A5103822415- Virus-based gene therapy research
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Cervical Cancer and HPV Research
- Cancer Mechanisms and Therapy
- Autoimmune Neurological Disorders and Treatments
- Reproductive System and Pregnancy
- Viral Infectious Diseases and Gene Expression in Insects
- Cellular Mechanics and Interactions
- RNA modifications and cancer
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- interferon and immune responses
- RNA Research and Splicing
- Cancer, Lipids, and Metabolism
- Cancer-related Molecular Pathways
- Cytokine Signaling Pathways and Interactions
- PI3K/AKT/mTOR signaling in cancer
- Ubiquitin and proteasome pathways
- Studies on Chitinases and Chitosanases
- Hippo pathway signaling and YAP/TAZ
- Mesenchymal stem cell research
- Cholesterol and Lipid Metabolism
- Trigeminal Neuralgia and Treatments
- Cerebral Venous Sinus Thrombosis
Huazhong University of Science and Technology
2011-2025
Tongji Hospital
2011-2025
Yangzhou University
2024
Randall Children's Hospital at Legacy Emanuel
2018-2019
Legacy Health
2018-2019
Legacy Emanuel Medical Center
2019
Cancer Research Center
2014
Mayo Clinic in Florida
2008-2009
Persistent HPV infection is recognized as the main etiologic factor for cervical cancer. expresses oncoproteins E6 and E7, both of which play key roles in maintaining viral promoting carcinogenesis. While siRNA-mediated targeting E7 transcripts temporarily induces apoptosis HPV-positive cells, it does not eliminate DNA within host genome, can harbor escape mutants. Here, we demonstrated that specifically with transcription activator-like effector nucleases (TALENs) apoptosis, inhibits...
Ovarian cancer (OC) progression is an unmet medical challenge. Since omental metastases were palpated harder than their primary counterparts during cytoreductive surgery of patients with epithelial ovarian (EOC), we inspired to investigate OC from the perspective biomechanics.Atomic Force Microscope (AFM) was used measure Young's modulus tissues. The collagen-coated polyacrylamide hydrogel (PA gel) system prepared mimic soft and stiff substrates in vitro. effect TAGLN evaluated both vitro...
Abstract Background P21 (WAF1/Cip1) binds to cyclin-dependent kinase complexes and inhibits their activities. It was originally described as an inhibitor of cancer cell proliferation. However, many recent studies have shown that p21 promotes tumor progression when accumulated in the cytoplasm. So far, little is known about correlation between cytoplasmic drug resistance. This study aimed investigate role cisplatin resistance ovarian cancer. Methods RT-PCR, western blot immunofluorescence...
Cervical cancer is mainly caused by infections of high-risk human papillomavirus (HR-HPV). Persistent expression HR-HPV oncogenes E6 and E7 implicated in malignant transformation. The aim was to provide proof-of-concept data support use zinc finger nucleases (ZFN) targeting HPV treat HPV-related cervical cancer.We designed constructed ZFNs that could specifically recognize cleave HPV16/18 DNA. We tested the cleavage efficiency selected ZFN16-E7-S2 ZFN18-E7-S2 using single-strand annealing...
Oncolytic virotherapy has emerged as a promising strategy for cancer treatment by selectively targeting and lysing tumor cells. However, its efficacy is often limited in certain types due to multiple factors. This study explores the combination of oncolytic adenoviruses with Erastin, potent ferroptosis inducer, enhance antitumor virus-insensitive cell lines. In vitro experiments demonstrated that Erastin significantly increased cytotoxicity virotherapy, leading greater inhibition...
Objectives To investigate the predictive utility of preoperative cervical length (CL) for preterm birth (PTB) in cerclage with different surgical indications.
Objective: Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis predominantly affects children and young women; the disease can have a multistage presentation exhibit wide variety of neuropsychiatric features. This study aimed to investigate profile YKL-40 (Chitinase 3-like 1) in anti-NMDAR patients evaluate its association with modified Rankin Scale (mRS) scores expression inflammatory cytokines. Methods: A total 66 were enrolled this study, 33 encephalitis, 13 viral meningitis 20...
Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is an autoimmune inflammatory brain disease that can develop a variety of neuropsychiatric presentations. However, the underlying nature its neuronal injury remains unclear. Mitochondrial DNA (mtDNA) recently regarded as damage-associated molecular pattern molecule (DAMP) initiate response. In presenting study, we aimed to evaluate levels cell-free mtDNA in cerebrospinal fluid (CSF) patients with anti-NMDAR and determine potential role...
