A5064742920- DNA Repair Mechanisms
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Cancer therapeutics and mechanisms
- Plant Genetic and Mutation Studies
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- T-cell and Retrovirus Studies
- Cancer-related Molecular Pathways
- PARP inhibition in cancer therapy
- RNA Interference and Gene Delivery
- Cell death mechanisms and regulation
- Pancreatic function and diabetes
- Aldose Reductase and Taurine
- RNA regulation and disease
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Carcinogens and Genotoxicity Assessment
- Cancer Immunotherapy and Biomarkers
- DNA and Nucleic Acid Chemistry
- Hepatitis B Virus Studies
- Cancer Mechanisms and Therapy
Hamamatsu University School of Medicine
2021-2024
Nagoya University
2020-2024
Hyogo Prefectural Amagasaki General Medical Center
2023
Ibaraki University
2004-2020
National Center for Global Health and Medicine
2010-2019
Kobe University
2012-2019
National Center For Child Health and Development
2016-2017
Retrograde menstruation is a widely accepted cause of endometriosis. However, not all women who experience retrograde develop endometriosis, and the mechanisms underlying these observations are yet understood. Here, we demonstrated pathogenic role Fusobacterium in formation ovarian In cohort women, 64% patients with endometriosis but <10% controls were found to have infiltration endometrium. Immunohistochemical biochemical analyses revealed that activated transforming growth factor–β...
DNA-protein cross-links (DPCs) are unique among DNA lesions in their unusually bulky nature. The steric hindrance imposed by cross-linked proteins (CLPs) will hamper transactions, such as replication and transcription, posing an enormous threat to cells. In bacteria, DPCs with small CLPs eliminated nucleotide excision repair (NER), whereas oversized processed exclusively RecBCD-dependent homologous recombination (HR). Here we have assessed the roles of NER HR for mammalian We show that upper...
Abstract Chemoresistance is a major cause of high mortality and poor survival in patients with ovarian cancer (OVCA). Understanding the mechanisms chemoresistance urgently required to develop effective therapeutic approaches OVCA. Here, we show that expression long noncoding RNA, taurine upregulated gene 1 (TUG1), markedly samples from OVCA who developed resistance primary platinum‐based therapy. Depletion TUG1 increased sensitivity cisplatin cell lines, SKOV3 KURAMOCHI. Combination therapy...
<p>Figure S1-S8 & Table S1-S6</p>
Abstract Overcoming drug resistance is one of the biggest challenges in cancer chemotherapy. In this study, we examine whether targeting long noncoding RNA taurine upregulated gene 1 (TUG1) could be an effective therapeutic approach to overcome pancreatic ductal adenocarcinoma (PDAC). TUG1 was expressed at significantly higher levels across 197 PDAC tissues compared with normal tissues. Overall survival patients who had undergone 5-FU–based chemotherapy shorter high group than low group....
Although pericentromeric heterochromatin is essential for chromosome segregation, its role in humans remains controversial. Dissecting the function of HIV-1–encoded Vpr, we unraveled important properties during segregation. In Vpr-expressing cells, hRad21, hSgo1, and hMis12, which are crucial proper were displaced from centromeres mitotic chromosomes, resulting premature chromatid separation (PCS). Interestingly, Vpr protein 1-α (HP1-α) HP1-γ chromatin. RNA interference (RNAi) experiments...
Long interspersed nucleotide element-1 (L1) is a retroelement comprising about 17% of the human genome, which 80–100 copies are competent as mobile elements (retrotransposition: L1-RTP). Although genetic structures modified during L1-RTP have been clarified, little known cellular signaling cascades involved. Herein we found that 6-formylindolo[3,2- b ]carbazole (FICZ), tryptophan photoproduct postulated candidate physiological ligand aryl hydrocarbon receptor (AhR), induces L1-RTP. Notably,...
