A5037435382- Genetic Mapping and Diversity in Plants and Animals
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Cell Image Analysis Techniques
- Malaria Research and Control
- Computational Drug Discovery Methods
- Pancreatic function and diabetes
- Genetic and phenotypic traits in livestock
- Peroxisome Proliferator-Activated Receptors
- bioluminescence and chemiluminescence research
- Genetics, Aging, and Longevity in Model Organisms
- Metabolism, Diabetes, and Cancer
- Reproductive Biology and Fertility
- Mosquito-borne diseases and control
- Bat Biology and Ecology Studies
- Biochemical Analysis and Sensing Techniques
- Animal Genetics and Reproduction
- Mollusks and Parasites Studies
- Cutaneous Melanoma Detection and Management
- Diabetes and associated disorders
- Temporomandibular Joint Disorders
- Ovarian function and disorders
- Bone Metabolism and Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Bone health and osteoporosis research
Nagoya University
2014-2025
National Center for Geriatrics and Gerontology
2018
Chubu University
2010
The University of Texas Southwestern Medical Center
2010
Human Longevity (United States)
2001
Hamamatsu University School of Medicine
1998-2000
Red Cross Hospital
2000
Chubu National Hospital
1998-1999
Tokyo Dental College Ichikawa General Hospital
1991
Abstract The intricate link between glucose metabolism, ATP production, and glucose‐stimulated insulin secretion (GIIS) in pancreatic β‐cells has been well established. However, the effects of other digestible monosaccharides on this mechanism remain unclear. This study examined interaction intracellular fructose metabolism GIIS using MIN6‐K8 β‐cell lines mouse islets. Fructose at millimolar concentrations potentiated presence stimulatory levels (8.8 mM) glucose. potentiation was dependent...
The corneocyte lipid envelope, composed of covalently bound ceramides and fatty acids, is important to the integrity permeability barrier in stratum corneum, its absence a prime structural defect various skin diseases associated with defective function. SDR9C7 encodes short-chain dehydrogenase/reductase family 9C member 7 (SDR9C7) recently found mutated ichthyosis. In patient mutation mouse Sdr9c7-KO model, we show loss covalent binding epidermal protein, fault barrier. For reasons...
Heterozygous mutations in JAK1 which result JAK-STAT hyperactivity have been implicated an autosomal dominant disorder that features multi-organ immune dysregulation. This study identifies another previously unreported heterozygous missense mutation, H596D, individual with a unique autoinflammatory keratinization disease associated early-onset liver dysfunction and autism. Using CRISPR-Cas9 gene targeting, we generated mice identical Jak1 knock-in mutation ( H595D/+;I596I/+;Y597Y/+ mice)...
Objective The endocrine mechanisms linking fructose ingestion to glycemia remain unclear. This study revisited the of fructose-induced incretin and insulin secretion contextualize their roles in glycemic regulation following short-term administration. Methods Mice were administered 20% water ad libitum for 24 h. Plasma GLP-1 levels compared with those water-administered controls. mouse models included lean mice (C57BL/6J NSY.B6-a/a), obese diabetic (NSY.B6-Ay/a), proglucagon-deficient...
ABSTRACT Pathogenic variants in GFPT1, encoding a key enzyme to synthesize UDP-N-acetylglucosamine (UDP-GlcNAc), cause congenital myasthenic syndrome (CMS). We made knock-in (KI) mouse model carrying frameshift variant Gfpt1 exon 9, simulating that found patient with CMS. As 9 is exclusively expressed striated muscles, Gfpt1-KI mice were deficient for only skeletal muscles. In mice, (1) UDP-HexNAc, CMP-NeuAc and protein O-GlcNAcylation reduced muscles; (2) aged showed poor exercise...
Abstract Nevus-associated melanomas arise from pre-existing benign lesions, but de novo can also develop in the absence of such lesions. Few studies have addressed latter phenomenon because no animal models been described which clearly a manner. In this study, we address need defining RFP-RET-transgenic mice (RET mice) as mouse model for multi-step melanomagenesis that proceeds via tumor-free, benign, premalignant, and malignant stages. Melanomas RET exhibited decreased expression levels...
Changes of estrous cycles with aging F344/N rats between 1 and 30 months age (M) were monitored by vaginal smear cytology. The opening first cornified cell phase identified at 1.3 ± 0.1 M 1.5 0.2 M, respectively. Thereafter, showed about 5-day intervals, ceased 16.4 1.2 M. Thereafter irregular appearance single phases without the preceding nucleated interspersed a predominant leukocyte was seen in smears until 26.9 0.5 Growing mature follicles as well corpora lutea persisted least...
