A5050395223- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Cancer-related gene regulation
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Genetic Syndromes and Imprinting
- Down syndrome and intellectual disability research
- Pregnancy and preeclampsia studies
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Prenatal Screening and Diagnostics
- Histone Deacetylase Inhibitors Research
- Mathematical Biology Tumor Growth
- Machine Learning in Healthcare
- Cancer-related molecular mechanisms research
- Signaling Pathways in Disease
- Single-cell and spatial transcriptomics
- Renal and related cancers
- Pancreatic function and diabetes
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Peptidase Inhibition and Analysis
- Genomic variations and chromosomal abnormalities
City Of Hope National Medical Center
2019-2025
Center for Discovery
2018-2025
Hackensack Meridian Health
2018-2025
City of Hope
2019-2024
Beckman Research Institute
2019-2020
Hackensack University Medical Center
2019
Cancer Genetics (United States)
2004-2016
Columbia University Irving Medical Center
2004-2016
Columbia University
2006-2016
Center for Digestive and Liver Diseases
2012
Abstract Global hypomethylation has long been recognized as a feature of the malignant epithelial component in human carcinomas. Here we show evidence for this same type epigenetic alteration cancer-associated stromal myofibroblasts. We used methylation-sensitive SNP array analysis (MSNP) to profile DNA methylation early-passage cultures myofibroblasts isolated from gastric cancers. The MSNP data indicated widespread these cells, with rare focal gains methylation, conclusions that were...
The primary abnormality in Down syndrome (DS), trisomy 21, is well known; but how this chromosomal gain produces the complex DS phenotype, including immune system defects, not understood. We profiled DNA methylation total peripheral blood leukocytes (PBL) and T-lymphocytes from adults with normal controls found gene-specific abnormalities of CpG DS, many differentially methylated genes having known or predicted roles lymphocyte development function. Validation microarray data by bisulfite...
Trisomy 21 causes Down syndrome (DS), but the mechanisms by which extra chromosome leads to deficient intellectual and immune function are not well understood.Here, we profile CpG methylation in DS control cerebral cerebellar cortex of adults cerebrum fetuses. We purify neuronal non-neuronal nuclei T lymphocytes find biologically relevant genes with DS-specific (DS-DM) each these cell types. Some show brain-specific DS-DM, while others stronger DS-DM cells. Both 5-methyl-cytosine...
Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy that resists current treatments. To test epigenetic therapy against this cancer, we used the DNA demethylating drug 5-aza-2'-deoxycytidine (DAC) in an aggressive mouse model of stromal rich PDAC (KPC-Brca1 mice). In untreated tumors, found globally decreased 5-methyl-cytosine (5-mC) malignant epithelial cells and cancer-associated myofibroblasts (CAF), along with increased amounts 5-hydroxymethyl-cytosine (5-HmC) CAFs,...
From genomic association studies, quantitative trait loci analysis, and epigenomic mapping, it is evident that significant efforts are necessary to define genetic-epigenetic interactions understand their role in disease susceptibility progression. For this reason, an analysis of the effects genetic variation on gene expression DNA methylation human placentas at high resolution whole-genome coverage will have multiple mechanistic practical implications. By producing analyzing sequence (n =...
Abstract The product of the imprinted gene paternally expressed gene-10 (PEG10) has been reported to support proliferation in hepatocellular carcinomas, but how this is regulated an open question. We find that MYC knockdown by RNA interference suppresses PEG10 expression Panc1 pancreatic carcinoma and HepG2 cells inhibits Panc1, HepG2, Hep3B cells. Conversely, was up-regulated inducing c-MYC a B-lymphocyte cell line. Chromatin immunoprecipitation from showed bound E-box-containing region...
Abstract Background Down syndrome (DS) is caused by trisomy 21 (+21), but the aberrations in gene expression resulting from this chromosomal aneuploidy are not yet completely understood. Methods We used oligonucleotide microarrays to survey mRNA early- and late-passage control +21 fibroblasts mid-gestation fetal hearts. supplemented analysis with northern blotting, western real-time RT-PCR, immunohistochemistry. Results found chromosome genes consistently over-represented among...
