A5036374660- Immune cells in cancer
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- Neonatal Respiratory Health Research
- Influenza Virus Research Studies
- Extracellular vesicles in disease
- Tryptophan and brain disorders
- COVID-19 Impact on Reproduction
- Intensive Care Unit Cognitive Disorders
- Respiratory Support and Mechanisms
- Mast cells and histamine
- Autophagy in Disease and Therapy
- Long-Term Effects of COVID-19
- Congenital Diaphragmatic Hernia Studies
- Immune Response and Inflammation
- Tissue Engineering and Regenerative Medicine
- COVID-19 Clinical Research Studies
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Vaccine Coverage and Hesitancy
- melanin and skin pigmentation
- Immune responses and vaccinations
- MicroRNA in disease regulation
University of Virginia
2022-2024
Pudong New Area People's Hospital
2023
Carter Center
2022-2023
Mayo Clinic
2020-2023
Mayo Clinic in Arizona
2020-2022
Mayo Clinic in Florida
2020-2022
WinnMed
2019-2022
Abstract Despite intensive characterization of immune responses after COVID-19 infection and vaccination, research examining protective correlates vertical transmission in pregnancy are limited. Herein, we profiled humoral cellular characteristics pregnant women infected or vaccinated at different trimesters their corresponding newborns. We noted a significant correlation between spike S1–specific IgG antibody its RBD-ACE2 blocking activity (receptor-binding domain–human...
Abstract Post-acute sequelae of SARS-CoV-2 infection (PASC) represents an urgent public health challenge, with its impact resonating in over 60 million individuals globally. While a growing body evidence suggests that dysregulated immune reactions may be linked PASC symptoms, most investigations have primarily centered around blood studies, few focusing on samples derived from post-COVID affected tissues. Further, clinical studies alone often provide correlative insights rather than causal...
Acute lung injury (ALI) is a life-threatening disease that currently lacks cure. Although stem cell-derived small extracellular vesicles (sEVs) have shown promising effects in the treatment of ALI, their underlying mechanisms and responsible components yet to be identified. Proprotein convertase subtilisin/kexin type 6 (PCSK6) gene involved inflammation potential target miR-21-5p, microRNA enriched sEVs. The current study investigated role PCSK6 lipopolysaccharide (LPS)-induced ALI its...
Abstract Lower respiratory viral infections, such as influenza virus and severe acute syndrome coronavirus 2 (SARS-CoV2) often cause pneumonia in aged individuals. Here, we report that leads to chronic non-resolving lung pathology exaggerated accumulation of CD8 + tissue-resident memory T cells (T RM ) the tract hosts. relies on elevated TGF-β present tissues. Further, show isolated from lungs lack a subpopulation characterized by expression molecules involved TCR signaling effector...
Abstract Respiratory viral infections such as influenza and SARS-CoV-2 can lead to the development of chronic lung disease impaired function long-term after recovery from acute morbidity. However, mechanisms underlying diseases post-acute respiratory infection are largely elusive. Using a model age-related infection, we found that population resident PD1HiCD4+ T helper cells (TRH), which exhibit characteristics both follicular memory cells, was greatly increased primary pneumonia....
Abstract The role of Bcl6 expression in different immune compartments regulating anti-tumor immunity is relatively unknown. Previous studies have linked PBMC with poor prognosis non-small cell lung cancer. We found that high tumor predicts survival gastric and ovarian cancers. Using type specific conditional knockouts, we were able to investigate the contribution lineages modulating response subcutaneously implanted B16-ova melanoma tumors. Myeloid deficiency results decreased growth...
Abstract CD8+ tissue-resident memory T (TRM) cells provide frontline immunity in mucosal tissues. The mechanisms regulating TRM maintenance, heterogeneity, and protective pathological functions are largely elusive. Here, we identify a population of that is maintained by major histocompatibility complex class I (MHC-I) signaling, CD80 CD86 co-stimulation after acute influenza infection. These have both “exhausted-like” phenotypes features, heterologous against secondary Using recombinant...
Abstract Alveolar Macrophages (AMs) are unique innate immune cells that reside in the alveolar space and accommodate ever-changing need of lungs against internal external challenges. During homeostasis, AMs maintained themselves through self-renewal without for input from adult hematopoietic stem cells. Currently, little is known about how aging influences AM dynamics, heterogeneity self-renewal. To this end, we have performed single-cell RNA sequencing (scRNA-seq) on murine lung (CD11+...
Alveolar macrophages (AMs) are the most abundant immune cells in lung during homeostasis. AMs critical for normal development, surfactant metabolism and host defense against various respiratory pathogens. Despite being terminally differentiated cells, able to proliferate self-renew maintain their compartment without input of hematopoietic system adulthood However, molecular metabolic mechanisms modulating AM proliferative responses still incompletely understood. Here we have investigated...