In the SMXA recombinant inbred (RI) strains, we measured body weight, blood insulin and lipid (triglyceride, total cholesterol phospholipid) levels in each strain. five traits, mean values of substrains varied remarkably showed a continuous spectrum distribution, suggesting control by multiple genes at distinct loci for trait. We also screened quantitative trait (QTLs) involved traits. Suggestive QTLs weight (Chromosomes 1 6), 1, 3, 10 17), triglyceride 4 11) phospholipid (Chromosome 18)...
Glutamine:fructose-6-phosphate transaminase 1 (GFPT1) is the rate-limiting enzyme of hexosamine biosynthetic pathway (HBP). A 54-bp exon 9 GFPT1 specifically included in skeletal and cardiac muscles to generate a long isoform (GFPT1-L). We showed that SRSF1 Rbfox1/2 cooperatively enhance, hnRNP H/F suppresses, inclusion human by modulating recruitment U1 snRNP. Knockout (KO) GFPT1-L muscle markedly increased amounts UDP-HexNAc, which subsequently suppressed glycolytic pathway. Aged KO mice...
A discrete deficiency of hepatic ornithine transcarbamylase (OTC) was found in male patients who were 58, 46 and 17 years old. Each had developed hyperammonemic coma. The mother a sister the 17-year-old patient exhibited orotic aciduria either spontaneously or after protein loading, thus demonstrating heterozygosity. one other daughter third showed smaller loading. These observations indicate that inherited OTC should be included differential diagnosis states adult patients.
To determine how to eliminate species difference in animal bone experiment, mineral content (BMC) was measured using dual energy X-ray absorptiometry (DXA) on the femurs of laboratory mice (Mus musculus) and rats (Rattus norvegicus), common marmosets (Callithrix jacchus). Measures were taken situ, detached from body, skinned defleshed, or dried completely. When BMC intact limb attached trunk set at 100%, actual dry 58.7 ± 11.5% 103.2 3.2% rats. Similarly, area (Area) density (BMD) femur...
Various mouse models of type 2 diabetes have been established, but few these show early onset and persistent hyperglycemia. We established a congenic strain (NSY.B6-Tyr+,Ay) in which spontaneous mutation the agouti yellow (Ay) gene, causes obesity by hyperphagia, was introduced into NSY strain, shows increased glucose intolerance with age. This has maintained as segregating inbred mating obese (Ay/a) males normal black (a/a) females. All showed marked hyperglycemia (mean blood level >400...
SMXA-5 mice are a high-fat diet-induced type 2 diabetes animal model established from non-diabetic SM/J and A/J mice. By using F2 intercross between under feeding with diet, we previously mapped major diabetogenic QTL (T2dm2sa) on chromosome 2. We then produced the congenic strain (SM.A-T2dm2sa (R0), 20.8–163.0 Mb) demonstrated that allele of T2dm2sa impaired glucose tolerance increased body weight mass index in compared to also showed combination other loci was needed develop diabetes. In...
The effects of PRL on fertilization and cleavage human oocytes subsequent pregnancy were studied. Forty-five patients (47 cycles) with euprolactinemic normal menstrual cycles undergoing in vitro (IVF) for the treatment tubal infertility selected this study. divided into three groups dependent upon their mean serum concentrations 3 days prior to Oocyte retrieval; hypoprolactinemic ( < 10 µg/I), (10–30 µg/l) hyperprolactinemic (≧30 µg/I). Multiple follicular development was induced hMG,...
Type 2 diabetes in humans is not a single gene disorder but multifactorial disease caused by the interaction of multiple genes and environmental factors. Recombinant inbred (RI) strains are powerful tool for analyzing only genetic traits also traits. By using SMXA RI mice, we genetically dissected diabetes-related (BMI, nonfasting blood glucose concentration, concentration during intraperitoneal tolerance tests). For minimizing variation each strain, all mice were fed high-carbohydrate diet...
We performed a genome-wide quantitative trait locus (QTL) analysis of body weight at 10 weeks age in population 321 intercross offspring from SM/J and A/J mice, progenitor strains SMXA recombinant inbred strains. Interval mapping revealed two significant QTLs, Bwq3 (body QTL3) Bwq4, on Chromosomes (Chrs) 8 18 respectively, five suggestive QTLs Chrs 2, 6, 7, 15 19. Bwq4 explained 6% the phenotypic variance. The alleles both increased weight, though mouse was smaller than mouse. On other hand,...
Quantitative trait locus (QTL) analysis of serum insulin, triglyceride, total cholesterol and phospholipid levels at 10 weeks age was performed in 321 F2 offspring from SM/J A/J mice. Interval mapping revealed a 22 suggestive QTLs affecting the four traits: two insulin on Chromosomes (Chrs) 6 8; six triglyceride Chrs 4, 8, 9, 11, 12 19; total-cholesterol 1, 3, 14, 17 eight 2, 6, 19. Gender influenced expression QTLs. The 14 17, QTL Chr 9 4 were specific to females. 2 8 males. In addition,...