Abstract Introduction Osteoarthritis (OA) is an inflammatory disease of the joints that causes progressive disability in elderly. Reactive oxygen species (ROS) play important role OA development; they may activate NLRP3 inflammasome, thereby inducing secretion proinflammatory IL-1β and IL-18, leading to aggravation downstream response. Nrf2 a key transcription factor regulates expression antioxidant enzymes protect against oxidative stress tissue damage. We aimed explore underlying mechanism...

Osteoporosis (OP) is the most common bone disorder worldwide, especially in postmenopausal women. However, many OP drugs are not suitable for long term use due to major adverse effects. Therefore, there an urgent need identify more effective and safe therapeutic drugs. Pueraria lobata has been reported promote osteoblast growth regeneration, but exact mechanisms still further exploration. The current study found that lobata-derived exosome-like nanovesicles (PELNs) promoting primary human...

Osteoporosis (OP) is a prevalent metabolic bone disease characterized by loss and structural deterioration, which increases the risk of fracture especially in older people. Recent research has shown that gut microbes play an important role OP. Trimethylamine N-oxide (TMAO), microbiota-derived metabolite, been implicated pathogenesis diseases, including Alzheimer's cerebrovascular disease. This study aimed to examine effect TMAO OP.In this study, we firstly investigated relationship between...

Introduction: Osteosarcoma is a prevalent and highly malignant primary bone tumor. However, current clinical therapeutic drugs for osteosarcoma are not suitable long-term use due to significant side effects. Therefore, there an urgent need develop new with fewer Dipsacus asperoides C. Y. Cheng et T. M. Ai, traditional Chinese medicine, commonly used its anti-inflammatory, anti-pain, fracture healing, anti-tumor In this study, we investigated the effects of exosome-like nanoparticles derived...

Abstract: Apoptosis of osteoblasts triggered by high-dose glucocorticoids (GCs) has been identified as a major cause osteoporosis. However, the underlying molecular mechanisms accounting for this action remain elusive, which impeded prevention and cure side effect. Sulforaphane (SFP) is naturally occurring isothiocyanate that huge health benefits humans. In study, using osteoblastic MC3T3-E1 cells model, we demonstrate protective effects SFP against dexamethasone (Dex)-induced apoptosis...

Although the long non-coding RNA THOR has been reported to promote cancer stem cell expansion in liver and gastric cancer, its effects on osteosarcoma (OS) cells remain unclear. Here, we investigated roles of stemness migration OS cells. We report that level is remarkably upregulated spheroids compared adherent overexpression increased spheroid formation ability aldehyde dehydrogenase 1 (ALDH1) activity cells, opposite effect was observed with knockdown. Additionally, formed by exhibited a...

Breast cancer (BRCA) is the most common in women, while bones are one of sites metastasis. Although new diagnostic methods or radiation chemotherapies and targeted therapies have made huge advances, occurrence bone metastasis also linked with poorer survival. Enhancer RNAs (eRNAs) been demonstrated to participate progression tumorigenesis However, role eRNAs BRCA remains largely unclear.Gene expression profiling 1,211 primary 17 metastases samples were retrieved from The Cancer Genome Atlas...

The histone methyltransferase EZH2 (enhancer of zeste homolog 2) plays critical roles in prostate cancer (PCa) development and is a potential target for PCa treatment. Triptolide possesses anti-tumor activity, but it unknown whether its therapeutic effect relates with PCa. Here we described as cells. Our data showed that suppressed cell growth reduced the expression EZH2. Overexpression attenuated induced inhibition. Moreover, treatment PC-3 cells resulted elevated mRNA levels genes (ADRB2,...

Osteoporosis, a critical public health challenge, is marked by skeletal deformities and heightened fracture risk. Steleophaga plancyi (Boleny) (SP), component of traditional Chinese medicine, known to enhance bone health, but the molecular mechanisms behind its osteoprotective effects are not well understood. We isolated extracellular vesicle (EV)-like particles from SP (SP-EVLP) using differential velocity centrifugation investigated their on human marrow stromal cells (hBMSCs) in vitro....

<title>Abstract</title> <bold>Backgroud </bold>Alzheimer's disease (AD) is a chronic, progressive, and destructive neurodegenerative disorder that severely affects human memory, intelligence, behavioral abilities. Jiao Tai Wan (JTW) classic formula composed of two traditional Chinese medicines, coptis chinensis (CC) cinnamon (CIN), the ratio CC CIN 10:1. JTW has effects promoting cognitive function, improving learning memory function. But specific mechanism not been systematically studied....

The zebrafish (Danio rerio) has recently been shown to be an ideal model study bone disease including osteoporosis. osteoporosis could induced by glucocorticoid treatment with chemical staining for reflecting the level of mineralization. However, this methodology was unstable. Here, we developed a novel directly evaluate mass and density.We generated used transgenic Tg (ola.sp7:nlsGFP) density measuring areal extent integrated optical (IOD) enhanced green fluorescent protein (eGFP). This...

