Epithelial-mesenchymal transition (EMT) is a physiological process that has been recognized to occur during the progression of an increasingly large number human diseases, including liver fibrosis, cirrhosis, and hepatocellular carcinoma. The activation transforming growth factor β (TGF-β) signaling considered critical event EMT, efforts have made screen small molecules interfere with TGF-β pathway EMT. Here we report identification sorafenib, clinical agent inhibits signaling. When applied...

Background Cotton (Gossypium spp.) is a model system for the analysis of polyploidization. Although ascertaining donor species allotetraploid cotton has been intensively studied, sequence comparison Gossypium chloroplast genomes still interest to understand mechanisms underlining evolution allotetraploids, while it generally accepted that parents were A- and D-genome containing species. Here we performed comparative 13 genomes, twelve which are presented here first time....

The cotton genus (Gossypium spp.) contains 8 monophyletic diploid genome groups (A, B, C, D, E, F, G, K) and a single allotetraploid clade (AD). To gain insight into the phylogeny of Gossypium molecular evolution chloroplast in this group, we performed comparative analysis 19 genomes, six reported here for first time. Nucleotide distance non-coding regions was about three times that coding regions. As expected, distances were smaller within than among groups. Phylogenetic topologies based on...

Agave hybrid cultivar 11,648 has been planted for sisal fiber production in China since the 1960s. However, little is known about population structure and genetic diversity of agave germplasms China. Therefore, we developed a group core SNP markers to evaluate 125 China, including 20 cultivars, 14 breeding lines, 89 transplanted resources from different areas. Cost-effective amplicon sequencing technology was used identify variants. The results grouped most cultivars lines together, which...

Objective: To explore the mediating effects of perceived social support between frailty and self-perceived burden (SPB) in elderly patients with diabetes to provide a theoretical basis for reducing that burden.Methods: A total 169 who were hospitalised endocrinology department third-class hospital Wuxi May 2020 July 2022 included this study using convenience sampling method.Patients assessed by general information questionnaire, Chinese version Tilburg inventory (TFI), Self-Perceived Burden...

P4-ATPases are transmembrane proteins unique to eukaryotes that play a fundamental role in vesicular transport. They have been proposed act as phospholipid flippases thereby regulating lipid topology cellular membranes. We cloned and characterized novel murine P4-ATPase is specifically expressed testis, named it FetA (flippase testis splicing form A). When Saccharomyces cerevisiae, localizes partially the plasma membrane resulting increased internalization of NBD-labeled...

P 4 ‐ATPases are lipid flippases that transport phospholipids across cellular membranes, playing vital roles in cell function. In humans, the disruption of ‐ATPase ATP8A2 gene causes a severe neurological phenotype. Here, we found Atp8a2 mRNA was highly expressed PC12 cells, hippocampal neurons and brain. Overexpression increased length neurite outgrowth NGF‐induced cells primary cultures rat neurons. Inducing loss function CDC50A via RNA interference reduced outgrowth, co‐overexpression...

Efficient and modular genome editing technologies that manipulate the of bacterial pathogens will facilitate study pathogenesis mechanisms. However, such methods are yet to be established for Xanthomonas oryzae pv. (Xoo), causal agent rice blight. We identified a single type I-C CRISPR-Cas system in Xoo leveraged this endogenous defence high-efficiency Xoo. Specifically, we developed plasmid components carrying mini-CRISPR array, donor DNA, phage-derived recombination enable efficient...

Agave species are widely planted for fiber production. However, the molecular basis of agave development has not been well understood. In this study, we performed a transcriptomic analysis in A. amaniensi, well-known variety with high-quality Approximately 43.87 million clean reads were obtained using Illumina sequencing. The de novo assembly produced 66,746 unigrams, 54% which annotated public database. Nr database, 21,490 unigenes amaniensis shown to be most closely related Asparagus...

Introduction The angiotensin-converting enzyme-2 (ACE-2) and its shedding product [soluble ACE-2 (sACE-2)] are implicated in adverse cardiovascular outcomes. However, the relationship between sACE-2 stroke recurrence is unknown. Herein, we examined of with patients ischemic or transient attack (TIA). Methods Data were obtained from Third China National Stroke Registry (CNSR-Ⅲ). Eligible cases consisted 494 who developed recurrent within 1-year follow-up, controls selected using age- sex-...

This study aimed to explore the effects of different types xanthophyll extracted from marigold on growth performance, skin color, and carcass pigmentation.A total 192 healthy 60-day-old yellow-feathered broilers weighing an average 1,279±81 g were randomly allocated 4 groups, each with 6 replicates 8 broilers. The treatments as follows: i) CON group, fed basal diet; ii) LTN supplemented lutein; iii) MDP monohydroxyl pigment including dehydrated lutein, β-cryptoxanthin, α-cryptoxanthin; iv)...

