A5001083935- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Glioma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Galectins and Cancer Biology
- Sarcoma Diagnosis and Treatment
- Vitamin D Research Studies
- Legionella and Acanthamoeba research
- Head and Neck Surgical Oncology
- Diabetes and associated disorders
- Cytokine Signaling Pathways and Interactions
- Head and Neck Cancer Studies
- Cancer, Hypoxia, and Metabolism
- Antibiotic Resistance in Bacteria
- Neurofibromatosis and Schwannoma Cases
- COVID-19 and healthcare impacts
- Vitamin C and Antioxidants Research
- Protein purification and stability
- Bacterial Infections and Vaccines
- Immunodeficiency and Autoimmune Disorders
- Antibiotics Pharmacokinetics and Efficacy
- Neuroendocrine Tumor Research Advances
University of California, San Francisco
2022-2025
Oklahoma Medical Research Foundation
2011-2024
Xiangya Hospital Central South University
2024
Central South University
2024
University of California San Francisco Medical Center
2024
University of Oklahoma Health Sciences Center
2012-2021
University of Oklahoma Medical Center
2018
OU Health
2016
University of Oklahoma
1989-2015
First People's Hospital of Foshan
2014
The relationship of immune dysregulation and autoantibody production that may contribute to systemic lupus erythematosus (SLE) pathogenesis is unknown. This study evaluates the individual combined contributions autoantibodies, type I interferon (IFN-α) activity, IFN-associated soluble mediators disease development leading SLE.Serial serum specimens from 55 individuals collected prior SLE classification (average timespan=4.3 years) unaffected healthy controls matched by age (±5 years),...
Objective Antinuclear antibodies (ANAs) are detected in ∼18% of females, yet autoimmune disease develops only 5–8%. Immunologic differences between ANA‐positive healthy individuals and patients with systemic lupus erythematosus (SLE) may elucidate the regulatory mechanisms by which avoid transition to clinical disease. Methods Healthy (n = 790) were screened for autoantibodies specific 11 antigens associated lupus, sclerosis, Sjögren's syndrome. From this screening, 31 European American...
A patient with well-controlled type 2 diabetes mellitus developed a severe pulmonary infection secondary to Rhizopus spp. after receiving short courses of corticosteroids for respiratory tract infection. He recovered an aggressive surgical intervention and treatment isavuconazole. Patients on chronic corticosteroid therapy have higher risk mucormycosis, but there are much fewer reports mucormycosis occurring in patients only steroid therapy.
The clinical and pathologic diversity of systemic lupus erythematosus (SLE) hinders diagnosis, management, treatment development. This study addresses heterogeneity in SLE through comprehensive molecular phenotyping machine learning clustering. Adult patients (n = 198) provided plasma, serum, RNA. Disease activity was scored by modified SELENA-SLEDAI. Twenty-nine co-expression module scores were calculated from microarray gene-expression data. Plasma soluble mediators 23) autoantibodies 13)...
A patient in California, USA, with rare and usually fatal Balamuthia mandrillaris granulomatous amebic encephalitis survived after receiving treatment a regimen that included the repurposed drug nitroxoline. Nitroxoline, which is quinolone typically used to treat urinary tract infections, was identified screen for drugs amebicidal activity against Balamuthia.
Abstract Objective Replacement of standard immunofluorescence methods with bead‐based assays for antinuclear antibody (ANA) testing is a new clinical option. The aim this study was to evaluate large, multiethnic cohort patients systemic lupus erytematosus (SLE), blood relatives, and unaffected control individuals familial aggregation subset clustering autoantibodies by high‐throughput serum screening technology traditional methods. Methods Serum samples (1,540 SLE patients, 1,154 906...
Abstract Gliomas arising in the setting of neurofibromatosis type 1 (NF1) are heterogeneous, occurring from childhood through adulthood, can be histologically low-grade or high-grade, and follow an indolent aggressive clinical course. Comprehensive profiling genetic alterations beyond NF1 inactivation epigenetic classification these tumors remain limited. Through next-generation sequencing, copy number analysis, DNA methylation gliomas 47 patients, we identified 2 molecular subgroups...
Altered signalling in B cells is a predominant feature of systemic lupus erythematosus (SLE). The genes BANK1 and BLK were recently described as associated with SLE. codes for B-cell-specific cytoplasmic protein involved B-cell receptor an Src tyrosine kinase important roles development. To characterise the role SLE, genetic interaction analysis was performed hypothesising that interactions could reveal functional pathways relevant to disease pathogenesis.The GPAT16 method used analyse...
