Bacillus amyloliquefaciens is a nonpathogenic microorganism whose highly active amylase widely isolated from soil and plants. TL106 an isolate of cold- disease-resistant Tibetan pigs in Linzhi, Tibet. Here, we report that not only could survive acidic environments, high bile salt concentrations, high-temperature conditions but also was resistant to antibiotics. It significantly improved the growth performance weaned piglets, especially prevention diarrhea. The crude fiber ash digestibility...

Terminalia bellirica (Gaertn.) Roxb. (TB) is a traditional herbal combination used in Chinese medicine for the treatment of broad range diseases. In this study, thirty KM mice were randomly divided into control (N), infection group (NS), and TB protection (HS). Based on its digestive feature, intestinal physical barrier, immunological barrier gut microbiota effects vivo challenged with S.typhimurium investigated after oral administration 600 mg/kg b.wt 13 days. The results show that extract...

The occurrence of diarrhea in Tibetan piglets is highly notable, but the microorganisms responsible are yet unclear. Its high incidence results serious economic losses for pig industry. Moreover, dynamic balance intestinal microflora plays a crucial role maintaining host health, as it prime cause diarrhea. Therefore, present study was performed to analyze characteristics bacterial microbiota structure healthy, diarrheal and treated weaned Tibet autonomous region providing theoretical basis...

Abstract Background The nutrient availability of roughages could affect the dietary utilization efficiency ruminants even in isocaloric and isonitrogenous diets. Here, we analyzed bacterial composition their metabolic pathways gastrointestinal tracts (GITs) Hu sheep fed with wheat straw (WS) instead alfalfa (AL) diets, trying to explore reasons from perspective GITs network structure changes. Results We employed 16S rRNA gene sequencing combination Kruskal–Wallis test, Spearman correlation...

Gut microbiota are the microbial organisms that play a pivotal role in intestinal health and during disease conditions. Keeping view characteristic functions of gut microbiota, this study, Lactobacillus reuteri TPC32 (L. TPC32) was isolated identified, its whole genome analyzed by Illumina MiSeq sequencing platform. The results revealed L. had high resistance against acid bile salts with fine vitro antibacterial ability. Accordingly, sequence has total length 2,214,495 base pairs...

Xylanase has been demonstrated to improve growth performance of broilers fed wheat- or corn-based diets due its ability degrade arabinoxylans (AX). However, content and structure AX in corn wheat are different, comparing effects xylanase on cecal microbiota corn- wheat-based could further elaborate the mechanism specificity for different cereal grains. Thus, a total 192 one-day-old were randomly allotted into four dietary treatments, including wheat-soybean basal diet, diet with 4,000U/kg...

Abstract Background Diarrhea is an important ailment limiting the production of Tibetan pig industry. Dynamic balance intestinal microbiota for physiology animal. The objective this work was to study fungal diversity in feces early weaning piglets different health conditions. Results In present study, we performed high-throughput sequencing characterize microbial healthy, diarrheal and treated at Tibet Autonomous Region People’s Republic China. four alpha indices (Chao1, ACE, Shannon...

Zearalenone (ZEA) is widely present in feedstuffs and raw materials, causing reproductive disorders animals.In this study, Bacillus licheniformis CK1 was used to detoxify ZEA feed for alleviating its effect Tibetan piglets.A total of 18 weaned female piglets were randomly divided into 3 groups: control group (Control, ZEA-free basal diet); treatment 1 (T1, ZEA-contaminated 2 (T2, but pre-fermented by diet).There no significant differences average daily intake (ADFI), gain (ADG), efficiency...

Abstract Chickens reared in high altitude regions suffer from a mortality, possibly due to poor immune responses induced by hypoxia. This experiment was conducted evaluate whether increasing the oxygen level or administration of probiotic could improve mortality and response chickens at (2,986 m above sea level). One-d-old were randomly allocated 1 6 treatments 2 × 3 factorial arrangement. The first factor (low high), while second diet (basal diet, basal containing aureomycin, plus L....

Phytosterols are natural steroids in plants, possessing bioactivities that could modify gut microbes. This experiment aimed to evaluate the effects of feeding phytosterols on community structures and metabolic functions rumen microbiota perinatal cows. Perinatal cows were supplied with 0 mg (control) or 200 (treatment) per day. Multiomic analyses used analyze microbiota. Results showed dietary increased copy number total ruminal bacteria, concentration microbial crude protein, molar...