Abstract Background Mesenchymal stem cells (MSCs) have been considered to be the attractive vehicles for delivering therapeutic agents toward various tumor diseases. This study was explore distribution pattern, kinetic delivery of adenovirus, and efficacy MSC loading E1A mutant conditionally replicative adenovirus Adv-Stat3(-) which selectively replicated expressed high levels anti-sense Stat3 complementary DNA in breast cancer melanoma cells. Methods We assessed release ability (CRAd) from...
Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is accepted as an autoimmune disorder of the central nervous system (CNS). NLR family pyrin domain containing 3 (NLRP3) inflammasome, a potent innate inflammatory mediator, can activate IL-1β and induce migration T helper cell into CNS. However, whether NLRP3 inflammasome participates in pathogenesis anti-NMDAR remains unclear. This study aims to determine levels related cytokines (IL-1β, IL-6 IL-17) cerebrospinal fluid (CSF) patients...
Background/Aims: Epithelial ovarian cancer (OC) is the leading cause of death in patients with gynecologic malignancy. Malignant ascites, a shared symptom advanced OC patients, plays an important role peritoneal metastasis cascade OC. Since leptin existed great amount malignant we speculated that it might be involved modulation tumor cells behavior. Keywords: Leptin, cancer, metastasis, EMT, PI3K/Akt/mTOR, therapeutic strategy.
Aim: To investigate the influence of tissue mechanics on cellular uptake efficiency nanoparticles (NPs) in cancer. Materials & methods: Collagen-coated polyacrylamide gels were prepared as model substrates. Coumarin 6-loaded poly(lactic-co-glycolic) acid micelles (C6-NPs) to NPs. Results: We demonstrated that substrate stiffness modulated NPs Mechanistically, mechanical cues exerted clathrin-mediated endocytosis and caveolae-mediated pathways, which mediated stiffness-regulated Conclusion:...
Cervical cancer cells exhibit an increased requirement for ubiquitin-dependent protein degradation associated with elevated metabolic turnover rate. Ubiquitin, which is a small, highly conserved expressed in all eukaryotic cells, can be covalently linked to certain target proteins mark them by the ubiquitin-proteasome system. Previous studies highlight essential role of Ubiquitin B (UbB) and UbB-dependent proteasomal histone deacetylase inhibitor (HDACi) -induced tumor selectivity. We...
Cisplatin-centered chemotherapy is the first-line treatment for human ovarian cancer. However, chemoresistance remains a major obstacle to successful treatment. Evidence has indicated that signal transducer and activator of transcription-3 (STAT3) determinant chemoresistance; it was related tumor recurrence in large number solid malignancies. Unfortunately, none compounds currently developed block STAT3 signaling been considered serious clinical candidate because toxicity or limited...
In this study, we investigated the effect of Realgar on apoptosis HPV16-positive cervical cells in vitro.The was by annexin V-fluorescein isothiocyanate/propidium iodide staining and growth inhibition assays using cancer cell line SiHa immortalized epithelial S12. The expression genes measured real-time PCR, corresponding proteins detected Western blotting. adhesion invasion were assay Transwell assay, respectively.The inhibited proliferation induced S12 a dose- time-dependent manner....
RAD54B, a human paralog of RAD54 L, belongs to the SW12/SNF2 superfamily helicases. Up now, few studies have reported role RAD54B in breast cancer (BC). In this study, we that was amplified and expression elevated BC, as identified through bioinformatics analysis open databases (cBioPortal Oncomine) an immunohistochemical tissue microarray (TMA). Analysis Kaplan-Meier plotter (KM plotter) showed high transcription activity critically linked with poor prognosis BC patients, particular those...
Histone deacetylases (HDACs) have been linked to a variety of cancers, and HDAC inhibitors (HDACi) are promising class drugs that demonstrated anti-cancer effects. However, we little knowledge regarding the selection application personalized treatment ovarian cancer (OC). Here, report correlation between high expression HDACs poor outcomes in OC patients, which reveals HDACi agents show great promise for OC. Furthermore, found increased both mRNA protein levels DHRS2, has shown be closely...
Ubiquinol-cytochrome C reductase core protein II (QCR2) is essential for mitochondrial functions, yet, its role in cancer development has remained elusive.The expression of QCR2 patients was assessed by immunohistochemistry. The proliferation cells CCK-8 assay, EdU staining and Flow cytometry analysis. biological function PHB were determined using western blotting, RT-qPCR, microarray analysis xenografts. interactions between proteins the ubiquitination p53 immunoprecipitation, mass...
The role of histone deacetylases (HDACs) in the tumor immune microenvironment gynecologic tumors remains unexplored. We integrated data from Cancer Genome Atlas and Human Protein to examine HDAC expression breast, cervical, ovarian, endometrial cancers. Elevated correlated with poor prognosis highly malignant cancer subtypes. Gene Set Enrichment Analysis revealed positive associations between proliferation signature, while negative were found inflammation signature. Increased was linked...