Chromatin-associated RNAs (caRNAs) form a relatively poorly recognized layer of the epigenome. The caRNAs reported to date are transcribed from nuclear genome. Here, leveraging recently developed assay for detection and their genomic association, we report that mitochondrial (mtRNAs) attached genome constitute subset caRNA, thus termed mt-caRNA. In four human cell types analyzed, mt-caRNAs preferentially attach promoter regions. endothelial cells (ECs), level mt-caRNA-promoter attachment...
Mapping of allele-specific DNA methylation (ASM) can be a post-GWAS strategy for localizing regulatory sequence polymorphisms (rSNPs). The advantages this approach, and the mechanisms underlying ASM in normal neoplastic cells, remain to clarified.
Multiple Myeloma (MM) is a plasma cell malignancy arising in the bone marrow. Despite advancements therapies, this disease becomes refractory such that relapse and death inevitable. Immune evasion drug resistance are partly due to MM-osteoblast interactions marrow microenvironment (BME). We conducted complementary in-vitro in-vivo studies, each providing insights converge treatment-relevant hypothesis. Several human MM lines (MM.1S, U266, NCI-H929, L363) were co-cultured with osteoblasts for...
Starting at middle age, adults often suffer from visceral adiposity and associated adverse metabolic disorders. Lineage tracing in mice revealed that adipose progenitor cells (APCs) fat undergo extensive adipogenesis during age. Thus, despite the low turnover rate of adipocytes young adults, is unlocked Transplantations quantitatively showed APCs middle-aged exhibited high adipogenic capacity cell-autonomously. Single-cell RNA sequencing identified a distinct APC population, committed...
Chronic psychological stress is a prominent risk factor involved in the pathogenesis of many complex diseases, including major depression, obesity, and type II diabetes. Visceral adipose tissue key endocrine organ regulation insulin action an important component development resistance. Here, we examined for first time changes on visceral physiology adipocyte-associated sensitivity function after chronic unpredictable rats. Male rats were subjected to 35 days. Total body fat was measured....
CD4+ T cell interactions with B cells play a critical role in the pathogenesis of systemic autoimmune diseases such as lupus and chronic graft-versus-host disease (cGVHD). Extrafollicular CD44hiCD62LloPSGL1loCD4+ (PSGL1loCD4+ cells) are associated cGVHD, but their causal has not been established. With murine humanized MHC-/-HLA-A2+DR4+ models we showed that human PSGL1loCD4+ from GVHD target tissues have features helpers upregulated expression programmed death protein 1 (PD1) inducible...
Abstract The phenotype of human placental extravillous trophoblast (EVT) at the end pregnancy reflects both first trimester differentiation from villous cytotrophoblast (CTB) and later gestational changes, including loss proliferative invasive capacity. Invasion abnormalities are central to two major pathologies, preeclampsia placenta accreta spectrum, so characterization corresponding normal processes is crucial. In this report, our gene expression analysis, using purified CTB EVT cells,...
To determine whether DNA methylation patterns in genes coding for selected T-lymphocyte proteins are associated with perinatal psychiatric distress or complications of pregnancy.T lymphocyte was obtained from pregnant women across three time points pregnancy and the postpartum period epigenetic were assessed using Illumina 450 K Methylation Beadchips. Seven critical T cell function analyzed changes during associations complications, particular attention paid to spatial aggregations methyl...
You have accessJournal of UrologyKidney Cancer: Basic Research & Pathophysiology II (PD51)1 May 2024PD51-05 ALLELE-SPECIFIC DNA METHYLATION AFFECTING THE STEAP3 GENE IN RENAL CELL CARCINOMAS Mauricio Menagati Rigo, Angelica Castano, Martha C. Salas, Mariam Tariq, Joshua Lerman, Sophia Hartley, Fidi Nagy, Peter L. Michael Raver, Kar Chow, Nitin Yerram, D. Stifelman, and Benjamin Tycko RigoMauricio Rigo , CastanoAngelica Castano SalasMartha Salas TariqMariam Tariq LermanJoshua Lerman...