A5013350918- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver physiology and pathology
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer, Hypoxia, and Metabolism
- Cancer Mechanisms and Therapy
- Lipid metabolism and disorders
- Retinoids in leukemia and cellular processes
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Epigenetics and DNA Methylation
- Systemic Lupus Erythematosus Research
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Immunotherapy and Immune Responses
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Angiogenesis and VEGF in Cancer
- Ubiquitin and proteasome pathways
- FOXO transcription factor regulation
- Immune cells in cancer
Kanazawa University
2016-2025
Kanazawa University Hospital
2017-2022
Hokkaido University Hospital
2018
Kanazawa Medical University
2012-2015
Hospital de Santa Maria
2015
Centro Hospitalar Lisboa Norte
2015
University of Washington
2012
Kanazawa Hospital
2010
National Institute of Advanced Industrial Science and Technology
2009
The replication and infectivity of the lipotropic hepatitis C virus (HCV) are regulated by cellular lipid status. Among differentially expressed microRNAs (miRNAs), we found that miR-27a was preferentially in HCV-infected liver over B (HBV)-infected liver. Gene expression profiling Huh-7.5 cells showed regulates metabolism targeting synthetic transcription factor RXRα transporter ATP-binding cassette subfamily A member 1. In addition, repressed many metabolism-related genes, including FASN,...
The survival of patients with hepatocellular carcinoma (HCC) is often individually different even after surgery for early-stage tumors. Gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance imaging (MRI) has been introduced recently to evaluate hepatic lesions regard vascularity and the activity organic anion transporter OATP1B3. Here we report that Gd-EOB-DTPA-enhanced MRI (EOB-MRI) in combination serum alpha-fetoprotein (AFP) status reflects...
Background. Although nucleos(t)ide analog (NA) therapy effectively reduces the hepatitis B virus (HBV) DNA load in serum of patients with chronic B, it does not completely reduce incidence hepatocellular carcinoma (HCC).
Significance Hepatocellular carcinoma (HCC) is a highly lethal cancer, partly because of its high rate recurrence, which caused by the presence liver cancer stem cells (CSCs). Here, using selective chemopreventive agent, acyclic retinoid (ACR), as bioprobe, we identified MYCN , mostly recognized an oncogene in neuroblastoma, therapeutic target ACR for HCC through deletion + CSCs. We also demonstrated that expression served prognostic biomarker and positively correlated with recurrence de...
// Kai Takegoshi 1 , Masao Honda 1, 2 Hikari Okada Riuta Takabatake Naoto Matsuzawa-Nagata 3 Jean S. Campbell 4 Masashi Nishikawa Tetsuro Shimakami Takayoshi Shirasaki Yoshio Sakai Taro Yamashita Toshinari Takamura Takuji Tanaka 5 Shuichi Kaneko Department of Gastroenterology, Kanazawa University Graduate School Medicine, Kanazawa, Japan Advanced Medical Technology, Health Disease Control and Homeostasis, Pathology, Washington Seattle, WA, USA The Tohkai Cytopathology Institute: Cancer...
Since hepatocellular carcinoma (HCC) is a typical hypervascular malignant tumor with poor prognosis, targeting angiogenesis an important therapeutic strategy for advanced HCC. Involvement of bone morphologic protein 9 (BMP9), transforming growth factor-beta superfamily member, has recently been reported in the development liver diseases and angiogenesis. Here, we aimed to elucidate role BMP9 signaling promoting HCC assess antiangiogenic effect BMP receptor inhibitors By analyzing tissue gene...
Hepatocellular carcinoma (HCC) often develops in association with liver cirrhosis, and its high recurrence rate leads to poor patient prognosis. Although recent evidence suggests that peretinoin, a member of the acyclic retinoid family, may be an effective chemopreventive drug for HCC, published data about effects on hepatic mesenchymal cells, such as stellate cells endothelial remain limited. Using mouse model which platelet-derived growth factor (PDGF)-C is overexpressed (Pdgf-c Tg),...
Differentially regulated microRNA (miRNA) are associated with hepatic fibrosis; however, their potential usefulness for blocking fibrosis has not been exploited fully. We examined the expression of miRNA in liver a transgenic mouse model which platelet-derived growth factor C (PDGF-C) is overexpressed (Pdgf-c Tg), resulting and steatosis eventual development hepatocellular carcinoma (HCC). Robust induction miR-214 correlated fibrogenesis Pdgf-c Tg mice, atherogenic high-fat diet-induced NASH...
Dendritic cells (DCs) are antigen‐presenting with a central role in host immune response. This study analyzed gene expression and DC function hepatitis B virus (HBV) patients, functions impaired because of HBV, identified the genes related to these functions. Peripheral blood mononuclear from 64 HBV patients 19 healthy controls were analyzed. DCs stained antibodies against human leukocyte antigen–DR/Lin‐1/CD123/CD11c separated into plasmacytoid (pDCs) myeloid by fluorescence‐activated cell...
Cancer stemness evinces interest owing to the resulting malignancy and poor prognosis. We previously demonstrated that hepatic stem cell-like hepatocellular carcinoma (HpSC-HCC) is associated with high vascular invasion Dickkopf-1 (DKK-1), a Wnt signaling regulator, highly expressed in HpSC-HCC. Here, we assessed diagnostic prognostic potential of serum DKK-1. Its levels were significantly higher 391 patients HCC compared 205 chronic liver disease. Receiver operating characteristic curve...