Oncogene-induced DNA replication stress (RS) and consequent pathogenic R-loop formation are known to impede S phase progression. Nonetheless, cancer cells continuously proliferate under such high-stressed conditions through incompletely understood mechanisms. Here, we report taurine upregulated gene 1 (TUG1) long noncoding RNA (lncRNA), which is highly expressed in many types of cancers, as an important regulator intrinsic cells. Under RS conditions, TUG1 rapidly via activation the ATR-CHK1...
Viral protein R (Vpr) is an accessory of HIV-1, which potentially involved in the infection macrophages and induction ataxia-telangiectasia Rad3-related (ATR)-mediated DNA damage response (DDR). It was recently proposed that SLX4 complex structure-specific endonuclease Vpr-induced DDR, implies aberrant structures are responsible for this phenomenon. However, mechanism by Vpr alters remains unclear. We found unwinds double-stranded (dsDNA) invokes loading RPA70, a single-stranded DNA-binding...
Abstract Background Viral protein R (Vpr), a of human immunodeficiency virus type-1 (HIV-1) with various biological functions, was shown to be present in the blood HIV-1-positive patients. However, it remained unclear whether circulating Vpr patients’ is biologically active. Here, we examined activity using an assay system by which retrotransposition long interspersed element-1 (L1-RTP) detected. We also investigated vivo effects recombinant (rVpr) administrating transgenic mice harboring L1...
The criterion of a renal biopsy in children with asymptomatic persistent isolated proteinuria is controversial. To determine an adequate constant proteinuria, the optimal cutoff maximum urinary protein/creatinine ratio (uP/Cr) to separate minor glomerular abnormalities (MGA) and other significant changes was obtained by receiver operating characteristic analysis 44 (uP/Cr ≥0.2 g/g) screened from 1167 patients who underwent between September 2000 April 2010. Patients were divided into two...
Approximately 42% of the human genome is composed endogenous retroelements, and major retroelement component, long interspersed element-1 (L1), comprises ∼17% total genome. A single cell has more than 5 × 10(5) copies L1, 80∼100 which are competent for retrotransposition (RTP). Notably, L1 can induce RTP other such as Alu SVA, believed to function a driving force evolution. Although L1-RTP during early embryogenesis been highlighted in literature, recent observations revealed that also...
BRAF (V-raf murine sarcoma viral oncogene homolog B1) is a serine-threonine protein kinase involved in cell survival, proliferation, and differentiation. The most common missense mutation of (mainly V600E) contributes to the incidence various cancers, including Langerhans histiocytosis (LCH). inhibitors molecularly targeting V600E have been developed counteract effect mutation. To ensure administration effective pharmacotherapy, it therefore imperative develop an assay screen LCH patients...
Abstract The choice of repair pathways DNA double-strand breaks (DSBs) is dependent upon the cell cycle phases. While homologous recombination (HRR) active between S and G2 phases, its involvement in mitotic DSB has not been examined detail. In present study, we developed a new reporter assay system to detect homology-directed (HDR), major pathway used for HRR, combination with an inducible DSB-generation system. As expected, maximal HDR activity was observed late phase, along minimal G1...
ABSTRACT Viral protein R (Vpr) of HIV‐1 plays an important role in viral replication macrophages. Various lines evidence suggest that expression Vpr macrophages causes immunopathogenesis; however, the underlying mechanism is not yet fully understood. In this study, it was shown recombinant (rVpr) induces retrotransposition long interspersed element‐1 RAW264.7, a macrophage‐like cell line, and activates reverse transcriptase‐dependent immunotoxic cascades including production IFN‐β...
Improved genome-editing via oviductal nucleic acids delivery (i-GONAD) is a new technology that facilitates in situ of mammalian zygotes exiting the lumen. The i-GONAD has been developed for use mice, rats, and hamsters; however, oligonucleotide (ODN)-based knock-in (KI) more inefficient rats than mice. To improve efficiency we examined KI using three guide RNAs (gRNA), crRNA1, crRNA2 crRNA3. These gRNAs recognize different portions target locus, but also overlap each other locus. We effects...