Agave amaniensis Trel. & W. Nowell (1933) has long been used for phytosteroid production, which is also one of the parents famous hybrid cultivar 11648 sisal fiber production. However, its systematic position and phylogenetic relationship remains unknown at chloroplast (cp) genome level. Therefore, we have sequenced assembled cp A. via Illumina sequencing. The 157,282 bp in length with a GC content 37.84%. A large single-copy region 85,899 bp, small 18,233 inverted repeat regions 26,575 were...

Agave angustifolia is an important crassulacean acid metabolism plant species, with wide applications in beverage and sisal fiber production. In this study, we carried out a transcriptome analysis of A. leaves, generating total 58,482,436 clean reads through Illumina paired-end sequencing. De novo assembly generated 67,314 unigenes, about half them having homologs four public databases. the Nr database, Asparagus officinalis was shown to be most closely related agave, early angiosperm...

Agave hybrid 11648 is the most widely cultivated agave variety for sisal fiber production around world. In present study, we have successfully sequenced chloroplast genome of A. H11648. The complete size 157,274 bp in length with a GC content 37.8%. contains large single copy region (LSC) 85,896 bp, small (SSC) 18,230 and pair inverted repeat regions (IR) 26,574 bp. 121 genes are annotated genome. numbers protein-coding, tRNA rRNA 99, 40 8, respectively. Phylogenetic tree reveals that H11648...

BACKGROUND Perihilar cholangiocarcinoma (pCCA) is a highly malignant tumor arising from the biliary tree. Radical surgery only treatment offering chance of long-term survival. However, limited by tumor’s anatomic location and peri-vascular invasion, most patients lose for curative treatment. Therefore, more methods to increase resectability tumors as well improve outcomes are needed. CASE SUMMARY A 68-year-old female patient had hepatic hilar mass without obvious symptoms. Laboratory results...

Agave fourcroydes (henequen) is the only cultivated species in Yucatan Peninsula, which mainly used for fiber production. In present study, we have successfully assembled chloroplast (cp) genome of A. fourcroydes. The full length cp 157,291 bp with a GC content at 37.8%. constructed an inverted repeat region (IRa) 26,573 bp, small single copy (SSC) 18,230 b (IRb) and large (LSC) 85,915 bp. annotation result reveals 132 genes on genome, including 86 protein-coding genes, 38 tRNAs 8 rRNAs....

Agave angustifolia is commonly used for the production of bacanora, a kind fermented and distilled beverage in Mexico. In present study, we have successfully assembled its chloroplast genome. The full length genome 157,274 bp with GC content 37.84%. There large single copy region (LSC) 85,895 bp, pair inverted repeat regions (IR) 26,575 small (SSC) 18,229 A total 132 genes are annotated cp Among these, there 86 protein-coding genes, 38 tRNAs 8 rRNAs. Phylogenetic analysis reveals that A....

<title>Abstract</title> Lung cancer is the most common oncological disease worldwide, with non-small cell lung accounting for approximately 85% of cases. α-Hederin a monodesmosidic triterpenoid saponin isolated from leaves Hedera helix L. or Nigella sativa and has been extensively studied its antitumor activity against variety tumor cells. It suggested that potential regulator autophagy high promise application. However, specific mechanism characteristics in regulating are not well...

In order to study the effect of food and kitchen waste on promoting methane production by collaborative fermentation coal, this paper selected lignite as research objects, carried out gas experiment mixed coal with different ratios at 35 ℃ mine water bacteria source. The results showed that cumulative CH4 was highest in ratio 1:4 (coal: waste). Therefore, exploring optimal mixture can not only effectively improve effect, but also broaden diversified utilization ways waste, which has a good...

Brucellosis is a notorious zoonotic disease caused by Brucella, which can lead to reproductive diseases in humans and animals, such as infertility abortion. Lipopolysaccharides (LPS) are the main virulence factor of Brucella. LPS derived from Brucella different non-classical less toxic active than isolated E. coli. However, effects possible mechanisms LPS-caused pregnancy loss remain be revealed. In present study, we investigated suis S2 on early mice. The results indicated that embryo...

Agave sisalana is one of the cultivars for sisal fiber production around tropical areas world. In present study, we successfully sequenced and assembled its chloroplast genome. The full size genome 157,268 bp with a GC content at 37.85%. constructed large single copy region (LSC, 85,894 bp), pair inverted repeat regions (IR, 26,573 bp) small (SSC, 18,228 bp). Besides, 86 protein-coding genes, 38 tRNAs 8 rRNAs are annotated on Phylogenetic result reveals that A. closely related americana H11648.