A5081272210- Reproductive Biology and Fertility
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Assisted Reproductive Technology and Twin Pregnancy
- Mitochondrial Function and Pathology
- Reproductive System and Pregnancy
- Ovarian function and disorders
- Reproductive Health and Technologies
- Metabolism and Genetic Disorders
- Prenatal Screening and Diagnostics
- Renal and related cancers
- Chromosomal and Genetic Variations
- Sexual Differentiation and Disorders
- Animal Genetics and Reproduction
- Reproductive biology and impacts on aquatic species
- Glycosylation and Glycoproteins Research
- Gynecological conditions and treatments
- RNA and protein synthesis mechanisms
- Anesthesia and Neurotoxicity Research
- Liver physiology and pathology
- Pregnancy and preeclampsia studies
- Adipose Tissue and Metabolism
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Selenium in Biological Systems
Fudan University
2016-2024
Obstetrics and Gynecology Hospital of Fudan University
2014-2022
ShangHai JiAi Genetics & IVF Institute
2012-2021
Genetics and IVF Institute
2019
Shanghai Medical College of Fudan University
2015
Early embryonic arrest is a challenge for in vitro fertilization (IVF). No genetic factors were previously revealed the sperm-derived of development. Here, we reported two infertile brothers presenting normal conventional semen analysis, but both couples had no embryos transfer after several IVF and intracytoplasmic sperm injection (ICSI). Whole-exome sequencing identified homozygous missense mutation ACTL7A brothers. This deleterious causes acrosomal ultrastructural defects. The Actl7a...
Abstract Background MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown the expression levels total miRNAs increase during mouse embryonic development, and some control regulatory network development progression. However, few focused on effects early human development. The relationship between embryogenesis is still unknown. Results In this study, RNA-seq data collected...
Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of reproductive age, and oocyte developmental competence altered patients with PCOS. In recent years microRNAs (miRNAs) have emerged as important regulators gene expression, the aim study was to miRNAs expression patterns cumulus cells from PCOS patients. The included 20 undergoing vitro fertilization (IVF) intra-cytoplasmic sperm injection (ICSI): 10 diagnosed matching controls. We used deep sequencing technology...
Abstract Prohibitin ( PHB ), a major mitochondrial membrane protein, has been shown earlier in our laboratoryto regulate sperm motility via an alteration potential MMP ) infertile men with poor quality. To test if expression is associated superoxide mROS levels, here we examined high and lipid peroxidation (asthenospermia, A) and/or low concentrations (oligoasthenospermia, OA ). The diaphorase‐type activity of complex I MCI were also determined. We demonstrate that levels are significantly...
Manual semen evaluation methods are subjective and time-consuming. In this study, a deep learning algorithmic framework was designed to enable non-invasive multidimensional morphological analysis of live sperm in motion, improve current clinical morphology testing methods, significantly contribute the advancement assisted reproductive technologies. We improved FairMOT tracking algorithm by incorporating distance angle same head movement adjacent frames, as well target detection frame IOU...
Sperm motility is essential for male reproduction or natural fertilization. The cyclic AMP (cAMP)/cAMP-dependent protein kinase A (PKA) signaling pathway generally recognized as one of the significant pathways in regulation mammalian spermatozoan motility. Since Zn-alpha2-glycoprotein (ZAG) activity adipose tissue mediated via beta(3)-adrenoreceptor, with upregulation cAMP pathway, we hypothesize that ZAG may play same role sperm regulation, a new factor Therefore, gene encoding human was...
Prohibitin (PHB) is a highly conserved major sperm mitochondrial membrane protein whose absence in somatic cells associated with depolarization and increased generation of reactive oxygen species (ROS). Our recent findings suggest that high levels oxidants human semen may contribute to male infertility motility could be the earliest most sensitive indicator oxidative damage. Based on PHB's roles sub-compartmentalization respiratory chain assembly, we examine PHB expression potential (MITO)...
It is well known that cell surface glycans or glycocalyx play important roles in sperm motility, maturation and fertilization. A comprehensive profile of the will greatly facilitate both basic research (sperm glycobiology) clinical studies, such as diagnostics infertility. As a group natural glycan binders, lectin an ideal tool for profiling. However, because lack effective technology, only few lectins have been tested lectin-sperm binding profiles. To address this challenge, we developed...