The hepatitis B virus (HBV) infects many people worldwide. As HBV infection frequently leads to liver fibrosis and carcinogenesis, developing anti-HBV therapeutic drugs is urgent. Therapeutic for preventing covalently closed circular DNA (cccDNA) production, which can eliminate infection, are unavailable. host factor dedicator of cytokinesis 11 (DOCK11) involved in the synthesis maintenance cccDNA
Mint3 enhances aerobic ATP production with subsequent nuclear translocation of hypoxia-inducible factor-1 (HIF-1) and activation angiogenesis-related genes. It remains unclear if when is activated whether it involved in hepatocarcinogenesis. We explored the expression surgically resected hepatocellular carcinoma (HCC) tissues. evaluated effects knockdown on spheroid formation capacity subcutaneous tumor growth immune-deficient mice. used knockout mice to evaluate chemically induced HCC...
Pretreatment up-regulation of hepatic interferon (IFN)-stimulated genes (ISGs) has a stronger association with the treatment-resistant interleukin (IL)28B minor genotype (MI; TG/GG at rs8099917) than treatment-sensitive IL28B major (MA; TT rs8099917). We compared expression ISGs in liver and blood 146 patients chronic hepatitis C who received pegylated IFN ribavirin combination therapy. Gene profiles 85 were analyzed using an Affymetrix GeneChip (Affymetrix, Santa Clara, CA). ISG was...
Consumption of n-3 fatty acids reduces the incidence cardiovascular mortality in populations that consume diets rich fish oil. Eicosapentaenoic acid (EPA) is an known to reduce frequency nonfatal coronary events; however, after myocardial infarction (MI) not reduced. The aims this study were determine whether long-term administration EPA regulated cardiac remodeling MI and elucidate underlying therapeutic mechanisms EPA.C57BL/6J mice divided into control (phosphate-buffered saline-treated)...
Malnutrition in the advanced fibrosis stage of chronic hepatitis C (CH-C) impairs interferon (IFN) signaling by inhibiting mammalian target rapamycin complex 1 (mTORC1) signaling. However, effect profibrotic on IFN is not known. Here, transforming growth factor (TGF)-β and virus (HCV) replication was examined Huh-7.5 cells evaluating expression forkhead box O3A (Foxo3a), suppressor cytokine 3 (Socs3), c-Jun, activating transcription 2, ras homolog enriched brain, mTORC1. The findings were...
The malignant nature of hepatocellular carcinoma (HCC) is closely related to the presence cancer stem cells (CSCs). Bone morphologic protein 9 (BMP9), a member transforming growth factor-beta (TGF-β) superfamily, was recently reported be involved in liver diseases including cancer. We aimed elucidate role BMP9 signaling HCC-CSC properties and assess therapeutic effect BMP receptor inhibitors HCC. have identified that high expression tumor tissues or serum from patients with HCC leads poorer...
Background & AimsThe lipid oxidation is a key factor for damaging hepatocytes and causing cell death. However, the mechanisms underlying hepatocyte death role of most popular peroxidation product 4-hydroxy-2-nonenal (HNE) in nonalcoholic steatohepatitis (NASH) remains unclear.MethodsWe demonstrated using hepatoma lines, NASH mouse model, HNE-treated monkeys, biopsy specimens from patients with that HNE induced by disintegrating lysosomal limiting membrane.ResultsThe degree deposition human...
Background: Recent reports have unveiled the potential utility of l -carnitine to alleviate metabolic dysfunction–associated steatohepatitis (MASH) by enhancing mitochondrial function. However, its efficacy at preventing development HCC has not been assessed fully. Methods: (2 g/d) was administered 11 patients with MASH for 10 weeks, and blood liver function tests were performed. Five received a serial biopsy, histology hepatic gene expression evaluated using this tissue. An atherogenic plus...
Sorafenib is a multiple receptor tyrosine kinase inhibitor known to prolong overall survival in patients with advanced hepatocellular carcinoma (HCC). Predicting this drug's benefits challenging because clinical responses are rarely measurable during treatment. In study, we hypothesized that serum cytokines levels could predict the of HCC patients, as sorafenib targets signaling pathways activated tumor stromal microenvironment and potentially affects cytokine profiles.Of 143 advanced-stage...
Cancer stem cells (CSCs) are a pivotal target for eradicating hepatocellular carcinoma (HCC). We previously reported that distinctive CSCs regulating tumorigenicity (EpCAM+ CSCs) and metastasis (CD90+ have different epithelial/mesenchymal gene expression signatures. Here, we examined the influence of sorafenib, multiple-receptor tyrosine kinase inhibitor used as first-line treatment advanced HCC, on EpCAM+ CD90+ CSCs. showed higher c-Kit gene/protein than cells. Sorafenib reduced number with...
Abstract Sphingosine-1-phospate is a potent bioactive lipid metabolite that regulates cancer progression. Because sphingosine kinase 1 and 2 (SPHK 1/2) are both essential for sphingosine-1-phospate production, they could be therapeutic target in various cancers. Peretinoin, an acyclic retinoid, inhibits post-therapeutic recurrence of hepatocellular carcinoma via unclear mechanisms. In this study, we assessed effects peretinoin on SPHK expression liver development vitro vivo . We examined...
Osteopontin (OPN) is involved in cell proliferation, migration, inflammation, and tumor progression various tissues. OPN induces stemness by interacting with CD44, but the functional relevance of OPN-mediated interferon (IFN) signaling hepatitis C virus (HCV) replication stem populations remains unclear. In this study, we investigated effect on HCV IFN cancer cells (CSCs) positive for epithelial adhesion molecule (EpCAM) CD44. We show that EpCAM+/CD44+ CSCs marked when compared to...