Tumor angiogenesis plays a vital role in tumorigenesis, proliferation, and metastasis. Recently, vascular endothelial growth factor A (VEGFA) CXC chemokines have been shown to play roles angiogenesis. Exploring the expression level, gene regulatory network, prognostic value, target prediction of chemokine-VEGFA network colon adenocarcinoma (COAD) is crucial from perspective tumor In this study, we analyzed regulation, prediction, immune infiltrates related patients with COAD using multiple...

The extracellular microvesicles (MVs) are attracting much attention because they found to be the key paracrine mediator participating in tissue regeneration. Dexamethasone (DXM) is widely accepted as an important regulator tailoring differentiation potential of mesenchymal stem cells (MSCs). However, effect DXM on signaling MSCs remains unknown. To this point, we aimed explore role regulating activity through evaluating release and function MSC-MVs, based their physicochemical...

Background . The functions of insulin in mesenchymal stem cells (MSC) remain poorly understood. Methods. MSC from human umbilical cord matrix (UCM) cultured serum-free media (SFM) with or without were subjected to various molecular biological analyses determine their proliferation and growth states, expression levels Akt-cyclin D1 signaling molecules, vitro differentiation capacities. Results. Insulin accelerated the G1-S cell cycle progression UCM-MSC significantly stimulated SFM....

Objective: To evaluate whether EPC-MVs could promote bone regeneration by directly regulating osteoblast through miR-126. The underlying mechanisms were also explored. Methods: EPCs isolated from marrow mononuclear cells. collected cultured medium. lentivirus was used to induce miR-126 over-expression in and EPC-MVs. expression detected qRT-PCR. proliferation, migration, apoptosis differentiation abilities of cells MC3T3-E1 analysed the presence or absence overexpressed (EPC-MVs-miR126)....

Osteosarcoma (OS) is the most common type of malignant bone tumor that affects children and adolescents.Still, cellular molecular mechanisms driving development this disease remain poorly understood.In study, numerous dysregulated lncRNAs were identified by RNA-seq.As a result, we able to find novel lncRNA Lnc-MAP6-1:3 which highly expressed in osteosarcoma.Using set approaches including gene knockdown, RT-PCR, oncogenic function assay western blotting, observed knockdown expression...

Background: With the continued advancement of RNA-seq (RNA-sequencing), microRNA (miRNA) editing events have been demonstrated to play an important role in different malignancies. However, there is yet no description miRNA recurrent bladder cancer. Objective: To identify and compare primary cancer, as well investigate potential molecular mechanism its impact on patient prognosis. Methods: We examined mRNA transcriptomes 12 cancer cases 13 cases. The differentially expressed sequences were...

Osteosarcoma is prone to metastasis and has a low long-term survival rate. The drug treatment of osteosarcoma, side effects drugs, prognosis patients with lung continue present significant challenges, the efficacy drugs used in osteosarcoma remains low. development new therapeutic urgently needed. In this study, we successfully isolated Pinctada martensii mucilage exosome-like nanovesicles (PMMENs). Our findings demonstrated that PMMENs inhibited viability proliferation 143B cells, induced...

The aim of the present study was to investigate correlation between glucocorticoid activity regulation, prostaglandin E2 (PGE2) synthesis, and synovial inflammation inhibition activity, through microsomal E synthase-1 (mPGES-1) expression regulated by pre-receptor regulator, 11β-hydroxysteroid dehydrogenase-1 (11β-HSD1). In study, fibroblast-like cells rats were studied as a cell model. Cells stimulated with 10 ng/ml interleukin (IL)-1β for 24 h, subsequently, within next treated or without...

Troponin T1 (TNNT1) plays a crucial role in muscle contraction but its cancer, particularly kidney renal clear cell carcinoma (KIRC), is not well-understood. This study explores the expression, clinical significance and biological functions of TNNT1 various cancers, with an emphasis on involvement KIRC. We analysed expression cancers using databases like TCGA GTEx, assessing prognostic value, mutation patterns, methylation status functional implications. The also examined TNNT1's effect...

OBJECTIVE Nuclear factor erythroid-2 related 2 (Nrf2)/ antioxidant response element (ARE) is a novel defensive pathway involved in the oxidative and chemical stress of cells. The aim study was to explore role Nrf2 on apoptosis human disc nucleus pulpous cells induced by hydrogen peroxide (H2O2). METHODS degeneration model intervertebral established. expression interfered with using sulforaphane (SFN); for that end, three groups were established: blank group (H2O2-/SFN-), control...

Background: Osteosarcoma (OS) is the most common primary malignant bone tumour in children and adolescents, with rapid growth, frequent metastasis, a poor prognosis, but its pathogenesis has not been fully elucidated. Exploring of OS great significance for improving diagnoses finding new therapeutic targets. Methods: Differentially expressed circRNAs (DECs), miRNAs (DEMs), methylated DNA sites (DMSs), mRNAs (DEGs) were identified between control cell lines. GSEA DEGs functional enrichment...