A5051419857- Spaceflight effects on biology
- MicroRNA in disease regulation
- Electrical and Bioimpedance Tomography
- Bone Metabolism and Diseases
- Bone Tissue Engineering Materials
- Hemodynamic Monitoring and Therapy
- Bone health and osteoporosis research
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Anesthesia and Neurotoxicity Research
- Bone fractures and treatments
- Respiratory Support and Mechanisms
- High Altitude and Hypoxia
- Genetics, Aging, and Longevity in Model Organisms
- Body Composition Measurement Techniques
- Heart Rate Variability and Autonomic Control
- Complementary and Alternative Medicine Studies
- Histone Deacetylase Inhibitors Research
- GDF15 and Related Biomarkers
- Flow Measurement and Analysis
- Therapeutic Uses of Natural Elements
- EEG and Brain-Computer Interfaces
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Space Exploration and Technology
China Medical University
2025
First Hospital of China Medical University
2025
Sichuan Agricultural University
2025
Air Force Engineering University
2021-2024
Air Force Medical University
2015-2024
Ministry of Education of the People's Republic of China
2012-2013
Abstract MicroRNAs (miRNAs) interfere with the translation of specific target mRNAs and are thought to thereby regulate many cellular processes. However, role miRNAs in osteoblast mechanotransduction remains be defined. In this study, we investigated ability a miRNA respond different mechanical environments mechano-induced differentiation. First, demonstrated that miR-33-5p expressed by osteoblasts is sensitive multiple environments, microgravity fluid shear stress. We then confirmed promote...
Abstract Impaired osteoblast function is involved in osteoporosis, and microRNA (miRNA) dysregulation may cause abnormal osteogenic activity. However, the influence of miRNA on activity underlying mechanisms remain elusive. In this study, miR‐103‐3p was found to be negatively correlated with bone formation specimens from elderly women fractures ovariectomized (OVX) mice. Additionally, directly targeted Mettl14 inhibit activity, METTL14‐dependent N 6 ‐methyladenosine (m A) methylation...
Abstract Recent studies have demonstrated that miRNAs can play important roles in osteoblast differentiation and bone formation. However, the function of loss induced by microgravity remains unclear. In this study, we investigated differentially expressed both femur tissues hindlimb unloading rats primary rat osteoblasts (prOB) exposed to simulated microgravity. Specifically, miR-132-3p was found up-regulated negatively correlated with differentiation. Overexpression significantly inhibited...
Abstract Recent studies have confirmed that microRNAs and lncRNAs can affect bone cell differentiation formation. In this study, miR-139-3p was upregulated in the femurs of hindlimb unloading mice MC3T3-E1 cells under simulated microgravity; effect related to osteoblast apoptosis. Silencing attenuated suppression promotion apoptosis induced by microgravity. ELK1 is a target essential for regulate An differentiation-related lncRNA could interact with (lncRNA ODSM) identified Further...
Microglia play a crucial role in postoperative cognitive dysfunction (POCD). This study investigated the effects of microglial depletion and subsequent repopulation on POCD its underlying mechanisms. An aged mouse model was induced by partial hepatectomy, colony-stimulating factor 1 receptor (CSF1R) inhibitor PLX5622 administered to facilitate repopulation. Neutrophil involvement assessed with anti-Ly6G antibodies, while ZEB1 manipulated through shRNA knockdown lentiviral overexpression BV2...
L-type voltage-sensitive calcium channels (LTCCs), particularly Cav1.2 LTCCs, play fundamental roles in cellular responses to mechanical stimuli osteoblasts. Numerous studies have shown that loading promotes bone formation, whereas the removal of this stimulus under microgravity conditions results a reduction mass. However, whether exerts an influence on LTCCs osteoblasts and is possible mechanism underlying observed loss remain unclear. In present study, we demonstrated simulated...
Abstract Background Skeletal unloading can induce severe disuse osteopenia that often occurs in spaceflight astronauts or patients subjected to prolonged bed-rest immobility. Previously, we revealed a mechano-sensitive factor, miRNA-132-3p, is closely related the osteoblast function. The aim of this study was investigate whether miRNA-132-3p could be an effective target for treating osteopenia. Methods 2D-clinostat device and hindlimb-unloaded (HU) model were used copy mechanical condition...
Abstract Unloading-induced bone loss is a threat to human health and can eventually result in osteoporotic fractures. Although the underlying molecular mechanism of unloading-induced has been broadly elucidated, pathophysiological role long noncoding RNAs (lncRNAs) this process unknown. Here, we identified novel lncRNA, OGRU, 1816-nucleotide transcript with significantly decreased levels specimens from hindlimb-unloaded mice MC3T3-E1 cells under clinorotation-unloading conditions. OGRU...