Abstract Sialic acids are the terminal modification on glycoproteins and glycolipids. The sialic acid transferase ST8Sia6 generates a2,8-linked disialic O-linked glycoproteins, which bind to inhibitory receptor Siglec-E innate immune cells suppress activation. Recently, our lab has shown that expression tumor inhibits response through Siglec-E, leading increased growth decreased survival. Therefore, products of can modulate tumors. Our generated knockout (KO) mice, we seek understand role in...
Abstract Alveolar Macrophages (AMs) are a unique population of innate immune cells that reside in the alveolar space and contribute to accommodate ever-changing need lungs against external environmental challenges. AMs derive from fetal origins capable maintaining themselves through self-renewal without input hematopoiesis during adulthood. However, little is known about how aging influencing molecular characteristics heterogeneity homeostasis. Using single-cell RNA sequencing (scRNA-seq)...
Abstract Fetal-derived tissue-resident macrophages exhibit stem cell-like features of self-renewal in adulthood to maintain macrophage population during homeostasis and/or various insults. However, little is known about the cellular and molecular mechanisms modulating proliferative inflammatory fate decisions vivo. Here, we show that WNT-β-catenin signaling inhibited lung-resident alveolar (AM) self-renewal, while simultaneously promoted AM activities vitro vivo influenza virus infection....
Abstract Infants born prematurely often develop chronic lung diseases including bronchopulmonary dysplasia (BPD). BPD is associated with greatly increased host susceptibility and severe disease development following respiratory viral infection. To this end, we have recently established a neonatal hyperoxia model infection in hosts prematurity BPD. Using model, observed that mice exposed (80% oxygen, hosts) developed enhanced influenza virus or syncytial (RSV) compared to those of...
Abstract Severe influenza A virus infection typically triggers excessive and detrimental lung inflammation with massive cell infiltration hyper-production of cytokines chemokines. Defining research models to alleviate damage without compromising viral control is critical. We identified a novel function for nuclear matrix protein 4 (NMP4), zinc-finger-containing transcription factor in regulating antiviral innate immune responses. Nmp4-deficient mice are protected from H1N1 infection, losing...
Abstract Background Despite extensive studies of human immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and disease 2019 (COVID-19) vaccination, research examining protective correlates vertical transmission following maternal exposure in pregnancy remain limited. Here, we characterized antibody cytokine cord blood or vaccination at various timepoints during gestation. Methods Spike S1 protein-specific binding antibodies capable blocking the...
Abstract The humoral immune response requires that B cells undergo a rapid metabolic shift and high demand of nutrients, which are vital to sustain the formation germinal center. Rag GTPase senses amino acid availability activate mechanistic target rapamycin complex 1(mTORC1) pathway modulate function transcription factor EB (TFEB), member microphthalmia (MiT/TFE) family HLH-leucine zipper factors. However, little is known about how coordinates sensing, mTORC1 activation TFEB activity in...
Abstract Global studies have established age as the most critical risk factor in determining morbidity and mortality following respiratory viral infections such influenza SARS-CoV2. Previous from our group identified non-resolving inflammation aged lungs post infection, primarily driven by persistence of tissue resident memory (Trm) CD8 T-cells. We also observed sustained impaired lung function COVID19 convalescents, which negatively correlated with levels pulmonary Trm cells. While for...
Abstract Individuals with hyperglycemia are at great risk of developing exaggerated pulmonary inflammation following infection respiratory viruses including SARS-CoV-2. Conversely, SARS-CoV-2 has been linked to the development new onset and diabetes. Despite global vaccination efforts, continues be a public health challenge, corticosteroids targeting hyper-inflammation remain mainstay treatment for severe COVID-19. However, steroidal medicine arises hyperglycemia, limiting its usage. Here,...
Abstract Aging has been shown to be one of the major risk factors for host morbidity and mortality following infections with respiratory viruses including influenza virus SARS-CoV-2. The unique immune properties aged hosts not only influence pathogen clearance tissue damage responses, but also modulate pathogenesis long-term sequalae post acute viral clearance. Currently, dynamics features mucosal responses in remain fully elucidated. Here, we characterized lung cellular molecular profiles...
Abstract Alveolar Macrophages (AMs) are unique innate immune cells that reside in the alveolar space and accommodate ever-changing need of lungs against internal external challenges. During homeostasis, AMs maintained themselves through self-renewal without for input adult hematopoietic stem cells. Currently, little is known about how aging influences AM dynamics, heterogeneity transcriptional profiles. Here, we identified transcription factor, Cbfb, has indispensable role self-renew...