Abstract Coating on the sperm surface, glycocalyx, plays a key role in motility, maturation and fertilization. A comprehensive profile of surface glycans will greatly facilitate both basic researches clinical studies. Because capability recognizing different glycan moieties, lectins are widely used glycobiology. However, lacking high-throughput technology, limited have been reported for analyzing human sperm. In this study, we employed lectin microarray profiling sperm, which 54 out 91...
Clinical ovulation induction induces blood estrogen (E2) in excess of physiological levels, which can hinder uterine receptivity. In contrast, progesterone produces the opposite clinical effect, suggesting that it might be capable recovering lost receptivity resulting from exposure to high levels. Integrins are most widely used biological markers for monitoring conditions. We studied progesterone-induced changes integrin β expression patterns as biomarkers response increased Endometrial...
Obtaining high-quality embryos is one of the key factors to improve clinical pregnancy rate assisted reproductive technologies (ART). So far, evaluation embryo quality depends on morphology. However, still low. Therefore, new indicators are needed further quality. Several studies have shown that decrease sperm-specific protein actin-like 7A (ACTL7A) leaded low fertilization rate, poor development, and even infertility. The aim this study was whether different expression levels ACTL7A sperm...
Abstract DEFB 126 rs140685149 mutation was shown to cause sperm dysfunction and subfertility. Indel rs11467497 is another 4‐nucleotide frame‐shift (151bp upstream of rs140685149) that leads the premature termination translation expression peptide truncated at carboxyl terminus. In present study, we performed a comprehensive association study check contribution male infertility. Our results confirmed previous findings there no between motility. contrast, found significant indel with Moreover,...
Prohibitin (PHB), an evolutionarily conserved mitochondrial inner membrane protein, is highly expressed in cells that require strong function. Recently, we demonstrated the deletion of Phb spermatocytes results impaired In addition, PHB expression sheath human sperm has a significantly negative correlation with reactive oxygen species levels, but positive one potential and motility. These suggest plays role However, mechanism PHB-mediated regulation motility remains unknown. Here,...
Abstract Tamoxifen is administered for estrogen receptor positive (ER + ) breast cancers, but it can induce uterine endometrial cancer and non-alcoholic fatty liver disease (NAFLD). Importantly, ten years of tamoxifen treatment has greater protective effect against ER than five such treatment. was also approved by the FDA as a chemopreventive agent those deemed at high risk development cancer. The side effects are substantial concern because these extended methods administration. In this...
Semen cryopreservation has been widely applied in assisted reproductive technologies and sperm bank, but it causes considerable impairments on quality. It is necessary to find an evaluation indicator for determining the sperm-freezing tolerance.The glycocalyx of good freezability ejaculates was compared with poor by lectin microarray. The significant different lectins were validated flow cytometry (FACS). To analyze relationship between potential biomarker tolerance cryopreservation, 60...
Objective: To evaluate the effect of preimplantation genetic testing for aneuploidy (PGT-A) in infertile patients with recurrent pregnancy loss (RPL). Methods: A prospective randomized clinical trial was performed a university-affiliated fertility center Shanghai, China. Patients PGT-A group underwent blastocyst biopsy followed by single-nucleotide polymorphism microarray-based and single euploid transfer, whereas control routine vitro fertilization/ICSI procedures frozen embryo transfer 1-2...
Objective This was a prospective study to investigate whether progesterone affects sperm activity by regulating the cyclic AMP-protein kinase A (cAMP-PKA) signalling pathway via α/β hydrolase domain-containing protein 2 (ABHD2). Methods Spermatozoa were collected from healthy and infertile men (with oligoasthenospermia or abnormal acrosome; n = 30/group). The expression of mutations in ABHD2 detected quantitative PCR, western blot, gene sequencing. presence all groups, cAMP PKA levels ELISA...
Objective To investigate female etiology of infertility in assisted reproductive clinic recent years. Methods A retrospective analysis was conducted on the clinical data 23 317 patients diagnosed Shanghai Ji Ai Genetics and IVF Institute from 2013 to 2017. According current guidelines standards, cases (including factor bilateral infertility) integrated analyzed. Results According analysis, factor, male unexplained accounted for 62.6%, 9.2%, 26.5% 1.7% total respectively. The...
Abstract Background : MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown the expression levels total miRNAs increase during mouse embryonic development, and some control regulatory network development progression. However, few focused on effects early human development. The relationship between embryogenesis is still unknown. Results: In this study, sperm samples from 102...
Abstract Background: MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown the expression levels total miRNAs increase during mouse embryonic development, and some control regulatory network development progression. However, few focused on effects early human development. The relationship between embryogenesis is still unknown. Results:In this study, RNA-seq data collected...