Analysis
- Diet and metabolism studies
- vaccines and immunoinformatics approaches
- Biochemical Analysis and Sensing Techniques
- Listeria monocytogenes in Food Safety
- Polyamine Metabolism and Applications
- Phytochemicals and Antioxidant Activities
- Virus-based gene therapy research
Jilin Agricultural University
2016-2025
University of Technology
2018
National Engineering Research Center for Wheat
2015-2018
Academy of Military Medical Sciences
2010-2017
Jilin University
2010-2012
Objective. Lactic acid bacteria (LAB) were isolated from fermented foods, such as glutinous rice dough, corn noodle, chili sauce, potherb mustard pickles, and stinky tofu, in northeast China. LAB strains with antimicrobial activities screened, seven of these Lactobacillus identified L. plantarum , pentosus paracasei through 16S rRNA gene analysis. After the supernatant was treated proteinase K, pepsin, papain, their antibacterial effect almost disappeared. Most highly resistant to heat...
Walnut protein, which is obtained as a by-product of oil expression, has not been used efficiently. Although walnuts are beneficial for cognitive functioning, the potential their protein composition in strengthening learning and memory functions remains unknown. In this study, inhibition impairment by Manchurian walnut hydrolyzed peptide (MWHP) was evaluated.Small-molecular-weight MWHP (<3 kDa) achieved optimal antioxidative activity. Therefore, subjected to following mice trials evaluate...
Evidence suggests that probiotic interventions reduce non-communicable diseases (NCDs) risk. However, its therapeutic effect and mechanism are still unclear. To evaluate the hypocholesterolemic of Lactobacillus plantarum H6 (L.p H6), a new commercial patent strain capable preventing hypercholesterolemia, in depth, three states were prepared, namely, viable (vH6), heat-inactivated (iH6), ultrasonically-lysed (uH6) bacteria cells. The results showed v/i/uH6 cells could lower serum liver blood...
The probiotic properties of two selected lactobacilli strains were assessed. L. salivarius and plantarum displayed higher hydrophobicity (48% 54%, resp.) coaggregation ability with four pathogens (from 7.9% to 57.5%). had good inhibitory effects on S. aureus (38.2% 49.5%, attachment Caco-2 cells. Live their conditioned media effectively inhibited IL-8 production (<14.6 pg/mL) in TNF- α -induced Antibiotic-treated the sonicated also maintained (IL-8 from 5.0 36.3 pg/mL); however,...
The quality of gray sufu has been significantly influenced by microbes, but little is known about the spatiotemporal changes in microbes during fermentation and how they relate to quality. This study showed that after 28 days fermentation, texture physicochemical indexes reached best, taste was better on 21st day. On surface sufu, abundance Bacillus Trichosporon increased over time, while Lactobacillus, Pediococcus, Pseudomonas decreased. In middle central parts, Lactobacillus Sarocladium...
In addition to providing certain health advantages the host, a bacterial strain must possess clearly defined safety profile be regarded as probiotic. this study, we present thorough and methodical assessment of novel bacteria, Akkermansia muciniphila PROBIO, which was isolated from human feces. Firstly, examined strain’s overall features, such its gastrointestinal tolerance physiological biochemical traits. Next, verified genotoxic properties through reverse mutation in vitro mammalian cell...
Hypercholesterolemia is the leading cause of cardiovascular disease worldwide. In this study, lactic acid bacteria (Lactobacillus plantarum H6) screened from homemade fermented foods in northeastern China were used to study their cholesterol-lowering ability and analyze degradation mechanism. Results showed that L. H6 significantly reduced serum cholesterol levels C57BL/6 mice fed a hypercholesterolemia diet. It mainly promoted expression CYP7A1 gene by inhibiting farnesoid X receptor...
The applicability of a new strain, Weissella confusa M1, as an adjunct culture for gray sufu was investigated the first time from microbial and flavor perspectives. Results showed that M1 inoculation improved flavor, color, texture, taste properties compared with natural fermentation. increased contents 13 free amino acids, especially aspartate glutamate, drove diversity dynamics community during fermentation by being involved in transport metabolism acids carbohydrates. Many volatile...