Abstract Objective To address heterogeneity complicating primary SS (pSS) clinical trials, research and care by characterizing clustering patients their molecular phenotypes. Methods pSS met American–European Consensus Group classification criteria had at least one systemic manifestation stimulated salivary flow of ⩾0.1 ml/min. Correlated transcriptional modules were derived from gene expression microarray data blood (n = 47 with appropriate samples). Patients clustered based on this...
We examined whether measures of vitamin D were associated with transitioning to systemic lupus erythematosus (SLE) in individuals at risk for SLE.436 who reported having a relative SLE but did not have themselves evaluated baseline and again an average 6.3 (±3.9) years later. Fifty-six transitioned (≥4 cumulative American College Rheumatology criteria). 25-Hydroxyvitamin (25[OH]D) levels measured by ELISA. Six single-nucleotide polymorphisms four genes genotyped. Generalised estimating...
Systemic lupus erythematosus (SLE) is a highly variable autoimmune disease that can involve severe organ-threatening symptoms, such as nephritis. Certain drugs, mycophenolate mofetil (MMF), are effective at reducing morbidity associated with nephritis; however, the immune pathways suppression poorly defined. Here, we provide evidence MMF inhibits phosphorylation of STAT3 and other pathways. Using mass cytometry bead-based or ELISA assays, systemic phenotype SLE patients not taking (MMF-)...
Low-grade gliomas and reactive piloid gliosis can present with overlapping features on conventional histology. Given the large implications for patient treatment, there is a need effective methods to discriminate these morphologically similar but clinically distinct entities. Using routinely available stains, we hypothesize that limited panel including SOX10, p16, cyclin D1 may be useful in differentiating mitogen-activated protein (MAP) kinase-activated low-grade from gliosis. Reviewers...
Objective In recent years, vitamin D has been shown to possess a wide range of immunomodulatory effects. Although there is extensive amount research on D, we lack comprehensive understanding the prevalence deficiency or mechanism by which regulates human immune system. This study examined and correlates relationship between system in healthy individuals. Methods Healthy individuals (n = 774) comprised European-Americans (EA, n 470), African–Americans (AA, 125), Native Americans (NA, 179)...
SLE is a clinically heterogeneous disease characterized by an unpredictable relapsing-remitting course. Although the etiology and mechanisms of flares remain elusive, Epstein-Barr virus (EBV) reactivation implicated in pathogenesis. This study examined relationships between serological measures EBV reactivation, activity, interferon (IFN)-associated immune pathways patients. Sera from adult patients (n = 175) matched unaffected controls 47) were collected tested for antibodies against...
Abstract The location of the active site membrane-bound anticoagulant complex thrombin and thrombomodulin has been determined relative to membrane surface using fluorescence energy transfer. Thrombin was reacted with 5-(dimethylamino)-1-naphthalenesulfonylglutamylglycylarginyl chloromethyl ketone (DEGR-CK) yield DEGR-thrombin, an analogue a fluorescent dye covalently attached its site. When DEGR-thrombin titrated that had reconstituted into phospholipid vesicles containing...
Purpose . This study evaluates high-throughput autoantibody screening and determines associated systemic lupus erythematosus (SLE) clinical features in a large cohort. Methods Clinical demographic information, along with serum samples, were obtained from each SLE participant after appropriate informed consent. Serum samples screened for 10 distinct specificities examined association ACR criteria subcriteria using conditional logistic regression analysis. Results In European-American...
Abstract Systemic lupus erythematosus (SLE) flares elicit progressive organ damage, leading to disability and early mortality. This study evaluated clinical immunologic factors associated with impending flare in the Biomarkers of Lupus Disease study. Autoantibodies 32 soluble mediators were measured by multiplex assays, immune pathway activation gene expression module scores, cell subset frequencies states flow cytometry. After providing baseline samples, participants received transient...
The clot-lysing ability of streptokinase (SK) was examined using membrane-bound thrombi. Encapsulation SK in large unilamellar phospholipid vesicles (liposomes) resulted entrapping approximately 30% its original activity. Measurements activity for liposomal-encapsulated (LESK) indicated little loss or leakage Tris-buffered saline over a 24-hr period at temperatures 4 and 23 degrees C. However, incubation free LESK platelet-poor plasma (PPP) 37 C decrease retention considerably higher than...
Composite pleomorphic xanthoastrocytoma-ganglioglioma (PXA-GG) is an extremely rare central nervous system neoplasm with 2 distinct but intermingled components. Whether this tumor represents a "collision tumor" of separate neoplasms or monoclonal divergent evolution poorly understood. Clinicopathologic studies and capture-based next generation sequencing were performed on extracted DNA from all available PXA-GG at medical centers. Five diagnosed in 1 male 4 female patients ranging 13 to 25...