Although electrical impedance tomography (EIT) is widely used for monitoring regional ventilation distribution, reference values have yet to be established clinical use. The present study aimed evaluate the feasibility of creating standard EIT parameters potential application.A total 75 participants with healthy lungs were included in this prospective (male:female, 48:27; age, 34±14 years; height, 172±7 cm; weight, 73±12 kg). subjects examined during spontaneous breathing supine position....
Abstract Objective. Wearable electrical impedance tomography (EIT) can be used to monitor regional lung ventilation and perfusion at the bedside. Due its special system architecture, amplitude of injected current is usually limited compared stationary EIT system. This study aims evaluate performance injection patterns with various low-amplitude currents in healthy volunteers. Approach. A total 96 test sets measurement was recorded 12 subjects by employing adjacent opposite four amplitudes...
Disuse osteoporosis is common in prolonged therapeutic bed rest, space flight and immobilization due to limb fracture, which related reduction of mechanical stress on bone. Mechanical unloading can inhibit the differentiation osteoblasts, but detailed mechanism still unclear. Runt-related transcription factor-2 (Runx2), an important factor, plays a crucial role disuse induced by conditions. In this study, we found that Runx2-targeting mechano-sensitive miR-30 family members, miR-30b,...
Background/aims: Microgravity leads to hydrodynamic alterations in the cardiovascular system and is associated with increased angiogenesis, an important aspect of endothelial cell behavior initiate new vessel growth. Given critical role Rho GTPase-dependent cytoskeleton rearrangement migration, small GTPase RhoA might play a potential microgravity-induced angiogenesis. Methods: We examined organization actin filaments by FITC-conjugated phalloidin staining, as well expression activity...
Abstract Calcium homeostasis in osteoblasts plays fundamental roles the physiology and pathology of bone tissue. Various types mechanical stimuli promote osteogenesis increase formation elicit increases intracellular‐free calcium concentration osteoblasts. However, whether microgravity, a condition unloading, exerts an influence on or what mechanisms may underlie such effect are unclear. Herein, we show that simulated microgravity reduces primary mouse In addition, substantially suppresses...
Electrical impedance tomography (EIT) is a bedside tool for lung ventilation and perfusion assessment. However, the ability long-term monitoring diminished due to interferences from clinical interventions motion artifacts. The purpose of this study investigate feasibility discrete wavelet transform (DWT) detect remove common types artifacts in thoracic EIT.Baseline drifting, step-like spike-like were simulated mimic three decomposition was employed characterize those different frequency...
We hypothesized that the respiratory exercises have uniform effects on ventilation in healthy subjects but varied patients with chronic obstructive pulmonary disease (COPD). In this study, a total of 30 volunteers and 9 COPD were included. Data recorded continuously during (1) diaphragmatic breathing; (2) pursed lip breathing full inhalation; (3) combining breathing. The sequence three was randomized using machine generated random permutation. Spatial temporal distributions evaluated...
Abstract A growing body of evidence has revealed that microRNAs (miRNAs) play crucial roles in regulating osteoblasts and bone metabolism. However, the effects miRNAs osteoblast mechanotransduction remain to be defined. In this study, we investigated regulatory effect miR-33-5p tested its anti-osteopenia when delivered by an osteoblast-targeting delivery system vivo. First, demonstrated could promote activity mineralization without influencing their proliferation vitro. Then our data showed...
Icariin (ICA), a major constituent of flavonoids from the Chinese medical herb Epimedium brevicornum Maxim, is found to be protective for male reproductive ability, with underlying mechanism largely unknown. Our study here investigated effects ICA on Sertoli cells, which act as nurse cells germ developing. was stimulate cell proliferation in dose-dependent manner. Further revealed that induced an obvious phosphorylation ERK cells. Inhibition activation by inhibitor U0126 nearly blocked...
The potential role of caveolin-1 in modulating angiogenesis microgravity environment is unexplored.Using simulated by clinostat, we measured the expressions and interactions eNOS human umbilical vein endothelial cells.We found that decreased expression associated with increased phosphorylation levels cells stimulated microgravity, which causes a dissociation from complexes. As result, induces cell migration tube formation vitro depends on regulations caveolin-1.Our study provides insight for...
Individualized lung-protective ventilation strategy is essential for patients with COVID-19. In this study, a wireless, low-power, and miniaturized electrical impedance tomography (EIT) system was developed remote long-term monitoring of lung COVID-19 in the isolation ward ICU. A new combining filtering improved voltage-controlled current employed to design source simplified structure, differential receiver circuit consisting buffer amplification proposed voltage measurement unit high...