Abstract Background Cellulolytic microorganisms are considered a key player in the degradation of feed fiber. These can be isolated from various resources, such as animal gut, plant surfaces, soil and oceans. A new strain Bacillus amyloliquefaciens , TL106, was faeces healthy Tibetan pigs. This produce cellulase shows strong antimicrobial activity mice. Thus, this study, to better understand B. TL106 on cellulose, genome completely sequenced analyzed. In addition, we also explored cellulose...

A total of 240 1-day-old Arbor Acres male broilers were randomly divided into five dietary treatments (control feed (CON), supplemented with 75 mg/kg aureomycin (ANT), 7.5 × 108 CFU/kg (Ba1) and 2.5 109 (Ba1), (Ba3) Bacillus amyloliquefaciens TL106, respectively) to investigate the probiotic effect TL106 instead antibiotics in broilers. On days 1−21, average daily gain Ba groups was increased compared CON group (p < 0.05). In addition, feed/gain ratio lower than that ANT on 22−42 1−42...

This study aimed to investigate the changes in blood metabolic profiles of grazing yaks during cold season reveal their physiological status and seek nutrients needed be supplemented. Six castrated (3 years old) with 166.8 kg (standard deviation = 5.3) liveweight grazed Qinghai-Tibetan Plateau were used as experimental animals without supplementary feeding. Blood samples each animal collected October December 2015, March 2016 for analysis serum biochemicals metabolome. Results showed indices...

Currently, tylosin tartrate is the first-line treatment for Mycoplasma hyopneumoniae infections in China. However, efficacy of and resistance to this M. Tibetan pigs are unknown. In study, we examined prevalence infection at three intensive farms Tibet, addition, investigated porcine enzootic pneumonia by monitoring DNA eradication dynamics macrolide (MR). Eighty-two 450 (18.2%) tested positive only hyopneumoniae, most these animals (85.1%) had symptoms signs pneumonia. The elimination was...

Pseudorabies (PR) is an important emerging infectious disease that characterized by fever, extreme itching and encephalomyelitis. However, it still unclear whether Tibetan pigs are exposed to virus (PRV) or not. The present study was conducted investigate the seroprevalence of PRV infection in Nyingchi area Tibet through enzyme-linked immunosorbent assay (ELISA). A total 368 serum samples from were collected during 2015. Results showed 58 (15.76%) found positive for antibodies with further...

The soil microbiome is crucial in determining contemporary realistic conditions for future terrestrial ecological and evolutionary development. However, the precise mechanism between fecal deposition livestock grazing changes remains unknown. This first in-depth study of bacterial fungal taxonomic excrement contaminated soils plateau (&amp;gt;3,500 m). suggests functional shifts towards a harmful-dominated microbiome. According to our findings, contamination significantly reduced diversity...

Due to the high crude fiber content, straw of various crops is difficult become a quality forage resource. The degradation cellulose in nature mainly depends on cellulase secreted by microbes, which degrade into small molecular substances through chemical action, and microbes that secrete include some bacteria, fungi actinomycetes, etc. large diverse microbial population contained mammalian gastrointestinal tract plays an important role nutrient digestion. At present, many...

Objective: Previous research reported that dietary addition with phytosterols improved the energy utilisation of rumen microbiome, suggesting its potential to alleviate negative balance perinatal cows. This experiment aimed explore effects feeding on metabolic status cows through plasma metabolomics and faecal bacteria metabolism.Methods: Ten Holstein (multiparous, 2 parities) a similar calving date were selected four weeks before calving. After 7 days for adaptation, allocated two groups (n...

With the rapid development of animal husbandry, demand for protein feed resources is increasing. Cottonseed meal (CSM) and soybean (SBM) are rich sources protein. However, their application limited due to existence anti-nutrients, which can be harmful digestion absorption. A strain Bacillus subtilis (Mafic-Y7) was isolated from digesta intestines Tibetan pigs. The showed high protease activity, helps in degrading proteinic anti-nutritional factors grain vitro degradation free gossypol. In...

<title>Abstract</title> <bold>Background</bold> To enhance Niangya yak's reproductive efficiency and breeding process, this study employed Label-free quantitative proteomics technology to analyze fresh (F) chilled (C) semen samples.<bold>Result</bold> The analysis revealed an extensive repertoire of 18,338 peptide segments 2,579 proteins within yak semen. Among them, 2,300 were successfully annotated in the database. Notably, a total 300 DEPs identified between C F groups; comprising 129...