Gray sufu is a traditional fermented bean product with strong flavor in China, but fermentation methods often lead to its off-flavor. This study was performed investigate the quality characteristics of gray using L. mesenteroides F24. Results showed 220 volatile compounds sufu, among which alcohols and esters were main volatiles. Inoculation F24 considerably affected contents substances substantially increased compounds. In addition, 29 kinds key identified by analyzing ROAVs. Four unique...
Context: Antidiabetic activity of Coptis chinensis Franch (Ranunculaceae) polysaccharide (CCPW) has been reported. However, its molecular mechanism remains unclear.Objective: An attempt was made to further verify the antidiabetic CCPW on type 2 diabetes mellitus (T2DM) and elucidate activity.Materials methods: Male Wistar rats were fed with high-fat diet (HFD) injected streptozotocin (STZ) generate a T2DM model. Effects fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC),...
We co-cultured Caco-2 cells with Lactobacillus salivarius and L. plantarum in vitro to investigate their immunoregulatory mechanisms detected strains adhering expressing mRNAs of cytokines Toll-like receptors (TLRs) by fluorescent quantitative reverse transcription–polymerase chain reaction. The were intragastrically administered mice. Thymus/spleen indexes measured on different days. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay, flow cytometry, enzyme-linked...
Probiotics have been considered a promising option for mitigating the progression of type 2 diabetes mellitus (T2DM). Here, Latilactobacillus sakei QC9 (L. QC9) with hypoglycemic effect was screened out from 30 food-derived strains through α-glucosidase and α-amylase activity inhibition tests in vitro 4-week vivo preliminary animal experiment. To further understand its alleviating on long-term hyperglycaemia occurring T2DM, we conducted an experiment that lasted 8 weeks. The results showed...
The reason why dietary polyunsaturated fatty acids (PUFAs) affect the activity of Lactobacillus remains unclear. In this study, linoleic acid was used to study mechanism underlying its inhibition function against activity.The growth curve rhamnosus LGG and metabolite content in bacterial liquid were determined at varying concentration. degree cell membrane damage L. by flow cytometry fluorescence microscopy, structure observed scanning electron microscopy transmission microscopy. effect on...
The Manchurian walnut (Juglans mandshurica Maxim.) is rich in proteins, whereas this resource has not been used efficiently.The antifatigue, antioxidative and immunoregulatory effects of hydrolysate peptides (MWHPs) were evaluated study.MWHPs with a degree hydrolysis 32.23% ultrafiltered divided into three fractions, namely, high (> 10 kDa), medium (3-10 low molecular weight (< 3 then fed to mice continuously at doses 200, 400 or 800 mg/(kg•d).The antioxidative, the tested on second fourth...
Hazelnut dregs are by-products of hazelnut oil expression, which have not been fully exploited. This research aims to assess the immunomodulatory function hydrolysed peptides (HHPs).HHPs with a hydrolysis degree 38.08% were divided into three fractions by ultra-filtration: high molecular weight peptide (>10 kDa), medium (3 kDa 10 and low (<3 kDa). Mice fed daily HHPs different weights at doses 200, 400, 800 mg kg(-1) body weight. On 10th, 20th 30th day feeding, representative immune indexes...
Akermansia muciniphila shows promise as a next-generation probiotic, however, its beneficial regulatory effects on mice ulcerative colitis (UC) has not been extensively investigated. We used an Akkermansia strain (AKK ONE) isolated from healthy human feces to study effect DSS-induced in mice. Our results demonstrate that AKK ONE supplementation significantly improves food intake, weight, colon length, disease activity index (DAI) score, organ index, and tissue damage of notably improved...
Objective . Staphylococcus aureus is an important pathogen that causes intestinal infection. We examined the immunomodulatory function of single and mixed Lactobacillus plantarum strains, as well their impacts on structure microbiome in mice infected with The experiment was divided into three groups: protection, treatment, control. Serum IFN- γ IL-4 levels, sIgA were measured during 1 week after infection without treatment. used 16s rRNA tagged sequencing to analyze composition. /IL-4 ratio...